scholarly journals Dpp from the anterior stripe of cells is crucial for the growth of the Drosophila wing disc

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Shinya Matsuda ◽  
Markus Affolter
Keyword(s):  
Null Allele ◽  
Growth Control ◽  
Wing Disc ◽  
The Other ◽  
Morphogen Gradient ◽  
Posterior Compartment ◽  
Time Points ◽  
Larval Stages ◽  
Drosophila Wing ◽  
Control Growth

The Dpp morphogen gradient derived from the anterior stripe of cells is thought to control growth and patterning of the Drosophila wing disc. However, the spatial-temporal requirement of dpp for growth and patterning remained largely unknown. Recently, two studies re-addressed this question. By generating a conditional null allele, one study proposed that the dpp stripe is critical for patterning but not for growth (Akiyama and Gibson, 2015). In contrast, using a membrane-anchored nanobody to trap Dpp, the other study proposed that Dpp dispersal from the stripe is required for patterning and also for medial wing disc growth, at least in the posterior compartment (Harmansa et al., 2015). Thus, growth control by the Dpp morphogen gradient remains under debate. Here, by removing dpp from the stripe at different time points, we show that the dpp stripe source is indeed required for wing disc growth, also during third instar larval stages.

Regeneration from duplicating fragments of the Drosophila wing disc

Development ◽  
1981 ◽  
Vol 66 (1) ◽  
pp. 117-126
Author(s):  
Jane Karlsson ◽  
R. J. Smith
Keyword(s):  
Imaginal Disc ◽  
General Rule ◽  
Wing Disc ◽  
The Other ◽  
Posterior Compartment ◽  
Drosophila Wing ◽  
Polar Coordinate ◽  
New Type ◽  
Coordinate Model

It is a general rule that of two complementary Drosophila imaginal disc fragments, one regenerates and the other duplicates. This paper reports an investigation of an exception to this rule. Duplicating fragments from the periphery of the wing disc which lacked presumptive notum were found to regenerate notum structures during and after duplication. The propensity for this was greatest in fragments lying close to the presumptive notum, with the exception of a fragment confined to the posterior compartment, which did not regenerate notum. Structures were added sequentially, and regeneration stopped once most of the notum was present. These results are not easily explained by the polar coordinate model, which states that regeneration cannot occur from duplicating fragments. Since compartments appear to be involved in this type of regeneration as in others, it is suggested that a new type of model is required, one which permits simultaneous regeneration and duplication, and assigns a major role to compartments.

Quantitative EM of gap junction distribution in mutant drosophila wing discs

1983 ◽  
Vol 41 ◽  
pp. 720-721
Author(s):  
J.S. Ryerse
Keyword(s):  
Gap Junctions ◽  
Growth Control ◽  
Intercellular Junctions ◽  
Wing Disc ◽  
En Bloc ◽  
Metabolic Cooperation ◽  
Drosophila Wing ◽  
Adult Wing ◽  
Wing Discs ◽  
Wing Pouch

Gap junctions are intercellular junctions found in both vertebrates and invertebrates through which ions and small molecules can pass. Their distribution in tissues could be of critical importance for ionic coupling or metabolic cooperation between cells or for regulating the intracellular movement of growth control and pattern formation factors. Studies of the distribution of gap junctions in mutants which develop abnormally may shed light upon their role in normal development. I report here the distribution of gap junctions in the wing pouch of 3 Drosophila wing disc mutants, vg (vestigial) a cell death mutant, 1(2)gd (lethal giant disc) a pattern abnormality mutant and 1(2)gl (lethal giant larva) a neoplastic mutant and compare these with wildtype wing discs.The wing pouch (the anlagen of the adult wing blade) of a wild-type wing disc is shown in Fig. 1 and consists of columnar cells (Fig. 5) joined by gap junctions (Fig. 6). 14000x EMs of conventionally processed, UA en bloc stained, longitudinally sectioned wing pouches were enlarged to 45000x with a projector and tracings were made on which the lateral plasma membrane (LPM) and gap junctions were marked.

scholarly journals The function of engrailed and the specification of Drosophila wing pattern

Development ◽  
1995 ◽  
Vol 121 (10) ◽  
pp. 3447-3456 ◽  
Author(s):  
I. Guillen ◽  
J.L. Mullor ◽  
J. Capdevila ◽  
E. Sanchez-Herrero ◽  
G. Morata ◽  
...  
Keyword(s):  
Wing Disc ◽  
Gene Products ◽  
Wing Pattern ◽  
Related Gene ◽  
Posterior Compartment ◽  
Anterior Compartment ◽  
Drosophila Wing ◽  
Partial Inactivation ◽  
Autoregulatory Mechanism ◽  
Adult Drosophila

The adult Drosophila wing (as the other appendages) is subdivided into anterior and posterior compartments that exhibit characteristic patterns. The engrailed (en) gene has been proposed to be paramount in the specification of the posterior compartment identity. Here, we explore the adult en function by targeting its expression in different regions of the wing disc. In the anterior compartment, ectopic en expression gives rise to the substitution of anterior structures by posterior ones, thus demonstrating its role in specification of posterior patterns. The en-expressing cells in the anterior compartment also induce high levels of the hedgehog (hh) and decapentaplegic (dpp) gene products, which results in local duplications of anterior patterns. Besides, hh is able to activate en and the engrailed-related gene invected (inv) in this compartment. In the posterior compartment we find that elevated levels of en product result in partial inactivation of the endogenous en and inv genes, indicating the existence of a negative autoregulatory mechanism. We propose that en has a dual role: a general one for patterning of the appendage, achieved through the activation of secreted proteins like hh and dpp, and a more specific one, determining posterior identity, in which the inv gene may be implicated.

