scholarly journals Effect of temperature on growth of Pseudomonas protegens Pf-5 and Pseudomonas aeruginosa PRD-10 in LB Lennox medium

2018 ◽  
Author(s):  
Wenfa Ng
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Optical Density ◽  
The Other ◽  
Temperature Adaptation ◽  
Effect Of Temperature ◽  
Metabolic Processes ◽  
Two Phase ◽  
Ph Variation ◽  
Temperature Range ◽  
Other Hand

Temperature affects growth of bacteria by influencing enzyme and growth kinetics. Specifically, evolution selects for specific temperature range in which a microbe could thrive, and thus fix the temperature range in which biomolecule structure and function are finely tuned for coping with the thermal conditions prevailing within a cell at a particular temperature. Using aerobic culture in LB Lennox medium in shake flasks, this study aimed to understand the growth of Pseudomonas protegens Pf-5 (ATCC BAA-477) and Pseudomonas aeruginosa PRD-10 (ATCC 15442) at 25, 30 and 37 oC. Experiment results revealed that P. protegens Pf-5 grew very poorly at 37 oC (with maximal optical density of 0.66), while better growth was observed at 25 and 30 oC. Specifically, P. protegens Pf-5 appeared to be better adapted to growth at 25 oC, where the maximal optical density obtained was 5.3 compared to 4.6 at 30 oC. More importantly, two phase growth behaviour was observed during growth at 30 oC where a faster initial phase of growth was followed by a slower one. Growth at 25 and 30 oC exhibited similar pH trend, which suggested similar metabolic processes was activated during growth. On the other hand, P. aeruginosa PRD-10 demonstrated a more efficient conversion of LB Lennox medium into biomass where the maximal optical density obtained at all three growth temperatures were higher than those of P. protegens Pf-5. More importantly, growth of P. aeruginosa PRD-10 exhibited a clear adaptation to growth at 25 and 37 oC, while growth at 30 oC resulted in a lower biomass yield compared to that of 25 and 37 oC. On the other hand, pH variation during culture revealed that P. aeruginosa PRD-10 likely activated similar metabolic processes at all three growth temperatures, where a higher growth temperature would result in the net secretion of more alkaline metabolites. Collectively, P. protegens Pf-5 and P. aeruginosa PRD-10 demonstrated clear temperature adaptation at an evolutionary level. In addition, experiment data suggested that P. aeruginosa PRD-10 might have co-evolved with humans on a substantial time scale resulting in a temperature preference of 37 oC over 30 oC.

scholarly journals Effect of temperature on growth of Pseudomonas protegens Pf-5 and Pseudomonas aeruginosa PRD-10 in LB Lennox medium

2018 ◽  
Author(s):  
Wenfa Ng
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Optical Density ◽  
The Other ◽  
Temperature Adaptation ◽  
Effect Of Temperature ◽  
Metabolic Processes ◽  
Two Phase ◽  
Ph Variation ◽  
Temperature Range ◽  
Other Hand

Temperature affects growth of bacteria by influencing enzyme and growth kinetics. Specifically, evolution selects for specific temperature range in which a microbe could thrive, and thus fix the temperature range in which biomolecule structure and function are finely tuned for coping with the thermal conditions prevailing within a cell at a particular temperature. Using aerobic culture in LB Lennox medium in shake flasks, this study aimed to understand the growth of Pseudomonas protegens Pf-5 (ATCC BAA-477) and Pseudomonas aeruginosa PRD-10 (ATCC 15442) at 25, 30 and 37 oC. Experiment results revealed that P. protegens Pf-5 grew very poorly at 37 oC (with maximal optical density of 0.66), while better growth was observed at 25 and 30 oC. Specifically, P. protegens Pf-5 appeared to be better adapted to growth at 25 oC, where the maximal optical density obtained was 5.3 compared to 4.6 at 30 oC. More importantly, two phase growth behaviour was observed during growth at 30 oC where a faster initial phase of growth was followed by a slower one. Growth at 25 and 30 oC exhibited similar pH trend, which suggested similar metabolic processes was activated during growth. On the other hand, P. aeruginosa PRD-10 demonstrated a more efficient conversion of LB Lennox medium into biomass where the maximal optical density obtained at all three growth temperatures were higher than those of P. protegens Pf-5. More importantly, growth of P. aeruginosa PRD-10 exhibited a clear adaptation to growth at 25 and 37 oC, while growth at 30 oC resulted in a lower biomass yield compared to that of 25 and 37 oC. On the other hand, pH variation during culture revealed that P. aeruginosa PRD-10 likely activated similar metabolic processes at all three growth temperatures, where a higher growth temperature would result in the net secretion of more alkaline metabolites. Collectively, P. protegens Pf-5 and P. aeruginosa PRD-10 demonstrated clear temperature adaptation at an evolutionary level. In addition, experiment data suggested that P. aeruginosa PRD-10 might have co-evolved with humans on a substantial time scale resulting in a temperature preference of 37 oC over 30 oC.

