scholarly journals Effect of Enamel Pre-etching with Sodium Hypochlorite Deproteinisation and Bonding Agent on Retention and Microleakage of Pit and Fissure Sealants: An In-vitro Study

2021 ◽  
Author(s):  
VV Mubeena ◽  
Tharian B Emmatty ◽  
Kumar Kavita ◽  
Bijimole Jose ◽  
AM Riswana
Keyword(s):  
Sodium Hypochlorite ◽  
Artificial Saliva ◽  
Medical Science ◽  
Enamel Surface ◽  
Bonding Agent ◽  
Permanent Teeth ◽  
Acid Etching ◽  
Fissure Sealants ◽  
Pit And Fissure Sealants

Introduction: Sealants have proved to be one of the easiest methods of caries prevention in young permanent teeth, the placement of which is very technique sensitive. The non-invasive method of pretreating the enamel surface by deproteinising it with 5.25% sodium hypochlorite (NaOCl) for 60 seconds prior to etching has proven to be a promising method for improving retention and reducing microleakage of sealant. Aim: To evaluate the effect of enamel pre-etching with sodium hypochlorite deproteinisation and bonding agent on retention and microleakage of pit and fissure sealants. Materials and Methods: This in-vitro experimental study was conducted in Department of Paediatric and Preventive Dentistry at Annoor Dental College, Muvattupuzha, Kerala and Nanotechnology Lab at Amrita Institute of Medical Science. Freshly extracted Intact permanent maxillary and mandibular 20 third molars and 20 premolars mounted on acrylic blocks were divided into 4 groups containing 5 teeth in each group. Molars and premolar were taken for assessing the rate of retention and microleakage, respectively. Teeth in Group 1 were subjected to acid etching only, Group 2 were deproteinised prior to acid etching, Group 3 were subjected to acid etching followed by bonding and Group 4 were subjected to deproteinisation, etching and bonding. Then, all teeth were sealed with pit and fissure sealant and underwent thermocycling in artificial saliva bath for evaluation of sealant retention and microleakage. After thermocycling, retention was assessed on molar by one pre-calibrated blinded examiner by passing a 0.5 mm diameter probe along the margins of the sealant placed to verify integrity, failure, or loss of continuity based on Simonsen’s criteria (1989). To assess microleakage, premolar samples were immersed in rhodamine B, sectioned longitudinally, and examined under a stereomicroscope for assessment of microleakage. Collected data were statistically analysed using Chi-square and Mann-Whitney u test. Results: The results showed 100% retention for teeth in Group 2a, Group 3a, Group 4a, while partial sealant loss was present in 3 teeth from Group 1a. The differences between the groups for retention was statistically significant (p=0.014). In the case of microleakage, Group 3b showed more microleakage and group 4b showed least microleakage and the difference between them was statistically significant (p=0.009). Conclusion: Deproteinisation and use of bonding agent increases retention of the pit and fissure sealants. However, deproteinisation is a more effective method to control microleakage when compared to bonding agent. Thus, it can be considered as an effective method for pretreating enamel surface before sealant placement.

In Vitro Evaluation of Different Protocols for Preventing Microleakage of Fissure Sealants Placed Following Saliva Contamination

2020 ◽  
Vol 44 (4) ◽  
pp. 240-248
Author(s):  
Hayrunnisa Şimşek ◽  
A Rüya Yazıcı ◽  
H Cem Güngör
Keyword(s):  
Artificial Saliva ◽  
Acid Etching ◽  
Fissure Sealants ◽  
Fissure Sealant ◽  
Surface Conditioning ◽  
Etch And Rinse ◽  
Group B ◽  
Saliva Contamination ◽  
Resin Sealant

