scholarly journals Rescuing Dicer expression in inflamed colon tissues alleviates colitis and prevents colitis-associated tumorigenesis

Theranostics ◽  
2020 ◽  
Vol 10 (13) ◽  
pp. 5749-5762
Author(s):  
Xiaoli Wu ◽  
Xiao Chen ◽  
Hui Liu ◽  
Zhong-Wei He ◽  
Zheng Wang ◽  
...  
Keyword(s):  
Dicer Expression

Prognostic significance of Dicer expression in ovarian cancer—link to global microRNA changes and oestrogen receptor expression

2009 ◽  
pp. n/a-n/a ◽  
Author(s):  
Areeg Faggad ◽  
Jan Budczies ◽  
Oleg Tchernitsa ◽  
Silvia Darb-Esfahani ◽  
Jalid Sehouli ◽  
...  
Keyword(s):  
Oestrogen Receptor ◽  
Prognostic Significance ◽  
Receptor Expression ◽  
Dicer Expression

Downregulation of Dicer expression by serum withdrawal sensitizes human endothelial cells to apoptosis

2008 ◽  
Vol 295 (6) ◽  
pp. H2512-H2521 ◽  
Author(s):  
Satoshi Asada ◽  
Tomosaburo Takahashi ◽  
Koji Isodono ◽  
Atsuo Adachi ◽  
Hiroko Imoto ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Caspase 3 ◽  
Umbilical Vein ◽  
Vascular Endothelial Cell ◽  
Serum Withdrawal ◽  
Tyrosine Kinase 2 ◽  
Nitric Oxide Synthase 3 ◽  
Induced Apoptosis ◽  
Dicer Expression ◽  
Umbilical Vein Endothelial Cells

Although the modulated expression of Dicer is documented upon neoplastic transformation, little is known of the regulation of Dicer expression by environmental stimuli and its roles in the regulation of cellular functions in primary cells. In this study, we found that Dicer expression was downregulated upon serum withdrawal in human umbilical vein endothelial cells (HUVECs). Serum withdrawal induced a time-dependent repression of Dicer expression, which was specifically rescued by vascular endothelial cell growth factor or sphingosine-1-phosphate. When Dicer expression was silenced by short-hairpin RNA against Dicer, the cells were more prone to apoptosis under serum withdrawal, whereas the rate of apoptosis was comparable with control cells in the serum-containing condition. Real-time PCR-based gene expression profiling identified several genes, the expression of which was modulated by Dicer silencing, including adhesion and matrix-related molecules, caspase-3, and nitric oxide synthase 3 (NOS3). Dicer silencing markedly impaired migratory functions without affecting cell adhesion and repressed phosphorylation of focal adhesion kinase and proline-rich tyrosine kinase 2 in adherent HUVECs. Dicer knockdown upregulated caspase-3 and downregulated NOS3 expression, and serum withdrawal indeed increased caspase-3 and decreased NOS3 expression. Furthermore, the overexpression of Dicer in HUVECs resulted in a marked reduction in apoptosis upon serum withdrawal and a decreased caspase-3 and increased NOS3 expression. The inhibition of NOS activity by Nω-nitro-l-arginine methyl ester abrogated the effect of Dicer overexpression to rescue the cells from serum withdrawal-induced apoptosis. These results indicated that serum withdrawal decreases Dicer expression, leading to an increased susceptibility to apoptosis through the regulation of caspase-3 and NOS3 expression.

scholarly journals Prognostic Significance of Deregulated Dicer Expression in Breast Cancer

PLoS ONE ◽  
2013 ◽  
Vol 8 (12) ◽  
pp. e83724 ◽  
Author(s):  
Emer Caffrey ◽  
Helen Ingoldsby ◽  
Deirdre Wall ◽  
Mark Webber ◽  
Kate Dinneen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Prognostic Significance ◽  
Dicer Expression

scholarly journals Overexpressed miR-200a promotes bladder cancer invasion through direct regulating Dicer/miR-16/JNK2/MMP-2 axis

Oncogene ◽  
2019 ◽  
Vol 39 (9) ◽  
pp. 1983-1996 ◽  
Author(s):  
Rui Yang ◽  
Jiheng Xu ◽  
Xiaohui Hua ◽  
Zhongxian Tian ◽  
Qipeng Xie ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cell Invasion ◽  
Biological Significance ◽  
Protein Translation ◽  
Mechanistic Studies ◽  
Mrna Transcription ◽  
Ongoing Development ◽  
Muscle Invasive ◽  
Dicer Expression ◽  
New Strategies

