scholarly journals A Comparative Analysis of Penetration and Adaptation of Two Types of Light Cure Pit and Fissure Sealants: An In-vitro Study

2021 ◽  
pp. 428-432
Author(s):  
Garima Mukesh Sharma ◽  
Jayashree Rajesh Patil ◽  
Priyanka Paul Madhu ◽  
Kumar Gaurav Chhabra ◽  
Amit Reche
Keyword(s):  
Young Patients ◽  
Permanent Teeth ◽  
Tooth Surface ◽  
Fissure Sealants ◽  
Occlusal Caries ◽  
Fissure Sealant ◽  
Pit And Fissure Sealants ◽  
Group I ◽  
Pit And Fissure Sealant

Background: Pit and fissure sealants are well-known for their ability to prevent occlusal caries. Given the difficulties of maintaining complete isolation for longer periods of time when treating young patients, a streamlined sealant application process is needed. As a result, the goal of the present study is to compare the penetration and adaption of 2 types of light cure sealants. Objectives: To evaluate the adaptation and penetration of Conseal f ‘pit and fissure sealant’. To evaluate the adaptation and penetration of PF seal SE ‘pit and fissure sealant’. To evaluate comparison of adaptation and penetration between two types of light cure sealants. Methodology: This will be an in-vitro comparative study, which will consist of 2 categories - Group I (acid-etch fissure sealant, Conseal f) and Group II (self-etch fissure sealant, PF seal SE). A total of 20 extracted permanent teeth will then be classified into 2 groups of 10 teeth respectively and stereomicroscope will be used to compare the penetration and adaptation between two types of light cure pit and fissure sealants. Expected Results: The study will evaluate the penetration and adaptation of two types of light cure sealants on the tooth surface which will provide the efficacy of the sealant. Conclusion: The study will determine the sealing and penetration and adaption capacity of the sealant by the light cure method. And the sealants are beneficial and biocompatible in preventing, stopping the advancement of non-cavitated carious lesions. Additional study is needed to determine the relative advantages of the various categories of sealant material. In practice, the benefits of sealing should be examined locally, and practitioners should follow specific standards.

scholarly journals Clinical evaluation of hydrophobic and hydrophilic pit and fissure sealants among 7-10 year old school children: a split mouth study design

2021 ◽  
Vol 24 (2) ◽  
Author(s):  
Indra Priyadharshini ◽  
Kiran Iyer ◽  
Madankumar Parangimalai Diwakar
Keyword(s):  
Surface Roughness ◽  
School Children ◽  
Marginal Adaptation ◽  
Fissure Sealants ◽  
Fissure Sealant ◽  
Pit And Fissure Sealants ◽  
Group I ◽  
Occlusal Surfaces ◽  
Colour Match ◽  
Pit And Fissure Sealant

Introduction: The study was done to compare the effectiveness of hydrophobic and hydrophilic pit and fissure sealant retention among 7-10 year old school children. Material and Methods: The present split mouth randomized trial was conducted on the occlusal surfaces of permanent first mandibular molars to compare and assess the retention along with other outcome variables like colour match, marginal discolouration, marginal adaptation, anatomic form, postoperative sensitivity, caries formation and surface roughness properties of Hydrophobic and Hydrophilic pit and fissure sealants at 3rd and 6th month. The data was compiled and analyzed using SPSS software and results were generated. Results: In the present study among the 50 restorations in the hydrophobic pit and fissure sealants (Group I), 48 (96%) restorations were retained at the end of third month and 45(90%) were retained at the end of sixth month. Among the 50 restorations in the hydrophilic pit and fissure sealants (Group II), 49(98%) restorations were retained at the end of third month, and 46(92%) at the end of sixth month were retained. There was no significant difference in the number of completely retained restorations at the end of six months (p = 1.00) among both the groups. The assessment of all the other outcome variables between both the interventions showed that the difference was not statistically significant. Conclusion: The study concluded that the hydrophilic pit and fissure sealant was similar to the hydrophobic pit and fissure sealant in terms of retention, colour match, marginal discolouration, marginal adaptation, anatomic form and surface roughness properties with.   Keywords Pit and fissure sealants; Moisture tolerant; 7-10 years; School children; Mandibular teeth.

