Localization of the target site for translational regulation of the L11 operon and direct evidence for translational coupling in Escherichia coli

Cell ◽  
1983 ◽  
Vol 34 (3) ◽  
pp. 979-988 ◽  
Author(s):  
Gail Baughman ◽  
Masayasu Nomura
2017 ◽  
Vol 15 (5) ◽  
pp. 324-330 ◽  
Author(s):  
Yanqing Zhang ◽  
Zhengtao Xiao ◽  
Qin Zou ◽  
Jianhuo Fang ◽  
Qifan Wang ◽  
...  

2005 ◽  
Vol 49 (1) ◽  
pp. 441-443 ◽  
Author(s):  
J. Gangoue-Pieboji ◽  
V. Miriagou ◽  
S. Vourli ◽  
E. Tzelepi ◽  
P. Ngassam ◽  
...  

ABSTRACT CTX-M-15-producing Klebsiella pneumoniae and Escherichia coli emerged recently in Cameroon. CTX-M-15 was encoded by two different multiresistance plasmids, of which one carried an ISEcp1-bla CTX-M-15 element flanked by a 5-bp target site duplication and inserted within a Tn2-derived sequence. A truncated form of this element in the second plasmid was identified.


2020 ◽  
Vol 6 (6) ◽  
pp. eaax0947 ◽  
Author(s):  
J. Mark Kim ◽  
Mayra Garcia-Alcala ◽  
Enrique Balleza ◽  
Philippe Cluzel

The classic picture of flagellum biosynthesis in Escherichia coli, inferred from population measurements, depicts a deterministic program where promoters are sequentially up-regulated and are maintained steadily active throughout exponential growth. However, complex regulatory dynamics at the single-cell level can be masked by bulk measurements. Here, we discover that in individual E. coli cells, flagellar promoters are stochastically activated in pulses. These pulses are coordinated within specific classes of promoters and comprise “on” and “off” states, each of which can span multiple generations. We demonstrate that in this pulsing program, the regulatory logic of flagellar assembly dictates which promoters skip pulses. Surprisingly, pulses do not require specific transcriptional or translational regulation of the flagellar master regulator, FlhDC, but instead appears to be essentially governed by an autonomous posttranslational circuit. Our results suggest that even topologically simple transcriptional networks can generate unexpectedly rich temporal dynamics and phenotypic heterogeneities.


2010 ◽  
Vol 285 (14) ◽  
pp. 10690-10702 ◽  
Author(s):  
Anders Boysen ◽  
Jakob Møller-Jensen ◽  
Birgitte Kallipolitis ◽  
Poul Valentin-Hansen ◽  
Martin Overgaard

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