Effects of CO2laser irradiation on matrix-rich biofilm development formation–an in vitro study

BackgroundA carbon dioxide (CO2) laser has been used to morphologically and chemically modify the dental enamel surface as well as to make it more resistant to demineralization. Despite a variety of experiments demonstrating the inhibitory effect of a CO2laser in reduce enamel demineralization, little is known about the effect of surface irradiated on bacterial growth. Thus, this in vitro study was preformed to evaluate the biofilm formation on enamel previously irradiated with a CO2laser (λ = 10.6 µM).MethodsFor this in vitro study, 96 specimens of bovine enamel were employed, which were divided into two groups (n = 48): 1) Control-non-irradiated surface and 2) Irradiated enamel surface. Biofilms were grown on the enamel specimens by one, three and five days under intermittent cariogenic condition in the irradiated and non-irradiated surface. In each assessment time, the biofilm were evaluated by dry weigh, counting the number of viable colonies and, in fifth day, were evaluated by polysaccharides analysis, quantitative real time Polymerase Chain Reaction (PCR) as well as by contact angle. In addition, the morphology of biofilms was characterized by fluorescence microscopy and field emission scanning electron microscopy (FESEM). Initially, the assumptions of equal variances and normal distribution of errors were conferred and the results are analyzed statistically by t-test and Mann Whitney test.ResultsThe mean of log CFU/mL obtained for the one-day biofilm evaluation showed that there is statistical difference between the experimental groups. When biofilms were exposed to the CO2laser, CFU/mL and CFU/dry weight in three day was reduced significantly compared with control group. The difference in the genes expression (Glucosyltransferases (gtfB) and Glucan-binding protein (gbpB)) and polysaccharides was not statically significant. Contact angle was increased relative to control when the surface was irradiated with the CO2laser. Similar morphology was also visible with both treatments; however, the irradiated group revealed evidence of melting and fusion in the specimens.ConclusionIn conclusion, CO2laser irradiation modifies the energy surface and disrupts the initial biofilm formation.

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Effects of CO2 laser irradiation on matrix-rich biofilm development formation – An in vitro study

10.7287/peerj.preprints.2084 ◽

2016 ◽

Author(s):

Bruna Raquel Zancope ◽

Vanessa B Dainezi ◽

Marinês Nobre-dos-Santos ◽

Sillas Duarte, Jr ◽

Vanessa Pardi ◽

...

Keyword(s):

Contact Angle ◽

Laser Irradiation ◽

Co2 Laser ◽

Biofilm Formation ◽

In Vitro Study ◽

Vitro Study ◽

Enamel Surface ◽

Control Group ◽

Biofilm Development

Background. CO2 laser has been used to morphologically and chemically modify the dental enamel surface as well as to turn it more resistant to demineralization. Despite a variety of experiments demonstrating the inhibitory effect of CO2 laser in reduce enamel demineralization, little is known about the effect of surface irradiated on bacterial growth. Thus, this in vitro study was preformed to evaluate the biofilm formation on enamel previously irradiated with a CO2 laser (λ = 10.6 µM). Methods. For this in vitro study, it was employed 96 specimens of bovine enamel, which were divided into 2 groups (n = 48): 1) Control-non-irradiated surface and 2) Irradiated enamel surface. Biofilms were grown on the enamel specimens by 1, 3 and 5 days under intermittent cariogenic condition in the irradiated and non irradiated surface. In each assessment time, the biofilm were evaluated by dry weigh, counting the number of viable colonies and in fifth day, were evaluated by polysaccharides analysis, quantitative real time PCR as well as by contact angle. In addition, the morphology of biofilms was characterized by fluorescence microscopy and field emission scanning electron microscopy (FESEM). Initially, the assumptions of equal variances and normal distribution of errors were conferred and the results are analyzed statistically by t-test and Mann Whitney test. Results. The mean of log CFU/ml obtained for the 1-day biofilm evaluation showed that there is statistical difference between the experimental groups. When biofilms were exposed to CO2 laser, CFU/mL and CFU/ Dry Weight in 3 day was reduced significantly compared with control group. The difference in the genes expression (gtfB and gbpB) and polysaccharides was not statically significant. Contact angle was increased relative to control when the surface was irradiated with CO2 laser. Similar morphology was also visible with both treatments, however irradiated group revealed evidence of melting and fusion in the specimens. Conclusion. In conclusion CO2 laser irradiation modify the energy surface and disrupt the initial biofilm formation.

