scholarly journals Molecular characterization of clonal lineage and staphylococcal toxin genes fromS. aureusin Southern Nigeria

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5204 ◽  
Author(s):  
Funmilola A. Ayeni ◽  
Werner Ruppitsch ◽  
Franz Allerberger
Keyword(s):  
Protein A ◽  
High Potential ◽  
Clinical Samples ◽  
Staphylococcal Protein A ◽  
Clonal Lineage ◽  
Clinical Strains ◽  
Enterotoxin Genes ◽  
Southern Nigeria ◽  
Spa Types ◽  
Spa Type

BackgroundStaphylococcus aureusis a human colonizer with high potential for virulence, and the spread of the virulent strains from the colonized hosts to non-carriers in the community is on the increase. However, there are few reports on comprehensive analysis of staphylococcal enterotoxin (SE) genes with clonal lineage inS. aureusin Africa. This is essential because of diversity of cultures and habits of the people. This study analyzed spa types and enterotoxin genes inS. aureusstrains previously isolated from the human nostrils, poultry and clinical samples in Southern Nigeria.MethodsForty-sevenS. aureusisolates were obtained from humans nostrils (n = 13), clinical strains (n = 21) and poultry (n = 13) from previous studies in Southern Nigeria. The strains were analyzed formecAgene, selected toxins genes (sea, seb, sec, sed, see, seg, seh, sei, sej, sek, sel, sem, sen, seo, sep, seq, ser, seu)and Panton-Valentine leukocidin (PVL) gene(lukS-PV/lukF-PV)by PCR. Population structures of the strains were detected by Staphylococcal protein A (spa) typing.ResultsTwenty different spa types were obtained with the highest percentages, 17% observed inspatype t091 from clinical, nasal and poultry samples while t069 was the most prevalent spa type in poultry. Two MRSA were only detected in human strains. The poultry strains had the highest occurrence of SE genes (18%) followed by nasal strains (15%) and clinical strains (10%). Eighty-nine percent of all tested isolates harbored at least one SE gene;seowas the most prevalent (34%) followed byseg(30%) andsea(21%), whilesec, seeandsejwere absent in all strains. Spa type t355 was associated withlukS-PV/lukF-PVgene and complete absence of all studied SE.Sea, seq, seb, sekwere associated with spa type t069;seawas associated with t127 whilesepwas associated with spa type t091. There were coexistences ofseo/segandsei/seg.ConclusionsThe higher carriage of staphylococci enterotoxin genes by the nasal and poultryS. aureusstrains suggests a high potential of spread of staphylococcal food poisoning through poultry and healthy carriers in the community. This is the first report of high occurrence of staphylococcal enterotoxins genes in poultry from Nigeria.

scholarly journals Molecular characterization of clonal lineage and Staphylococcal toxin genes from S. aureus in Southern Nigeria

2018 ◽  
Author(s):  
Funmilola Ayeni ◽  
Werner Ruppitsch ◽  
Franz Allerberger
Keyword(s):  
Protein A ◽  
Food Poisoning ◽  
Staphylococcal Protein A ◽  
Toxin Genes ◽  
Serious Infections ◽  
Southern Nigeria ◽  
Population Structures ◽  
Toxic Shock Syndrome Toxin ◽  
Spa Types ◽  
Spa Type

Background. The pathogenic role of S. aureus as causative agent of serious infections and food poisoning is on the increase. However, there are few reports on comprehensive analyses of toxins and staphylococcal enterotoxin (SE) genes in S. aureus in Africa. This study analyzed spa types and toxin genes in S. aureus obtained from our previous studies in Southern Nigeria. Methods. Forty-seven non-duplicate S. aureus isolates were obtained from humans (n = 34) and poultry (n = 13) from previous studies in Southern Nigeria. The strains were analyzed for mecA, selected toxins genes (sea, seb, sec, sed, see, seg, seh, sei, sej, sek, sel, sem, sen, seo, sep, seq, ser, seu), TSST and lukS-PV/lukF-PV by PCR. Population structures of the strains were detected by Staphylococcal protein A (spa) typing. Results. Twenty three percent of all isolates (47) carried the Panton-Valentine leukocidin (PVl) gene. Two MRSA were detected. Twenty different spa types were obtained, with the highest percentages, 17% belonging to spa type t091 was observed in 4 states from clinical, nasal and poultry samples while t069 is the most prevalent type in poultry. Eighty-nine percent of the all tested isolates harbored at least one staphylococcus enterotoxin. Seo was the most prevalent SE (34%) followed by seg (30%) and sea (21%), while toxic shock syndrome toxin (TSST), seb,sec,,see, sej, sel, sem, and ser, seu were absent in all strains. Spa type t355 was associated with the PVl and complete absence of all studied SE. Sea, seq, seb, sek were associated with spa type 069; t127 was associated with sea while sep was associated with spa type t091. There was coexistence of seo/seg and sei/seg. Conclusions. We detected a high incidence of enterotoxins and PVl encoding genes in these potential staphylococcal reservoir. Specific toxin genes were observed in particular spa types.

