scholarly journals Effects of CO2 laser irradiation on matrix-rich biofilm development formation – An in vitro study

2016 ◽  
Author(s):  
Bruna Raquel Zancope ◽  
Vanessa B Dainezi ◽  
Marinês Nobre-dos-Santos ◽  
Sillas Duarte, Jr ◽  
Vanessa Pardi ◽  
...  
Keyword(s):  
Contact Angle ◽  
Laser Irradiation ◽  
Co2 Laser ◽  
Biofilm Formation ◽  
In Vitro Study ◽  
Vitro Study ◽  
Enamel Surface ◽  
Control Group ◽  
Biofilm Development

Background. CO2 laser has been used to morphologically and chemically modify the dental enamel surface as well as to turn it more resistant to demineralization. Despite a variety of experiments demonstrating the inhibitory effect of CO2 laser in reduce enamel demineralization, little is known about the effect of surface irradiated on bacterial growth. Thus, this in vitro study was preformed to evaluate the biofilm formation on enamel previously irradiated with a CO2 laser (λ = 10.6 µM). Methods. For this in vitro study, it was employed 96 specimens of bovine enamel, which were divided into 2 groups (n = 48): 1) Control-non-irradiated surface and 2) Irradiated enamel surface. Biofilms were grown on the enamel specimens by 1, 3 and 5 days under intermittent cariogenic condition in the irradiated and non irradiated surface. In each assessment time, the biofilm were evaluated by dry weigh, counting the number of viable colonies and in fifth day, were evaluated by polysaccharides analysis, quantitative real time PCR as well as by contact angle. In addition, the morphology of biofilms was characterized by fluorescence microscopy and field emission scanning electron microscopy (FESEM). Initially, the assumptions of equal variances and normal distribution of errors were conferred and the results are analyzed statistically by t-test and Mann Whitney test. Results. The mean of log CFU/ml obtained for the 1-day biofilm evaluation showed that there is statistical difference between the experimental groups. When biofilms were exposed to CO2 laser, CFU/mL and CFU/ Dry Weight in 3 day was reduced significantly compared with control group. The difference in the genes expression (gtfB and gbpB) and polysaccharides was not statically significant. Contact angle was increased relative to control when the surface was irradiated with CO2 laser. Similar morphology was also visible with both treatments, however irradiated group revealed evidence of melting and fusion in the specimens. Conclusion. In conclusion CO2 laser irradiation modify the energy surface and disrupt the initial biofilm formation.

scholarly journals Effects of CO2 laser irradiation on matrix-rich biofilm development formation – An in vitro study

2016 ◽  
Author(s):  
Bruna Raquel Zancope ◽  
Vanessa B Dainezi ◽  
Marinês Nobre-dos-Santos ◽  
Sillas Duarte, Jr ◽  
Vanessa Pardi ◽  
...  
Keyword(s):  
Contact Angle ◽  
Laser Irradiation ◽  
Co2 Laser ◽  
Biofilm Formation ◽  
In Vitro Study ◽  
Vitro Study ◽  
Enamel Surface ◽  
Control Group ◽  
Biofilm Development

Background. CO2 laser has been used to morphologically and chemically modify the dental enamel surface as well as to turn it more resistant to demineralization. Despite a variety of experiments demonstrating the inhibitory effect of CO2 laser in reduce enamel demineralization, little is known about the effect of surface irradiated on bacterial growth. Thus, this in vitro study was preformed to evaluate the biofilm formation on enamel previously irradiated with a CO2 laser (λ = 10.6 µM). Methods. For this in vitro study, it was employed 96 specimens of bovine enamel, which were divided into 2 groups (n = 48): 1) Control-non-irradiated surface and 2) Irradiated enamel surface. Biofilms were grown on the enamel specimens by 1, 3 and 5 days under intermittent cariogenic condition in the irradiated and non irradiated surface. In each assessment time, the biofilm were evaluated by dry weigh, counting the number of viable colonies and in fifth day, were evaluated by polysaccharides analysis, quantitative real time PCR as well as by contact angle. In addition, the morphology of biofilms was characterized by fluorescence microscopy and field emission scanning electron microscopy (FESEM). Initially, the assumptions of equal variances and normal distribution of errors were conferred and the results are analyzed statistically by t-test and Mann Whitney test. Results. The mean of log CFU/ml obtained for the 1-day biofilm evaluation showed that there is statistical difference between the experimental groups. When biofilms were exposed to CO2 laser, CFU/mL and CFU/ Dry Weight in 3 day was reduced significantly compared with control group. The difference in the genes expression (gtfB and gbpB) and polysaccharides was not statically significant. Contact angle was increased relative to control when the surface was irradiated with CO2 laser. Similar morphology was also visible with both treatments, however irradiated group revealed evidence of melting and fusion in the specimens. Conclusion. In conclusion CO2 laser irradiation modify the energy surface and disrupt the initial biofilm formation.