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Effects of CO2 laser irradiation on matrix-rich biofilm development formation – An in vitro study

10.7287/peerj.preprints.2084v1 ◽

2016 ◽

Author(s):

Bruna Raquel Zancope ◽

Vanessa B Dainezi ◽

Marinês Nobre-dos-Santos ◽

Sillas Duarte, Jr ◽

Vanessa Pardi ◽

...

Keyword(s):

Contact Angle ◽

Laser Irradiation ◽

Co2 Laser ◽

Biofilm Formation ◽

In Vitro Study ◽

Vitro Study ◽

Enamel Surface ◽

Control Group ◽

Biofilm Development

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Comparative Evaluation of protective Potential of Toothmin and Novamin Containing Toothpastes on Enamel Surface Under Confocal Microscope: An In Vitro Study

Dental Journal of Advance Studies ◽

10.1055/s-0038-1672055 ◽

2016 ◽

Vol 04 (02) ◽

pp. 113-116 ◽

Cited By ~ 1

Author(s):

Anurag Aggarwal ◽

Shalu Gupta ◽

Kanwarpreet Singh ◽

Simran Bindra

Keyword(s):

In Vitro Study ◽

Vitro Study ◽

Enamel Surface ◽

Control Group ◽

Protective Potential ◽

Study Materials ◽

Prior Application ◽

And Control ◽

Sucrose Phosphate

Abstract Aim: To evaluate the protective potential of calcium sucrose phosphate and novamin containing toothpaste on enamel surface. Settings and Design: In vitro-study. Materials and Methods: This study consisted of 30 samples embedded in orthodontic resin with either buccal or lingual surface exposed. The samples were assigned to either calcium sucrose phosphate containing paste; novamine toothpaste; or control group. The groups were then subjected to cycling in a demineralizing solution and a remineralizing solution. Groups II and III received prior application of calcium sucrose phosphate paste and novamine toothpaste respectively followed by cycling in a demineralizing solution and a remineralizing solution. Following 14 days of cycling, the samples were sectioned and examined using confocal microscopy. The depths of lesions were evaluated. Statistical Analysis: Image Proplus software was used to analyze the images. The values were statistically evaluated using one – way ANOVA and Scheffe's Test. Results and Conclusion: Within the limitations of study it was concluded that enamel surfaces treated with calcium sucrose phosphate paste exhibited the least lesion depths followed by enamel surfaces treated with the novamin tooth paste and control group respectively.

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Comparison of different final irrigant agitation techniques for the removal of Enterococcus faecalis biofilms from root canals: an in vitro study

Brazilian Dental Science ◽

10.14295/bds.2018.v21i4.1599 ◽

2018 ◽

Vol 21 (4) ◽

pp. 386

Author(s):

Gurkan Gunec ◽

Faruk Haznedaroglu ◽

Guven Kulekci ◽

Nursen Bakir Topcuoglu ◽

Mutlu Özcan

Keyword(s):

