scholarly journals Morpho-histological development of the somatic embryos of Typha domingensis

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5952 ◽  
Author(s):  
Guadalupe Hernández-Piedra ◽  
Violeta Ruiz-Carrera ◽  
Alberto J. Sánchez ◽  
Arlette Hernández-Franyutti ◽  
Alfonso Azpeitia-Morales
Keyword(s):  
Somatic Embryogenesis ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Typha Domingensis ◽  
Light Conditions ◽  
Maturation Phase ◽  
Auxin Concentration ◽  
Embryogenic Line ◽  
Dark Conditions ◽  
Embryogenic Lines

Background Sustainable methods of propagation of Typha domingensis through somatic embryogenesis can help mitigate its current condition of ecological marginalization and overexploitation. This study examined whether differentiation up to coleoptilar embryos could be obtained in an embryogenic line proliferated with light and high auxin concentration. Methods Murashige and Skoog medium at half ionic strength and containing 3% sucrose and 0.1% ascorbic acid was used for the three embryogenic phases. Induction started with aseptic 9-day-old germinated seeds cultured in 0.5 mg L−1 2,4-dichlorophenoxyacetic (2,4-D). Proliferation of the embryogenic callus was evaluated at 2,4-D concentrations ranging from 0 to 2 mg L−1 in cultures maintained in the dark. The dominant embryogenic products obtained in each treatment were used as embryogenic lines in the third phase. Thus, maturation of the somatic embryos (SEs) was analyzed using four embryogenic lines and under light vs. dark conditions. Embryogenic differentiation was also monitored histologically. Results Proliferation of the nine morphogenetic products was greater in the presence of 2,4-D, regardless of the concentration, than in the absence of auxin. Among the products, a yellow callus was invariably associated with the presence of an oblong SE and suspended cells in the 2,4-D treatments, and a brown callus with scutellar somatic embryos (scSEs) in the treatment without 2,4-D. During the maturation phase, especially the embryogenic line but also the light condition resulted in significant differences, with the highest averages of the nine morphogenetic products obtained under light conditions and the maximum concentration of auxin (YC3 embryogenic line). Only this line achieved scSE growth, under both light and dark conditions. Structurally complete coleoptilar somatic embryos (colSEs) could be anatomically confirmed only during the maturation phase. Discussion In the embryogenic line cultured with the highest auxin concentration, light exposure favored the transdifferentiation from embryogenic callus to scSE or colSE, although growth was asynchronous with respect to the three embryogenic phases. The differentiation and cellular organization of the embryos were compatible with all stages of embryogenic development in other monocotyledons. The growth of colSEs under light conditions in the YC3 embryogenic line and the structurally complete anatomic description of colSEs demonstrated that differentiation up to coleoptilar embryos could be obtained. The diversity of embryogenic products obtained in the YC3 embryogenic line opens up the opportunity to synchronize histological descriptions with the molecules associated with the somatic embryogenesis of Typha spp.

scholarly journals Morphohistological development of the somatic embryo of Typha domingensis

2018 ◽  
Author(s):  
Guadalupe Hernández-Piedra ◽  
Violeta Ruiz-Carrera ◽  
Alberto J Sánchez ◽  
Arlette Hernández-Franyutti ◽  
Alfonso Azpeitia-Morales
Keyword(s):  
Somatic Embryo ◽  
Somatic Embryos ◽  
Histological Analysis ◽  
Typha Domingensis ◽  
Cellular Organization ◽  
Light Conditions ◽  
Embryogenic Calluses ◽  
Embryogenic Line ◽  
Dark Conditions ◽  
Suspended Cells

