scholarly journals Prevalence and molecular characterization of canine parvovirus

2021 ◽  
Vol 14 (3) ◽  
pp. 603-606
Author(s):  
Parikshit Singh ◽  
Gurpreet Kaur ◽  
Mudit Chandra ◽  
P. N. Dwivedi
Keyword(s):  
Phylogenetic Analysis ◽  
Sequence Analysis ◽  
Clinical Signs ◽  
Canine Parvovirus ◽  
Vp2 Gene ◽  
Northern India ◽  
Percent Positivity ◽  
Northern Regions ◽  
Vaccination History ◽  
Antigenic Type

Background and Aim: Canine parvovirus (CPV) belonging to family Parvoviridae causes hemorrhagic gastroenteritis in dogs and heavy mortality in young dogs. The virus has three structural (VP1, VP2 and VP3) and two non-structural proteins (NS1 and NS2), VP2 being highly immunogenic. This study aims to study molecular epidemiology of CPV by sequence analysis of VP2 gene to determine the prevailing antigenic type(s) in the northern regions of India. Materials and Methods: A total of 118 rectal swabs collected from dogs exhibiting clinical signs of CPV infection were processed for the isolation of DNA and subjected to polymerase chain reaction (PCR) and nested PCR (NPCR). A total of 13 NPCR products selected randomly were subjected to sequence analysis of VP2 gene. Results: The percent positivity of CPV was found 28% and 70% by PCR and NPCR, respectively. Dogs with vaccination history against CPV too were found positive with a percent positivity of 24.10%. Gene sequencing and phylogenetic analysis of VP2 gene from these isolates revealed that most samples formed a clade with CPV-2a isolates. Conclusion: Sequence analysis and phylogenetic analysis of VP2 gene in the studied regions of northern India revealed that CPV-2a was the most prevalent antigenic type.

scholarly journals Detection of CPV 2a Antigen Type of Canine Parvovirus in the States of Punjab and Assam, India

2019 ◽  
pp. 1-7
Author(s):  
Himasri Das ◽  
Gurpreet Kaur ◽  
Mudit Chandra ◽  
P. N. Dwivedi
Keyword(s):  
Phylogenetic Analysis ◽  
Sequence Analysis ◽  
Nested Pcr ◽  
Canine Parvovirus ◽  
Vp2 Gene ◽  
North Eastern ◽  
North Part ◽  
Emerging Virus ◽  
Antigenic Type ◽  
North Eastern Part

Aim: Canine Parvovirus (CPV) is an emerging and re-emerging virus of canines. The study was undertaken to analyze VP2 gene of CPV in the isolates from dogs positive for CPV infection. Methods: The rectal swabs were collected from dogs suspected of CPV and subjected to PCR and nested PCR. The regions compared in the study were Punjab to represent north part of India and Assam to represent north-eastern part of India. The sequence analysis of VP2 gene of CPV was done using NCBI BLAST from the isolates which were positive for CPV by nested PCR. Further, phylogenetic analysis was done to understand the prevailing antigenic type of CPV from northern and north eastern part of India. Results: The sequence analysis revealed that all the sequences of VP2 gene from the samples had 98-99% homology with Canine Parvovirus and phylogenetic analysis revealed that CPV 2a antigenic type is circulating in both the regions selected in the study. Conclusions: The present study revealed that CPV 2a is circulating in the regions of Punjab and Assam part of India. Further sequence analysis of VP2 gene from more number of field isolates can throw better light on the prevailing antigenic type of CPV in various parts of India.

Phylogenetic analysis of canine parvovirus partial VP2 gene in India

Virus Genes ◽  
2013 ◽  
Vol 48 (1) ◽  
pp. 89-95 ◽  
Author(s):  
H. K. Mukhopadhyay ◽  
Samyukta Lakshmi Matta ◽  
S. Amsaveni ◽  
P. X. Antony ◽  
J. Thanislass ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Canine Parvovirus ◽  
Vp2 Gene

scholarly journals Dynamic evolution of canine parvovirus in Thailand

2020 ◽  
Vol 13 (2) ◽  
pp. 245-255
Author(s):  
N. Inthong ◽  
S. Kaewmongkol ◽  
N. Meekhanon ◽  
K. Sirinarumitr ◽  
T. Sirinarumitr
Keyword(s):  
Amino Acids ◽  
Clinical Signs ◽  
Canine Parvovirus ◽  
Specific Primers ◽  
Highly Pathogenic ◽  
Vp2 Gene ◽  
Fecal Samples ◽  
Feline Panleukopenia Virus ◽  
Close Relationship ◽  
Polymerase Chain