scholarly journals Molecular characterization of clonal lineage and Staphylococcal toxin genes from S. aureus in Southern Nigeria

2018 ◽  
Author(s):  
Funmilola Ayeni ◽  
Werner Ruppitsch ◽  
Franz Allerberger
Keyword(s):  
Protein A ◽  
Food Poisoning ◽  
Staphylococcal Protein A ◽  
Toxin Genes ◽  
Serious Infections ◽  
Southern Nigeria ◽  
Population Structures ◽  
Toxic Shock Syndrome Toxin ◽  
Spa Types ◽  
Spa Type

Background. The pathogenic role of S. aureus as causative agent of serious infections and food poisoning is on the increase. However, there are few reports on comprehensive analyses of toxins and staphylococcal enterotoxin (SE) genes in S. aureus in Africa. This study analyzed spa types and toxin genes in S. aureus obtained from our previous studies in Southern Nigeria. Methods. Forty-seven non-duplicate S. aureus isolates were obtained from humans (n = 34) and poultry (n = 13) from previous studies in Southern Nigeria. The strains were analyzed for mecA, selected toxins genes (sea, seb, sec, sed, see, seg, seh, sei, sej, sek, sel, sem, sen, seo, sep, seq, ser, seu), TSST and lukS-PV/lukF-PV by PCR. Population structures of the strains were detected by Staphylococcal protein A (spa) typing. Results. Twenty three percent of all isolates (47) carried the Panton-Valentine leukocidin (PVl) gene. Two MRSA were detected. Twenty different spa types were obtained, with the highest percentages, 17% belonging to spa type t091 was observed in 4 states from clinical, nasal and poultry samples while t069 is the most prevalent type in poultry. Eighty-nine percent of the all tested isolates harbored at least one staphylococcus enterotoxin. Seo was the most prevalent SE (34%) followed by seg (30%) and sea (21%), while toxic shock syndrome toxin (TSST), seb,sec,,see, sej, sel, sem, and ser, seu were absent in all strains. Spa type t355 was associated with the PVl and complete absence of all studied SE. Sea, seq, seb, sek were associated with spa type 069; t127 was associated with sea while sep was associated with spa type t091. There was coexistence of seo/seg and sei/seg. Conclusions. We detected a high incidence of enterotoxins and PVl encoding genes in these potential staphylococcal reservoir. Specific toxin genes were observed in particular spa types.

scholarly journals 1168. Are the Staphylococcus aureus Isolates that Recolonize Infants in a NICU Following Successful Decolonization the Same or Different from the Initial Colonizing Isolate?

2021 ◽  
Vol 8 (Supplement_1) ◽  
pp. S675-S676
Author(s):  
Lorry G Rubin ◽  
Archana Balamohan ◽  
Angela Gomez-Simmonds ◽  
Vitaliya Boyar ◽  
Alexander Chong ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Phylogenetic Analyses ◽  
Protein A ◽  
Illumina Miseq ◽  
Original Strain ◽  
Staphylococcal Protein A ◽  
Spa Typing ◽  
Surveillance Cultures ◽  
Spa Types ◽  
Spa Type

