Diversity and function of culturable actinobacteria in the root-associated of Salvia miltiorrhiza Bunge

The root-associated actinobacteria play important roles in plant growth, nutrient use, and disease resistance due to their functional diversity. Salvia miltiorrhiza is a critical medicinal plant in China. The root actinobacterial community structure has been studied; however, the functions of root-associated actinobacteria of S. miltiorrhiza have not been elucidated. This study aimed to decipher the diversity and function of the culturable root-associated actinobacteria in plant growth using culture-dependent technology and culturable microbe metagenomes. We isolated 369 strains from the root-associated actinobacteria, belonging to four genera, among which Streptomyces was dominant. Besides, the functional prediction revealed some pathways related to plant growth, nitrogen and phosphorus metabolism, and antagonistic pathogens. We systematically described the diversity and functions of the culturable root-associated actinobacteria community. Our results demonstrated that the culturable root-associated actinobacteria of S. miltiorrhiza have rich functionalities, explaining the possible contribution of culturable root-associated actinobacteria to S. miltiorrhiza’s growth and development. This study provides new insights into understanding the function of the culturable root-associated actinobacteria and can be used as a knowledge base for plant growth promoters and biological control agent development in agriculture.

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Novel Insights into the Effect of Pythium Strains on Rapeseed Metabolism

Microorganisms ◽

10.3390/microorganisms8101472 ◽

2020 ◽

Vol 8 (10) ◽

pp. 1472

Author(s):

Kateřina Bělonožníková ◽

Kateřina Vaverová ◽

Tomáš Vaněk ◽

Miroslav Kolařík ◽

Veronika Hýsková ◽

...

Keyword(s):

Plant Growth ◽

Biological Control Agent ◽

Effective Strain ◽

Hydrolytic Enzymes ◽

Biochemical Properties ◽

Control Agent ◽

Growth Promoters ◽

Pythium Oligandrum ◽

Defense Compounds ◽

Plant Growth Promoters

Pythium oligandrum is a unique biological control agent. This soil oomycete not only acts as a mycoparasite, but also interacts with plant roots and stimulates plant defense response via specific elicitors. In addition, P. oligandrum can synthetize auxin precursors and stimulate plant growth. We analyzed the secretomes and biochemical properties of eleven Pythium isolates to find a novel and effective strain with advantageous features for plants. Our results showed that even closely related P. oligandrum isolates significantly differ in the content of compounds secreted into the medium, and that all strains secrete proteins, amino acids, tryptamine, phenolics, and hydrolytic enzymes capable of degrading cell walls (endo-β-1,3-glucanase, chitinase, and cellulase), exoglycosidases (especially β-glucosidase), proteases, and phosphatases. The most different strain was identified as a not yet described Pythium species. The changes in metabolism of Brassica napus plants grown from seeds coated with the tested Pythium spp. were characterized. Enhanced levels of jasmonates, ethylene precursor, and salicylic acid may indicate better resistance to a wide variety of pathogens. Glucosinolates, as defense compounds against insects and herbivores, were enhanced in young plants. Altogether, P. oligandrum strains varied in their life strategies, and either they could perform equally as plant growth promoters and mycoparasites or they had developed one of these strategies better.

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AKTIVITAS SIDEROFOR BACILLUS SUBTILIS SEBAGAI PEMACU PERTUMBUHAN DAN PENGENDALI PATOGEN TANAMAN TERUNG

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.217170-178 ◽

2017 ◽

Vol 17 (2) ◽

pp. 170 ◽

Cited By ~ 1

Author(s):

Nur Prihatiningsih ◽

Heru Adi Djatmiko ◽

Puji Lestari

Keyword(s):

Biological Control ◽

Bacillus Subtilis ◽

Plant Growth ◽

Biological Control Agent ◽

Control Agent ◽

Leaf Number ◽

Growth Promoters ◽

Root Volume ◽

Volume Weight ◽

Plant Growth Promoters

Siderophore activity of Bacillus subtilis as plant growth promoters and biological control agent of eggplants pathogens. The aims of this research were to identify the siderophores of B. subtilis, to assess its activities as plant growth promoters and biological control agent of eggplants pathogens. Five isolates of B. subtilis i.e.B46, B209, B211, B298 and B 315 grown on SDCASA medium. The isolate which showed the best siderophores production was then further studied on its ability as a growth promoter on eggplants in two soil types with different Fe content. The inhibitory test was conducted against two kinds of pathogens, namely Colletotrichum sp. and Ralstonia solanacearum. The greenhouse experiment was arranged using a factorial completely randomized block design. The first factor was the B. subtilis (B. subtilis B298 and without B. subtilis B298), second factor was the type of soil (Ultisol and Andisol). The variables measured were Fe uptake by plants, plant growth parameters on eggplant i.e. height, leaf number, root length, root volume, weight of fresh and dried shoot as well as fresh and dry root, percentage of inhibition to fungal and bacterial eggplant pathogens. The results showed that the five isolates of B. subtilis were able to produce siderophores as catecholate and hydroxamate types. The best siderophore production was showed by B. subtilis B298. The ability of B. subtilis B298 in accelerating the growth of plants was indicated by the increased of uptake Fe, plant height, leaf number, root volume, weight of dried plants by 45.62%, 25.48%, 19.45%, 41.10% and 34.89% respectively. The inhibition to the fungal and bacterial eggplant pathogens best shown by the isolates of B. subtilis B298 with 55.4% and 22 mm respectively.

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