Mitochondrial membrane fluorescence and temperature adaptation in Schistocephalus solidus (Cestoda: Pseudophyllidea)

Parasitology ◽  
1985 ◽  
Vol 90 (1) ◽  
pp. 131-135 ◽  
Author(s):  
R. W. Walker ◽  
J. Barrett
Keyword(s):  
Gasterosteus Aculeatus ◽  
Arrhenius Plot ◽  
Gallus Domesticus ◽  
Temperature Adaptation ◽  
Effect Of Temperature ◽  
Acclimation Temperature ◽  
Membrane Composition ◽  
Mitochondrial Membranes ◽  
Temperature Range ◽  
Schistocephalus Solidus

The fluorescent probe 1-anilino-8-naphthalene suiphonic acid (ANS) was used to investigate the effect of temperature on the physical state of the mitochondrial membranes of adult and larval schistocephalus solzdus together with that of their hosts Gasterosteus aculeatus and Gallus domesticus. Arrhenius plots of ANS/membrane fluorescence for S. solidus plerocercoids was linear over the temperature range 15 to 58 °C, while that for the adult was biphasic with a discontinuity at 39·9 °C. This was interpreted as a physical change which occurred in the adult membrane but not in the plerocercoid membrane and pointed to an alteration in membrane composition during infection. Gasierosteus aculeatus showed a linear Arrhenius plot for membrane fluorescence, irrespective of acclimation temperature. Gallus domesticus showed a discontinuity in the Arrhenius plot for membrane fluorescence at 46·9 °C, outside the normal physiological temperature range.

Reduction of binary sulfate mixtures containing CuSO4 by H2

1976 ◽  
Vol 54 (23) ◽  
pp. 3651-3657 ◽  
Author(s):  
Fathi Habashi ◽  
Shaheer A. Mikhail
Keyword(s):  
Intermediate Product ◽  
The Other ◽  
Temperature Range ◽  
Other Hand ◽  
First Case ◽  
Limited Temperature ◽  
Limited Temperature Range ◽  
The Individual

The reduction of a binary sulfate mixture cannot be predicted from the behavior of the individual components. Thus, while CuSO4 is reduced to Cu at 400 °C and NiSO4 is reduced to Ni3S2, the sulfate mixture yields Cu, Ni3S2, and Cu2S. Also while FeSO4 is completely stable in H2 at 400 °C, (Cu,Fe) SO4 yields Cu and Cu5FeS4. The formation of Cu2S in the first case and Cu5FeS4 in the second was unexpected. On the other hand, (Cu,Mn)SO4 is stable in H2 up to 550 °C although pure CuSO4 is completely reduced at 400 °C. CoSO4 also interferes when reduced in presence of CuSO4, while Na2SO4, MgSO4, Al2(SO4)3, ZnSO4, CdSO4 do not interfere within limited temperature range. Of these only Na2SO4 forms a complex sulfate with CuSO4. No Cu2SO4 was identified when CuSO4 was reduced in presence of other sulfates although it is an intermediate product during the reduction of pure CuSO4.

