scholarly journals Decision letter: Cellular taxonomy and spatial organization of the murine ventral posterior hypothalamus

2020 ◽  
Keyword(s):  
Spatial Organization ◽  
Posterior Hypothalamus

scholarly journals Cellular taxonomy and spatial organization of the ventral posterior hypothalamus reveals neuroanatomical parcellation of the mammillary bodies

2020 ◽  
Author(s):  
Laura E. Mickelsen ◽  
William F. Flynn ◽  
Kristen Springer ◽  
Lydia Wilson ◽  
Eric J. Beltrami ◽  
...  
Keyword(s):  
Single Cell ◽  
Spatial Organization ◽  
Single Cells ◽  
Neuronal Cell ◽  
Cell Types ◽  
Posterior Hypothalamus ◽  
Neuronal Populations ◽  
Mammillary Bodies ◽  
Distinct Cell ◽  
And Behavior

ABSTRACTThe ventral posterior hypothalamus (VPH) is an anatomically complex brain region implicated in arousal, reproduction, energy balance and memory processing. However, neuronal cell type diversity within the VPH is poorly understood, an impediment to deconstructing the roles of distinct VPH circuits in physiology and behavior. To address this question, we employed a droplet-based single cell RNA sequencing (scRNA-seq) approach to systematically classify molecularly distinct cell types in the mouse VPH. Analysis of >16,000 single cells revealed 20 neuronal and 18 non-neuronal cell populations, defined by suites of discriminatory markers. We validated differentially expressed genes in a selection of neuronal populations through fluorescence in situ hybridization (FISH). Focusing on the mammillary bodies (MB), we discovered transcriptionally-distinct clusters that exhibit a surprising degree of segregation within neuroanatomical subdivisions of the MB, while genetically-defined MB cell types project topographically to the anterior thalamus. This single cell transcriptomic atlas of cell types in the VPH provides a detailed resource for interrogating the circuit-level mechanisms underlying the diverse functions of VPH circuits in health and disease.

scholarly journals Author response: Cellular taxonomy and spatial organization of the murine ventral posterior hypothalamus

2020 ◽  
Author(s):  
Laura E Mickelsen ◽  
William F Flynn ◽  
Kristen Springer ◽  
Lydia Wilson ◽  
Eric J Beltrami ◽  
...  
Keyword(s):  
Spatial Organization ◽  
Posterior Hypothalamus ◽  
Author Response

scholarly journals Cellular taxonomy and spatial organization of the murine ventral posterior hypothalamus

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Laura E Mickelsen ◽  
William F Flynn ◽  
Kristen Springer ◽  
Lydia Wilson ◽  
Eric J Beltrami ◽  
...  
Keyword(s):  
Single Cell ◽  
Spatial Organization ◽  
Single Cells ◽  
Neuronal Cell ◽  
Cell Types ◽  
Posterior Hypothalamus ◽  
Cell Populations ◽  
Neuronal Populations ◽  
Distinct Cell ◽  
And Behavior

The ventral posterior hypothalamus (VPH) is an anatomically complex brain region implicated in arousal, reproduction, energy balance, and memory processing. However, neuronal cell type diversity within the VPH is poorly understood, an impediment to deconstructing the roles of distinct VPH circuits in physiology and behavior. To address this question, we employed a droplet-based single-cell RNA sequencing (scRNA-seq) approach to systematically classify molecularly distinct cell populations in the mouse VPH. Analysis of >16,000 single cells revealed 20 neuronal and 18 non-neuronal cell populations, defined by suites of discriminatory markers. We validated differentially expressed genes in selected neuronal populations through fluorescence in situ hybridization (FISH). Focusing on the mammillary bodies (MB), we discovered transcriptionally-distinct clusters that exhibit neuroanatomical parcellation within MB subdivisions and topographic projections to the thalamus. This single-cell transcriptomic atlas of VPH cell types provides a resource for interrogating the circuit-level mechanisms underlying the diverse functions of VPH circuits.

Computational Micrograph Registration with Sieve Processes

1996 ◽  
Vol 54 ◽  
pp. 440-441
Author(s):  
P.J. Phillips ◽  
J. Huang ◽  
S. M. Dunn
Keyword(s):  
Planar Graph ◽  
Efficient Algorithm ◽  
Spatial Organization ◽  
Spatial Arrangement ◽  
Stereo Image ◽  
Image Model ◽  
Geometric Invariants ◽  
Point Set

In this paper we present an efficient algorithm for automatically finding the correspondence between pairs of stereo micrographs, the key step in forming a stereo image. The computation burden in this problem is solving for the optimal mapping and transformation between the two micrographs. In this paper, we present a sieve algorithm for efficiently estimating the transformation and correspondence.In a sieve algorithm, a sequence of stages gradually reduce the number of transformations and correspondences that need to be examined, i.e., the analogy of sieving through the set of mappings with gradually finer meshes until the answer is found. The set of sieves is derived from an image model, here a planar graph that encodes the spatial organization of the features. In the sieve algorithm, the graph represents the spatial arrangement of objects in the image. The algorithm for finding the correspondence restricts its attention to the graph, with the correspondence being found by a combination of graph matchings, point set matching and geometric invariants.

Caveolin-1, galectin-3 and lipid raft domains in cancer cell signalling

2015 ◽  
Vol 57 ◽  
pp. 189-201 ◽  
Author(s):  
Jay Shankar ◽  
Cecile Boscher ◽  
Ivan R. Nabi
Keyword(s):  
Plasma Membrane ◽  
Lipid Rafts ◽  
Cancer Cell ◽  
Cellular Response ◽  
Spatial Organization ◽  
Essential Feature ◽  
Cell Signalling ◽  
Coated Pits ◽  
Signalling Molecules ◽  
Raft Domains

Spatial organization of the plasma membrane is an essential feature of the cellular response to external stimuli. Receptor organization at the cell surface mediates transmission of extracellular stimuli to intracellular signalling molecules and effectors that impact various cellular processes including cell differentiation, metabolism, growth, migration and apoptosis. Membrane domains include morphologically distinct plasma membrane invaginations such as clathrin-coated pits and caveolae, but also less well-defined domains such as lipid rafts and the galectin lattice. In the present chapter, we will discuss interaction between caveolae, lipid rafts and the galectin lattice in the control of cancer cell signalling.

The Social Construction of a Perfect Market: The Strawberry Auction at Fontaines-en-Sologne

2010 ◽  
pp. 73-89
Author(s):  
M.-F. Garcia
Keyword(s):  
Economic Theory ◽  
Social Construction ◽  
Empirical Study ◽  
Spatial Organization ◽  
Frame Of Reference ◽  
Social Conditions ◽  
Invisible Hand ◽  
The Social ◽  
Per Se

The article examines social conditions and mechanisms of the emergence in 1982 of a «Dutch» strawberry auction in Fontaines-en-Sologne, France. Empirical study of this case shows that perfect market does not arise per se due to an «invisible hand». It is a social construction, which could only be put into effect by a hard struggle between stakeholders and large investments of different forms of capital. Ordinary practices of the market dont differ from the predictions of economic theory, which is explained by the fact that economic theory served as a frame of reference for the designers of the auction. Technological and spatial organization as well as principal rules of trade was elaborated in line with economic views of perfect market resulting in the correspondence between theory and reality.

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