Purpose: To evaluate the effect of different enamel conditioning protocols and their re-application on the microleakage of fissure sealants placed following saliva contamination. Study design: The study included 156 human third molars in 16 subgroups (2×4×2) under two main groups (sealant type): Group A- hydrophobic resin sealant, 3M Clinpro™ Sealant; Group B- hydrophilic resin sealant, Ultraseal XT Hydro. Each group was then divided according to the type of surface conditioning; 1- Er,Cr:YSSG laser etching, 2- acid-etching, 3- acid-etching+etch-and-rinse adhesive (Prime&Bond® One Select) and 4- self-etching adhesive (Clearfil™ SE Bond). After contaminating the conditioned occlusal enamel surfaces with artificial saliva, fissure sealant was applied in half of the specimens (a), whereas in the other half, (b) the respective surface conditioning was repeated and then fissure sealant was placed. Following thermocycling, the samples were immersed in basic fuchsin, sectioned, and dye penetration was quantitatively assessed with ImageJ. Two-way ANOVA and Bonferroni post-hoc tests were used for statistical analyses (p<0.05). Results: The least microleakage was observed in A3b and A3a, whereas B4b and B4a were the subgroups with the highest microleakage. Following saliva contamination, when surface conditioning was not re-applied, the effects of fissure sealant types and surface conditioning were significant (p=0.005 and p<0.001, respectively). However, their interaction was insignificant (p=0.173). When surface conditioning was re-applied after saliva contamination, the effects of type of fissure sealant and surface conditioning (p=0.000, for both) and their interaction (p=0.004) were significant. Conclusions: 3M Clinpro™ Sealant was superior to Ultraseal XT Hydro. Re-application of Er,Cr:YSSG laser and the self-etching adhesive did not affect the microleakage of both fissure sealants. Without re-application, acid-etching+etch-and-rinse adhesive was superior to acid-etching only. However, both of them were similarly successful when they were re-applied following saliva contamination.

scholarly journals Shear bond strength of pit and fissure sealants on permanent teeth after several etching protocols: In vitro study

2017 ◽  
Vol 54 (3) ◽  
pp. 253-258
Author(s):  
Irina Mézquita-Rodrigo ◽  
Rosalía Contreras-Bulnes ◽  
Laura Rodríguez-Vilchis ◽  
Rogelio Scougall-Vilchis ◽  
Ulises Velazquez-Enriquez ◽  
...  
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
In Vitro Study ◽  
Vitro Study ◽  
Permanent Teeth ◽  
Fissure Sealants ◽  
Pit And Fissure Sealants

SEM Investigation of the Variability of Enamel Surfaces After Simulated Clinical Acid Etching for Pit and Fissure Sealants

1975 ◽  
Vol 54 (6) ◽  
pp. 1222-1231 ◽  
Author(s):  
G.W. Marshall ◽  
L.M. Olson ◽  
C.V. Lee
Keyword(s):  
Surface Relief ◽  
Enamel Surface ◽  
Acid Etching ◽  
Fissure Sealants ◽  
Pit And Fissure Sealants ◽  
Pits And Fissures ◽  
Sem Investigation

Etching preferentially occurs on cuspal inclines and on premolars vs molars. After longer times, etching occurs near the pits and fissures. Enamel rods etch at cores and prism borders simultaneously in the same area. Longer etching times produce more enamel surface relief, permitting better sealant adaption to the enamel.

scholarly journals A Comparative Analysis of Penetration and Adaptation of Two Types of Light Cure Pit and Fissure Sealants: An In-vitro Study

2021 ◽  
pp. 428-432
Author(s):  
Garima Mukesh Sharma ◽  
Jayashree Rajesh Patil ◽  
Priyanka Paul Madhu ◽  
Kumar Gaurav Chhabra ◽  
Amit Reche
Keyword(s):  
Young Patients ◽  
Permanent Teeth ◽  
Tooth Surface ◽  
Fissure Sealants ◽  
Occlusal Caries ◽  
Fissure Sealant ◽  
Pit And Fissure Sealants ◽  
Group I ◽  
Pit And Fissure Sealant

Background: Pit and fissure sealants are well-known for their ability to prevent occlusal caries. Given the difficulties of maintaining complete isolation for longer periods of time when treating young patients, a streamlined sealant application process is needed. As a result, the goal of the present study is to compare the penetration and adaption of 2 types of light cure sealants. Objectives: To evaluate the adaptation and penetration of Conseal f ‘pit and fissure sealant’. To evaluate the adaptation and penetration of PF seal SE ‘pit and fissure sealant’. To evaluate comparison of adaptation and penetration between two types of light cure sealants. Methodology: This will be an in-vitro comparative study, which will consist of 2 categories - Group I (acid-etch fissure sealant, Conseal f) and Group II (self-etch fissure sealant, PF seal SE). A total of 20 extracted permanent teeth will then be classified into 2 groups of 10 teeth respectively and stereomicroscope will be used to compare the penetration and adaptation between two types of light cure pit and fissure sealants. Expected Results: The study will evaluate the penetration and adaptation of two types of light cure sealants on the tooth surface which will provide the efficacy of the sealant. Conclusion: The study will determine the sealing and penetration and adaption capacity of the sealant by the light cure method. And the sealants are beneficial and biocompatible in preventing, stopping the advancement of non-cavitated carious lesions. Additional study is needed to determine the relative advantages of the various categories of sealant material. In practice, the benefits of sealing should be examined locally, and practitioners should follow specific standards.