Background and Aim: According to the previous study, the circulating canine parvovirus (CPV) in Thailand is 2a and 2b. Nowadays, CPV mutants, including CPV-2c, have been identified in many parts of the world. This study aimed to investigate the genetic diversity of the circulating CPV in Thailand. Materials and Methods: Eighty-five CPV-positive fecal samples were obtained from dogs with either acute hemorrhagic diarrhea or diarrhea. The complete VP2 gene of these samples was amplified using VP2 specific primers and polymerase chain reaction (PCR). The obtained full-length VP2 sequences were analyzed and a phylogenetic tree was constructed. Results: Sixty and 25 CPV-positive fecal samples were collected in 2010 and 2018, respectively. Thirty-four samples were new CPV-2a and 31 samples were new CPV-2b due to amino acids substitution at position 297 (Ser-Ala). In 2018, 5 new CPV-2a, 19 CPV-2c, and 1 feline panleukopenia virus (FPV) were found, but no new CPV-2b was detected. Moreover, most of the CPV in this study had amino acids mutations at positions 324 and 440. The phylogenetic construction demonstrated the close relationship between the current new CPV-2a with the previous CPV-2a reported from Thailand, China, Uruguay, Vietnam, Singapore, and India. Interestingly, the current new CPV-2b in this study was not closely related to the previous CPV-2b reported in Thailand. The CPV-2c in this study was closer to Asian CPV-2c and further from either European or South America CPV-2c. Interestingly, FPV was identified in a diarrhea dog. Conclusion: The evolution of CPV in Thailand is very dynamic. Thus, it is important to monitor for CPV mutants and especially the clinical signs relating to these mutants to conduct surveillance for the emergence of new highly pathogenic CPV in the future.

Sequence analysis of VP2 gene of canine parvovirus isolated from domestic dogs in Japan in 1999 and 2000

2006 ◽  
Vol 29 (4) ◽  
pp. 199-206 ◽  
Author(s):  
M DOKI ◽  
K FUJITA ◽  
R MIURA ◽  
M YONEDA ◽  
Y ISHIKAWA ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Canine Parvovirus ◽  
Domestic Dogs ◽  
Vp2 Gene

Phylogenetic Analysis of Canine Parvovirus VP2 Gene in Taiwan

Virus Genes ◽  
2005 ◽  
Vol 31 (2) ◽  
pp. 171-174 ◽  
Author(s):  
Hsien-Chi Wang ◽  
Wei-Da Chen ◽  
Shiun-Long Lin ◽  
Jacky Peng-Wen Chan ◽  
Min-Liang Wong
Keyword(s):  
Phylogenetic Analysis ◽  
Canine Parvovirus ◽  
Vp2 Gene

scholarly journals The detection of canine parvovirus type 2c of Asian origin in dogs in Romania evidenced its progressive worldwide diffusion

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Andrea Balboni ◽  
Mihaela Niculae ◽  
Serena Di Vito ◽  
Lorenza Urbani ◽  
Alessia Terrusi ◽  
...  
Keyword(s):  
Amino Acid ◽  
Acute Gastroenteritis ◽  
Clinical Signs ◽  
Canine Parvovirus ◽  
Amino Acid Residues ◽  
Asian Origin ◽  
Vp2 Gene ◽  
Faecal Samples ◽  
Canine Population ◽  
Pcr Targeting

