scholarly journals CYTOTOXIC EFFECT OF NEWLY SYNTHESIZED NANOMATERIALS FOR POTENTIAL DENTAL APPLICATION

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Ognjenka Janković ◽  
Radmila Arbutina ◽  
Vladan Mirjanić
Keyword(s):  
Calcium Aluminate ◽  
Calcium Silicate ◽  
Cytotoxic Effect ◽  
Essential Feature ◽  
Human Fibroblasts ◽  
High Survival ◽  
Mean Values ◽  
Mtt Test ◽  
Human Line ◽  
Dental Applications

Introduction: Biocompatibility is an essential feature of any dental material. Few materials can be said to be biologically inert since most contain potentially harmful or irritating ingredients. This study aimed to determine the cytocompatibility of newly synthesized nanomaterials based on calcium aluminates and calcium silicates for potential dental applications. Material and methods: The cytotoxicity of calcium aluminate-based nanomaterials (ALBO-CA), calcium silicate (ALBO-CS) and calcium silicate hydroxyapatite (ALBO-CSHA) was examined using the MTT test on the human line of human fibroblasts (MRC-5) according to ISO standard (ISO 10993- 5: 2009) in comparison with the calcium aluminate cements EndoBinder (Binder was, São Carlos, SP, Brazil). For the analysis, the eluates of the investigated materials in the growth medium were diluted to a concentration of 4.7, 9.4, 18.8, 37.5 and 75, 0 mg. Qualitative verification of results was performed by a light microscope (Carl Zeiss). The mean values and standard deviations of the MTT test results were done in Microsoft Excel. Results: All tested concentrations of ALBO-CA, ALBO-CS, and EndoBinder resulted in a high survival of cells in culture. The strongest cytotoxic effect was ALBO-CSHA with IC50 = 46.44 after the first cycle of testing; IC50 = 55.52 after the second cycle; or IC50 = 55.42 after the third repetition of the MTT test. Conclusion: ALBO-CA and ALBO-CS nanomaterials have shown a cytocompatible effect comparable to EndoBinder. The obtained results are certainly encouraged to continue researching these materials in the future and other experimental and clinical studies.

The capture of carbon dioxide by transition metal aluminates, calcium aluminate, calcium zirconate, calcium silicate and lithium zirconate

2011 ◽  
Vol 5 (4) ◽  
pp. 477-491 ◽  
Author(s):  
Ganesh Tilekar ◽  
Kiran Shinde ◽  
Kishor Kale ◽  
Reshma Raskar ◽  
Abaji Gaikwad
Keyword(s):  
Carbon Dioxide ◽  
Transition Metal ◽  
Calcium Aluminate ◽  
Calcium Silicate ◽  
Calcium Zirconate ◽  
Lithium Zirconate

scholarly journals Shear bond strength of novel calcium aluminate-based cement (EndoBinder) to root dentine

2014 ◽  
Vol 08 (04) ◽  
pp. 498-503 ◽  
Author(s):  
Lucas da Fonseca Roberti Garcia ◽  
Hebert Luis Rossetto ◽  
Fernanda de Carvalho Panzeri Pires-de-Souza
Keyword(s):  
Bond Strength ◽  
Calcium Aluminate ◽  
Shear Bond Strength ◽  
Testing Machine ◽  
Mineral Trioxide Aggregate ◽  
Acrylic Resin ◽  
Promising Alternative ◽  
Mean Values ◽  
Significant Difference ◽  
Molar Teeth

ABSTRACT Objective: To evaluate the shear bond strength of a novel calcium aluminate-based cement, EndoBinder (EB), to dentine in comparison with Grey and White Mineral Trioxide Aggregate (MTA). Materials and Methods: Root canal hemi-sections obtained from 30 extracted molar teeth were embedded in self-polymerized acrylic resin and were grounded wet in order to obtain a flat dentine surface. Next, the roots were randomly assigned into three groups (n = 10), according to the cement used, as follows: EB: EndoBinder; WMTA: White MTA and GMTA: Grey MTA. The shear bond strength test was performed using a Universal Testing Machine (0.5 mm/min) and the data were submitted to statistical analysis (1-way ANOVA and Tukey tests, P < 0.05). Results: EB presented the highest shear bond strength values; however, there was no statistically significant difference in comparison with GMTA (P > 0.05). WMTA presented the lowest mean values, which were significant in comparison with EB (P < 0.05). Conclusions: The novel calcium aluminate-based cement presented higher shear bond strength than WMTA, and should be considered as a promising alternative in endodontic therapy.

scholarly journals Information Length as a Useful Index to Understand Variability in the Global Circulation

Mathematics ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 299 ◽  
Author(s):  
Eun-jin Kim ◽  
James Heseltine ◽  
Hanli Liu
Keyword(s):  
Climate Model ◽  
Traditional Approach ◽  
Essential Feature ◽  
Complex Form ◽  
Time Dependent ◽  
Spatial Gradient ◽  
Mean Values ◽  
Global Circulation ◽  
New Perspective ◽  
Non Gaussian

