scholarly journals Differences in Whole Salivary Total Protein Concentration and Protein Fractions among the Groups of Dentulous Subjects, Edentulous Subjects and Periodontitis Patients

2004 ◽  
Vol 48 (5) ◽  
pp. 723-732 ◽  
Author(s):  
Yukiko Takahashi ◽  
Akihiko Shiba ◽  
Kiyoko Shiba
Keyword(s):  
Total Protein ◽  
Protein Concentration ◽  
Protein Fractions ◽  
Total Protein Concentration

Evaluation of serum total protein concentration and protein fractions in sheep naturally infected with Babesia ovis

2012 ◽  
Vol 23 (1) ◽  
pp. 151-155 ◽  
Author(s):  
B. Esmaeilnejad ◽  
M. Tavassoli ◽  
S. Asri-Rezaei ◽  
B. Dalir-Naghadeh ◽  
S. H. Pourseyed
Keyword(s):  
Total Protein ◽  
Protein Concentration ◽  
Protein Fractions ◽  
Total Protein Concentration ◽  
Serum Total Protein ◽  
Babesia Ovis

scholarly journals Total protein, selected protein fractions and chemical elements in the colostrum and milk of mares (Short Communication)

2009 ◽  
Vol 52 (1) ◽  
pp. 1-6
Author(s):  
A. Cieśla ◽  
R. Palacz ◽  
J. Janiszewska ◽  
D. Skórka
Keyword(s):  
Environmental Conditions ◽  
Total Protein ◽  
Protein Fraction ◽  
Protein Concentration ◽  
Short Communication ◽  
Chemical Elements ◽  
Protein Fractions ◽  
Total Protein Concentration ◽  
Dynamic Changes ◽  
Milk Samples

Abstract. The study material consisted of the colostrum and milk of five noble half-blood mares housed under the same environmental conditions. Colostrum samples were collected 24 h after foaling, whereas milk samples were taken five times, every 30 days, with the first sampling on lactation day 30 (1st month). In the samples were determined the concentrations of total protein, pre-albumins, albumins, α- and β-globulins, immunoglobulins and calcium (Ca), magnesium (Mg) and zinc (Zn). The presence of pre-albumins was observed only in mare colostrum. Total protein concentration was at the same level in the colostrum and in the milk in the 1st lactation month, whereas protein fraction concentrations underwent dynamic changes during the 1st month of lactation. When compared to the values determined in the colostrum, the level of α-globulins increased 8 times during lactation, whereas that of immunoglobulins decreased almost 4 times. The highest concentrations of Ca, Mg and Zn were found in mare colostrum, with a decrease in the concentration of all chemical elements in the 1st month of lactation, confirmed statistically in case of Mg and Zn.

Determination of Total Protein Concentration in Solution Using Gold Electrode Modified with Silver Nanoparticles

2014 ◽  
Vol 27 (1) ◽  
pp. 253-257 ◽  
Author(s):  
Patrick Marcel Seumo Tchekwagep ◽  
Charles Péguy Nanseu-Njiki ◽  
Emmanuel Ngameni ◽  
Ravi Danielsson ◽  
Thomas Arnebrant ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Total Protein ◽  
Gold Electrode ◽  
Protein Concentration ◽  
Total Protein Concentration

Albumin fraction and measurement of total protein concentration

1993 ◽  
Vol 264 (5) ◽  
pp. H1723-H1726 ◽  
Author(s):  
B. T. Peterson ◽  
R. W. Tate
Keyword(s):  
Total Protein ◽  
Protein Concentration ◽  
Gamma Globulin ◽  
Colorimetric Assay ◽  
Full Range ◽  
Computational Method ◽  
Total Protein Concentration ◽  
Albumin Fraction ◽  
Standard Curve ◽  
Protein Assays

The standard curve of a typical colorimetric assay for total protein is often nonlinear and dependent on the albumin fraction of the protein standard. We developed a simple mathematical transformation to make the standard curve linear and a computational method to correct for differences in albumin concentrations among the samples. This method uses data from total protein assays on two sets of standards (albumin and gamma globulin) and provides accurate measures of total protein over the full range of albumin fractions. Comparison of this two-standard method with the a method that uses only albumin as a standard shows that this method prevents physiologically significant overestimations in total protein concentration and calculated protein osmotic pressure differences in the lungs.