scholarly journals Resin-based sealants longevity: a clinical evaluation

2020 ◽  
Vol 9 (3) ◽  
Author(s):  
Heitor Ceolin Araujo ◽  
Isabela Copetti Faria ◽  
Brenda Zanfolin Torquato ◽  
Renan Ceolin Araujo ◽  
Rosana Leal do Prado ◽  
...  
Keyword(s):  
Dental Caries ◽  
Clinical Evaluation ◽  
Permanent Teeth ◽  
Caries Experience ◽  
Fissure Sealants ◽  
Fissure Sealant ◽  
Pit And Fissure Sealants ◽  
Significant Difference ◽  
Permanent Molars ◽  
Pit And Fissure Sealant

Introdution: The occlusal surface of the molars are more susceptible to dental caries because of their anatomy, so in some cases, the sealants are indicated. Objective: The objective of this study was to evaluate the efficacy, retention, presence of caries and marginal discoloration of resinous sealants (Fluoroshield and Prevent) in first permanent molars. Methods: Ninety - one children aged 7 to 9 years were selected from a municipal institution Regente Feijó, SP, Brazil, for the application of resinous sealants to first permanent lower first molars. The application of Fluoroshield and Prevent resin sealants was performed according to the manufacturer's recommendations. The evaluation was performed after 6 and 12 months by double-blind examiners and followed the criteria: alpha (total sealant, absence of caries and absence of pigmentation) charlie (partial sealing, superficial caries and light pigmentation) charlie complete sealing, presence of cavitation and pigmentation) after 12 months of application by two previously calibrated examiners. Results: Using Fischer's exact test, no significant difference (p 0.05) was observed between the resin-based sealant groups. In the evaluated criteria, both sealants after three years were similar, and may therefore be suitable materials for sealing grooves and fissures in permanent molars.Descriptors: Pit and Fissure Sealants; Dental Caries; Dentition, Permanent; Oral Health.ReferencesPalma-Dibb RG, Chinelatti MA, Souza-Zaroni WC. Diagnóstico de lesões de cárie. In: Assed S. Odontopediatria: bases científicas para a prática clínica. São Paulo: Artes Médicas; 2005.Sheiham A, James WP. Diet and Dental Caries: The Pivotal Role of Free Sugars Reemphasized. J Dent Res. 2015;94(10):1341-47.Faleiros Chioca S, Urzúa Araya L, Rodríguez Martínez G, Cabello Ibacache R. Uso de sellantes de fosas y fisuras para La prevención de caries em población infanto-juvenil: Revisión metodológica de ensayos clínicos. Rev Clin Periodoncia Implantol Rehabil Oral. 2013;6(1):14-9.Liu BY, Lo EC, Chu CH, Lin HC. Randomized Trial on Fluorides and Sealants for Fissure Caries Prevention. J Dent Res 2012;91(8):753-58Condò R, Cioffi A, Riccio A, Totino M, Condò SG, Cerroni L. Sealants in dentistry: a systematic review of the literature. Oral Implantol (Rome). 2014;6(3):67-74. Ahovuo-Saloranta A, Forss H, Walsh T, Hiiri A, Nordblad A, Mäkelä M et al.  Sealants for preventing dental decay in the permanent teeth. Cochrane Database Syst Rev. 2013;(3):CD001830Moreira KMS,Kantovitz KR,Aguiar JPD, Borges AFS, Pascon FM, Puppin-Rontani RM.  Impact of the intermediary layer on sealant retention: a randomized 24-month clinical trial. Clin Oral Investig. 