scholarly journals Identificación molecular de aislamientos clínicos de Leishmania spp. procedentes de Colombia con base en el gen hsp70

Biomédica ◽  
2016 ◽  
Vol 36 ◽  
Author(s):  
Ana M. Montalvo ◽  
Jorge Fraga ◽  
Ivón Montano ◽  
Lianet Monzote ◽  
Gert Van der Auwera ◽  
...  
Keyword(s):  
Leishmania Braziliensis ◽  
Leishmania Panamensis ◽  
Leishmania Spp ◽  
Leishmania Guyanensis

<p><strong>Introducción.</strong> La leishmaniasis es una enfermedad de alta prevalencia en Colombia, donde al menos seis especies diferentes pueden causar una variada presentación clínica en el humano. La tipificación de la especie es importante no solo desde el punto de vista epidemiológico, sino en el diagnóstico, dado que el tratamiento y el esquema de tratamiento pueden variar dependiendo de la especie identificada. Para la identificación se han utilizado distintas alternativas metodológicas, con variable poder discriminatorio.</p><p><strong>Objetivo.</strong> Realizar la identificación molecular de especies de <em>Leishmania spp.</em> mediante<strong> </strong>la amplificación molecular de un fragmento del gen <em>hsp</em>70.</p><p><strong>Materiales y métodos.</strong> Se realizó la amplificación molecular de un fragmento del gen <em>hsp</em>70: PCR-<em>hsp</em>70 (siglas en inglés) seguida del análisis del tamaño de los fragmentos de restricción (RFLP siglas en inglés), a 81 aislamientos clínicos de <em>Leishmania spp.</em>, provenientes de pacientes con enfermedad cutánea y mucocutánea, en los cuales se identificaron las especies presentes.</p><p><strong>Resultados.</strong> Se obtuvo un único producto de amplificación para el total de muestras analizadas. La restricción enzimática permitió identificar 70 aislamientos con un patrón de bandas correspondiente a <em>Leishmania braziliensis, </em>que incluye<em> </em>dos patrones diferentes (62 y 8 aislamientos respectivamente); 9 aislamientos compatibles con <em>Leishmania panamensis</em> y 2 con <em>Leishmania guyanensis</em>. El origen geográfico de los aislamientos concuerda con reportes anteriores sobre la distribución de las especies correspondientes.</p><p><strong>Conclusiones.</strong> La técnica de PCR-<em>hsp</em>70/RFLP utilizada es útil para identificar especies de <em>Leishmania</em> aisladas de muestras clínicas de Colombia que puede ser aplicable también al estudio de cepas provenientes de vectores y reservorios con importancia epidemiológica.</p>

scholarly journals Leishmaniasis recidivans in Ethiopia: cutaneous and mucocutaneous features

2017 ◽  
Vol 11 (01) ◽  
pp. 106-110 ◽  
Author(s):  
Federica Dassoni ◽  
Frehiwot Daba ◽  
Bernard Naafs ◽  
Aldo Morrone
Keyword(s):  
Cutaneous Leishmaniasis ◽  
New World ◽  
Leishmania Braziliensis ◽  
Leishmania Tropica ◽  
Clinical Form ◽  
Leishmania Aethiopica ◽  
Mucosal Involvement ◽  
Leishmania Panamensis ◽  
First Time ◽  
Leishmania Guyanensis

Cutaneous leishmaniasis (CL) is endemic in Ethiopia. An unusual clinical form of this disease is leishmaniasis recidivans (LR), a prolonged, relapsing form of cutaneous leishmaniasis resembling tuberculosis of the skin that may persist for many years with a chronic and relapsing course. This rare variant has been shown to be caused by Leishmania tropica species in the Old World and by Leishmania braziliensis, Leishmania amazonensis, Leishmania panamensis, and Leishmania guyanensis in the New World, as reported in various studies. To our knowledge, there are no reports from Ethiopia, and mucocutaneous involvement of LR has not been described to date. This was a retrospective analysis of the patients seen at the Italian Dermatological Center in Mekelle on the Tigrean highlands over a three-year period (2008–2011). Seven patients with typical clinical features of LR were seen. Two of them presented with signs of mucosal involvement. To date, Leishmania aethiopica is shown to be the only species causing CL that is endemic in the Ethiopian highlands. Therefore, it had to be assumed that the lesions in these patients were caused by this species. The aims of this communication are to report, for the first time, the presence of LR, most likely due to Leishmania aethiopica, in Ethiopia, and to report mucosal involvement in this rare clinical form of CL.

