scholarly journals Cdc13 is predominant over Stn1 and Ten1 in preventing chromosome end fusions

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Zhi-Jing Wu ◽  
Jia-Cheng Liu ◽  
Xin Man ◽  
Xin Gu ◽  
Ting-Yi Li ◽  
...  
Keyword(s):  
Budding Yeast ◽  
Predominant Role ◽  
Chromosome Fusion ◽  
Linear Chromosome ◽  
Chromosomal Stability ◽  
Lethal Phenotype ◽  
Telomere Protection ◽  
End To End ◽  
Telomere Erosion

Telomeres define the natural ends of eukaryotic chromosomes and are crucial for chromosomal stability. The budding yeast Cdc13, Stn1 and Ten1 proteins form a heterotrimeric complex, and the inactivation of any of its subunits leads to a uniformly lethal phenotype due to telomere deprotection. Although Cdc13, Stn1 and Ten1 seem to belong to an epistasis group, it remains unclear whether they function differently in telomere protection. Here, we employed the single-linear-chromosome yeast SY14, and surprisingly found that the deletion of CDC13 leads to telomere erosion and intrachromosome end-to-end fusion, which depends on Rad52 but not Yku. Interestingly, the emergence frequency of survivors in the SY14 cdc13Δ mutant was ~29 fold higher than that in either the stn1Δ or ten1Δ mutant, demonstrating a predominant role of Cdc13 in inhibiting telomere fusion. Chromosomal fusion readily occurred in the telomerase-null SY14 strain, further verifying the default role of intact telomeres in inhibiting chromosome fusion.

scholarly journals Assess the Effectiveness of protective Concrete coatings of microbiological sulfuric acid Aggression

2019 ◽  
Vol 97 ◽  
pp. 02022
Author(s):  
Viktor Sopov ◽  
Julia Danchenko ◽  
Ekaterina Latorez
Keyword(s):  
Sulfuric Acid ◽  
Building Structures ◽  
Predominant Role ◽  
Microbiological Processes ◽  
Repair Materials ◽  
Bactericidal Properties ◽  
Mineral Acids ◽  
Concrete Building ◽  
Modified Epoxy

Specification of corrosion processes in sewerage networks, the predominant role of microbiological processes in them requires the protection of concrete with materials resistant to specific biogenic sulfuric acid aggression. The effective protective materials, in this case, are those that, in addition to acid-proofing materials, have bactericidal properties. Polymer composite materials (PCM) of cold curing based on modified epoxy-resin, resins with dispersed oxides as fillers are widely used in construction. Unique properties and simple technologies make it possible to use them as injection formulations for repair, restoration and restoration of building structures and structures, waterproofing and facing materials, for impregnating, gluing and coating metal, wooden, brick and concrete building structures and parts. In the reconstruction and restoration of sewerage collectors operating under conditions of exposure to liquid biochemically aggressive media with mineral acids and organic substances, particular attention is paid to the permeability of repair materials. Based on the results of the study, PCM compositions were chosen whose estimated lifetime is about 20 years, ensuring reliable protection of the sewage collectors from corrosion.

scholarly journals Predominant role of alpha 4-integrins for distinct steps of lymphoma metastasis.

1996 ◽  
Vol 93 (10) ◽  
pp. 4821-4826 ◽  
Author(s):  
U. Gosslar ◽  
P. Jonas ◽  
A. Luz ◽  
A. Lifka ◽  
D. Naor ◽  
...  
Keyword(s):  
Predominant Role

Abstract 207: Unc-51-like Kinase 1 (ULK1) Plays A Crucial Role In Mitophagy In Cardiomyocytes

2014 ◽  
Vol 115 (suppl_1) ◽  
Author(s):  
Toshiro Saito ◽  
Junichi Sadoshima
Keyword(s):  
Glucose Deprivation ◽  
High Ratio ◽  
Control Mechanisms ◽  
Oxygen Species ◽  
Predominant Role ◽  
Mitochondrial Quality Control ◽  
Over Expression ◽  
Pathological Conditions

The mitochondrion is an essential organelle that supplies ATP in cardiomyocytes (CMs). However, damaged mitochondria are harmful via the production of reactive oxygen species and induction of apoptosis in pathological conditions. Therefore, quality of mitochondria should be controlled tightly through various mitochondrial quality control mechanisms. Mitochondrial autophagy (mitophagy) is considered an integral part of this mechanism, and recent investigations uncovered the role of PINK1 and Parkin in mitophagy. However, these observations were made under artificial conditions, such as over-expression of Parkin or treatment with CCCP, and thus the precise mechanism has not been fully elucidated in more pathophysiologically relevant conditions. Recent evidence suggests that mitophagy can take place independently of ATG7, a molecule essential for the conventional form of autophagy, and that this form of autophagy is ULK1-dependent. We investigated the role of ULK1 and ATG7 in mediating mitophagy using mitochondria-targeted Keima (Mito-Keima) in cultured rat neonatal CMs. Keima has a bimodal excitation spectrum peaking at 440 and 560 nm, corresponding to the neutral and acidic pH, respectively. In CMs transfected with Mito-Keima, the fluorescent dots with a high 560nm/440nm ratio represent the mitochondria incorporated into autolysosomes which indicate mitophagy. Here we report that ULK1 plays a more predominant role in glucose deprivation (GD) -induced mitophagy than ATG7. Control CMs exhibited 8.7±1.0 % of the area of high-ratio dots per cells after GD. Knockdown of ULK1 significantly reduced the area to 2.3±0.9 % in CMs after GD (p<0.01, vs sh-Control). The reduction was significantly greater in CMs with knockdown of ULK1 than that of ATG7 (7.0±1.6 %, p<0.05, sh-ULK1 vs sh-ATG7). In addition, knockdown of Beclin1 and Drp1 also significantly decreased the area of high-ratio dots (about 1.0 % and 0.5 %, respectively). Overexpression of ULK1 was sufficient to induce mitophagy without starvation, whereas that of ATG7 was not. These results suggest that ULK1, Beclin1 and Drp1 play an essential role in mediating GD-induced mitophagy in CMs.

The Clinton Administration and Africa: Role of Congress and the Africa Subcommittees

1998 ◽  
Vol 26 (2) ◽  
pp. 14-16
Author(s):  
Daniel P. Volman
Keyword(s):  
Cold War ◽  
Reagan Administration ◽  
Clinton Administration ◽  
Executive Branch ◽  
Predominant Role ◽  
Government Relations ◽  
Foreign Operations ◽  
The Cold War ◽  
The U.S

Studies of U.S. government relations with Africa have generally focused on the role of the executive branch, specifically by examining and analyzing the views and activities of administration officials and the members of executive branch bureaucracies. This is only natural, given the predominant role that the executive branch has historically played in the development and implementation of U.S. policy toward the continent. However, the U.S. Congress has always played an important role in determining U.S. policy toward Africa due to its constitutional authority over the appropriation and authorization of funding for all foreign operations conducted by the executive branch. Furthermore, Congress enacted legislation on several occasions during the Cold War period that directly affected U.S. policy. For example, Congress approved the Clark Amendment prohibiting U.S. intervention in Angola (although it later voted to repeal the amendment) and also passed the 1986 Comprehensive Anti-Apartheid Act, which imposed sanctions on South Africa over the veto of the Reagan administration.

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