scholarly journals Cryopreservation of Limnoperna fortunei (golden mussel) sperm with polyethylene glycol

2018 ◽  
Author(s):  
Milica Markovic ◽  
Juliana Alves Americo ◽  
Inês Julia Ribas Wajsenzon ◽  
Yasmin Rodrigues da Cunha ◽  
Thaísa Vieira Santos de Souza ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Survival Rate ◽  
Ethylene Glycol ◽  
Limnoperna Fortunei ◽  
Polyethylene Glycol 4000 ◽  
Cryoprotective Agents ◽  
Viable Sperm ◽  
Golden Mussel ◽  
And Storage ◽  
Cryopreservation Protocol

Background. Insufficient quantities of freshly harvested Limnoperna fortunei gametes and embryos constrain reproduction research in the laboratory. Cryopreservation would allow the accumulation and storage of gametes when they are available. The lack of available cryopreservation protocol for Limnoperna fortunei in the literature led our research group to undertake a study to establish which cryoprotective agents can be might be most useful for cryopreservation of this species’ sperm. Methods. 2%, 5%, and 10% concentration of ethylene glycol, dimethyl sulfoxide, glycerol, and polyethylene glycol 4000 as well as 0.2 M glucose, sucrose, and trehalose (mixed into the 10% concentration of the aforementioned agents) were tested in a 1:1 ratio with sperm, in 0.25 ml straws, frozen in liquid nitrogen. Results. After 48 hours the best survival rate was in the samples with 10% polyethylene glycol 4000, 36.1%, which also resulted in viable sperm after 7 and 15 days.

scholarly journals Cryopreservation of Limnoperna fortunei (golden mussel) sperm with polyethylene glycol

2018 ◽  
Author(s):  
Milica Markovic ◽  
Juliana Alves Americo ◽  
Inês Julia Ribas Wajsenzon ◽  
Yasmin Rodrigues da Cunha ◽  
Thaísa Vieira Santos de Souza ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Survival Rate ◽  
Ethylene Glycol ◽  
Limnoperna Fortunei ◽  
Polyethylene Glycol 4000 ◽  
Cryoprotective Agents ◽  
Viable Sperm ◽  
Golden Mussel ◽  
And Storage ◽  
Cryopreservation Protocol

Background. Insufficient quantities of freshly harvested Limnoperna fortunei gametes and embryos constrain reproduction research in the laboratory. Cryopreservation would allow the accumulation and storage of gametes when they are available. The lack of available cryopreservation protocol for Limnoperna fortunei in the literature led our research group to undertake a study to establish which cryoprotective agents can be might be most useful for cryopreservation of this species’ sperm. Methods. 2%, 5%, and 10% concentration of ethylene glycol, dimethyl sulfoxide, glycerol, and polyethylene glycol 4000 as well as 0.2 M glucose, sucrose, and trehalose (mixed into the 10% concentration of the aforementioned agents) were tested in a 1:1 ratio with sperm, in 0.25 ml straws, frozen in liquid nitrogen. Results. After 48 hours the best survival rate was in the samples with 10% polyethylene glycol 4000, 36.1%, which also resulted in viable sperm after 7 and 15 days.

scholarly journals Cryopreservation of Limnoperna fortunei (golden mussel) sperm with polyethylene glycol

2018 ◽  
Author(s):  
Milica Markovic ◽  
Juliana Alves Americo ◽  
Inês Julia Ribas Wajsenzon ◽  
Yasmin Rodrigues da Cunha ◽  
Thaísa Vieira Santos de Souza ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Survival Rate ◽  
Ethylene Glycol ◽  
Limnoperna Fortunei ◽  
Polyethylene Glycol 4000 ◽  
Cryoprotective Agents ◽  
Viable Sperm ◽  
Golden Mussel ◽  
And Storage ◽  
Cryopreservation Protocol

Background. Insufficient quantities of freshly harvested Limnoperna fortunei gametes and embryos constrain reproduction research in the laboratory. Cryopreservation would allow the accumulation and storage of gametes when they are available. The lack of available cryopreservation protocol for Limnoperna fortunei in the literature led our research group to undertake a study to establish which cryoprotective agents can be might be most useful for cryopreservation of this species’ sperm. Methods. 2%, 5%, and 10% concentration of ethylene glycol, dimethyl sulfoxide, glycerol, and polyethylene glycol 4000 as well as 0.2 M glucose, sucrose, and trehalose (mixed into the 10% concentration of the aforementioned agents) were tested in a 1:1 ratio with sperm, in 0.25 ml straws, frozen in liquid nitrogen. Results. After 48 hours the best survival rate was in the samples with 10% polyethylene glycol 4000, 36.1%, which also resulted in viable sperm after 7 and 15 days.

