scholarly journals Decision letter: Ribosome recycling is not critical for translational coupling in Escherichia coli

2020 ◽  
Author(s):  
Pavel V Baranov ◽  
Joseph T Wade
Keyword(s):  
Escherichia Coli ◽  
Translational Coupling

scholarly journals Regulation of Escherichia coli secA by Cellular Protein Secretion Proficiency Requires an Intact Gene X Signal Sequence and an Active Translocon

1998 ◽  
Vol 180 (19) ◽  
pp. 5240-5242 ◽  
Author(s):  
Donald Oliver ◽  
Jessica Norman ◽  
Shameema Sarker
Keyword(s):  
Escherichia Coli ◽  
Protein Secretion ◽  
Signal Sequence ◽  
Cellular Protein ◽  
X Protein ◽  
Translational Coupling ◽  
Intact Gene

ABSTRACT secA is translationally regulated by the protein secretion proficiency state of the Escherichia coli cell. This regulation was explored by making signal sequence mutations in the gene upstream of secA, gene X, which promotessecA translational coupling. Gene X signal sequence mutants were constitutive for secA expression, whileprlA alleles partially restored secAregulation. These results show that interaction of the pre-gene X protein with the translocon is required for proper secAregulation. Furthermore, gene X signal sequence mutations disruptedsecA regulation only in the cis configuration. We propose that nascent pre-gene X protein interacts with the translocon during its secretion to constitute the secretion sensor.

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