scholarly journals Effects of CO2laser irradiation on matrix-rich biofilm development formation–an in vitro study

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2458 ◽  
Author(s):  
Bruna Raquel Zancopé ◽  
Vanessa B. Dainezi ◽  
Marinês Nobre-dos-Santos ◽  
Sillas Duarte ◽  
Vanessa Pardi ◽  
...  
Keyword(s):  
Contact Angle ◽  
Biofilm Formation ◽  
Dental Enamel ◽  
In Vitro Study ◽  
Dry Weight ◽  
Vitro Study ◽  
Enamel Surface ◽  
Control Group ◽  
Biofilm Development

BackgroundA carbon dioxide (CO2) laser has been used to morphologically and chemically modify the dental enamel surface as well as to make it more resistant to demineralization. Despite a variety of experiments demonstrating the inhibitory effect of a CO2laser in reduce enamel demineralization, little is known about the effect of surface irradiated on bacterial growth. Thus, this in vitro study was preformed to evaluate the biofilm formation on enamel previously irradiated with a CO2laser (λ = 10.6 µM).MethodsFor this in vitro study, 96 specimens of bovine enamel were employed, which were divided into two groups (n = 48): 1) Control-non-irradiated surface and 2) Irradiated enamel surface. Biofilms were grown on the enamel specimens by one, three and five days under intermittent cariogenic condition in the irradiated and non-irradiated surface. In each assessment time, the biofilm were evaluated by dry weigh, counting the number of viable colonies and, in fifth day, were evaluated by polysaccharides analysis, quantitative real time Polymerase Chain Reaction (PCR) as well as by contact angle. In addition, the morphology of biofilms was characterized by fluorescence microscopy and field emission scanning electron microscopy (FESEM). Initially, the assumptions of equal variances and normal distribution of errors were conferred and the results are analyzed statistically by t-test and Mann Whitney test.ResultsThe mean of log CFU/mL obtained for the one-day biofilm evaluation showed that there is statistical difference between the experimental groups. When biofilms were exposed to the CO2laser, CFU/mL and CFU/dry weight in three day was reduced significantly compared with control group. The difference in the genes expression (Glucosyltransferases (gtfB) and Glucan-binding protein (gbpB)) and polysaccharides was not statically significant. Contact angle was increased relative to control when the surface was irradiated with the CO2laser. Similar morphology was also visible with both treatments; however, the irradiated group revealed evidence of melting and fusion in the specimens.ConclusionIn conclusion, CO2laser irradiation modifies the energy surface and disrupts the initial biofilm formation.

Combined Tin-Containing Fluoride Solution and CO2 Laser Treatment Reduces Enamel Erosion in vitro

2015 ◽  
Vol 49 (6) ◽  
pp. 565-574 ◽  
Author(s):  
Marcella Esteves-Oliveira ◽  
Nadine Witulski ◽  
Ralf-Dieter Hilgers ◽  
Christian Apel ◽  
Hendrik Meyer-Lueckel ◽  
...  
Keyword(s):  
Surface Treatment ◽  
Laser Irradiation ◽  
Co2 Laser ◽  
In Vitro Study ◽  
Vitro Study ◽  
Enamel Surface ◽  
Fluoride Solution ◽  
Surface Loss ◽  
Linear Contrasts

The aim of this in vitro study was to evaluate the effect of combined CO2 laser and tin-containing fluoride treatment on the formation and progression of enamel erosive lesions. Ninety-six human enamel samples were obtained, stored in thymol solution and, after surface polishing, randomly divided into 6 different surface treatment groups (n = 16 in each group) as follows: no treatment, control (C); one CO2 laser irradiation (L1); two CO2 laser irradiations (L2); daily application of fluoride solution (F); combined daily fluoride solution + one CO2 laser irradiation (L1F), and combined daily fluoride solution + two CO2 laser irradiations (L2F). Laser irradiation was performed at 0.3 J/cm2 (5 µs/226 Hz/10.6 µm) on day 1 (L1) and day 6 (L2). The fluoride solution contained AmF/NaF (500 ppm F), and SnCl2 (800 ppm Sn) at pH 4.5. After surface treatment the samples were submitted to an erosive cycling over 10 days, including immersion in citric acid (2 min/0.05 M/pH = 2.3) 6 times daily and storage in remineralization solution (≥1 h) between erosive attacks. At the end of each cycling day, the enamel surface loss (micrometers) was measured using a 3D laser profilometer. Data were statistically analyzed by means of a 2-level mixed effects model and linear contrasts (α = 0.05). Group F (-3.3 ± 2.0 µm) showed significantly lower enamel surface loss than groups C (-27.22 ± 4.1 µm), L1 (-18.3 ± 4.4 µm) and L2 (-16.3 ± 5.3 µm) but higher than L1F (-1.0 ± 4.4 µm) and L2F (1.4 ± 3.2 µm, p < 0.05). Under the conditions of this in vitro study, the tin-containing fluoride solution caused 88% reduction of enamel surface loss, while its combination with CO2 laser irradiation at 0.3 J/cm2 hampered erosive loss almost completely.