scholarly journals NEW PRIMERS FOR DETECTION OF Leishmania infantum USING POLYMERASE CHAIN REACTION

2015 ◽  
Vol 57 (5) ◽  
pp. 377-383 ◽  
Author(s):  
Kézia Peres GUALDA ◽  
Lílian Mathias MARCUSSI ◽  
Herintha Coeto NEITZKE-ABREU ◽  
Sandra Mara Alessi ARISTIDES ◽  
Maria Valdrinez Campana LONARDONI ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Leishmania Infantum ◽  
Leishmania Major ◽  
High Sensitivity ◽  
Leishmania Braziliensis ◽  
Chain Reaction ◽  
Serological Tests ◽  
Leishmania Panamensis ◽  
Polymerase Chain ◽  
Leishmania Guyanensis

SUMMARY Leishmania infantum causes visceral leishmaniasis (VL) in the New World. The diagnosis of VL is confirmed by parasitological and serological tests, which are not always sensitive or specific. Our aim was to design new primers to perform a Polymerase Chain Reaction (PCR) for detecting L. infantum. Sequences of the minicircle kinetoplast DNA (kDNA) were obtained from GenBank, and the FLC2/RLC2 primers were designed. Samples of DNA from L. infantum, Leishmania amazonensis, Leishmania braziliensis, Leishmania guyanensis, Leishmania naiffi, Leishmania lainsoni, Leishmania panamensis, Leishmania major and Trypanosoma cruzi were used to standardize the PCR. PCR with FLC2/RLC2 primers amplified a fragment of 230 bp and the detection limit was 0.2 fg of L. infantum DNA. Of the parasite species assayed, only L. infantum DNA was amplified. After sequencing, the fragment was aligned to GenBank sequences, and showed (99%) homology with L. infantum. In the analysis of blood samples and lesion biopsy from a dog clinically suspected to have VL, the PCR detected DNA from L. infantum. In biopsy lesions from humans and dogs with cutaneous leishmaniasis, the PCR was negative. The PCR with FLC2/RLC2 primers showed high sensitivity and specificity, and constitutes a promising technique for the diagnosis of VL.

scholarly journals Imported leishmaniasis in travelers: a 7-year retrospective from a Parisian hospital in France

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Nesrine Aissaoui ◽  
Samia Hamane ◽  
Maud Gits-Muselli ◽  
Antoine Petit ◽  
Mazouz Benderdouche ◽  
...  
Keyword(s):  
North Africa ◽  
Leishmania Major ◽  
Sub Saharan Africa ◽  
Leishmania Braziliensis ◽  
Diagnostic Strategy ◽  
Leishmania Mexicana ◽  
Leishmania Panamensis ◽  
Sub Saharan ◽  
Specific Symptoms ◽  
Leishmania Guyanensis

Abstract Background Leishmaniases are regularly seen in non-endemic areas due to the increase of international travels. They include cutaneous leishmaniases (CL) and mucocutaneous (MC) caused by different Leishmania species, and visceral leishmaniases (VL) which present with non-specific symptoms. Methods We reviewed all consecutive leishmaniasis cases seen between September 2012 and May 2020. The diagnostic strategy included microscopy after May-Grünwald-Giemsa staining, a diagnostic quantitative PCR (qPCR) assay, and species identification based on sequencing of the cytochrome b gene. Results Eighty-nine patients had a definitive leishmaniasis diagnosis. Nine patients had VL with Leishmania infantum. Eighty patients had CL. Twelve patients acquired CL after trips in Latin America (7 Leishmania guyanensis, 2 Leishmania braziliensis, 2 Leishmania mexicana, and 1 Leishmania panamensis). Species could be identified in 63 of the 68 CLs mainly after travel in North Africa (59%) with Leishmania major (65%), Leishmania tropica/killicki (24%), and L. infantum (11%), or in West Sub-Saharan Africa (32%), all due to L. major. The median day between appearance of the lesions and diagnosis was 90 [range 60–127]. Conclusions Our diagnostic strategy allows both positive diagnoses and species identifications. Travelers in West Sub-Saharan Africa and North Africa should be better aware of the risk of contracting leishmananiasis.

scholarly journals Primera caracterización molecular de Leishmania infantum en pacientes con leishmaniosis visceral de la Provincia de Misiones, Argentina

Biomédica ◽  
2019 ◽  
Vol 39 (2) ◽  
pp. 405-414 ◽  
Author(s):  
Ricardo G. Díaz ◽  
Karina A. Salvatierra ◽  
Gustavo A. Silva ◽  
Enrique J. Deschutter ◽  
Fernando J. Bornay-Llinares ◽  
...  
Keyword(s):  
Leishmania Infantum ◽  
Leishmania Braziliensis ◽  
Leishmania Spp