Abstract Background Staphylococcus aureus is an important pathogen of infants in a neonatal intensive care unit (NICU). Colonization precedes infection and decolonization may prevent infection. The origin of colonizing organisms may be the NICU environment or personnel or visitors. We have observed infants who became recolonized after successful decolonization. The purpose of this study was to determine the proportion of infants who become recolonized with the same strain or a different strain. Methods Eligible infants were consecutive infants who 1. were colonized with methicillin-susceptible S. aureus and were successfully decolonized with topical mupirocin ointment (nares and umbilicus) as evidenced by 2 or more consecutive negative weekly surveillance cultures (in the absence of a course of systemic antibiotics with activity against MSSA), 2. subsequently became recolonized, and 3. the pair of isolates was available for analysis. Isolates were analyzed by staphylococcal protein A (spa) typing and pairs with concordant spa types were subjected to whole genome sequencing (WGS; Illumina MiSeq) and phylogenetic analyses. Pairs of isolates with fewer than 25 single nucleotide polymorphism differences were considered closely related. Results There were 19 occurrences of MSSA recolonization in 17 infants following 2-6 (median, 2) negative weekly intervening surveillance cultures. Based upon spa typing (that identified 19 spa types), in 11 (58%) there was a concordant spa type and in 8 (42%) there was a discordant spa type. Of the 11 pairs of isolates with concordant spa types that were compared after WGS, 10 were closely related resulting overall in recolonization with a closely related strain in 53% of episodes. Conclusion Among MSSA colonized infants who become recolonized after successful decolonization, the recolonizing strain is the same as the original strain in over half of cases. In such cases the source is more likely to be a visitor than the NICU environment or staff. The possibility that some cases classified as recolonization were in fact persistent low level colonization or carriage in another body site not detected by surveillance cultures cannot be excluded. Disclosures Anne-Catrin Uhlemann, MD, PhD, Merck (Grant/Research Support)

scholarly journals The molecular epidemiology of Staphylococcus aureus of bovine mastitis origin

2021 ◽  
Vol 91 (1) ◽  
pp. 1-10
Author(s):  
Tawheed Ahmad Shafi ◽  
◽  
Baljinder K. Bansal ◽  
Shukriti Sharma ◽  
Dhiraj K. Gupta ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Epidemiology ◽  
Protein A ◽  
Bovine Mastitis ◽  
Subclinical Mastitis ◽  
Staphylococcal Protein A ◽  
Spa Typing ◽  
New Novel ◽  
Spa Types ◽  
High Diversity

The present study was aimed to understand the molecular epidemiology of Staphylococcus aureus (54 isolates), isolated from 422 milk samples obtained from 108 subclinical mastitis affected cows (CMT positive ≥1+ in at least one quarter). The molecular epidemiology of Staphylococcus aureus was studied using coagulase (coa) gene polymorphism, 16S-23S ribosomal spascer (RS-PCR) polymorphism and Staphylococcal protein A (Spa) typing. Staphylococcus aureus produced 7 coagulase genotypes and 8 RS genotypes respectively. Coagulase genotype GTIII (730 bp) was the most prevalent (35 strains) followed by GTV (900 bp, 7 strains) and GTIV (800 bp, 4 strains), whereas RS genotypes GTA accounted for the highest number of strains (31 strains), followed by GTB (11strains), GTH (4 strains) and GTE (3 strains). Coagulase genotype CTIII (730 bp) showed the highest diversity, as isolates within it produced 5 RS genotypes, the majority of them belonging to the RS genotype GTA (29 out of 31 strains). Forty out of 54 Staphylococcus aureus samples isolated in this study were correctly typed by spa typing, and were assigned to 21 known spa types, and one new novel spa type t18462. The study revealed high diversity within Staphylococcus aureus strains, consisting of 7 coagulase genotypes, 8 RS genotypes and 22 spa types.

scholarly journals 233. The Epidemiology, Genomics, and Evolution of Staphylococcus aureus in Northeast Ohio

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S133-S134
Author(s):  
Richard R Watkins ◽  
Dipendra Thapaliya ◽  
Rami Savri ◽  
Tara Smith
Keyword(s):  
Protein A ◽  
Meca Gene ◽  
Cleveland Clinic ◽  
Clinical Samples ◽  
Effective Prevention ◽  
Institutional Review ◽  
Effective Prevention Strategies ◽  
Spa Types ◽  
Discriminatory Index ◽  
Panton Valentine Leukocidin