scholarly journals Effect of CPP-ACP paste with and without CO2 laser irradiation on demineralized enamel microhardness and bracket shear bond strength

2017 ◽  
Vol 22 (4) ◽  
pp. 53-60 ◽  
Author(s):  
Nasrin Farhadian ◽  
Loghman Rezaei-Soufi ◽  
Seyed Farzad Jamalian ◽  
Maryam Farhadian ◽  
Shahrzad Tamasoki ◽  
...  
Keyword(s):  
Bond Strength ◽  
Laser Irradiation ◽  
Co2 Laser ◽  
Shear Bond Strength ◽  
In Vitro Study ◽  
Enamel Surface ◽  
Control Group ◽  
Demineralized Enamel ◽  
Group 2

ABSTRACT Introduction: Many patients seeking orthodontic treatment already have incipient enamel lesions and should be placed under preventive treatments. The aim of this in vitro study was to evaluate the effect of CPP-ACP paste and CO2 laser irradiation on demineralized enamel microhardness and shear bond strength of orthodontic brackets. Methods: Eighty caries-free human premolars were subjected to a demineralization challenge using Streptococcus mutans. After demineralization, the samples were randomly divided into five equal experimental groups: Group 1 (control), the brackets were bonded without any surface treatment; Group 2, the enamel surfaces were treated with CPP-ACP paste for 4 minutes before bonding; Group 3, the teeth were irradiated with CO2 laser beams at a wavelength of 10.6 µm for 20 seconds. The samples in Groups 4 and 5 were treated with CO2 laser either before or through CPP-ACP application. SEM photomicrographs of a tooth from each group were taken to observe the enamel surface. The brackets were bonded to the buccal enamel using a conventional method. Shear bond strength of brackets and ARI scores were measured. Vickers microhardness was measured on the non-bonded enamel surface. Data were analyzed with ANOVA and Tukey test at the p< 0.05 level. Results: The mean shear bond strength and microhardness of the laser group were higher than those in the control group and this difference was statistically significant (p< 0.05). All groups showed a higher percentage of ARI score 4. Conclusion: CO2 laser at a wavelength of 10.6 µm significantly increased demineralized enamel microhardness and enhanced bonding to demineralized enamel.

Effects of CO2 Laser Irradiation on Treatment of Hypersensitive Dental Necks: Results of an in Vitro Study

1995 ◽  
Vol 13 (6) ◽  
pp. 397-400 ◽  
Author(s):  
A. MORITZ ◽  
N. GUTKNECHT ◽  
U. SCHOOP ◽  
J. WERNISCH ◽  
F. LAMPERT ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Co2 Laser ◽  
In Vitro Study ◽  
Vitro Study

Effect of Nd:YAG and CO2 Laser Irradiation on Prevention of Enamel Demineralization in Orthodontics: In Vitro Study

2017 ◽  
Vol 35 (5) ◽  
pp. 282-286 ◽  
Author(s):  
Renato Siva Paulos ◽  
Priscila Yumi Seino ◽  
Karen Akemi Fukushima ◽  
Marcia Martins Marques ◽  
Fernanda Campos Sousa de Almeida ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Co2 Laser ◽  
In Vitro Study ◽  
Vitro Study ◽  
Enamel Demineralization

CO2 laser irradiation enhances CaF2 formation and inhibits lesion progression on demineralized dental enamel—in vitro study

2016 ◽  
Vol 31 (3) ◽  
pp. 539-547 ◽  
Author(s):  
Bruna R. Zancopé ◽  
Lívia P. Rodrigues ◽  
Thais M. Parisotto ◽  
Carolina Steiner-Oliveira ◽  
Lidiany K. A. Rodrigues ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Co2 Laser ◽  
Dental Enamel ◽  
In Vitro Study ◽  
Vitro Study

Intrapulpar temperature during continuous CO2 laser irradiation in human molars: An in vitro study

1997 ◽  
Vol 9 (6) ◽  
pp. 291-294 ◽  
Author(s):  
Walter Niccoli‐Filho ◽  
José Camilo Furlani ◽  
Carlos Schwab ◽  
Fernando Vagner Raldi ◽  
Carlos de Paula Eduardo
Keyword(s):  
Laser Irradiation ◽  
Co2 Laser ◽  
In Vitro Study ◽  
Vitro Study ◽  
Continuous Co2 Laser

scholarly journals Comparative Evaluation of protective Potential of Toothmin and Novamin Containing Toothpastes on Enamel Surface Under Confocal Microscope: An In Vitro Study