2017;21(5):1435-43.Griffin SO, Gray SK, Malvitz DM, Gooch BF. Caries Risk in Formerly Sealed Teeth. J Am Dent Assoc 2009;140(4):415-23.Splieth CH, Ekstrand KR, Alkilzy M, Clarkson J, Meyer-Lueckel H, Martignon S et al. Sealants in dentistry: outcomes of the ORCA Saturday Afternoon Symposium 2007. Caries Res. 2010;44(1):3-13Sundfeld RH, Briso ALF, Mauro SJ, de Alexandre RS, Sundfeld Neto D, Oliveira FG et al. Twenty years experience with pit and fissure sealants. Int J Clin Dent. 2010;2(4):1-12.Baldini V, Tagliaferro EPS, Ambrosano GMB, Meneghim MC, Pereira AC. Use of occlusal sealant in a community program and caries incidence in high- and low-risk children. J Appl Oral Sci. 2010;19(4):396-402.Provenzano MGA, Rios D, Fracasso MLC, Marchesi A, Honório HM. Clinical Evaluation of a Resin-Modifi ed Glass Ionomer Cement (Vitremer®) Used as Pit-And-Fissure Sealant in Primary Molars. Pesq Bras Odontoped Clin Integr.2010;10(2):233-40.Saito CM, Lima EP, Mello D, Mello FAS. Selante resinoso: tratamento preventivo e  minimamente invasivo. Rev Gest Saúde. 2014;11:10-17.Zenkner JE, Alves LS, de Oliveira RS, Bica RH, Wagner MB, Maltz M. Influence of eruption stage and biofilm accumulation on occlusal caries in permanent molars: a generalized estimating equations logistic approach. Caries Res. 2013;47(3):177-82.Delmondes FS, Imparato JCP. Selamento de primeiros molares permanentes em erupção com cimento de ionômero de vidro. J Bras Odontopediatr. Odontol Bebê. 2003;6(33):373-78.Heyduck C, Meller C, Schwahn C, Spliet CH. Effectiveness of Sealants in Adolescents with High and Low Caries Experience. Caries Res. 2006;40(5):375-81.Tagliaferro EPS, Ambrosano GMB, Meneghim MC, Pereira AC. Risk indicators and risk predictors of dental caries in schoolchildren. J Appl Oral Sci. 2008;16(6):408-13.Silva RCSP, Araujo MAM, Rego MA. Avaliação clinica de selantes de fossulas e fissuras: efeitos de materiais e tempo de analise. Rev Odontol UNESP. 1996;25(2):237-45.Beraldo DZ, Pereira KFS, Zafalon EJ, Yoshinari FMS.Análise comparativa entre selante resinoso e selante ionomérico por microscópio eletrônico de varredura. Rev Odontol UNESP. 2015;44(4):239-43.Araújo IT, Cunha MMF, Vasconcelos MG, Vasconcelos, RG. Selantes: uma técnica eficaz na prevenção da cárie. Com ciênc saúde. 2013;24(3):259-66.Moura SK, Lemos LVFM, Myszkovisk S, Provenzano MGA, Balducci I, Myaki SI. Bonding durability of dental sealants to deciduous and permanent teeth. Braz J Oral Sci. 2014; 13(3):198-202.Sundfeld RH, Mauro SJ, Briso ALF, Sundfeld  MLMM. Clinical/photographic evaluation of a single application of two sealants after eleven years. Bull Tokyo Dent Coll. 2004;45(2):67-75.Folke BD, Walton JL, Feigal RJ. Occlusal Sealant Success Over Ten Years in a Private Practice: Comparing Longevity of Sealants Placed by Dentists, Hygienists, and Assistants. Pediatr Dent. 2004;26(5):426-32.Sundfeld RH, Croll Theodore P, José MS, Briso ALF, Sversut AR, Sundefeld MLMM. Longitudinal photographic observation of the occurrence of bubbles in pit and fissure sealants. J Appl Oral Sci. 2006;14(1):27-32.Arhakis A, Damianaki S, Toumba KJ. Pit and fissure sealants: types, effectiveness, retention, and fluoride release: a literature review. Balkan J Stomatol. 2007;11(3):151-62.Garbin CAS, Garbin AJI, Santos KT, Pizzato E, Moroso TT. Retention of a pit-and-fissure sealant: comparison of three types of isolation. Pesq Bras Odontoped Clin Integr. 2008;8(2):175-78.Kühnisch J, Mansmannb U, Roswitha HW, Hickel R. Longevity of materials for   pit and fissure sealing - results from a meta-analysis. Dent Mater. 2012;28(3):298-303.