FERROMAGNETIC TDAE-C60 VERSUS PARAMAGNETIC TDAE-C70: FARADEY BALANCE AND ESR STUDY

1992 ◽  
Vol 06 (23n24) ◽  
pp. 3953-3958 ◽  
Author(s):  
Kazuyoshi Tanaka ◽  
Anvar A. Zakhidov ◽  
Kazunari Yoshizawa ◽  
Kenji Okahara ◽  
Tokio Yamabe ◽  
...  
Keyword(s):  
Magnetic Properties ◽  
Electron Spin Resonance ◽  
Electron Spin ◽  
Fullerene Molecule ◽  
The Other ◽  
Temperature Range ◽  
Type Transition ◽  
Other Hand ◽  
Spin Resonance ◽  
Ordered State

Magnetic properties of C 60 and C 70 complexes with tetrakis(dimethylamino)ethylene (TDAE) have been studied based on the measurements of the Faraday-balance magnetization and the electron spin resonance (ESR). It has been found that both TDAE- C 60 and TDAE- C 70 possess about one radical spin per fullerene molecule. TDAE- C 60 has been confirmed to show ferromagnetic-type transition at Tc=16.7–17.5 K . Appearance of a new broad and intense ESR signal below Tc of TDAE- C 60 suggests that the radical spins in the magnetically ordered state rather localize on each C 60 molecule and are ferromagnetically correlated. On the other hand, TDAE- C 70 does not show ferromagnetism in all the temperature range down to 4.5K.

Carboxy Nitroso Rubber, Chemistry, Properties, and Applications

1971 ◽  
Vol 44 (1) ◽  
pp. 40-51 ◽  
Author(s):  
N. B. Levine
Keyword(s):  
The Other ◽  
Temperature Range ◽  
Other Hand

Abstract The nitroso copolymer shows excellent promise which has not been realized due to a lack of ability to achieve a suitable cure. The CNR terpolymer, on the other hand, retains the excellent solvent and oxidizer resistance, nonflammability, and wide useful temperature range of the copolymer and can, in addition, be easily fabricated and cured by several techniques into many useful products.

Differential Enthalpies of Solution of Components in Binary Systems 2 CaO . Al2O3 . SiO2-CaO . Al2O3 . 2 SiO2, CaO . SiO2-CaO . Al2O3 . 2 SiO2 and CaO . SiO2-2 CaO . Al2O3 . SiO2

1993 ◽  
Vol 58 (9) ◽  
pp. 1989-1996 ◽  
Author(s):  
Ladislav Kosa ◽  
Ivan Nerád ◽  
Jozef Strečko ◽  
Ivo Proks ◽  
Katarína Adamkovičová
Keyword(s):  
Binary Systems ◽  
The Other ◽  
Enthalpies Of Solution ◽  
Heat Of Solution ◽  
Differential Heat ◽  
Temperature Range ◽  
Enthalpies Of Mixing ◽  
Other Hand ◽  
Temperature Dependences

Differential enthalpies of solution of components in binary systems 2 CaO . Al2O3 . SiO2-CaO . Al2O3 . 2 SiO2, CaO . SiO2-CaO . Al2O3 . 2 SiO2 and CaO . SiO2-2 CaO . Al2O3 . SiO2 as the function of composition and temperature were determined on the base of isothermal composition dependences of enthalpies of mixing and temperature dependences of heats of fusion of their pure components. From the values of the first differential heat of solution of CaO . Al2O3 . 2 SiO2 and 2 CaO . Al2O3 . SiO2 in CaO . SiO2 over temperature range considered we can conclude that the reactions were closed chains of SiO4 tetrahedra in CaO . SiO2 melt break, are exothermic. On the other hand positive values of this quantity for CaO . SiO2 in CaO . Al2O3 . 2 SiO2 and 2 CaO . Al2O3 . SiO2 led us to the conclusion that the progressive breaking originally closed chains in CaO . SiO2 melt has endothermic character.

Exact Solution for Freezing in Cylindrical Symmetry with Extended Freezing Temperature Range

1979 ◽  
Vol 101 (2) ◽  
pp. 331-334 ◽  
Author(s):  
M. N. O¨zis¸ik ◽  
J. C. Uzzell
Keyword(s):  
Solid Solution ◽  
Solid Fraction ◽  
Freezing Temperature ◽  
Cylindrical Symmetry ◽  
Infinite Medium ◽  
The Other ◽  
Phase Zone ◽  
Two Phase ◽  
Temperature Range ◽  
Liquidus Temperatures

The problem of solidification by a line heat sink in an infinite medium with cylindrical symmetry for a substance having an extended freezing temperature range between the solidus and liquidus temperatures is solved exactly for two different cases characterizing the distribution of the solid fraction within the two-phase zone. In one of the models, the solid fraction is assumed to vary linearly with the temperature and in the other solidification within the two-phase zone is assumed to have a linear relationship with the distance. The analysis is applicable for both eutectic and solid solution alloys.