Background. The sustainable methods of propagation for Typha domingensis through somatic embryogenesis can help to mitigate its current condition of ecological marginalisation and overexploitation. Then, the hypothesis established that the variation of the concentration of auxin and light conditions in sequential stages of culture generate different morphogenetic routes that can be monitoring by morphohistological markers. Methods. Murashige and Skoog medium at half ionic strength, 3% sucrose and 0.1% ascorbic acid were used in the induction, proliferation and embryogenic maturation. Induction started with aseptic germinates cultured in 0.5 mg L-1 of 2,4-dichlorophenoxyacetic. Four concentrations of 0 to 2 mg L-1 of 2,4-dichlorophenoxyacetic, that generated four embryogenic lines, were evaluated in darkness. Maturation of the somatic embryo took place, in each embryogenic line, without auxin and under light and dark conditions. Results. The yellow and brown callus, as well as oblong and scutellar somatic embryos were recorded in the methodological sequence. The embryogenic differentiation was described with histological analysis. The induced cultures produced both somatic embryos in a small proportion. The percentages of the yellow callus on the explant and of suspended cells in the embryogenic proliferation were greater with the three concentrations of 2,4-dichlorophenoxyacetic. While, the brown callus predominated without auxin. The somatic embryo developed under light and dark conditions, and presented globular, oblong, scutellar and sparsely coleoptilar stages. Discussion. The combined effect of auxin concentrations and light-dark conditions generated conditions that favoured the development of embryogenic calluses and somatic embryos (globular, oblong, scutellar and coleoptilar) in an asynchronous process with respect to the stages of embryogenic induction, proliferation, and maturation. Indeed, differentiation and cellular organization of this process were compatible with descriptors of the embryogenic stages recorded by other aquatic and terrestrial monocotyledons.

scholarly journals Morphohistological development of the somatic embryo of Typha domingensis

2018 ◽  
Author(s):  
Guadalupe Hernández-Piedra ◽  
Violeta Ruiz-Carrera ◽  
Alberto J Sánchez ◽  
Arlette Hernández-Franyutti ◽  
Alfonso Azpeitia-Morales
Keyword(s):  
Somatic Embryo ◽  
Somatic Embryos ◽  
Histological Analysis ◽  
Typha Domingensis ◽  
Cellular Organization ◽  
Light Conditions ◽  
Embryogenic Calluses ◽  
Embryogenic Line ◽  
Dark Conditions ◽  
Suspended Cells

Background. The sustainable methods of propagation for Typha domingensis through somatic embryogenesis can help to mitigate its current condition of ecological marginalisation and overexploitation. Then, the hypothesis established that the variation of the concentration of auxin and light conditions in sequential stages of culture generate different morphogenetic routes that can be monitoring by morphohistological markers. Methods. Murashige and Skoog medium at half ionic strength, 3% sucrose and 0.1% ascorbic acid were used in the induction, proliferation and embryogenic maturation. Induction started with aseptic germinates cultured in 0.5 mg L-1 of 2,4-dichlorophenoxyacetic. Four concentrations of 0 to 2 mg L-1 of 2,4-dichlorophenoxyacetic, that generated four embryogenic lines, were evaluated in darkness. Maturation of the somatic embryo took place, in each embryogenic line, without auxin and under light and dark conditions. Results. The yellow and brown callus, as well as oblong and scutellar somatic embryos were recorded in the methodological sequence. The embryogenic differentiation was described with histological analysis. The induced cultures produced both somatic embryos in a small proportion. The percentages of the yellow callus on the explant and of suspended cells in the embryogenic proliferation were greater with the three concentrations of 2,4-dichlorophenoxyacetic. While, the brown callus predominated without auxin. The somatic embryo developed under light and dark conditions, and presented globular, oblong, scutellar and sparsely coleoptilar stages. Discussion. The combined effect of auxin concentrations and light-dark conditions generated conditions that favoured the development of embryogenic calluses and somatic embryos (globular, oblong, scutellar and coleoptilar) in an asynchronous process with respect to the stages of embryogenic induction, proliferation, and maturation. Indeed, differentiation and cellular organization of this process were compatible with descriptors of the embryogenic stages recorded by other aquatic and terrestrial monocotyledons.

scholarly journals Effect of Cryopreservation on Olive (Olea europaea L.) Plant Regeneration via Somatic Embryogenesis

Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 34
Author(s):  
Fatiha Bradaï ◽  
Carolina Sánchez-Romero
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Aluminum Foil ◽  
Regeneration Capacity ◽  
Fresh Weight ◽  
Negative Effects ◽  
Embryogenic Cultures ◽  
Embryogenic Line ◽  
Cryopreservation Protocol ◽  
Embryogenic Lines