scholarly journals Microleakage of a Self-Adhesive Flowable Composite, a Self-Adhesive Fissure Sealant and a Conventional Fissure Sealant in Permanent Teeth with/without Saliva Contamination

2019 ◽  
Author(s):  
Zohre Sadat Hosseinipour ◽  
Alireza Heidari ◽  
Mehdi Shahrabi ◽  
Kiana Poorzandpoush
Keyword(s):  
Practical Method ◽  
Permanent Teeth ◽  
Third Molars ◽  
Fissure Sealants ◽  
Fissure Sealant ◽  
Dye Penetration ◽  
Flowable Composite ◽  
Saliva Contamination ◽  
Vertise Flow

Objectives: Application of fissure sealants is a practical method for prevention of occlusal pit and fissure caries. Microleakage is an important factor affecting the success of fissure sealant treatment. This study aimed to assess the microleakage of a self-adhesive flowable composite, a self-adhesive fissure sealant and a conventional fissure sealant in permanent teeth with/without saliva contamination. Materials and Methods: This in vitro, experimental study evaluated 108 extracted human third molars, which were randomly divided into six groups (n=18) of control, Denu-Seal conventional fissure sealant applied on etched enamel with/without saliva contamination, Vertise Flow self-adhesive flowable composite applied on rinsed and dried enamel with/without saliva contamination and Prevent Seal self-adhesive fissure sealant applied on rinsed and dried enamel with/without saliva contamination. Microleakage was assessed using the dye penetration technique and subsequent observation of samples under a stereomicroscope. Data were analyzed using the Kruskal-Wallis and Tukey’s test. Results: Microleakage of the conventional fissure sealant with/without saliva contamination was significantly lower than that of other groups (P<0.05). Conclusion: Microleakage of the conventional fissure sealant is less than that of self-adhesive fissure sealant and self-adhesive composite, irrespective of saliva contamination.

scholarly journals Comparative Evaluation of Fluoride Release From Chemically Cured and Light-Cured Orthodontic Bonding Agents and Surface Alteration of Enamel: An in vitro Study

2020 ◽  
Vol 54 (3) ◽  
pp. 233-239
Author(s):  
Ashish Kumar Barik ◽  
Ritu Duggal
Keyword(s):  
Artificial Saliva ◽  
In Vitro Study ◽  
Fluoride Content ◽  
Bonding Agent ◽  
Fluoride Release ◽  
Bonding Agents ◽  
Surface Alteration ◽  
Orthodontic Bonding ◽  
Group B

Introduction: Fluoride is known to reduce demineralization and enhance remineralization which is desirable around orthodontic brackets. Material and methods: This study was carried out to determine the rate of fluoride release, fluoride content of enamel, and surface alteration of enamel from orthodontic bonding agents. Thirty extracted maxillary first premolars were divided into 2 groups of 15 teeth each. Group A—teeth were bonded with a chemically cured orthodontic bonding agent containing fluoride (Rely-a-Bond, Reliance, Itasca) and group B—teeth were bonded with a light-cured orthodontic bonding agent containing fluoride (Light Bond Reliance, Itasca). All individual sample teeth were used to estimate fluoride release and fluoride content of enamel. The same samples were also used to evaluate enamel surface alterations. The rate of fluoride release from the above 2 orthodontic bonding agents were measured in artificial saliva on day 1, 2, 3, 4, 5, 6, 7, 14, 30, 60, and 90. The fluoride content of the enamel was evaluated on day “0” and day “90” of bonding. The samples were also evaluated for the surface changes in enamel using scanning electron microscope (SEM). Results: The results showed that (a) chemically cured and light-cured bonding agent showed a dramatic decrease in the concentration of fluoride release from day “1” to day “2,” (b) the concentration of fluoride release kept decreasing from day “1” to day “90” of measurement in both the groups, and (c) SEM of the bracket margins bonded with chemically cured bonding agent and light-cured bonding agent revealed particle depositions of irregular shape and size on day “90.” Conclusion: Light-cured bonding agent (Light Bond) released higher concentration of fluoride as compared to chemically cured bonding agent (Rely-a-Bond). Light-cured bonding agent (Light Bond) showed higher increase in fluoride content of enamel than chemically cured bonding agent (Rely-a-Bond).

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