83 VITRIFICATION OF IMMATURE AND IN VITRO-MATURED HORSE OOCYTES

2006 ◽  
Vol 18 (2) ◽  
pp. 149 ◽  
Author(s):  
L. Bogliolo ◽  
F. Ariu ◽  
I. Rosati ◽  
M. T. Zedda ◽  
S. Pau ◽  
...  
Keyword(s):  
Survival Rate ◽  
Ethylene Glycol ◽  
Survival Rates ◽  
Germinal Vesicle ◽  
Cumulus Cells ◽  
Hoechst 33342 ◽  
Nuclear Maturation ◽  
And Control ◽  
Cryopreservation Protocol

Few attempts have been carried out to cryopreserve equine oocytes, and an effective cryopreservation protocol is not defined yet. Studies were conducted to compare the viability of immature and in vitro-matured horse oocytes vitrified by the minimal volume cooling (MVC) cryotop vitrification method (Kuwayama et al. 2005 Reprod. BioMed. Online 11, 300–308). Oocytes were recovered from slaughterhouse ovaries and divided, on the basis of the morphology of cumulus cells, into cumulus-expanded (CE) and cumulus-compacted (CC) oocytes. Groups of CC and CE oocytes were vitrified immediately after recovery [germinal vesicle (GV) stage] or matured in vitro (IVM) and cryopreserved at the MII stage as follows: oocytes were incubated 30 min in TCM-199 + 20% FCS + 10% ethylene glycol (EG) + 10% DMSO, followed by 20 min in TCM-199 + 20% FCS + 20% EG + 20% DMSO + 0.25 M sucrose, loaded in cryotops (2 µL), and plunged into liquid nitrogen. Warming was performed at 38.5°C by washing the oocytes in TCM-199 + 20% FCS with decreasing sucrose concentrations (1.25 M, 0.62 M, 0.31 M). After warming oocytes cryopreserved at the GV stage were matured in vitro for 24 h (CE) or 36 h (CC) in TCM-199 + 10% FCS + FSH, LH each at (0.1 UI/mL) + cysteamine, fixed, and stained with glycerol-Hoechst 33342 to assess nuclear maturation. Oocytes vitrified at the MII stage were in vitro cultured for 2 h to evaluate their morphological survival on the basis of the presence of an intact zona pellucida and membrane. Nonvitrified oocytes undergoing the same maturation protocol were used as controls. Results (Table 1) indicated that the survival rate of oocytes vitrified at the GV stage, after IVM, was similar between CE and CC oocytes (43.6% vs 42.6%). Significantly (P < 0.01) higher numbers of vitrified CE MII oocytes (52.9%) survived, compared to CC (34.8%), after 2-h culture. The percentages of viable MII oocytes from CE and CC GV vitrified oocytes were 43.6% and 40.9% respectively and were comparable to those from vitrified MII oocytes (CE, 52.9%; CC, 34.8%) and control oocytes (CE, 56.4%; CC, 53.3%). In conclusion, the results of this study showed that vitrification by the MCV Cryotop method of horse oocytes at either the GV or the MII stage allows a similar number of viable mature oocytes to be recovered. Table 1. Maturation and survival rates of immature and mature equine oocytes vitrified by the MCV Cryotop method

Comparison of two different methods for the vitrification of hatched blastocysts from the dromedary camel (Camelus dromedarius)

2005 ◽  
Vol 17 (5) ◽  
pp. 523 ◽  
Author(s):  
J. A. Skidmore ◽  
M. Billah ◽  
N. M. Loskutoff
Keyword(s):  
Polyethylene Glycol ◽  
Survival Rate ◽  
Ethylene Glycol ◽  
Liquid Nitrogen ◽  
Survival Rates ◽  
Camelus Dromedarius ◽  
Dromedary Camel ◽  
Vitrification Solution