Olive somatic embryos have been successfully cryopreserved using the droplet-vitrification method on aluminum foil strips. Although acceptable recovery rates have been obtained after rewarming, the influence of this cryopreservation protocol on the somatic embryogenesis process is unknown. To evaluate the effect of cryopreservation on olive somatic embryogenesis, the behavior of cultures established from cryopreserved somatic embryos was compared with that of control, non-cryopreserved cultures in the different phases of the somatic embryogenesis process. In order to analyze the influence of the genotype, this investigation was carried out in two independent lines. During the proliferation step, only the line T1 was affected by cryopreservation, with higher fresh weight increases. Although similar total embryos were produced per culture, freezing in liquid nitrogen significantly improved the maturation pattern in the line P5. Better germination results were also found in this embryogenic line. The genotype plays a key role, largely determining the effect of cryopreservation on olive somatic embryogenesis. A specific genotype-dependent response was found depending on the culture step. Variations observed could not be associated to differences in the embryogenic lines’ instability to maintain their morphogenic competence after cryopreservation. Embryogenic cultures established after rewarming retained their regeneration capacity, with no evident negative effects affecting their regeneration capacity.

scholarly journals Plant Regeneration via Somatic Embryogenesis from Leaf and Floral Explants of ‘Chancellor’ Wine Grape

1970 ◽  
Vol 20 (2) ◽  
pp. 157-170 ◽  
Author(s):  
Richard M.S. Mulwa ◽  
Margaret M.A. Norton ◽  
Robert M. Skirvin
Keyword(s):  
Somatic Embryogenesis ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Basal Medium ◽  
Limiting Factor ◽  
Half Strength ◽  
Conversion Stage ◽  
Cotyledon Expansion ◽  
Floral Explants ◽  
Shoot Meristems

Abundant embryogenic callus was obtained from leaf and floral explants of "Chancellor" grape by continuous culture for 12 weeks on Nitsch and Nitsch basal medium supplemented with 9 μM 2, 4-D + 17 μM IASP + either 1 μM BA or 1 μM TDZ (ECIM) in darkness. They were successfully maintained by a five to six week subculture interval on NN medium containing 2 μM 2, 4-D + 0.2 μM TDZ + 4 μM IASP (LTMM). Near synchronous embryo developed from embryogenic callus on medium containing 10 μM IASP + 8 μM NOA + 1 μM TDZ + 1 μM ABA + 2.5 g/l AC (EDMM).  Individually separated somatic embryos were germinated on both NN and half strength of MS containing 0.5 μM BA + 0.025 μM NAA, respectively; normal plantlet conversion from embryos was low (35%).  Whole fruiting plants were obtained. Aberrant embryo development was characterized by failure to form functional shoot meristems following the initial cotyledon expansion during germination. These observations indicate that the embryo conversion stage of the regeneration is difficult and remains a limiting factor requiring more empirical experimentation for improvement in grape tissue culture.   Key words: Chancellor grape, Regeneration, Somatic embryogenesis   D.O.I. 10.3329/ptcb.v20i2.6895   Plant Tissue Cult. & Biotech. 20(2): 157-170, 2010 (December)

scholarly journals EFFECT OF DIFFERENT SOURCES OF PLANT GROWTH REGULATOR ON THE INDUCTION AND DEVELOPMENT OF MANGOSTEEN SOMATIC EMBRYOS

2017 ◽  
Vol 17 (1) ◽  
pp. 9
Author(s):  
Yosi Zendra Joni ◽  
Riry Prihatini ◽  
Darda Efendi ◽  
Ika Roostika
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Growth ◽  
Embryogenic Callus ◽  
Plant Growth Regulator ◽  
Somatic Embryos ◽  
Casein Hydrolysate ◽  
Malt Extract ◽  
Mass Propagation ◽  
Embryogenic Callus Induction

