LARGE-SCALE CONTINUOUS FLOW EXPOSURE SYSTEMS FOR STUDYING THE FATE AND EFFECTS OF CHEMICALLY AND PHYSICALLY DISPERSED OIL ON BENTHIC MARINE COMMUNITIES

1985 ◽  
Vol 1985 (1) ◽  
pp. 641-641
Author(s):  
Robert Scott Carr ◽  
Jerry M. Neff ◽  
Paul D. Boehm
Keyword(s):  
Continuous Flow ◽  
Large Scale ◽  
Marine Communities ◽  
Dispersed Oil ◽  
Flow Exposure

Optimized Large Scale Synthesis of Phosphorothioate Oligonucleotides on PS-PEG Supports

1997 ◽  
Vol 52 (1) ◽  
pp. 110-116
Author(s):  
Michael Gerster ◽  
Martin Maier ◽  
Nils Clausen ◽  
Jens Schewitz ◽  
Ernst Bayer
Keyword(s):  
Gel Electrophoresis ◽  
Continuous Flow ◽  
Large Scale ◽  
Electrospray Mass Spectrometry ◽  
Small Scale ◽  
Batch Mode ◽  
Crucial Step ◽  
Capillary Gel Electrophoresis ◽  
Phosphoramidite Chemistry ◽  
Large Scale Synthesis

Sulphurization is a crucial step during synthesis of phosphorothioate oligonucleotides. Insufficient reaction leads to inhomogeneous products with phosphodiester defects and subsequently to destabilization of the oligomers in biological media. To achieve a maximum extent of sulphur incorporation, various sulphurizing agents have been investigated. Solely, the use of Beaucage reagent provided satisfactory results on PS-PEG supports. Based on our investigations in small scale synthesis (1 μmol) with continuous-flow technique, upscaling to the 0.1-0.25 mmolar range has been achieved using a peptide synthesizer. The syntheses were performed in batch mode with standard phosphoramidite chemistry. Additionally, large scale synthesis of a phosphodiester oligonucleotide has been carried out on PS-PEG with optimized protocols and compared to small scale synthesis on different supports. Products were analysed by 31P NMR, capillary gel electrophoresis and electrospray mass spectrometry. An extent of sulphurization of 99% and coupling effiencies of more than 99% were obtained and the products proved to have similar purity compared to small scale syntheses on CPG

Enzyme Fractionation by Means of Large- Scale Continuous- Flow Electrophoresis

1966 ◽  
Vol 12 (8) ◽  
pp. 497-504 ◽  
Author(s):  
Seymour Winsten ◽  
Jeanne Jackson ◽  
Paula Wolf
Keyword(s):  
Lactic Acid ◽  
Enzyme Activities ◽  
Continuous Flow ◽  
Large Scale ◽  
Leucine Aminopeptidase ◽  
Ph Effect ◽  
Good Recovery ◽  
Acid Dehydrogenase ◽  
Aldolase Activity ◽  
Single Serum Sample

Abstract Large- scale, microglass bead, continuous- flow electrophoresis was used to separateand collect protein fractions from a single serum sample. These fractions were assayed for a multiplicity of enzyme activities. Relatively good recovery was obtained with lactic acid dehydrogenase, leucine aminopeptidase, and phosphohexoseisomerase. Artifactually high recoveries were observed with aldotase and isocitric acid dehydrogenase. The increase observed in aldolase activity may be due to a pH effect.

scholarly journals Synthesis by extrusion: continuous, large-scale preparation of MOFs using little or no solvent

2015 ◽  
Vol 6 (3) ◽  
pp. 1645-1649 ◽  
Author(s):  
Deborah Crawford ◽  
José Casaban ◽  
Robert Haydon ◽  
Nicola Giri ◽  
Tony McNally ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Large Scale ◽  
Phase Synthesis ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Melt Phase

Continuous flow mechanochemical and melt-phase synthesis at kg h−1 rates from solid reagents and either no solvent, or only minimal solvent, is reported.

Continuous flow, large‐scale, microbial fuel cell system for the sustained treatment of swine waste

2019 ◽  
Vol 92 (1) ◽  
pp. 60-72 ◽  
Author(s):  
Sofia Babanova ◽  
Jason Jones ◽  
Sujal Phadke ◽  
Mengqian Lu ◽  
Carlo Angulo ◽  
...  
Keyword(s):  
Fuel Cell ◽  
Microbial Fuel Cell ◽  
Continuous Flow ◽  
Large Scale ◽  
Cell System ◽  
Fuel Cell System ◽  
Swine Waste

scholarly journals Composites with alginate beads: A novel design of nano-adsorbents impregnation for large-scale continuous flow wastewater treatment pilots

2020 ◽  
Vol 27 (10) ◽  
pp. 2499-2508 ◽  
Author(s):  
Faissal Aziz ◽  
Mounir El Achaby ◽  
Amina Lissaneddine ◽  
Khalid Aziz ◽  
Naaila Ouazzani ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Continuous Flow ◽  
Large Scale ◽  
Alginate Beads ◽  
Novel Design
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