EFFECT OF ANTIBACTERIAL AGENTS ON THE AUTOPLAQUE PHENOMENON OF PSEUDOMONAS AERUGINOSA

1965 ◽  
Vol 11 (2) ◽  
pp. 213-219 ◽  
Author(s):  
Richard S. Berk
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Agents ◽  
Agar Medium ◽  
The Other ◽  
Other Hand ◽  
Low Concentrations ◽  
Glucose Agar

A survey of 75 cultures of Pseudomonas aeruginosa containing only one discernible phenotype indicated that 68 routinely exhibited spontaneous lysis, resulting in the appearance of turbid erosions or autoplaques (AP+) when grown on a tryptone–glucose agar medium. Single supplements of non-inhibitory levels of chloromycetin, aureomycin, tetracycline, declomycin, and streptomycin increased the degree of lysis of AP+ strains, but were without effect on AP− strains. Enhancement of lysis was also obtained with sulfadiazine, sulfathiazole, sulfamethoxypyridazine, elkosin, gantrisin, and triple sulfa, while little or no effect was observed with sulfamerizine and thiosulfil. On the other hand, low concentrations of either acriflavine or proflavine retarded the rate and degree of lysis, while single supplements of the active antibiotics overcame the suppressive effects of the dyes.

Interactions of LO Phonons with Bound Excitons in Homoepitaxial GaN

1997 ◽  
Vol 482 ◽  
Author(s):  
K. P. Koron ◽  
A. Wysmolek ◽  
J. M. Baranowskil ◽  
K. Pakul ◽  
J. P. Bergman ◽  
...  
Keyword(s):  
Temperature Dependence ◽  
The Other ◽  
Direct Influence ◽  
Neutral Donor ◽  
Phonon Replica ◽  
Temperature Range ◽  
Other Hand ◽  
Order Of Magnitude ◽  
Neutral Acceptor

AbstractPhotoluminescence connected with excitons and their phonon replicas in undoped homoepitaxial MOCVD grown GaN layers have been studied in the temperature range 2 - 100 K. It is shown that the coupling between LO phonons and neutral acceptor bound excitons (ABE) is much stronger than the coupling between LO phonons and neutral donor bound excitons (DBE). In spite that emission due the DBE no-phonon is one order of magnitude stronger than the ABE one, the predominant feature of the first LO phonon replica of the excitonic structures is related to the ABE. It is argued that this fact is connected with delocalization of the acceptor wavefunction in the k-space which leads to a higher number of interacting LO phonons in the first replica. On the other hand, the second LO phonon replica of the excitonic structures is predominantly connected with the DBE. In the case of two LO phonons interacting with bound excitons the k - conservation has not so direct influence. In addition, the temperature dependence of LO phonon replicas and their kinetics in ps regime are also reported.

FACTORS AND PROCESSES IN THE GERMINATION OF DATURA FEROX L.

1964 ◽  
Vol 42 (9) ◽  
pp. 1189-1203 ◽  
Author(s):  
A. Soriano ◽  
R. A. Sánchez ◽  
B. A. de Eilberg
Keyword(s):  
High Temperature ◽  
Low Temperature ◽  
Oxidation Product ◽  
Red Light ◽  
Storage Conditions ◽  
The Other ◽  
Metabolic Processes ◽  
Other Hand ◽  
Inhibitor Level

The germination of Datura ferox seeds is blocked immediately after ripeness by an inhibitor. The decoating of the seeds and certain storage conditions promote germination by favoring the diffusion of the inhibitor or its destruction.Seeds in which the germination blockage due to the inhibitor had been overcome behave as photoblastically positive, responding to the R–FR mechanism. During afterripening, the capacity of the skotomechanism increases as the inhibitor level decreases. The skotomechanism blockage does not disappear with low temperature or with alternating temperatures. On the other hand, high temperature reimposes this blockage.Gibberellic acid replaces the effect of red light, whereas thiourea is not effective.It is postulated, on the basis of the results obtained with this species, and the results of other authors, that the quantity of an oxidation product present, resulting from metabolic processes during storage or the action of red light, controls both germination and the possibility of germination being produced in conditions of light or darkness.

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