scholarly journals Diffusion tubes: a method for the mass culture of ctenophores and other pelagic marine invertebrates

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8938 ◽  
Author(s):  
Wyatt L. Patry ◽  
MacKenzie Bubel ◽  
Cypress Hansen ◽  
Thomas Knowles
Keyword(s):  
Mass Culture ◽  
Marine Invertebrates ◽  
Marine Invertebrate ◽  
Water Diffusion ◽  
Food Culture ◽  
Live Food ◽  
Mnemiopsis Leidyi ◽  
Monterey Bay ◽  
Diffusion Tubes ◽  
The 1960S

The culture of pelagic marine invertebrates, especially the ctenophore Mnemiopsis leidyi, has been demonstrated in past studies dating back to the 1960s; however, the mass culture of delicate pelagic invertebrates has remained elusive. By using a pair of acrylic tubes and enabling water diffusion between them, we have been able to reliably and cost effectively mass culture several genera of ctenophores (Pleurobrachia, Hormiphora, Bolinopsis, Mnemiopsis and Leucothea), one species of siphonophore (Nanomia) and one species of larvacean (Oikopleura). The simple, compact method is effective enough to support two permanent exhibits of ctenophores at the Monterey Bay Aquarium while minimizing live food culture requirements with the potential to support further investigation of pelagic marine invertebrate ontogeny, ecology and genomics.

scholarly journals Diffusion tubes: a method for the mass culture of ctenophores and other pelagic marine invertebrates

2019 ◽  
Author(s):  
Wyatt L. Patry ◽  
Mac Kenzie Bubel ◽  
Cypress Hansen ◽  
Thomas Knowles
Keyword(s):  
Mass Culture ◽  
Marine Invertebrates ◽  
Marine Invertebrate ◽  
Water Diffusion ◽  
Food Culture ◽  
Live Food ◽  
Mnemiopsis Leidyi ◽  
Monterey Bay ◽  
Diffusion Tubes

AbstractThe culture of pelagic marine invertebrates, especially the ctenophore Mnemiopsis leidyi, has been demonstrated in past studies dating back to the 1960’s, however the mass culture of delicate pelagic invertebrates has remained elusive. By using a pair of acrylic tubes and enabling water diffusion between them, we have been able to reliably and cost effectively mass culture several genera of ctenophores (Pleurobrachia, Hormiphora, Bolinopsis, Mnemiopsis, and Leucothea), one species of siphonophore (Nanomia) and one species of larvacean (Oikopleura). The simple, compact method is effective enough to support two permanent exhibits of ctenophores at the Monterey Bay Aquarium while minimizing live food culture requirements with the potential to support further investigation of pelagic marine invertebrate ontogeny, ecology and genomics.

scholarly journals Neurotoxicity in Marine Invertebrates: An Update

Biology ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 161
Author(s):  
Irene Deidda ◽  
Roberta Russo ◽  
Rosa Bonaventura ◽  
Caterina Costa ◽  
Francesca Zito ◽  
...  
Keyword(s):  
Nervous System ◽  
Marine Invertebrates ◽  
Marine Pollution ◽  
Marine Invertebrate ◽  
High Sensitivity ◽  
Model Systems ◽  
Neural Genes ◽  
Chemical Agents ◽  
Neurotoxic Effects ◽  
Invertebrate Model

Invertebrates represent about 95% of existing species, and most of them belong to aquatic ecosystems. Marine invertebrates are found at intermediate levels of the food chain and, therefore, they play a central role in the biodiversity of ecosystems. Furthermore, these organisms have a short life cycle, easy laboratory manipulation, and high sensitivity to marine pollution and, therefore, they are considered to be optimal bioindicators for assessing detrimental chemical agents that are related to the marine environment and with potential toxicity to human health, including neurotoxicity. In general, albeit simple, the nervous system of marine invertebrates is composed of neuronal and glial cells, and it exhibits biochemical and functional similarities with the vertebrate nervous system, including humans. In recent decades, new genetic and transcriptomic technologies have made the identification of many neural genes and transcription factors homologous to those in humans possible. Neuroinflammation, oxidative stress, and altered levels of neurotransmitters are some of the aspects of neurotoxic effects that can also occur in marine invertebrate organisms. The purpose of this review is to provide an overview of major marine pollutants, such as heavy metals, pesticides, and micro and nano-plastics, with a focus on their neurotoxic effects in marine invertebrate organisms. This review could be a stimulus to bio-research towards the use of invertebrate model systems other than traditional, ethically questionable, time-consuming, and highly expensive mammalian models.

On the Chemical Form of Mercury in Edible Fish and Marine Invertebrate Tissue

1992 ◽  
Vol 49 (5) ◽  
pp. 1010-1017 ◽  
Author(s):  
Nicolas S. Bloom
Keyword(s):  
Total Mercury ◽  
Marine Invertebrates ◽  
Marine Invertebrate ◽  
Chemical Form ◽  
Weight Basis ◽  
Atomic Fluorescence Spectrometry ◽  
Atomic Fluorescence ◽  
Saltwater Fish ◽  
Relative Standard ◽  
Wet Weight

Total mercury, monomethylmercury (CH3Hg), and dimethylmercury ((CH3)2Hg) in edible muscle were examined in 229 samples, representing seven freshwater and eight saltwater fish species and several species of marine invertebrates using ultraclean techniques. Total mercury was determined by hot HNO3/H2SO4/BrClldigestion, SnCl2 reduction, purging onto gold, and analysis by cold vapor atomic fluorescence spectrometry (CVAFS). Methylmercury was determined by KOH/methanol digestion using aqueous phase ethylation, cryogenic gas chromatography, and CVAFS detection. Total mercury and CH3Hg concentrations varied from 0.011 to 2.78 μg∙g−1 (wet weight basis, as Hg) for all samples, while no sample contained detectable (CH3)2Hg (<0.001 μg∙g−1 as Hg). The observed proportion of total mercury (as CH3Hg) ranged from 69 to 132%, with a relative standard deviation for quintuplicate analysis of about 10%; nearly all of this variability can be explained by the analytical variability of total mercury and CH3Hg. Poorly homogenized samples showed greater variability, primarily because total mercury and CH3Hg were measured on separate aliquots, which vary in mercury concentration, not speciation. I conclude that for all species studied, virtually ail (>95%) of the mercury present is as CH3Hg and that past reports of substantially lower CH3Hg fractions may have been biased by analytical and homogeneity variability.