Enterococcus Faecalis ◽

Resin Composite ◽

In Vitro Study ◽

Positive Control ◽

Vitro Study ◽

Positive Control Group ◽

Control Group ◽

Root Canals ◽

Biofilm Development

Objective: The aim of this in vitro study was to compare the effectiveness of different final irrigant agitation techniques in the removal of Enterococcus faecalis biofilms from root canals. Material and Methods: In total, the root canals of 85 extracted single-rooted human maxillary incisors teeth were prepared using the Revo-S system to a 40/06 size. The apical foramen of each tooth was sealed by light-cured resin composite material to obstruct bacterial leakage. The specimens were sterilized in an autoclave at 121°C for 15 min and stored until further use. All teeth except five (negative control group) were inoculated with Enterococcus faecalis and incubated in a CO2 chamber at 37°C for 7 days; the trypticase soy broth was changed every 2 days. For the determination of possible biofilm formation, five of the 80 teeth were randomly selected as a positive control group; one tooth of positive control group was analysed for biofilm development by scanning electron microscope (SEM) and these teeth received no final irrigant agitation procedure. Then, the remaining 75 teeth were randomly divided into five test groups (n=15 each) and were sequentially irrigated with 5% sodium hypochlorite (NaOCl), 17% ethylenediaminetetraacetic acid and 5% NaOCl. Following each irrigant application, different final irrigant agitation techniques were introduced for 60 s (3×20-s sessions). Group 1 received manual–dynamic agitation, group 2 received passive ultrasonic agitation (PUI), group 3 received EndoActivator agitation, group 4 received photon-initiated photoacoustic streaming (PIPS) with the Er:YAG laser and group 5 received conventional syringe irrigation. Colony-forming units (CFUs) were counted in samples from the positive control and test groups. Data were analysed using Kruskal–Wallis and post-hoc Mann–Whitney U multiple comparison tests. Results: E. faecalis elimination was significantly better in the experimental groups than in the positive control groups (p < 0.001). Manual–dynamic agitation and conventional syringe irrigation, with no significant differences between the two groups. Conclusion: Essentially, CFU reduction was significantly greater in the PUI, EndoActivator and PIPS groups than in the manual–dynamic agitation and conventional syringe irrigation groups (p <0.001), with no significant differences among the former three groups.KeywordsEnterococcus faecalis; Irrigant agitation; Irrigant solutions; Photon-initiated photoacoustic streaming; Passive ultrasonic irrigation.

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Influence of Citric Acid Concentration and Etching Time on Enamel Surface Roughness of Prepared Human Tooth: in vitro Study

Plasticity, Damage and Fracture in Advanced Materials - Advanced Structured Materials ◽

10.1007/978-3-030-34851-9_8 ◽

2019 ◽

pp. 135-150

Author(s):

Evgeniy V. Sadyrin ◽

Evgeniy A. Kislyakov ◽

Roman V. Karotkiyan ◽

Diana V. Yogina ◽

Ekaterina G. Drogan ◽

...

Keyword(s):

Surface Roughness ◽

Citric Acid ◽

Acid Concentration ◽

In Vitro Study ◽

Vitro Study ◽

Enamel Surface ◽

Human Tooth ◽

Etching Time ◽

Citric Acid Concentration

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Effect of topical fluoride on microshear bond strength of primary enamel to composite, microhardness of enamel and its surface morphology: An in vitro study

Journal of Dental Research Dental Clinics Dental Prospects ◽

10.15171/joddd.2019.046 ◽

2019 ◽

Vol 13 (4) ◽

pp. 305-310

Author(s):

Mina Biria ◽

Sajedeh Namaei Ghasemi ◽

Seyedeh Mahsa Sheikh-Al-Eslamian ◽

Narges Panahandeh

Keyword(s):

Bond Strength ◽

Artificial Saliva ◽

Morphological Characteristics ◽

In Vitro Study ◽

Vitro Study ◽

Control Group ◽

Modes Of Failure ◽

Group 3 ◽

Microshear Bond Strength

Background. This in vitro study aimed to evaluate the microshear bond strength (μSBS), microhardness and morphological characteristics of primary enamel after treating with sodium fluoride (NaF) and acidulated phosphate fluoride (APF). Methods. Forty-eight primary canines were cut into mesial and distal sections and assigned to five groups randomly: group 1 (immersed in saliva as a control), group 2 (treated with NAF and immersed in saliva for 30 minutes), group 3 (treated with APF and immersed in saliva for 30 minutes), group 4 (treated with NAF and immersed in saliva for 10 days), and group 5 (treated with APF and immersed in saliva for 10 days). Composite resin (Filtek Z250) was bonded on the specimens (n=15) for measuring the μSBS. After storage in 37°C artificial saliva for 24 hours, µSBS and Vickers hardness tests (10 readings) were performed. The data were analyzed using one-way ANOVA and Kolmogorov-Smirnov, Levene’s and Tukey HSD tests (P<0.05). Morphological analysis of enamel and modes of failure were carried out under a scanning electron microscope (SEM) on two remaining specimens. Results. Significant differences in μSBS were only noted between groups 2 and 4 (P=0.024). Group 3 showed a significant decrease in hardness after storage in artificial saliva (P<0.001), with a significantly lower hardness than the other groups (P<0.001). The SEM observations showed irregular particles in groups 3 and 5; uniform, smooth and thin coats were seen in groups 2 and 4. Conclusion. Fluoride therapy with NaF and APF gels prior to restorative treatments had no adverse effects on the microshear bond strength.

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