scholarly journals Role of fibronectin under conditions of doxorubicin action

2015 ◽  
Vol 6 (1) ◽  
pp. 17-22
Author(s):  
A. I. Shevtsova ◽  
G. A. Ushakova
Keyword(s):  
Blood Plasma ◽  
Total Protein ◽  
Protein Concentration ◽  
Male Rats ◽  
Antitumor Action ◽  
Total Protein Concentration ◽  
Monospecific Antibodies ◽  
Anthracycline Chemotherapy ◽  
In Blood Plasma

There is no standard as to treatment of anthracycline chemotherapy complications. The reduction of cytotoxic drugs toxicity without weakening of their antitumor action remains relevant. The extracellular matrix which key component is fibronectin is present in all tissues and it continuously undergoes controlled remodeling. So, the purpose of our work was to study the level of fibronectin in the experimental model of doxorubicin-induced cardiomyopathy and effects of this cytostatic and its co-administration with antioxidants of different nature.The level of fibronectin was measured by ELISA using monospecific antibodies against fibronectin (Sigma, USA), secondary anti-IgG labeled with horseradish peroxidase (Sigma, USA) and fibronectin standard (Sigma, USA). The study was conducted on Wistar male rats with weight of 210 ± 50 g which were divided into 4 groups by 8 animals in each group: 1 – control, rats receiving saline i/p; 2 – doxorubicin 1 mg/kg i/p once a week during 4 weeks; 3 – doxorubicin by the same scheme plus 1% 2-oxoglutarate in drinking water during 4 weeks;4 – doxorubicin by the same scheme and korvitin injection 30 min before doxorubicin application once a week during 4 weeks. Obtained data indicate the effect of doxorubicin to decrease in index mass heart in 38% of animals compared to control animals; decrease in total protein concentration by 8% (Р < 0,05) and increase of the level of fibronectin by 67% (P < 0,001) in blood plasma of rats and decrease in the level of fibronectin in the heart extract by 19% (Р < 0,05) under development of doxorubicin-induced cardiotoxicity. Increased fibronectin concentration in blood plasma had strong correlation with decreased total protein concentration in blood (r=0,80) and heart extract (r=0,59) in rats with doxorubicin-induced cardiomiophaty indicating the sensitive reaction of fibronectin to development of metabolic disorders under doxorubicin influence. 

Assay of cerebrospinal fluid protein: a rate biuret method evaluated.

1983 ◽  
Vol 29 (1) ◽  
pp. 126-129 ◽  
Author(s):  
P R Finley ◽  
R J Williams
Keyword(s):  
Cerebrospinal Fluid ◽  
Total Protein ◽  
Protein Concentration ◽  
Protein A ◽  
Colorimetric Method ◽  
Total Protein Concentration ◽  
Reaction Cell ◽  
Biuret Method ◽  
Automated Instrument ◽  
Cerebrospinal Fluid Protein

Abstract We evaluated a rate colorimetric method (Beckman) for measuring total protein in cerebrospinal fluid. The automated instrument we used was Beckman's ASTRA TM. A 100-microL sample of spinal fluid is introduced into the biuret reagent in the reaction cell and the increase in absorbance at 545 nm is monitored for 20.5 s. Solid-state circuits determine the rate of alkaline biuret-protein chelate formation, which is directly proportional to the total protein concentration in the sample. The linear range of measurement is 120 to 7500 mg/L. Day-to-day precision (CV) over the range of 150 to 1200 mg/L ranged from 15.2 to 2.3%. The method was unaffected by radical alteration of the albumin/globulin ratio, but there is a positive interference in the presence of hemoglobin, a suppression in the presence of bilirubin, and no effect by xanthochromia. The method is precise, accurate, rapid, and convenient. The method was compared with the trichloroacetic acid method as performed on the Du Pont aca III, giving a correlation coefficient (r2) of 0.9693. The method is precise, accurate, rapid, and convenient.

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