scholarly journals An In vitro Comparative Evaluation of Microleakage of ACP Containing Pit and Fissure Sealant and Moisture Tolerant Pit and Fissure Sealant

2021 ◽  
pp. 274-280
Author(s):  
Rachna Mulani ◽  
Aditi Mathur
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
In Vivo Studies ◽  
Blue Dye ◽  
Fissure Sealants ◽  
Fissure Sealant ◽  
Pit And Fissure Sealants ◽  
Occlusal Surfaces ◽  
Pit And Fissure Sealant

Background: Pits and fissure sealants are widely used to prevent caries in children. Microleakage is one of the most crucial factors resulting in sealant compromise. Factors pertaining to microleakage like, pre-treatment of occlusal surfaces, moisture control, bonding systems, and flowability of the sealant, will determine the longevity of the treatment. In vitro microleakage studies are useful in predicting the marginal sealing ability of pit and fissure sealants. Aim: This in vitro study was undertaken to evaluate and compare the microleakage of ACP (Aegis®) containing pit and fissure sealant and moisture tolerant pit and fissure sealant (EmbraceTM WetbondTM). Study Design: In vitro study. Place and Duration of Study: Department of Pedodontics and Preventive Dentistry, Dr. D.Y. Patil Dental College and Hospital, Pune. between June 2020 and February 2021. Methodology: 26 permanent non- carious premolars extracted for orthodontic treatment were used. The sealants used for the analysis of microleakage were Aegis® and EmbraceTM WetbondTM pit and fissure sealants. The premolars were divided into two groups of 13 each. After sealant application, they were immersed in methylene blue dye. The teeth were subjected to thermocycling for 24 hours, after which The teeth were sectioned buccolingually and dye penetration was studied under a stereomicroscope (magnification 10X). Results: Both the groups were studied and statistically evaluated using Mann- Whitney U test. Aegis® showed higher microleakage in (46.2%) than Embrace (38%), though the results were not statistically significant. Conclusion: Both the materials showed microleakage, more In-vivo studies with longer follow- up periods are needed to evaluate and compare the clinical success of these pit & fissure sealants.

scholarly journals Pit and fissure sealants penetration capacity and their correlation with fissure morphology

2019 ◽  
Vol 92 ◽  
pp. S50-S54
Author(s):  
Alexandrina Muntean ◽  
Meda-Romana Simu ◽  
Raluca Suhani ◽  
Anca Stefania Mesaros
Keyword(s):  
In Vitro Study ◽  
Glass Ionomer ◽  
Fissure Sealants ◽  
Depth Of Penetration ◽  
Fissure Sealant ◽  
Pit And Fissure Sealants ◽  
Group I ◽  
Occlusal Surfaces ◽  
Resin Modified Glass Ionomer