Introducción. La leishmaniosis cutánea por Leishmania braziliensis ha sido tradicionalmente endémica en Argentina y se han sido descritos casos de compromiso visceral después de una leishmaniosis cutánea inicial. La leishmaniosis visceral emergió en Argentina en el año 2006 en la ciudad de Posadas, provincia de Misiones, afectando tanto a humanos como a perros.Objetivo. Identificar el agente etiológico a nivel de especie de los pacientes diagnosticados con leishmaniosis visceral en Misiones y describir sus características clínicoepidemiológicas.Materiales y métodos. Se estudió una serie de 24 pacientes con diagnóstico confirmado de leishmaniosis visceral en la provincia de Misiones en el período 2009 al 2016. Para la identificación de Leishmania spp., los pacientes fueron sometidos a estudios diagnósticos indirectos (serológicos) y directos (microscopía, detección de ADN y secuenciación). También, se estudiaron variables como edad, sexo, lugar de residencia, y signos y síntomas clínicos indicativos de leishmaniosis visceral.Resultados. De los 24 pacientes estudiados, 18 (75 %) eran hombres y 6 (25 %) eran menores de cuatro años. La manifestación clínica más frecuente fue el síndrome febril prolongado en 21 (87,5 %) de los pacientes, seguido de esplenomegalia en 17 (70,8 %). Se identificó la especie Leishmania infantum en todos los pacientes estudiados.Conclusión. Este hallazgo constituye la primera identificación de la especie L. infantum en pacientes autóctonos de la provincia de Misiones. El estudio evidenció la importancia de la PCR para el manejo epidemiológico de la leishmaniosis visceral en Argentina.

A putative Leishmania panamensis/Leishmania braziliensis hybrid is a causative agent of human cutaneous leishmaniasis in Nicaragua

Parasitology ◽  
1994 ◽  
Vol 109 (4) ◽  
pp. 435-442 ◽  
Author(s):  
A. A. Belli ◽  
M. A. Miles ◽  
J. M. Kelly
Keyword(s):  
Strong Evidence ◽  
New World ◽  
Northern Region ◽  
Leishmania Braziliensis ◽  
Human Leishmaniasis ◽  
Leishmania Panamensis ◽  
Genotypic Characteristics ◽  
Epidemiological Significance ◽  
Human Cutaneous Leishmaniasis ◽  
Putative Hybrids

SUMMARYAs part of a survey of human leishmaniasis in Nicaragua we examined phenotypic and genotypic characteristics of 40 Leishmania isolates. We identified 3 distinct parasites associated with cutaneous disease in this area; Leishmania panamensis (40% of cases), Leishmania braziliensis (33%), and a strain which exhibits the heterozygous isoenzyme and DNA fingerprinting patterns expected of a L. panamensis/L. braziliensis hybrid (27%). There was complete correlation between the isoenzyme and DNA data for each of the putative hybrids examined. All of the ‘hybrids’ were obtained from foci in the northern region of the country where L. panamensis and L. braziliensis occur sympatrically. These observations provide strong evidence for sexual reproduction in New World Leishmania populations and suggest that it is of taxonomic and epidemiological significance.

scholarly journals Assessment of PCR in the detection of Leishmania spp in experimentally infected individual phlebotomine sandflies (Diptera: Psychodidae: Phlebotominae)

2002 ◽  
Vol 44 (5) ◽  
pp. 255-259 ◽  
Author(s):  
Érika M. MICHALSKY ◽  
Consuelo L. FORTES-DIAS ◽  
Paulo F.P. PIMENTA ◽  
Nágila F.C. SECUNDINO ◽  
Edelberto S. DIAS
Keyword(s):  
Digestive Tract ◽  
Infected Individual ◽  
Dna Amplification ◽  
Leishmania Braziliensis ◽  
Lutzomyia Longipalpis ◽  
Leishmania Chagasi ◽  
Phlebotomine Sandflies ◽  
Leishmania Spp ◽  
Polymerase Chain ◽  
Lutzomyia Migonei

