scholarly journals Author response: FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis

2014 ◽  
Author(s):  
Rainer Waadt ◽  
Kenichi Hitomi ◽  
Noriyuki Nishimura ◽  
Chiharu Hitomi ◽  
Stephen R Adams ◽  
...  
2013 ◽  
Vol 48 (9) ◽  
pp. 1210-1219 ◽  
Author(s):  
Muhammad Iqbal ◽  
Muhammad Ashraf

The objective of this work was to assess the regulatory effects of auxin-priming on gas exchange and hormonal homeostasis in spring wheat subjected to saline conditions. Seeds of MH-97 (salt-intolerant) and Inqlab-91 (salt-tolerant) cultivars were subjected to 11 priming treatments (three hormones x three concentrations + two controls) and evaluated under saline (15 dS m-1) and nonsaline (2.84 dS m-1) conditions. The priming treatments consisted of: 5.71, 8.56, and 11.42 × 10-4 mol L-1 indoleacetic acid; 4.92, 7.38, and 9.84 × 10-4 mol L-1 indolebutyric acid; 4.89, 7.34, and 9.79 × 10-4 mol L-1 tryptophan; and a control with hydroprimed seeds. A negative control with nonprimed seeds was also evaluated. All priming agents diminished the effects of salinity on endogenous abscisic acid concentration in the salt-intolerant cultivar. Grain yield was positively correlated with net CO2 assimilation rate and endogenous indoleacetic acid concentration, and it was negatively correlated with abscisic acid and free polyamine concentrations. In general, the priming treatment with tryptophan at 4.89 × 10-4 mol L-1 was the most effective in minimizing yield losses and reductions in net CO2 assimilation rate, under salt stress conditions. Hormonal homeostasis increases net CO2 assimilation rate and confers tolerance to salinity on spring wheat.


1981 ◽  
Vol 8 (5) ◽  
pp. 443 ◽  
Author(s):  
WJS Downton ◽  
BR Loveys

Changes in abscisic acid, phaseic acid, stomatal resistance, water potential, osmotic potential, turgor potential, proline, reducing sugars and ion content (Na+, K+, Cl-) in leaves from grapevines (Vitis vinifera L.) subjected to 0, 25, 50 or 100 mM NaCl (osmotic potentials of 0, - 0.1, - 0.2 and - 0.4 MPa, respectively) were monitored over a 3-week period. Abscisic acid concentration increased within 6 h for the 50 and 100 mM NaCl-treated vines. Proline did not accumulate until the next day for the 100 mM NaCl-treated plants and continued to accumulate for the duration of the experiment. Phaseic acid showed kinetics consistent with its being derived from abscisic acid. Stomatal resistance to water vapour exchange increased in the salt-treated plants over the course of the experiment despite a decline in abscisic acid concentration after the initial upsurge. Reducing sugar concentration showed an early upsurge, its contribution to osmotic readjustment being at least equal to that of accumulated Na+, K+ and Cl- the day after stress began. Potassium was preferentially accumulated over sodium into leaves during the first 8 days of the experiment and the sum of these two cations generally balanced accumulating chloride. Except for an initial loss of turgor in vines given 100 mM NaCl, turgor potential was maintained within 0.1 MPa of control plants for all of the treatments throughout the experiment.


2009 ◽  
Vol 183 (4) ◽  
pp. 1030-1042 ◽  
Author(s):  
Yinggao Liu ◽  
Lin Shi ◽  
Nenghui Ye ◽  
Rui Liu ◽  
Wensuo Jia ◽  
...  

2009 ◽  
Vol 31 (4) ◽  
pp. 825-831 ◽  
Author(s):  
Mokhtar Guerfel ◽  
Alexandros Beis ◽  
Tasos Zotos ◽  
Dalenda Boujnah ◽  
Mokhtar Zarrouk ◽  
...  

1987 ◽  
Vol 17 (5) ◽  
pp. 383-387 ◽  
Author(s):  
Pasi Puttonen

Spring-lifted seedlings were grown in pots in the field and, after a natural fall photoperiod, exposed to three 25-day cold (+4 °C) storage treatments and two lifting times, mid-November and mid-January. The storage treatments were light storage in pots, dark storage in pots, and bareroot storage in polyethylene bags in the dark. In a second experiment, an extended fall photoperiod treatment was applied to seedlings that were then stored in pots and subjected to the same light and dark treatments above. In both experiments, needle samples were taken four times during and after the treatments for abscisic acid assay. Abscisic acid concentrations were determined using gas liquid chromatography after purification with high performance liquid chromatography. Lifting times and storage treatments did not result in statistically significant differences in abscisic acid concentrations. However, there were treatment differences in characteristics of postplanting performance. Mid-November lifting resulted in reduced survival and a greater number of days to bud flush compared with the mid-January lifting results. The extended fall photoperiod material produced similar results to the natural fall photoperiod material. The failure to detect a relationship between needle abscisic acid concentration and seedling vigor may have been due to a transitory role of abscisic acid in the storage conditions studied. The quantification method for abscisic acid is insensitive and laborious for practical seedling testing.


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