The uteri of 32 donor camels were flushed non-surgically on Day 6, 7 or 8 after ovulation and a total of 184 embryos was recovered. Sixty Day 6 embryos and 61 Day 7 embryos were vitrified or frozen ultrarapidly using open pulled straws and a modified version of the Vajta protocol. These embryos were subjected to concentrations of either 10% and 20% or 20% and 40% ethanediol as the cryoprotectant before being loaded into open pulled straws (OPS) and plunged into liquid nitrogen. All embryos were subsequently thawed and rehydrated either directly into holding media or into holding media containing 0.2 m sucrose and were incubated for 5 or 10 min before being transferred to holding media before transfer to recipients. Although the survival rate of the embryos immediately after thawing was high (OPS 20%/40% ethanediol resulted in 97% and 100% survival for Day 6 and Day 7 embryos, respectively; OPS 10%/20% ethanediol resulted in 90% and 70% survival for Day 6 and Day 7 embryos, respectively), after 2 h in culture, survival rates had decreased to 46% and 53% for Day 6 and Day 7 embryos, respectively, using OPS 10%/20% and 53% and 63% for Day 6 and Day 7 embryos, respectively, using OPS 20%/40%; however, none of the embryos transferred resulted in a viable fetus. A further 63 embryos (Day 6: n = 31; Day 7: n = 16; Day 8: n = 16) were subsequently exposed to vitrification solution (20% glycerol + 20% ethylene glycol + 0.3 m sucrose + 0.375 m glucose + 3% polyethylene glycol) in three steps and after loading into 0.25 mL straws were plunged into liquid nitrogen. However, a much greater percentage of the Day 7 and Day 8 embryos (43.8% and 81.2% respectively) were fractured or torn after warming and none of the 12 intact embryos transferred resulted in a pregnancy. Better survival rates immediately after thawing and rehydration were obtained with the smaller Day 6 embryos (94%), which resulted in a total of eight fetuses from the 21 embryos transferred.

scholarly journals Cryopreservation of in vitro-grown shoot tips of chrysanthemum by encapsulation-dehydration

2013 ◽  
Vol 25 (2) ◽  
pp. 133-140 ◽  
Author(s):  
Małgorzata Zalewska ◽  
Dariusz Kulus
Keyword(s):  
Survival Rate ◽  
Sucrose Concentration ◽  
Multiple Shoots ◽  
Shoot Tips ◽  
Long Term Storage ◽  
The Media ◽  
And Storage ◽  
Cryopreservation Protocol

ABSTRACT Chrysanthemums are amongst the most economically important flowers in the world. The protection and storage of these valuable genetic resources is of great importance. Today, cryopreservation, or the storage of biological material at the temperature of liquid nitrogen (-196°C), is believed to be the most promising long-term storage method. To optimise the cryopreservation protocol, the shoot tips of Chrysanthemum × grandiflorum /Ramat./ Kitam. ‘Lady Orange’ and ‘Lady Salmon’ mutants were cryopreserved using the encapsulation-dehydration technique. During the experiment, the influence of sucrose concentration (2, 3 and 6%) during preculture and the concentration of kinetin (0.25, 0.5, 0.75 and 1.0 mg dm-3) in the regrowth medium were tested. A higher survival rate was observed for ‘Lady Salmon’. In general, the media with higher sucrose levels provided the best survival and recovery rates (35-40%). Kinetin had no influence on the survival rate; however, it influenced the morphogenesis of the plants. The lowest number of explants forming multiple shoots was observed on the medium with the lowest sucrose (during preculture) and kinetin (in the recovery medium) concentration. On the other hand, the best rhizogenesis efficiency was observed when 0.25 mg dm-3 kinetin was added. In conclusion, the composition of both preculture and recovery media need to be adjusted to single cultivars. The use of 3% sucrose (preculture) and 0.25 mg dm-3 kinetin (recovery) seems reasonable, since it guarantees a satisfying recovery rate of the explants and at the same time prevents the formation of callus and multiple shoots, stimulating the rooting instead.

Polyethylene glycol/modified carbon foam composites for efficient light-thermal conversion and storage

Polymer ◽  
2021 ◽  
pp. 123894
Author(s):  
Fankai Lin ◽  
Xiaoguang Zhang ◽  
Xianjie Liu ◽  
Yunfei Xu ◽  
Zhenhua Sun ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Carbon Foam ◽  
Thermal Conversion ◽  
And Storage

scholarly journals Toxicity of neem's oil, a potential biocide against the invasive mussel Limnoperna fortunei (Dunker 1857)

2012 ◽  
Vol 84 (4) ◽  
pp. 1065-1071 ◽  
Author(s):  
Patricio J. Pereyra ◽  
Gustavo B. Rossini ◽  
Gustavo Darrigran
Keyword(s):  
Invasive Species ◽  
Toxicity Tests ◽  
Limnoperna Fortunei ◽  
Target Species ◽  
Potential Toxicity ◽  
A Value ◽  
Closed Systems ◽  
The One ◽  
Invasive Mussel ◽  
Golden Mussel