Background and aims. Pit and fissure sealant placement corresponds to an effective approach of the prevention of caries on occlusal surfaces. Fissure morphology and dental material characteristics are the key factors for sealant effectiveness. The aim of this in vitro study was to compare the penetration ability of two commercially available pit and fissure sealants. Methods. Twenty sound human premolars extracted for orthodontic purpose have been sealed according to manufacturer’s instructions as follows: Group I (n=10), light-cured unfilled resin-based sealant, Admira Seal® (Voco GmbH); Group II (n=10), resin modified glass ionomer sealant without varnish, GC Fuji Triage® (GC Corporation). The teeth have been sectioned buccal-lingually in the middle of the occlusal surface, and the sections were examined at 40x with an inverted microscope. Results. Penetration of the sealants was found to be greater in U-type fissure pattern (91.69%) followed by V-type (75.42%), IK-type (71.24%) and then in I-type (63.98%). The depth of penetration of GC Fuji Triage® (82.85%) demonstrated to be superior to Admira Seal® (76.28%). Conclusion. U fissure design was more common than other fissure patterns and showed significantly higher penetration for the two type of sealants evaluated. Resin modified glass ionomer sealant used in the present study perform comparably with the filled resin sealant.

scholarly journals Microleakage of a Self-Adhesive Flowable Composite, a Self-Adhesive Fissure Sealant and a Conventional Fissure Sealant in Permanent Teeth with/without Saliva Contamination

2019 ◽  
Author(s):  
Zohre Sadat Hosseinipour ◽  
Alireza Heidari ◽  
Mehdi Shahrabi ◽  
Kiana Poorzandpoush
Keyword(s):  
Practical Method ◽  
Permanent Teeth ◽  
Third Molars ◽  
Fissure Sealants ◽  
Fissure Sealant ◽  
Dye Penetration ◽  
Flowable Composite ◽  
Saliva Contamination ◽  
Vertise Flow

Objectives: Application of fissure sealants is a practical method for prevention of occlusal pit and fissure caries. Microleakage is an important factor affecting the success of fissure sealant treatment. This study aimed to assess the microleakage of a self-adhesive flowable composite, a self-adhesive fissure sealant and a conventional fissure sealant in permanent teeth with/without saliva contamination. Materials and Methods: This in vitro, experimental study evaluated 108 extracted human third molars, which were randomly divided into six groups (n=18) of control, Denu-Seal conventional fissure sealant applied on etched enamel with/without saliva contamination, Vertise Flow self-adhesive flowable composite applied on rinsed and dried enamel with/without saliva contamination and Prevent Seal self-adhesive fissure sealant applied on rinsed and dried enamel with/without saliva contamination. Microleakage was assessed using the dye penetration technique and subsequent observation of samples under a stereomicroscope. Data were analyzed using the Kruskal-Wallis and Tukey’s test. Results: Microleakage of the conventional fissure sealant with/without saliva contamination was significantly lower than that of other groups (P<0.05). Conclusion: Microleakage of the conventional fissure sealant is less than that of self-adhesive fissure sealant and self-adhesive composite, irrespective of saliva contamination.

scholarly journals Effect of Enamel Pre-etching with Sodium Hypochlorite Deproteinisation and Bonding Agent on Retention and Microleakage of Pit and Fissure Sealants: An In-vitro Study

2021 ◽  
Author(s):  
VV Mubeena ◽  
Tharian B Emmatty ◽  
Kumar Kavita ◽  
Bijimole Jose ◽  
AM Riswana
Keyword(s):  
Sodium Hypochlorite ◽  
Artificial Saliva ◽  
Medical Science ◽  
Enamel Surface ◽  
Bonding Agent ◽  
Permanent Teeth ◽  
Acid Etching ◽  
Fissure Sealants ◽  
Pit And Fissure Sealants

