Comparative Phytochemical Screening and In vitro Evaluation of Biological Activities between Aqueous and Ethanolic Extract of Momordica charantia L. Fruits

Combretum adenogonium is beniniens pharmacopoeia medicinal plant used for the treatment of various diseases. This work aims to study the phytochemical and assess some biological activities of C. adenogonium leaves extracts. The phytochemical analysis (qualitative et quantitative) was conducted by standard analytical chemistry method. Antioxydant activity was evaluated by the DPPH method. Antibacterial activity was evaluated in vitro with 10 references strains , 10 Staphylococcus strains isolated from European Scientific Journal October 2017 edition Vol.13, No.30 ISSN: 1857 – 7881 (Print) e - ISSN 1857- 7431 359 meat products and 10 clinical Staphylococcus aureus strains isolated from Buruli ulcer lesions and pus. The Minimum Inhibitory Concentration (MIC) and Bactericidal (CMB) were determined by macrodilution method. The extracts cytotoxic effect was evaluated with Artemia salina larvae. The phytochemical screening revealed the presence of flavonoids, tannins, anthocyanins, saponin and triterpenoids. The methanolique extract present the higest content (450.66 ± 0.004 µg EAG/mg) of total polyphenolic compound. The results showed the good antioxidant activity. The inhibitory diameter zone vary (p < 0.001) according to the strains. The largest medium inhibitory diameter (21.85 ± 0.17 mm) was obtained with the ethanolic extract, while the lowest (6.00 ±0.00 mm) were recorded with water-ethanol extract. The variation between CMI and CMB is not significant (p > 0.05). The higher LD50 (27.66 mg/ml) was recorded with methanol extract. The results of this study confirm some use of C. Adenogonium extracts. But; these extracts must be use with moderation

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Phytochemical screening & in-vitro evaluation of anti-inflammatory activity of clerodendrum indicum roots

World Journal of Current Medical and Pharmaceutical Research ◽

10.37022/wjcmpr.v3i6.201 ◽

2021 ◽

pp. 140-143

Author(s):

Sushma M ◽

Lahari S ◽

Mounika A ◽

Sailaja K.E

Keyword(s):

Ethanolic Extract ◽

Inflammatory Activity ◽

Phytochemical Screening ◽

In Vitro Evaluation ◽

Anti Cancer ◽

The Family ◽

Steroid Glycosides ◽

Anti Oxidant ◽

Anti Inflammatory

This study aims to extract the phytochemical constituents and in-vitro evaluation of anti-inflammatory activity of roots of clerodendrum Indicum. Clerodendrum indicum (L.) kuntz. Commonly known as bharangi or chingari, belongs to the family verbenaceae. The plant is expectorant, stomachic, Anti-bronchitis, Anti-nociceptive, Anti-microbial, Anti-diarrheal, anti-oxidant and anti-cancer. Preliminary phytochemical screening revealed the presence of flavonoids, steroids, steroid glycosides, terpenoids etc. This study has taken with the Anti-inflammatory activity and preliminary phytochemical screening on the clerodendrum indicum. In the present study, qualitative study of the phytochemicals present in the sample was determined, Anti-inflammatory activity were carried out. On the premise of the prevailing study, the outcomes eventually concluded that the ethanolic extract of clerodendrum indicum own anti inflammatory pastime. This evaluation offers an concept that the compound of the plant clerodendrum indicum may be used as lead compound for designing robust pills which may be used for remedy of diverse diseases.

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Biological Activities of Extracts from Ageratum fastigiatum: Phytochemical Study and In Silico Target Fishing Approach

Planta Medica ◽

10.1055/a-1576-4080 ◽

2021 ◽

Author(s):

Carolina Reis Cerqueira Sudan ◽

Lucas Campos Pereira ◽

Andréia Fonseca Silva ◽

Carolina Paula de Souza Moreira ◽

Denise de Oliveira Scoaris ◽

...

