Detection of CPV 2a Antigen Type of Canine Parvovirus in the States of Punjab and Assam, India

Aim: Canine Parvovirus (CPV) is an emerging and re-emerging virus of canines. The study was undertaken to analyze VP2 gene of CPV in the isolates from dogs positive for CPV infection. Methods: The rectal swabs were collected from dogs suspected of CPV and subjected to PCR and nested PCR. The regions compared in the study were Punjab to represent north part of India and Assam to represent north-eastern part of India. The sequence analysis of VP2 gene of CPV was done using NCBI BLAST from the isolates which were positive for CPV by nested PCR. Further, phylogenetic analysis was done to understand the prevailing antigenic type of CPV from northern and north eastern part of India. Results: The sequence analysis revealed that all the sequences of VP2 gene from the samples had 98-99% homology with Canine Parvovirus and phylogenetic analysis revealed that CPV 2a antigenic type is circulating in both the regions selected in the study. Conclusions: The present study revealed that CPV 2a is circulating in the regions of Punjab and Assam part of India. Further sequence analysis of VP2 gene from more number of field isolates can throw better light on the prevailing antigenic type of CPV in various parts of India.

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Prevalence and molecular characterization of canine parvovirus

Veterinary World ◽

10.14202/vetworld.2021.603-606 ◽

2021 ◽

Vol 14 (3) ◽

pp. 603-606

Author(s):

Parikshit Singh ◽

Gurpreet Kaur ◽

Mudit Chandra ◽

P. N. Dwivedi

Keyword(s):

Phylogenetic Analysis ◽

Sequence Analysis ◽

Clinical Signs ◽

Canine Parvovirus ◽

Vp2 Gene ◽

Northern India ◽

Percent Positivity ◽

Northern Regions ◽

Vaccination History ◽

Antigenic Type

Background and Aim: Canine parvovirus (CPV) belonging to family Parvoviridae causes hemorrhagic gastroenteritis in dogs and heavy mortality in young dogs. The virus has three structural (VP1, VP2 and VP3) and two non-structural proteins (NS1 and NS2), VP2 being highly immunogenic. This study aims to study molecular epidemiology of CPV by sequence analysis of VP2 gene to determine the prevailing antigenic type(s) in the northern regions of India. Materials and Methods: A total of 118 rectal swabs collected from dogs exhibiting clinical signs of CPV infection were processed for the isolation of DNA and subjected to polymerase chain reaction (PCR) and nested PCR (NPCR). A total of 13 NPCR products selected randomly were subjected to sequence analysis of VP2 gene. Results: The percent positivity of CPV was found 28% and 70% by PCR and NPCR, respectively. Dogs with vaccination history against CPV too were found positive with a percent positivity of 24.10%. Gene sequencing and phylogenetic analysis of VP2 gene from these isolates revealed that most samples formed a clade with CPV-2a isolates. Conclusion: Sequence analysis and phylogenetic analysis of VP2 gene in the studied regions of northern India revealed that CPV-2a was the most prevalent antigenic type.

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Phylogenetic analysis of canine parvovirus partial VP2 gene in India

Virus Genes ◽

10.1007/s11262-013-1000-5 ◽

2013 ◽

Vol 48 (1) ◽

pp. 89-95 ◽

Cited By ~ 19

Author(s):

H. K. Mukhopadhyay ◽

Samyukta Lakshmi Matta ◽

S. Amsaveni ◽

P. X. Antony ◽

J. Thanislass ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Canine Parvovirus ◽

Vp2 Gene

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Prevalence and Phylogenetic Analysis of Microsporidium Enterocytozoon bieneusi in Diarrheal Patients

Pathogens ◽

10.3390/pathogens10020128 ◽

2021 ◽

Vol 10 (2) ◽

pp. 128

Author(s):

Manman Zang ◽

Jinjin Li ◽

Chun Tang ◽

Songtao Ding ◽

Wei Huang ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Sequence Analysis ◽

Molecular Epidemiology ◽

Epidemiological Study ◽

Microscopic Examination ◽

Nested Pcr ◽

Its Region ◽

Enterocytozoon Bieneusi ◽

Severe Diarrhea ◽

And Control

Enterocytozoon bieneusi can cause severe diarrhea in children and adults. However, in China, there are scant studies on E. bieneusi in diarrheal children and adults, with the exception of prevalence and genotyping data in a small number of cities including Hubei, Shanghai, and Heilongjiang. In this study, 196 fecal samples (n = 132 in Chongqing, n = 44 in Shandong, n = 20 in Hubei) were collected, including 91 from children and 105 from adults. Through microscopic examination, 19 positive samples (11 from children and 8 from adults) were detected. Using PCR examination, the internal transcriptional spacer (ITS) region was utilized by nested PCR to detect and characterize E. bieneusi. Twenty positive samples were detected, including 14 from children (≤11 years of age) and 6 from adults. According to the sequence analysis of ITS data, one known zoonotic (D) and seven novel (CQH5-11) genotypes were identified. This is the first molecular epidemiological study of E. bieneusi in diarrheal patients in different regions of China. Therefore, this study can provide useful information for the molecular epidemiology and control of E. bieneusi infection in humans in the future.