Abstract Background Infections due to S. aureus result in significant morbidity, mortality, and healthcare expense. We sought to identify the strains of S. aureus causing infections in hospitalized patients in Northeast Ohio and determine whether they are reflective of the S. aureus strains present in the surrounding environment. Methods The study was approved by the Institutional Review Board at Cleveland Clinic Akron General. Clinical S. aureus isolates (n = 300) were cultured and PCR was used to amplify the staphylococcus protein A (spa), Panton–Valentine Leukocidin (PVL), and mecA genes. The clinical spa types were compared with ones from our data base of S. aureus strains previously collected and sequenced from the community and environment in Northeast Ohio. Results A total of 51 spa types were detected from 129 S. aureus clinical isolates (discriminatory index, 0.876; 95% confidence interval [CI], 0.827–0.925; Table 1). The most common spa types were t008 (42/129, 32.6%), t002 (16/129, 12.4%), and t334 (6/129, 4.7%). In comparison, the most frequently detected spa types from the environmental samples were t189 (40/257, 15.6%), t002 (16/257, 6.2%), and t008 (11/257, 4.3%). Among the S. aureus isolates (n = 146), 45 were PVL-positive (30.8%) and 94 (66.7%) carried mecA. Of the 42 t008 (ST8/USA300; a common community-associated strain) isolates, 35 (83.3%) were methicillin-resistant S. aureus (MRSA) (based on the presence of the mecA gene) and 25 (59.5%) were PVL-positive. Thirteen of the sixteen (81.2%) t002 (ST5/USA100; a common hospital-associated strain) were MRSA and only one (6.2%) was PVL-positive. Conclusion There is considerable overlap of S. aureus strains present in clinical samples with those found in the environment. This finding should draw attention to the need for more effective prevention strategies to reduce the risk of transmission of S. aureus, including MRSA, in the environment to humans. Disclosures All authors: No reported disclosures.

scholarly journals Novel spa and Multi-Locus Sequence Types (MLST) of Staphylococcus Aureus Samples Isolated from Clinical Specimens in Korean

Antibiotics ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 202 ◽  
Author(s):  
Yae Sung Mun ◽  
You Jin Hwang
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Bacterial Infections ◽  
Protein A ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Methicillin Resistant ◽  
Sequence Types ◽  
Spa Types ◽  
Spa Type

Staphylococcus aureus bacteremia is one of the most frequent and severe bacterial infections worldwide. The increased incidence of S. aureus infections with a diverse pattern of S. aureus protein A (spa) types across different geographic regions is a global challenge. This study investigated a novel spa type of methicillin-resistant S. aureus in a clinically isolated specimen. A total of 109 clinical S. aureus samples were subjected to 19 sets of antimicrobial susceptibility testing using the Kirby–Bauer disk diffusion method. Molecular typing was performed with S. aureus protein A (spa) and multi-locus sequence types (MLST) via polymerase chain reaction and sequencing. The methicillin-resistant S. aureus samples in our study accounted for 55.05% (60/109) of the total. A novel spa type was detected in five (5/60) strains. This gh22 isolate was identified in antimicrobial susceptibility tests of 15 kinds of antibiotics. Antibiotic resistance genes included mecA, TEM, aac(6′)-aph(2”), ermA, and tetM. Eleven S. aureus samples were classified as t2460, t338, t324, t693, five unknown spa types (new spa types), and undefined MLST (novel MLST). We report a high prevalence rate of t2460 methicillin-resistant S. aureus samples in our country. Additionally, novel spa gh22, MLST ST4613, and clonal compact CC5-type strains (T1:M1:B1:B1:M1:E1:K1, r26:r17:r34:r34:r17:r13:r16, mlst;1:4:1:4:559:495:10) showing multidrug resistance were identified among S. aureus samples.

scholarly journals 1164. Acquisition of Methicillin-Susceptible Staphylococcus aureus in the Neonatal Intensive Care Unit: Molecular Comparison of Relatedness of Isolates

2021 ◽  
Vol 8 (Supplement_1) ◽  
pp. S673-S674
Author(s):  
Dalia F Eid ◽  
Angela Gomez-Simmonds ◽  
Vitaliya Boyar ◽  
Anne-Catrin Uhlemann ◽  
Lorry G Rubin
Keyword(s):  
Neonatal Intensive Care ◽  
Protein A ◽  
Illumina Miseq ◽  
Healthcare Personnel ◽  
Control Group ◽  
Staphylococcal Protein A ◽  
Spa Typing ◽  
Exact Test ◽  
Molecular Comparison ◽  
Spa Type