Keyword(s):

In Silico ◽

Biological Activities ◽

Ethanolic Extract ◽

Antimicrobial Activities ◽

Biological Properties ◽

Preparative Chromatography ◽

Phytochemical Screening ◽

Phytochemical Study ◽

Target Fishing

AbstractIn the present study, the ethanolic extract from aerial parts of Ageratum fastigiatum was evaluated in vitro against epimastigote forms of Trypanosoma cruzi (Y strain), promastigote forms of Leishmania amazonensis (PH8 strain), and L. chagasi (BH400 strain). The extract was also evaluated against Staphylococcus aureus (ATCC 25 923), Escherichia coli (ATCC 11 775), Pseudomonas aeruginosa (ATCC 10 145), and Candida albicans (ATCC 36 802). The phytochemical screening was performed by thin-layer chromatography and high-performance liquid chromatography. The extract was fractionated using flash preparative chromatography. The ethanolic extract showed activity against T. cruzi, L. chagasi, and L. amazonensis and antimicrobial activity against S. aureus, E. coli, P. aeruginosa, and C. albicans. The phytochemical screening revealed coumarins, terpenes/sterols, and flavonoids in the ethanolic extract. In addition, the coumarin identified as ayapin was isolated from this extract. We also performed in silico prediction of potential biological activities and targets for compounds previously found in A. fastigiatum. Several predictions were confirmed both retrospectively and prospectively by experimental results described here or elsewhere. Some activities described in the in silico target fishing approach were validated by the ethnopharmacological use and known biological properties. Some new activities and/or targets were predicted and could guide future studies. These results suggest that A. fastigiatum can be an interesting source of substances with antiparasitic and antimicrobial activities.

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PRELIMINARY PHYTOCHEMICAL SCREENING, GC-MS PROFILING AND IN VITRO EVALUATION OF BIOLOGICAL ACTIVITIES OF GARCINIA ATROVIRIDIS ROOT EXTRACTS.

International Journal of Advanced Research ◽

10.21474/ijar01/8295 ◽

2018 ◽

Vol 7 (1) ◽

pp. 53-61 ◽

Cited By ~ 1

Author(s):

Nur SalsabilaAhmadRoslan ◽

◽

Seema Zareen ◽

Normaiza Zamri ◽

Muhammad NadeemAkhtar ◽

...

Keyword(s):

Biological Activities ◽

Phytochemical Screening ◽

In Vitro Evaluation ◽

Root Extracts

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In-Vitro Studies on the Sensitivity of Staphylococcus aureus to Some Ethno-Medicinal Preparations Sold around Kano, Nigeria

Bayero Journal of Pure and Applied Sciences ◽

10.4314/bajopas.v4i1.4 ◽

2011 ◽

Vol 4 (1) ◽

pp. 22-25 ◽

Cited By ~ 1

Author(s):

M Bashir ◽

I Yusuf ◽

AS Kutama

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Minimum Inhibitory Concentration ◽

Inhibitory Concentration ◽

Test Organism ◽

Ethanolic Extract ◽

Screening Tests ◽

Phytochemical Screening ◽

Herbal Preparations

Five traditional herbal preparations were sampled between May-June, 2009 in Kano. The samples were investigated for invitro antibacterial activities against clinical isolates of Staphylococcus aureus. Likewise, phytochemical screening tests were conducted to determine some of the phytochemicals present in the ethanolic and water extracts of the samples. Various concentrations of the extracts were prepared using serial doubling dilutions (5000=l/ml, 2500=g/ml, 1250=g/ml, 625=g/ml and 312.5=g/ml). All the test extracts showed slight antibacterial activity against the test organism, with ethanolic extract of sample E having the highest zone diameter of inhibition, while sample H had the lowest diameter of inhibition. The standard antibiotic disc (Gentamicin) had demonstrated the highest activity on the test organisms. The results of the Phytochemical screening revealed the presence of steroid in all the samples, tannin in samples A, C, D and E, reducing sugars in sample A, D and E respectively. The result of the minimum inhibitory concentration (MIC) was found to be above 312.5=g/ml for samples C, D and E. Keywords: Staphylococcus aureus, Herbal preparations, antibacterial activity, Phytochemical screening and minimum inhibitory concentration.

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