2016 ◽  
Vol 04 (02) ◽  
pp. 113-116 ◽  
Author(s):  
Anurag Aggarwal ◽  
Shalu Gupta ◽  
Kanwarpreet Singh ◽  
Simran Bindra
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Enamel Surface ◽  
Control Group ◽  
Protective Potential ◽  
Study Materials ◽  
Prior Application ◽  
And Control ◽  
Sucrose Phosphate

Abstract Aim: To evaluate the protective potential of calcium sucrose phosphate and novamin containing toothpaste on enamel surface. Settings and Design: In vitro-study. Materials and Methods: This study consisted of 30 samples embedded in orthodontic resin with either buccal or lingual surface exposed. The samples were assigned to either calcium sucrose phosphate containing paste; novamine toothpaste; or control group. The groups were then subjected to cycling in a demineralizing solution and a remineralizing solution. Groups II and III received prior application of calcium sucrose phosphate paste and novamine toothpaste respectively followed by cycling in a demineralizing solution and a remineralizing solution. Following 14 days of cycling, the samples were sectioned and examined using confocal microscopy. The depths of lesions were evaluated. Statistical Analysis: Image Proplus software was used to analyze the images. The values were statistically evaluated using one – way ANOVA and Scheffe's Test. Results and Conclusion: Within the limitations of study it was concluded that enamel surfaces treated with calcium sucrose phosphate paste exhibited the least lesion depths followed by enamel surfaces treated with the novamin tooth paste and control group respectively.

scholarly journals Comparison of different final irrigant agitation techniques for the removal of Enterococcus faecalis biofilms from root canals: an in vitro study

2018 ◽  
Vol 21 (4) ◽  
pp. 386
Author(s):  
Gurkan Gunec ◽  
Faruk Haznedaroglu ◽  
Guven Kulekci ◽  
Nursen Bakir Topcuoglu ◽  
Mutlu Özcan
Keyword(s):  
Enterococcus Faecalis ◽  
Resin Composite ◽  
In Vitro Study ◽  
Positive Control ◽  
Vitro Study ◽  
Positive Control Group ◽  
Control Group ◽  
Root Canals ◽  
Biofilm Development

<p><strong>Objective: </strong>The aim of this in vitro study was to compare the effectiveness of different final irrigant agitation techniques in the removal of <em>Enterococcus faecalis</em> biofilms from root canals. <strong>Material and Methods:</strong> In total, the root canals of 85 extracted single-rooted human maxillary incisors teeth were prepared using the Revo-S system to a 40/06 size. The apical foramen of each tooth was sealed by light-cured resin composite material to obstruct bacterial leakage. The specimens were sterilized in an autoclave at 121°C for 15 min and stored until further use. All teeth except five (negative control group) were inoculated with <em>Enterococcus faecalis</em> and incubated in a CO<sub>2</sub> chamber at 37°C for 7 days; the trypticase soy broth was changed every 2 days. For the determination of possible biofilm formation, five of the 80 teeth were randomly selected as a positive control group; one tooth of positive control group was analysed for biofilm development by scanning electron microscope (SEM) and these teeth received no final irrigant agitation procedure. Then, the remaining 75 teeth were randomly divided into five test groups (n=15 each) and were sequentially irrigated with 5% sodium hypochlorite (NaOCl), 17% ethylenediaminetetraacetic acid and 5% NaOCl. Following each irrigant application, different final irrigant agitation techniques were introduced for 60 s (3×20-s sessions). Group 1 received manual–dynamic agitation, group 2 received passive ultrasonic agitation (PUI), group 3 received EndoActivator agitation, group 4 received photon-initiated photoacoustic streaming (PIPS) with the Er:YAG laser and group 5 received conventional syringe irrigation. Colony-forming units (CFUs) were counted in samples from the positive control and test groups. Data were analysed using Kruskal–Wallis and post-hoc Mann–Whitney U multiple comparison tests. <strong>Results:</strong> <em>E. faecalis </em>elimination was significantly better in the experimental groups than in the positive control groups (p &lt; 0.001). Manual–dynamic agitation and conventional syringe irrigation, with no significant differences between the two groups. <strong>Conclusion:</strong> Essentially, CFU reduction was significantly greater in the PUI, EndoActivator and PIPS groups than in the manual–dynamic agitation and conventional syringe irrigation groups (p &lt;0.001), with no significant differences among the former three groups.</p><p><strong>Keywords</strong></p><p>Enterococcus faecalis; Irrigant agitation; Irrigant solutions; Photon-initiated photoacoustic streaming; Passive ultrasonic irrigation.</p>

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