With improved measurement and modelling technology, variability has emerged as an essential feature in non-equilibrium processes. While traditionally, mean values and variance have been heavily used, they are not appropriate in describing extreme events where a significant deviation from mean values often occurs. Furthermore, stationary Probability Density Functions (PDFs) miss crucial information about the dynamics associated with variability. It is thus critical to go beyond a traditional approach and deal with time-dependent PDFs. Here, we consider atmospheric data from the Whole Atmosphere Community Climate Model (WACCM) and calculate time-dependent PDFs and the information length from these PDFs, which is the total number of statistically different states that a system evolves through in time. Specifically, we consider the three cases of sampling data to investigate the distribution of information (information budget) along the altitude and longitude to gain a new perspective of understanding variabilities, correlation among different variables and regions. Time-dependent PDFs are shown to be non-Gaussian in general; the information length tends to increase with the altitude albeit in a complex form; this tendency is more robust for flows/shears than temperature. Much similarity among flows and shears in the information length is also found in comparison with the temperature. This means a strong correlation among flows/shears because of their coupling through gravity waves in this particular WACCM model. We also find the increase of the information length with the latitude and interesting hemispheric asymmetry for flows/shears/temperature, with the tendency of anti-correlation (correlation) between flows/shears and temperature at high (low) latitude. These results suggest the importance of high latitude/altitude in the information budget in the Earth’s atmosphere, the spatial gradient of the information length being a useful proxy for information flow.

Novel Fluoride-doped Calcium-silicate cement for dental applications. The effect of Bi2O3 and BaSO4 radiopacifier agents on setting time

2010 ◽  
Vol 150 ◽  
pp. 465-465
Author(s):  
Maria Giovanna Gandolfi ◽  
Andrea Colin ◽  
Giovanni Luca Acquaviva ◽  
Stefano Chersoni ◽  
Fabio Fava ◽  
...  
Keyword(s):  
Calcium Silicate ◽  
Setting Time ◽  
Calcium Silicate Cement ◽  
Dental Applications

scholarly journals Characterization of cytotoxic effect of asbestos on normal human fibroblasts.

1983 ◽  
Vol 51 ◽  
pp. 237-239 ◽  
Author(s):  
M J Chang ◽  
N P Singh ◽  
A Turturro ◽  
R W Hart
Keyword(s):  
Cytotoxic Effect ◽  
Human Fibroblasts ◽  
Normal Human

Anti-biofilm activity of chitosan gels formulated with silver nanoparticles and their cytotoxic effect on human fibroblasts

2016 ◽  
Vol 60 ◽  
pp. 317-323 ◽  
Author(s):  
M. Pérez-Díaz ◽  
E. Alvarado-Gomez ◽  
M. Magaña-Aquino ◽  
R. Sánchez-Sánchez ◽  
C. Velasquillo ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cytotoxic Effect ◽  
Human Fibroblasts

scholarly journals Evaluation of the cytotoxicity of two endodontic cements based on calcium silicate and Pulp Canal Sealer cement in human fibroblasts

2020 ◽  
Vol 7 (12) ◽  
pp. 298-303
Author(s):  
Leonardo Lemos Andrade ◽  
Carlos Eduardo da Silveira Bueno ◽  
Claudia Fernandes de Magalhães Silveira ◽  
Eduardo Fernandes Marques ◽  
Camila Paiva Macedo ◽  
...  
Keyword(s):  
Calcium Silicate ◽  
Human Fibroblasts

scholarly journals Cytotoxicity assessment of Bio-C Repair Íon+: A new calcium silicate-based cement

2021 ◽  
Vol 15 (3) ◽  
pp. 152-156
Author(s):  
Celso Afonso Klein-Junior ◽  
Roberto Zimmer ◽  
Tãnyre Dobler ◽  
Vanessa Oliveira ◽  
Daniel Rodrigo Marinowic ◽  
...  
Keyword(s):  
Cell Viability ◽  
Calcium Silicate ◽  
Metallic Matrix ◽  
Physical And Mechanical Properties ◽  
Mean Values ◽  
Significance Level ◽  
Pulp Capping ◽  
Cytotoxicity Assessment ◽  
And Control ◽  
Nih 3T3

Background. Direct pulp capping is a method designed to preserve the exposed dental pulp. Due to good biological, physical, and mechanical properties, new versions of calcium silicate-based materials have been developed as pulp capping materials. The present study aimed to evaluate the cytotoxic effects of four calcium silicate-based pulp capping materials, of which the Bio-C Repair Íon+ is still in an experimental phase. Methods. Biodentine, MTA Repair HP, Bio-C Repair, and Bio-C Repair Íon+ cements were dispensed in a metallic matrix to produce 125-mm3 specimens, which were immersed in Dulbecco’s Modified Eagle Medium (DMEM) to obtain extracts. NIH 3T3 cells were cultured and exposed to the extracts for 24 hours and seven days. Cell viability was assessed by the methyl tetrazolium test (MTT). The mean values for the experimental and control groups (without treatment) were compared by analysis of variance (ANOVA) and post hoc Tukey tests, considering a significance level of 5%. Results. All the tested materials demonstrated a reduction in cell viability (P<0.05). According to ISO 10993-5: 2009 (E), Bio-C Repair Íon+ exhibited mild and moderate cytotoxicity in the 24- hour and 7-day analyses, respectively. Bio-C Repair and Biodentine showed mild cytotoxicity, and MTA Repair HP exhibited moderate cytotoxicity at both intervals. Conclusion. The highest cell viability was demonstrated by Biodentine, MTA, and Repair HP, in descending order. Bio-C Repair and Bio-C Repair Íon+ showed moderate cytotoxicity, similar to MTA Repair HP in the 7-day analysis.

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