Introduction: Sealants have proved to be one of the easiest methods of caries prevention in young permanent teeth, the placement of which is very technique sensitive. The non-invasive method of pretreating the enamel surface by deproteinising it with 5.25% sodium hypochlorite (NaOCl) for 60 seconds prior to etching has proven to be a promising method for improving retention and reducing microleakage of sealant. Aim: To evaluate the effect of enamel pre-etching with sodium hypochlorite deproteinisation and bonding agent on retention and microleakage of pit and fissure sealants. Materials and Methods: This in-vitro experimental study was conducted in Department of Paediatric and Preventive Dentistry at Annoor Dental College, Muvattupuzha, Kerala and Nanotechnology Lab at Amrita Institute of Medical Science. Freshly extracted Intact permanent maxillary and mandibular 20 third molars and 20 premolars mounted on acrylic blocks were divided into 4 groups containing 5 teeth in each group. Molars and premolar were taken for assessing the rate of retention and microleakage, respectively. Teeth in Group 1 were subjected to acid etching only, Group 2 were deproteinised prior to acid etching, Group 3 were subjected to acid etching followed by bonding and Group 4 were subjected to deproteinisation, etching and bonding. Then, all teeth were sealed with pit and fissure sealant and underwent thermocycling in artificial saliva bath for evaluation of sealant retention and microleakage. After thermocycling, retention was assessed on molar by one pre-calibrated blinded examiner by passing a 0.5 mm diameter probe along the margins of the sealant placed to verify integrity, failure, or loss of continuity based on Simonsen’s criteria (1989). To assess microleakage, premolar samples were immersed in rhodamine B, sectioned longitudinally, and examined under a stereomicroscope for assessment of microleakage. Collected data were statistically analysed using Chi-square and Mann-Whitney u test. Results: The results showed 100% retention for teeth in Group 2a, Group 3a, Group 4a, while partial sealant loss was present in 3 teeth from Group 1a. The differences between the groups for retention was statistically significant (p=0.014). In the case of microleakage, Group 3b showed more microleakage and group 4b showed least microleakage and the difference between them was statistically significant (p=0.009). Conclusion: Deproteinisation and use of bonding agent increases retention of the pit and fissure sealants. However, deproteinisation is a more effective method to control microleakage when compared to bonding agent. Thus, it can be considered as an effective method for pretreating enamel surface before sealant placement.

scholarly journals Effect of Saliva/Blood Contamination on Enamel Bond Strength

2021 ◽  
Vol 23 (4) ◽  
pp. 277-281
Author(s):  
Rodrigo Galo ◽  
Isabela Maluta ◽  
Marta Maria Martins Giamatei Contente ◽  
Carolina Paes Torres ◽  
Maria Cristina Borsatto
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Testing Machine ◽  
Adhesive System ◽  
Single Bond ◽  
Fissure Sealants ◽  
Pit And Fissure Sealants ◽  
Group I ◽  
Group Ii