scholarly journals Free Extracellular Diffusion Creates the Dpp Morphogen Gradient of the Drosophila Wing Disc

2012 ◽  
Vol 22 (8) ◽  
pp. 668-675 ◽  
Author(s):  
Shaohua Zhou ◽  
Wing-Cheong Lo ◽  
Jeffrey L. Suhalim ◽  
Michelle A. Digman ◽  
Enrico Gratton ◽  
...  
Keyword(s):  
Wing Disc ◽  
Morphogen Gradient ◽  
Drosophila Wing

scholarly journals 2PT220 Growth control mechanism in the Drosophila wing disc(The 50th Annual Meeting of the Biophysical Society of Japan)

2012 ◽  
Vol 52 (supplement) ◽  
pp. S142
Author(s):  
Ken-ichi Hironaka ◽  
Yoh Iwasa ◽  
Yoshihiro Morishita
Keyword(s):  
Annual Meeting ◽  
Control Mechanism ◽  
Growth Control ◽  
Wing Disc ◽  
Drosophila Wing ◽  
Biophysical Society

scholarly journals Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc

2020 ◽  
Author(s):  
Shinya Matsuda ◽  
Jonas V. Schaefer ◽  
Yusuke Mii ◽  
Yutaro Hori ◽  
Dimitri Bieli ◽  
...  
Keyword(s):  
Positional Information ◽  
Wing Disc ◽  
Morphogen Gradients ◽  
Drosophila Wing ◽  
Control Growth ◽  
Protein Functions ◽  
Underlying Mechanisms ◽  
Anterior Patterning ◽  
Wing Growth ◽  
And Control

SummaryMorphogen gradients provide positional information and control growth in developing tissues, but the underlying mechanisms remain largely unknown due to lack of tools manipulating morphogen gradients. Here, we generate two synthetic protein binder tools manipulating different parameters of Decapentaplegic (Dpp), a morphogen thought to control Drosophila wing disc patterning and growth by dispersal; while HA trap blocks Dpp dispersal, Dpp trap blocks Dpp dispersal and signaling in the source cells. Using these tools, we found that while posterior patterning and growth require Dpp dispersal, anterior patterning and growth largely proceed without Dpp dispersal. We show that dpp transcriptional refinement from an initially uniform to a localized expression and persistent signaling in transient dpp source cells render the anterior compartment robust to blocking Dpp dispersal. Furthermore, neither Dpp dispersal nor signaling is critical for lateral wing growth. These results challenge Dpp dispersal-centric mechanisms, and demonstrate the utility of customized protein binder tools to dissect protein functions.

scholarly journals Single-cell transcriptomics of the Drosophila wing disc reveals instructive epithelium-to-myoblast interactions

2020 ◽  
Author(s):  
Nicholas J. Everetts ◽  
Melanie I. Worley ◽  
Riku Yasutomi ◽  
Nir Yosef ◽  
Iswar K. Hariharan
Keyword(s):  
Gene Expression ◽  
Single Cell ◽  
Wing Disc ◽  
Transcriptional Program ◽  
Time Points ◽  
Drosophila Wing ◽  
Global View ◽  
Direct Flight ◽  
A Cell ◽  
Flight Muscles

AbstractIn both vertebrates and invertebrates, generating a functional appendage requires interactions between ectoderm-derived epithelia and mesoderm-derived cells. To investigate such interactions, we used single-cell transcriptomics to generate a cell atlas of the Drosophila wing disc at two time points during development. Using these data, we investigate gene expression using a multi-layered model of the wing disc and catalogued ligand-receptor pairs that could mediate signaling between epithelial cells and adult muscle precursors (AMPs). We found that localized expression of the FGF ligands, Thisbe and Pyramus, in the disc epithelium regulates the number and location of the AMPs. In addition, Hedgehog ligand from the epithelium activates a specific transcriptional program within adjacent AMP cells, which is critical for proper formation of a subset of the direct flight muscles. More generally, our annotated atlas provides a global view of potential cell-cell interactions between subpopulations of epithelial and myogenic cells.

scholarly journals Single-cell transcriptomics of the Drosophila wing disc reveals instructive epithelium-to-myoblast interactions

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Nicholas J Everetts ◽  
Melanie I Worley ◽  
Riku Yasutomi ◽  
Nir Yosef ◽  
Iswar K Hariharan
Keyword(s):  
Gene Expression ◽  
Single Cell ◽  
Developmental Time ◽  
Wing Disc ◽  
Fibroblast Growth ◽  
Transcriptional Program ◽  
Time Points ◽  
Drosophila Wing ◽  
Direct Flight ◽  
Flight Muscles

In both vertebrates and invertebrates, generating a functional appendage requires interactions between ectoderm-derived epithelia and mesoderm-derived cells. To investigate such interactions, we used single-cell transcriptomics to generate a temporal cell atlas of theDrosophilawing disc from two developmental time points. Using these data, we visualized gene expression using a multilayered model of the wing disc and cataloged ligand–receptor pairs that could mediate signaling between epithelial cells and adult muscle precursors (AMPs). We found that localized expression of the fibroblast growth factor ligands, Thisbe and Pyramus, in the disc epithelium regulates the number and location of the AMPs. In addition, Hedgehog ligand from the epithelium activates a specific transcriptional program within adjacent AMP cells, defined by AMP-specific targetsNeurotactinandmidline, that is critical for proper formation of direct flight muscles. More generally, our annotated temporal cell atlas provides an organ-wide view of potential cell–cell interactions between epithelial and myogenic cells.

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