<p>Somatic embryogenesis is a technique for regenerating embryos derived from somatic cells of various plant species. This technique along with the utilization of plant growth regulator (PGR) might benefit for mass propagation and improvement of plant species through biotechnological tools. The study aimed to determine the effect of different plant growth regu-lators, namely 6-benzyladenine (BA) and thidiazuron (TDZ) on the embryogenic callus induction as well as casein hydrolysate and malt extract on the somatic embryo development of mangosteen. The explants used were in vitro young stems of mangosteen clone Leuwiliang. This study consisted of two experiments, namely induction of embryogenic callus and formation of somatic embryo. The first experiment was arranged as factorial in a completely randomized design with BA (0 and 0.7 mg l-1) as the first factor and TDZ (0, 0.1, 0.5 and 1.0 mg l-1) as the second factor. The second experiment consisted of four treatments, i.e. casein hydrolysate and malt extract at the rate of 500 and 1,000 mg l-1. The results showed that the best medium for embryogenic callus induction was MS supplemented with 0.1 mg l-1 TDZ, which resulted semifriable calli. Casein hydrolysate and malt extract could not induce the formation of somatic embryos. After two times subcultures on the same MS medium supplemented with 0.5 mg l-1 TDZ and 0.7 mg l-1 BA, a total of 33.8 somatic embryos per explant was induced. The successful somatic embryogenesis would support mangosteen breeding and in vitro mass propagation program.</p>

scholarly journals Can Ethylene Inhibitors Enhance the Success of Olive Somatic Embryogenesis?

Plants ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 168
Author(s):  
Muhammad Ajmal Bashir ◽  
Cristian Silvestri ◽  
Amelia Salimonti ◽  
Eddo Rugini ◽  
Valerio Cristofori ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Salicylic Acid ◽  
Silver Nitrate ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Cobalt Chloride ◽  
Zygotic Embryos ◽  
Ethylene Inhibitors ◽  
Embryogenic Calli ◽  
Stimulatory Action

An efficient in vitro morphogenesis, specifically through somatic embryogenesis, is considered to be a crucial step for the application of modern biotechnological tools for genetic improvement in olive (Olea europaea L.). The effects of different ethylene inhibitors, i.e., cobalt chloride (CoCl2), salicylic acid (SA), and silver nitrate (AgNO3), were reported in the cyclic somatic embryogenesis of olive. Embryogenic callus derived from the olive immature zygotic embryos of the cultivar Leccino, was transferred to the expression ECO medium, supplemented with the ethylene inhibitors at 20 and 40 µM concentrations. Among these, the maximum number of somatic embryos (18.6) was obtained in media containing silver nitrate (40 µM), followed by cobalt chloride (12.2 somatic embryos @ 40 µM) and salicylic acid (40 µM), which produced 8.5 somatic embryos. These compounds interfered on callus traits: white friable embryogenic calli were formed in a medium supplemented with 40 µM cobalt chloride and salicylic acid; in addition, a yellow-compact embryogenic callus appeared at 20 µM of all the tested ethylene inhibitors. The resulting stimulatory action of silver nitrate among all the tested ethylene inhibitors on somatic embryogenesis, clearly demonstrates that our approach can efficiently contribute to the improvement of the current SE protocols for olive.

scholarly journals Bioproduction of Diosgenin in Callus Cultures of Balanites aegyptiaca: Effect of Growth Regulators, Explants and Somatic Embryogenesis

2006 ◽  
Vol 1 (3) ◽  
pp. 1934578X0600100
Author(s):  
Bishnu P. Chapagain ◽  
Vinod Saharan ◽  
Dan Pelah ◽  
Ram C. Yadav ◽  
Zeev Wiesman
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Callus Formation ◽  
Basal Medium ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Balanites Aegyptiaca ◽  
Basal Media