The golden mussel Limnoperna fortunei (Dunker 1857) is one of the most distributed Nuisance Invasive Species (NIS) in South America, and a threat of great concern for the industry of the area. In this study, we carried out toxicity tests made with a Neem's oil solution with L. fortunei larvae and benthonic adults (7, 13 and 19 ± 1 mm). Tests with non-target species (Daphnia magna, Lactuca sativa and Cnesterodon decemmculatus) were also made with the aim to evaluate the potential toxicity of the Neem's solution in the environment. The LC100 of Neem's solution obtained for larvae was 500 µl/L, a value much higher than the one obtained for D. magna and C. decemmaculatus. Thus, we recommend that it should not be used in open waters. However, since the adults were killed in 72 h and the larvae in 24 h, this product can be used in closed systems, in man-made facilities.

scholarly journals Density and body size of the larval stages of the invasive golden mussel (Limnoperna fortunei) in two neotropical rivers

2012 ◽  
Vol 23 (3) ◽  
pp. 282-292 ◽  
Author(s):  
Vivianne Eilers ◽  
Márcia Divina de Oliveira ◽  
Kennedy Francis Roche
Keyword(s):  
Body Size ◽  
Larval Stage ◽  
Larval Density ◽  
Limnoperna Fortunei ◽  
Larval Size ◽  
Larval Stages ◽  
Positive Effects ◽  
Paraguay River ◽  
Body Sizes ◽  
Golden Mussel

AIM: The present study involved an analysis of the monthly variations in the population densities and body sizes of the different stages of planktonic larvae of the invasive golden mussel (Limnoperna fortunei), in the rivers Paraguay and Miranda; METHODS: The study was carried out between February 2004 and January 2005. Monthly collection of the plankton samples was accompanied by physical, chemical and biological analyses of the water; RESULTS: The Miranda River presented higher values of calcium, pH, alkalinity, conductivity and total phosphorous. Larval density varied from 0-24 individuals.L-1 in the Paraguay River, with a peak in March of 2004, while in the Miranda River, densities varied between 0-9 individuals.L-1 with a peak in February of 2004. No larvae were encountered during the coldest months, May and June. No significant correlations were found between environmental variables and larval density in either river. Only the valved larval stages were recorded. The "D" and veliger forms were most abundant; umbonate larvae were rare in the Miranda River samples. Mean body sizes of "D", veliger and umbonate larval stages were, respectively, 111, 135 and 152 µm, in the Paraguay River, and 112, 134 and 154 µm in the Miranda River. Principal Components Analysis indicated positive relationships between "D" larval stage size and the ratio between inorganic and organic suspended solids, while negative relationships were found between larval size and calcium and chlorophyll-<img border=0 width=7 height=8 src="/img/revistas/alb/2012nahead/ALB_AOP_230307car01.jpg">; CONCLUSIONS: The larvae were recorded in the plankton during most of the year, with the exception of the two colder months. Neither densities nor larval stage body sizes were significantly different between the two rivers. Possible positive effects of food and calcium concentrations on body size were not recorded. This species may be adapted to grow in environments with elevated sediment concentrations.

scholarly journals The scientific literature on Limnoperna fortunei (Dunker 1857) from 1982 to 2012

2014 ◽  
Vol 86 (3) ◽  
pp. 1373-1384 ◽  
Author(s):  
FABIANA G. BARBOSA
Keyword(s):  
Invasive Species ◽  
Aquatic Ecosystems ◽  
Observational Studies ◽  
Scientific Literature ◽  
Scientific Information ◽  
Scientometric Analysis ◽  
Limnoperna Fortunei ◽  
Freshwater Bivalve ◽  
Collaborative Studies ◽  
Golden Mussel

Limnoperna fortunei (golden mussel) is a freshwater bivalve native to Southeast Asia, but is becoming an invasive species in several aquatic ecosystems in the world. In this study, a scientometric analysis was performed to identify the patterns, trends and gaps of knowledge for this invasive species. A survey of the published literature was conducted using the database of the Thomson Institute for Scientific Information (ISI). A total of 107 papers were surveyed that were published between 1982 and 2012 in 60 journals. The number of papers on L. fortunei over the years has increased, especially within the last eight years of the study period. Argentina, Brazil, and Japan are the countries that contributed the most papers to the literature on invasive bivalve. The majority of papers were field-observational studies. Among some important gaps that need to be addressed are the relatively small number and/or lack of studies conducted in the native countries and in countries invaded by L. fortunei, the lack of internationally collaborative publications in these countries, as well as a low number of internationally collaborative studies.

Sign in / Sign up

Export Citation Format

Share Document