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Sequence analysis of VP2 gene of canine parvovirus isolated from domestic dogs in Japan in 1999 and 2000

Comparative Immunology Microbiology and Infectious Diseases ◽

10.1016/j.cimid.2006.05.001 ◽

2006 ◽

Vol 29 (4) ◽

pp. 199-206 ◽

Cited By ~ 18

Author(s):

M DOKI ◽

K FUJITA ◽

R MIURA ◽

M YONEDA ◽

Y ISHIKAWA ◽

...

Keyword(s):

Sequence Analysis ◽

Canine Parvovirus ◽

Domestic Dogs ◽

Vp2 Gene

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Phylogenetic Analysis of Canine Parvovirus VP2 Gene in Taiwan

Virus Genes ◽

10.1007/s11262-005-1791-0 ◽

2005 ◽

Vol 31 (2) ◽

pp. 171-174 ◽

Cited By ~ 35

Author(s):

Hsien-Chi Wang ◽

Wei-Da Chen ◽

Shiun-Long Lin ◽

Jacky Peng-Wen Chan ◽

Min-Liang Wong

Keyword(s):

Phylogenetic Analysis ◽

Canine Parvovirus ◽

Vp2 Gene

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Phylogenetic analysis of VP2 gene of canine parvovirus and comparison with Indian and world isolates

Acta Virologica ◽

10.4149/av_2016_01_106 ◽

2016 ◽

Vol 60 (01) ◽

pp. 106-110 ◽

Cited By ~ 1

Author(s):

G. KAUR ◽

M. CHANDRA ◽

P. N. DWIVEDI

Keyword(s):

Phylogenetic Analysis ◽

Canine Parvovirus ◽

Vp2 Gene

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Molecular typing of canine parvovirus from Sulaimani, Iraq and phylogenetic analysis using partial VP2 gene

BULGARIAN JOURNAL OF VETERINARY MEDICINE ◽

10.15547/bjvm.1032 ◽

2017 ◽

Vol 20 (3) ◽

pp. 225-235 ◽

Cited By ~ 1

Author(s):

M. Baba Sheikh ◽

P. Rashid ◽

A. Marouf ◽

Z. Raheem ◽

S. Manjunath ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Molecular Typing ◽

Canine Parvovirus ◽

Vp2 Gene

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Genotyping and phylogenetic analysis of canine parvovirus circulating in Egypt

Veterinary World ◽

10.14202/vetworld.2020.326-333 ◽

2020 ◽

Vol 13 (2) ◽

pp. 326-333 ◽

Cited By ~ 1

Author(s):

Kawther Sayed Zaher ◽

Wahid Hussein El-Dabae ◽

Mostafa Mohamed El-Sebelgy ◽

Naglaa Ibrahim Aly ◽

Zeinab Taha Salama

Keyword(s):

Phylogenetic Analysis ◽

Mdck Cells ◽

Rapid Test ◽

Epidemiological Studies ◽

Canine Parvovirus ◽

Vp2 Gene ◽

Test Kit ◽

Polymerase Chain ◽

Taiwanese Strain ◽

Vietnamese Strain

Aim: This study aimed to detect and characterize current genotypes of canine parvovirus (CPV) in Egypt during 2018. Materials and Methods: A total of 50 fecal swabs were collected from clinically infected domestic dogs of 2-5 months of age, suspected to suffer from CPV infection, from Cairo and Giza Governorates. The samples were subjected to qualitative antigen detection using the rapid test, followed by isolation on Madin-Darby Canine Kidney (MDCK) cells, molecular characterization with partial amplification of VP2 gene using polymerase chain reaction (PCR), followed by sequencing and phylogenetic analysis. Results: Out of 50 fecal samples, 20 samples were positive (40%) by Rapid CPV/canine coronavirus Ag Test Kit. These positive samples were cultured successfully on MDCK cells. Nine randomly chosen samples out of 30 apparently negative samples were amplified using PCR with primers Hfor and Hrev to yield a typical 630 bp fragment. Then, six randomly chosen samples out of nine were amplified using PCR with primers Pbs and Pbas to yield a typical 427 bp fragment. Sequencing, BLAST analysis and assembly of the two fragments (630 bp and 427 bp) to produce 912 bp fragments, in the six samples, revealed two serotypes CPV-2b and CPV-2c. The obtained strains were submitted to GenBank and given accession numbers MK642272, MK642273, MK642274, MK642275, MK642276, and MK642277. Phylogenetic analysis of the Egyptian strains serotype 2b illustrated that they were closely related to Thailand strains (accession numbers KP715709, KP715694, KP715701, and KP715700); while Egyptian strains serotype 2c was closely related to Thailand strains (accession numbers MH711894 and MH711902), Taiwanese strain (KU244254), Chinese strain (MF467242), and Vietnamese strain (accession number LC216910). Conclusion: The current research recommends further epidemiological studies to assess the extent of the occurrence of different serotypes of CPV in Egypt and the efficiency of imported and locally produced vaccines in protection against CPV infection.

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