The ‘civic religion’ of the Resistance in post-war Italy

Modern Italy ◽  
2000 ◽  
Vol 5 (2) ◽  
pp. 113-132 ◽  
Author(s):  
Stephen Gundle
Keyword(s):  
Mass Culture ◽  
Significant Degree ◽  
Artistic Representations ◽  
The First World War ◽  
Civic Religion ◽  
Post War ◽  
Economic Boom ◽  
The 1960S ◽  
High Degree ◽  
First World

SummaryThe problem of the legitimacy or otherwise of the Resistance tradition in post-war Italy has been addressed in recent years mainly in terms of the role of the partisan struggle and its political legacy. This article aims to assess the tradition in terms of commemorative practices, rituals, artistic representations and monuments. It seeks to evaluate whether the Resistance gave rise to a civic religion that may be compared to those which existed in the Liberal period, based on the heroic struggles and figures of the Risorgimento, and the Fascist period, which drew on the feelings of loss and injustice that followed the First World War. It is argued that, although the Resistance lacked, prior to the 1960s, a high degree of official sponsorship, it did acquire some of the features of a civic religion. Its appeal was mainly limited to the regions administered by the Left which had seen a significant degree of Resistance activity in 1943-5. Even here, however, it was difficult to sustain the tradition as a key feature of community life during and after the economic boom: the eclipse of public culture, the decline of public mourning and the development of commercial leisure and mass culture all served to deprive it of meaning. Although intellectuals, politicians and ex-partisans reacted to this situation, the visual and rhetorical languages associated with the commemoration of the Resistance became increasingly divorced from everyday life and dominant social values.

The Folk Fight Back: Protesting the Cancellation of Don Messer’s Jubilee in 1969

2021 ◽  
Author(s):  
Greg Marquis
Keyword(s):  
Popular Culture ◽  
New Brunswick ◽  
Mass Culture ◽  
Generation Gap ◽  
Folk Culture ◽  
Television History ◽  
Canadian Nationalism ◽  
The 1960S

This article examines part of the reaction to the 1969 cancellation of Canadian Broadcasting Corporation’s (cbc) television’s Don Messer’s Jubilee, one of the most popular Canadian-produced programs of the era. In addition to an exploration of television history and popular culture, it is also a look at the neglected topic of “square” Canada in the 1960s. Messer began fiddling at dances in rural New Brunswick in the 1920s and moved to radio and recording prior to becoming an unlikely national television star by 1961. After exploring possible classifications of the show’s music, the article explores themes in protest letters and petitions sent to the cbc. These included Canadian nationalism in opposition to American mass culture, Canadian folk culture, cbc elitism, Maritime regionalism, nostalgia, and the related themes of the generation gap and permissive society. The article concludes that fans viewed Messer as a custodian of Canadian folk culture that was being erased by the national broadcaster at a time of heightened nationalism.

Abrupt mortality of marine invertebrates at the Younger Dryas-Holocene transition in a shallow inlet of the Goldthwait Sea

The Holocene ◽  
2018 ◽  
Vol 28 (12) ◽  
pp. 1894-1908
Author(s):  
Andréanne Bourgeois-Roy ◽  
Hugo Crites ◽  
Pascal Bernatchez ◽  
Denis Lacelle ◽  
André Martel
Keyword(s):  
Mytilus Edulis ◽  
Marine Invertebrates ◽  
Transition Period ◽  
Marine Invertebrate ◽  
Laurentide Ice Sheet ◽  
The North ◽  
Invertebrate Diversity ◽  
Shell Deposit ◽  
The Impact ◽  
Rapid Environmental Change

The late Pleistocene–early Holocene transition period was characterized by rapid environmental change. Here, we investigate the impact of these changes on the marine invertebrates living in a shallow inlet of the post-glacial Goldthwait Sea. The site is located near Baie-Comeau (QC, Canada), where a number of remarkably well-preserved shell deposits are found along the Rivière aux Anglais Valley on the north shore of the St. Lawrence maritime estuary. Seven phyla of marine invertebrates with a minimum of 25 species or taxa were inventoried in a shell deposit, dominated by a community of Hiatella arctica with Mytilus edulis and barnacles composing the subcommunity. The majority of taxa identified in the shell deposit are boreal and sub-Arctic species; however, temperate species that exist today in the St. Lawrence maritime estuary have not been found. Based on marine invertebrate diversity and δ18O(CaCO3) of Mytilus edulis, the water in the shallow inlet of the Goldthwait Sea must have been cold and saline. The range of AMS 14C ages from 15 Mytilus edulis, constrained to 10,900 and 10,690 cal. yr BP, and exceptional state of preservation of adult and juvenile molluscan specimens suggest the abrupt mortality of entire invertebrate communities due to changing hydrodynamic conditions that included the combined effect of freshwater discharge from the receding Laurentide Ice Sheet and rapid isostatic uplift.

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