Abstract Background In the NICU MSSA infections occur frequently and cause morbidity and mortality. Colonization is a risk factor for infection. Optimal infection prevention strategies await a more complete understanding of acquisition and transmission. To investigate possible transmission, we studied whether newly MSSA colonized infants share a strain with another contemporaneously colonized infant. Methods This is a prospective observational study in a level IV NICU from April through November 2019. Infants had weekly MSSA nasal surveillance cultures. Isolates from newly MSSA colonized infants and other infants colonized with MSSA during the same/previous week were subjected to staphylococcal protein A (spa) typing; most pairs with a concordant (CC) spa type were analyzed by whole genome sequencing (WGS; Illumina MiSeq). Pairs of isolates with a CC spa type and < 25 single nucleotide polymorphism differences on WGS were considered closely related (CC pairs). A control group consisted of pairs of isolates from a newly colonized infant with one randomly chosen colonized infant with a discordant (DC) spa type during the same/previous week. The medical records were reviewed for staff member (SM) and room assignment. Fischer’s exact test was used to compare proportions. Results Isolates from 60/68 consecutive newly MSSA colonized infants and 111/133 comparison infants were available for spa typing. Of these 60 infants, 23 (38 %) had a CC spa type with another infant colonized during the same/previous week. Of 18 isolate pairs from infants with a CC spa type that were subjected to WGS, 12 (67%) pairs of isolates were closely related. 7/12 (58 %) of CC pairs had a SM in common compared to 2/13 (15 %) in the DC pair groups, p=0.04. 2/12 (17 %) of CC pairs shared a room compared to 2/13 (15 %) pairs in the DC group, p=1.0. Conclusion Among newly MSSA colonized infants at least 25% are colonized with an isolate closely related to that of another colonized infant indicating likely infant to infant transmission. WGS is more discriminatory than spa typing for MSSA. Given the lack of commonality of room assignment and the commonality of SM assignment, a possible role of healthcare personnel in MSSA transmission should be further investigated. Disclosures Anne-Catrin Uhlemann, MD, PhD, Merck (Grant/Research Support)

scholarly journals 1217. Staphylococcus Protein A (spa) Typing Demonstrates Genetic Heterogeneity of Methicillin-Susceptible Staphyloccus aureus (MSSA) in a Neonatal Intensive Care Unit (NICU)

2018 ◽  
Vol 5 (suppl_1) ◽  
pp. S369-S369
Author(s):  
Alexandra Hill-Ricciuti ◽  
Samantha Ferguson ◽  
Wenjing Geng ◽  
Stephania Stump ◽  
Maria Messina ◽  
...  
Keyword(s):  
Genetic Heterogeneity ◽  
Neonatal Intensive Care ◽  
Local Community ◽  
Protein A ◽  
Infection Prevention And Control ◽  
Multiple Sources ◽  
Spa Typing ◽  
Incident Cases ◽  
Spa Types ◽  
Spa Type

Abstract Background In the NICU, MSSA is a more prevalent pathogen than MRSA, but optimal infection prevention and control strategies for MSSA are not yet well understood. There are likely multiple routes of MSSA acquisition given its role as normal flora and its detection in the anovaginal tract of pregnant women. We describe the molecular epidemiology of MSSA in our NICU during a yearlong surveillance effort. Methods Included infants were hospitalized in a university-affiliated level III-IV NICU from January to December 2017 (1032 admissions) and had positive clinical and/or surveillance cultures for MSSA. Infants admitted at ≥7 days of age were screened for MSSA colonization by culturing the anterior nares and three skin sites. All infants in the NICU were screened twice monthly. Spa typing was performed to genetically characterize isolates. Results During the study period, MSSA was identified in 187 infants (18 at admission, 145 by twice monthly surveillance, and 24 from clinical cultures). In all, 269 MSSA isolates (245 surveillance and 24 clinical isolates) from 166 infants were spa typed. Sixty-two MSSA spa types were identified; 31 (50%) were each detected in only one infant. The incidence of the nine most common spa types is shown (Figure 1); t279 (13%), t002 (8%), and t1451 (6%) had the highest incidence. t1451 and t571 belong to ST398, a common MSSA clone in the local community. The epidemiology of spa types varied; e.g., incident cases of t279 was detected in 10 months, t1451 was detected in 6 months and t148 in 3 months. Among the 14 sets of twins and triplets with MSSA isolates, 12 (86%) shared the same spa type as their sibling(s). Of the 58 infants with >1 MSSA isolate, 12 (21%) acquired new spa-types. No spa type(s) predominated in the 19 episodes of invasive infections. In 6 infants with both colonizing and invasive isolates, colonizing and invasive isolates were the same spa type(s) in 5. Conclusion Spa typing demonstrated that MSSA isolates in our NICU exhibited substantial genetic heterogeneity. While these data do not elucidate acquisition route(s), they suggest infants are acquiring MSSA from multiple sources, likely including family members and the local community. Ongoing sequencing studies are examining common spa types to further understand transmission dynamics. Disclosures A. C. Uhlemann, Merck: Investigator, Grant recipient

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