DNA amplification by the polymerase chain reaction (PCR) was applied in the investigation of the presence of Leishmania (Kinetoplastida: Trypanosomatidae) parasites in single phlebotomine sandflies. Three phlebotomine/parasite pairs were used: Lutzomyia longipalpis/Leishmania chagasi, Lutzomyia migonei/Leishmania amazonensis and Lutzomyia migonei/Leishmania braziliensis, all of them incriminated in the transmission of visceral or cutaneous leishmaniasis. DNA extraction was performed with whole insects, with no need of previous digestive tract dissection or pooling specimens. The presence of either mouse blood in the digestive tract of the sandflies or the digestive tract itself did not interfere in the PCR. Infection by as few as 10 Leishmania sp. per individual were sufficient for DNA amplification with genus-specific primers. Using primers for L. braziliensis and L. mexicana complexes, respectively, it was possible to discriminate between L. braziliensis and L. amazonensis in experimentally infected vectors (L. migonei).

scholarly journals Comparative and systems analyses of Leishmania spp. non-coding RNAs through developmental stages

2021 ◽  
Author(s):  
J. Eduardo Martinez-Hernandez ◽  
Victor Aliaga-Tobar ◽  
Carolina Gonzalez ◽  
Rubens Monte-Neto ◽  
Alberto J. M. Martin ◽  
...  
Keyword(s):  
Leishmania Major ◽  
Developmental Stages ◽  
Genomic Structure ◽  
Neglected Diseases ◽  
Leishmania Braziliensis ◽  
Functional Relationships ◽  
Host Interaction ◽  
Leishmania Spp ◽  
Non Coding Rnas ◽  
And Function

Leishmania spp. is the causal agent of several diseases called leishmaniases, neglected diseases that seek to be eradicated in the coming years. We aimed to study the genomic structure and function of non-coding RNAs (ncRNAs) from Leishmania spp. and to get insights into its RNAome. We studied 26 strains corresponding to 16 different species of Leishmania genus. RNAome analysis revealed the presence of several ncRNAs that are shared through different species, allowing us to differentiate between subgenus and as well as species that are canonically related to visceral leishmaniasis. We found coexpression relationships within coding genes and ncRNAs, thus suggesting possible functional relationships between different coding genes-ncRNAs. Expression analysis in the metacyclic developmental stage comparison for Leishmania braziliensis and Leishmania major reveals the presence of shared coexpressed ncRNAs to several other coding genes in both species involved in chromatin structure and host interaction. This work constitutes the first effort to characterize the Leishmania RNAome, supporting further approaches to better understand the role of ncRNAs in the gene regulation, infective process and host-parasite interaction.

scholarly journals Major changes in chromosomal somy, gene expression and gene dosage driven by SbIII in Leishmania braziliensis and Leishmania panamensis

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Luz H. Patino ◽  
Hideo Imamura ◽  
Lissa Cruz-Saavedra ◽  
Paula Pavia ◽  
Carlos Muskus ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Dosage ◽  
Leishmania Braziliensis ◽  
Leishmania Panamensis

scholarly journals Combining diagnostic procedures for the management of leishmaniasis in areas with high prevalence of Leishmania guyanensis

2011 ◽  
Vol 86 (6) ◽  
pp. 1141-1144 ◽  
Author(s):  
Ednelza de Almeida Benicio ◽  
Ellen Pricilla Nunes Gadelha ◽  
Anette Talhari ◽  
Roberto Moreira da Silva Jr ◽  
Luis Carlos Ferreira ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Diagnostic Procedures ◽  
Diagnostic Methods ◽  
Species Discrimination ◽  
Leishmania Braziliensis ◽  
Hsp 70 ◽  
Chain Reaction ◽  
High Prevalence ◽  
Polymerase Chain ◽  
Leishmania Guyanensis

BACKGROUND: The Amazon region corresponds to approximately 40% of the cases of leishmaniasis in Brazil. We report a prospective study with 180 patients conducted in a health care unit that diagnoses 10% of the cases of leishmaniasis in the Brazilian Amazon. The study addresses how a combination of procedures improves diagnosis in areas with high prevalence of Leishmania guyanensis. OBJECTIVES: to evaluate diagnostic methods in areas with high prevalence of Leishmania guyanensis. METHODS: All subjects were amastigote-positive by direct microscopic examination of lesion scarifications. We conducted skin biopsy and histopathology, polymerase chain reaction and parasite cultivation. RESULTS: Polymerase chain reaction detected almost ninety percent of infections when two amplification protocols were used (mini-exon and HSP-70). HSP-70 specific polymerase chain reaction matched the sensitivity of parasite cultivation plus histopathology. CONCLUSION: The best combination was polymerase chain reaction plus histopathology, which increased diagnostic sensitivity to 94%. Species discrimination by polymerase chain reaction disclosed prevalence of human infections with Leishmania guyanensis of 94% and with Leishmania braziliensis of 6% for this region

Sign in / Sign up

Export Citation Format

Share Document