This study describes the effects of plant growth regulators, explants, and somatic embryogenesis on in vitro production of the steroidal sapogenin, diosgenin, in callus cultures of the Balanites aegyptiaca (L.) Del.(desert date). Root, shoot, hypocotyl, and epicotyl callus culture of B. aegyptiaca, were raised on MS basal media supplemented with various combinations of either 2,4-D and NAA alone, or with BAP. The diosgenin content (on a dry weight basis) was found to be highest when calli were cultured in MS basal medium supplemented with 1.0 mg l−1 2,4-D alone and/or in combination with 0.5 mg l−1 BAP. However, the callus growth was highest in media supplemented with 2.5 or 3.0 mg l−1 2,4-D. MS basal media supplemented with 2,4-D 2.5 mg l−1 alone and in combination with 0.5 mg l−1 BAP induced pre-embryogenic callus formation on root cultures. When these pre-embryogenic callus cultures were used to establish cell suspension cultures, two growth densities were obtained in embryogenic suspension cultures, inducing clusters of somatic embryos at various stages of development. The maximum number of somatic embryos were obtained at the fifth week on the medium supplemented with 1.0 mg l−1 2,4-D. However, the diosgenin content in these somatic cells was found to be lower compared to the explant calluses. This study revealed that production of diosgenin in callus cultures of B. aegyptiaca is possible, but the amount is significantly affected by the growth regulators, type of explants, and somatic embryogenesis.

scholarly journals The evaluation of java fine flavor cocoa propagation through somatic embryogenesis technique for germplasm preservation

2021 ◽  
Vol 306 ◽  
pp. 01056
Author(s):  
Sulistyani Pancaningtyas
Keyword(s):  
Somatic Embryogenesis ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Callus Formation ◽  
Basic Structure ◽  
Embryonic Cells ◽  
Regeneration Rate ◽  
Germplasm Preservation ◽  
Embryogenic Callus Formation ◽  
Secondary Somatic Embryos

Somatic embryogenesis is one of the newest technology that applied for the mass production of cocoa. This research aims to evaluate the regeneration rate of somatic embryos through somatic embryogenesis propagation techniques on java fine flavor cocoa. Cultivars in this study are ICCRI 01, ICCRI 02, DR 1, DR 2, DRC 16, DR 38, PNT 16, and PNT 30. Observations include parameters to determine the percentage of primary callus and embryogenic callus formation and the number of somatic embryos produced. Based on data, the ability of callus to produce primary embryos is highly dependent on plant cultivars and explant sources. Five cultivars showed a higher regeneration rate using explants from the petal part, while the rest showed a higher regeneration rate using explants from the staminode section. Embryogenic callus from each cacao cultivar has the same basic structure: a nodular friable structure consisting of many embryonic cells. Some fine flavor cacao cultivars that were able to produce callus and primary somatic embryos could not produce secondary somatic embryos and plantlets. However, two cultivars, which had low potential in producing primary embryos, had the high ability to produce secondary somatic embryos and develop into plantlets.

scholarly journals Partial Organogenesis and Somatic Embryogenesis from Petiole Explants of Field-grown Papaya (Carica papaya L.)

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 629g-630 ◽  
Author(s):  
S. Jayasankar ◽  
U.L Yadava
Keyword(s):  
Somatic Embryogenesis ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Carica Papaya ◽  
Vascular Tissue ◽  
The Other ◽  
Ms Medium ◽  
Free Medium ◽  
Petiole Explants ◽  
Young Leaves

Petiole discs from young leaves of female papaya (L-45) plants were cultured in MS or B5-based media containing 0, 2.25, 4.5, 11.25, and 22.5 μm 2,4-D. Compact embryogenic callus emerged from vascular tissue of petiole discs in about 3 weeks. In MS medium, 66% and 51% explants formed embryogenic callus with 11.25 and 22.5 μm 2,4-D, respectively. On the other hand, 79% explants formed embryogenic callus in B5-based medium with 4.50 μm 2,4-D. However, explants became necrotic in B5-based medium with 22.5 μm 2,4-D. Subculturing callus in auxin-free medium resulted in the development of roots or somatic embryos. Microscopic observations revealed that the roots were produced only by the callus that had retained its continuity with the vascular tissue. This investigation revealed that petioles from field grown papaya plants are potential explants for somatic embryogenesis and 2-week exposure to 2,4-D is adequate for inducing morphogenesis. Additionally, an interaction between 2,4-D and the components in the MS and B5-based media was observed.

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