AbstractIn the routine clinical situation, the contamination by blood and/or saliva in restorative procedures can be happen in non-cooperation of the patient in dental office. The aim of the study was to assess in vitro shear bond strength of a resin sealant associated with two types of adhesives contaminated with saliva and blood. Healthy human molars were used and the specimens and the crowns were sectioned in the bucco-lingual direction, thus obtaining two segments of similar proportions (mesial and distal), totaling 60 surfaces, and the surfaces were randomly divided into 4 groups (n = 15). Group I (control) received no type of contamination and the sealant was applied. In group II, the surfaces were contaminated with 10 μl of saliva/blood and the sealant was applied. In group III, the surfaces were contaminated with 10 μl of saliva/blood and the Single Bond total-etch adhesive system was applied followed by application of sealant. In group IV, the surfaces were contaminated with 10 μl of saliva/blood and the Prime & Bond NT total-etch adhesive system was applied followed by the application of sealant. Samples were tested in the universal testing machine and the analysis of shear bond strength was performed. A difference between Group I (12.61MPa) and the other groups was found; Group II (2. 28MPa) was different than Groups III (7.07MPa) and IV (7.79MPa), but Groups III and IV were similar. The application of an adhesive system when there is contamination with saliva/blood is required prior to application of pit and fissure sealants. Keywords: Pit and Fissure Sealants. Biological Contamination. Shear Strength. ResumoNa situação clínica de rotina, a contaminação por sangue e/ou saliva em procedimentos restauradores pode ocorrer em pacientes que não colaboram no consultório odontológico. O objetivo do estudo foi avaliar a resistência ao cisalhamento in vitro de um selante de resina associado a dois tipos de adesivos contaminados com saliva e sangue. Foram utilizados molares humanos saudáveis e os espécimes e as coroas foram seccionados na direção bucal-lingual, obtendo assim dois segmentos de proporções semelhantes (mesial e distal), totalizando 60 superfícies, e as superfícies foram divididas aleatoriamente em 4 grupos (n = 15). O Grupo I (controle) não recebeu nenhum tipo de contaminação e o selante foi aplicado. No grupo II, as superfícies foram contaminadas com 10 μl de saliva / sangue e o selante foi aplicado. No grupo III, as superfícies foram contaminadas com 10 μl de saliva / sangue e o sistema adesivo Single-Bond foi aplicado seguindo a aplicação de selante. No grupo IV, as superfícies foram contaminadas com 10 μl de saliva / sangue e o sistema adesivo de ataque total Prime & Bond NT foi aplicado seguido da aplicação de vedante. As amostras foram testadas na máquina de ensaio universal e a análise da resistência à ligação ao cisalhamento foi realizada. Uma diferença entre o Grupo I (12,61MPa) e os outros grupos foi encontrada; O Grupo II (2,28 MPa) foi diferente dos Grupos III (7,07MPa) e IV (7,79 MPa), mas os Grupos III e IV foram semelhantes. A aplicação de um sistema adesivo quando existe contaminação com saliva / sangue é necessária antes da aplicação de selantes de fissura e fissura.. Palavras-chave: Selantes de Fossas e Fissuras. Contaminação Biológica. Resistência ao Cisalhamento.

scholarly journals Influence of Isolation Methods on Retention of Pit and Fissure Sealants in Young Permanent Teeth based on Simonsen’s Criteria: A Randomised Clinical Trial

2021 ◽  
Author(s):  
Madhuri Bandi ◽  
Sreekanth Kumar Mallineni ◽  
Sivakumar Nuvvula
Keyword(s):  
Permanent Teeth ◽  
Treatment Modalities ◽  
Fissure Sealants ◽  
Pit And Fissure Sealants ◽  
Rubber Dam ◽  
Marginal Integrity ◽  
Group I ◽  
Isolation Methods ◽  
Group Ii ◽  
Cotton Roll

Introduction: Pit and Fissure Sealants (PFS) are chemically-active liquid materials that are introduced into the occlusal surface of caries-susceptible teeth. These form a micro mechanically bonded, protective layer preventing access to caries-producing bacteria, from rest of the oral environment. Aim: To evaluate the retention of PFS placed on young permanent teeth using two isolation methods. Materials and Methods: This clinical study was carried out in the Department of Paediatric and Preventive Dentistry, at Narayana Dental College and Hospital, Nellore, Andhra Pradesh, India. One hundred Permanent First Molars (PFM) from 25 healthy, cooperative children in the age group of 6-9 years were assigned by block randomisation into two groups (isolation type); each group containing the upper and lower PFMs of the right and left side receiving two different treatment modalities (split-mouth design). In Group I (n=50 teeth), the teeth received sealant with rubber dam isolation, and in Group II (n=50 teeth), with cotton roll isolation. The sealant was applied to all the PFMs as indicated. All the teeth were evaluated using Simonsen’s criteria at regular intervals of 3, 6, and 12 months. The data collected were subjected to statistical analysis using the SPSS statistical package (version 17) and statistical significance was set at 0.05. The difference in the retention of PFS with the two isolation methods was analysed using the Chi-square test. Results: After 12 months, the marginal integrity rate for sealants placed with a rubber dam and cotton roll isolation were 75% and 64%, respectively. The results were better in Group I than in Group II, although there were no statistically significant differences with respect to marginal integrity, discolouration, and anatomical failure. Conclusion: Retention of PFS in young permanent teeth was better with rubber dam than cotton rolls for isolation, though the results were not statistically significant.

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