AbstractInvasive bladder cancer (BC) is one of the most lethal malignant urological tumors. Although miR-200a has been reported as an onco-miRNA that targets the PTEN gene in endometrioid carcinoma, its biological significance in BC invasion has been poorly explored. In the current study, we found that miR-200a was markedly overexpressed in both human BC tissues and BBN-induced muscle-invasive BC tissues. We further showed that miR-200a overexpression specifically promoted human BC cell invasion, but not migration, via transcriptional upregulation of matrix metalloproteinase (MMP)-2. Mechanistic studies indicated that the increased phosphorylation of c-Jun mediated the increasing levels of MMP-2 mRNA transcription. Further investigation revealed that Dicer was decreased in miR-200a overexpressed BC cells; this resulted in inhibition of miR-16 maturation and consequently led to increased JNK2 protein translation and c-Jun activation. Taken together, the studies here showed that miR-200a overexpression inhibited Dicer expression, in turn, resulted in inhibition of miR-16 maturation, leading to upregulation of JNK2 expression, c-Jun phosphorylation, MMP-2 transcription and, ultimately, BC invasion. Collectively, these results demonstrate that miR-200a is an onco-miRNA that is a positive regulator for BC invasion. This finding could be very useful in the ongoing development of new strategies to treat invasive BC patients.

Epithelial-Mesenchymal Transition and Metastasis: Role of Dicer Expression

2012 ◽  
pp. 213-229 ◽  
Author(s):  
Stéphanie Courtois-Cox ◽  
Caroline Moyret-Lalle
Keyword(s):  
Epithelial Mesenchymal Transition ◽  
Mesenchymal Transition ◽  
Dicer Expression

Abstract B68: Calorie restriction normalizes global microRNA expression by preventing the loss of Dicer expression during mammary tumorigenesis

2016 ◽  
Author(s):  
Kaylyn L. Devlin ◽  
Tiffany Sanford ◽  
Elizabeth Mambo ◽  
Stephen D. Hursting
Keyword(s):  
Calorie Restriction ◽  
Mammary Tumorigenesis ◽  
Microrna Expression ◽  
Dicer Expression

Abstract PR07: Calorie restriction normalizes global microRNA expression by preventing the loss of Dicer expression during mammary tumorigenesis

2015 ◽  
Author(s):  
Kaylyn L. Devlin ◽  
Tiffany Sanford ◽  
Elizabeth Mambo ◽  
Stephen D. Hursting
Keyword(s):  
Calorie Restriction ◽  
Mammary Tumorigenesis ◽  
Microrna Expression ◽  
Dicer Expression

scholarly journals Letter to the Editor: Does Dicer Expression Affect shRNA Processing?

2009 ◽  
Vol 3 ◽  
pp. GRSB.S2551
Author(s):  
Neil Senzer ◽  
Donald Rao ◽  
John Nemunaitis
Keyword(s):  
Letter To The Editor ◽  
Dicer Expression

scholarly journals Dicer Expression Exhibits a Tissue-Specific Diurnal Pattern That Is Lost during Aging and in Diabetes

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e80029 ◽  
Author(s):  
Yuanqing Yan ◽  
Tatiana E. Salazar ◽  
James M. Dominguez ◽  
Dung V. Nguyen ◽  
Sergio Li Calzi ◽  
...  
Keyword(s):  
Diurnal Pattern ◽  
Tissue Specific ◽  
Dicer Expression

Calcitriol increases Dicer expression and modifies the microRNAs signature in SiHa cervical cancer cells

2015 ◽  
Vol 93 (4) ◽  
pp. 376-384 ◽  
Author(s):  
Ramiro José González-Duarte ◽  
Verna Cázares-Ordoñez ◽  
Sandra Romero-Córdoba ◽  
Lorenza Díaz ◽  
Víctor Ortíz ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Vitamin D ◽  
Cancer Cells ◽  
Cancer Biology ◽  
Response Element ◽  
Cancer Pathways ◽  
Siha Cells ◽  
Cervical Cancer Cells ◽  
Dicer Expression

MicroRNAs play important roles in cancer biology. Calcitriol, the hormonal form of vitamin D3, regulates microRNAs expression in tumor cells. In the present study we asked if calcitriol would modify some of the components of the microRNA processing machinery, namely, Drosha and Dicer, in calcitriol-responsive cervical cancer cells. We found that calcitriol treatment did not affect Drosha mRNA; however, it significantly increased Dicer mRNA and protein expression in VDR-positive SiHa and HeLa cells. In VDR-negative C33-A cells, calcitriol had no effect on Dicer mRNA. We also found a vitamin D response element in Dicer promoter that interacts in vitro to vitamin D and retinoid X receptors. To explore the biological plausibility of these results, we asked if calcitriol alters the microRNA expression profile in SiHa cells. Our results revealed that calcitriol regulates the expression of a subset of microRNAs with potential regulatory functions in cancer pathways, such as miR-22, miR-296-3p, and miR-498, which exert tumor-suppressive effects. In summary, the data indicate that in SiHa cells, calcitriol stimulates the expression of Dicer possibly through the vitamin D response element located in its promoter. This may explain the calcitriol-dependent modulation of microRNAs whose target mRNAs are related to anticancer pathways, further adding to the various anticancer mechanisms of calcitriol.

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