Abstract Background Canine parvovirus (CPV) is one of the most important pathogens of dogs. Despite vaccination, CPV infections are still ubiquitous in dogs, and the three antigenic variants 2a, 2b and 2c are variously distributed in the canine population worldwide. To date, no information is available on CPV variants circulating in some European countries. The aim of this study was to genetically characterise the CPV detected in ten dogs with clinical signs of acute gastroenteritis in Romania. The presence of Carnivore protoparvovirus 1 DNA was investigated in faecal samples using an end-point PCR targeting the complete VP2 gene and positive amplicons were sequenced and analysed. Results All ten dogs with acute gastroenteritis tested positive to Carnivore protoparvovirus 1 DNA in faecal samples. The identified viruses belonged to CPV-2c type, showed identical sequences of the VP2 gene and were characterised by distinctive amino acid residues in the deduced VP2 protein: 5-glicine (5Gly), 267-tirosine (267Tyr), 324-isoleucine (324Ile) and 370-arginine (370Arg). These distinctive amino acid residues have already been reported in CPV-2c widespread in Asia and occasionally detected in Italy and Nigeria. Conclusions Since CPV-2c with VP2 amino acid residues 5Gly, 267Tyr, 324Ile and 370Arg were never reported before 2013, it can be assumed that this virus is progressively expanding its spread in the world dog population. This study adds new data about the presence of this new virus in Europe and underline worrying questions about its potential impact on the health of the canine population.

scholarly journals Molecular Analysis of Full-Length VP2 of Canine Parvovirus Reveals Antigenic Drift in CPV-2b and CPV-2c Variants in Central Chile

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2387
Author(s):  
Véliz-Ahumada Alexis ◽  
Vidal Sonia ◽  
Siel Daniela ◽  
Guzmán Miguel ◽  
Hardman Timothy ◽  
...  
Keyword(s):  
Amino Acid ◽  
Protective Immunity ◽  
Clinical Signs ◽  
Canine Parvovirus ◽  
Antigenic Drift ◽  
Vp2 Gene ◽  
Central Chile ◽  
Vp2 Protein

Canine parvovirus (CPV) is a major pathogen in canines, with a high mortality rate in unvaccinated puppies. CPV is traditionally classified into three antigenic variants (CPV-2a, CPV-2b and CPV-2c) based on the amino acid sequence of the VP2 protein. Currently, various mutations are described in the receptor-binding area or in the regions of greatest antigenicity of the VP2 protein, giving rise to new viral variants that are capable of immunological escape, affecting the protective immunity of traditional vaccines. In the present study, a molecular characterization of the VP2 gene was performed, which included phylogenetic analysis, amino acid characterization and determination of selection pressures. Blood samples were initially collected from canine patients with clinical signs of gastrointestinal infection, of which 69 were positive for CPV as measured by means of PCR and 18 samples were selected for the amplification of the complete VP2 gene. The analysis revealed a higher rate of CPV-2c-positive patients compared to CPV-2b. Furthermore, the amino acid characterization of VP2 indicated mutations in the regions of highest antigenicity previously described in the literature (CPV-2b: 297 and 324; CPV-2c: 440), as well as others not previously documented (CPV-2b: 514; CPV-2c: 188, 322, 379, 427 and 463). Our analysis of selection pressure showed that the VP2 gene is under negative selection. However, positive selection point sites were identified, both in CPV-2c (324, 426 and 440) and CPV-2b (297 and 324), at sites that have been associated with evasion of the immune response via antigenic drift, which possibly has implications for the protective immunity generated by traditional vaccines.

scholarly journals Phylogenetic analysis of VP2 gene of canine parvovirus and comparison with Indian and world isolates

2016 ◽  
Vol 60 (01) ◽  
pp. 106-110 ◽  
Author(s):  
G. KAUR ◽  
M. CHANDRA ◽  
P. N. DWIVEDI
Keyword(s):  
Phylogenetic Analysis ◽  
Canine Parvovirus ◽  
Vp2 Gene

scholarly journals Molecular typing of canine parvovirus from Sulaimani, Iraq and phylogenetic analysis using partial VP2 gene

2017 ◽  
Vol 20 (3) ◽  
pp. 225-235 ◽  
Author(s):  
M. Baba Sheikh ◽  
P. Rashid ◽  
A. Marouf ◽  
Z. Raheem ◽  
S. Manjunath ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Typing ◽  
Canine Parvovirus ◽  
Vp2 Gene

scholarly journals Genotyping and phylogenetic analysis of canine parvovirus circulating in Egypt

2020 ◽  
Vol 13 (2) ◽  
pp. 326-333 ◽  
Author(s):  
Kawther Sayed Zaher ◽  
Wahid Hussein El-Dabae ◽  
Mostafa Mohamed El-Sebelgy ◽  
Naglaa Ibrahim Aly ◽  
Zeinab Taha Salama
Keyword(s):  
Phylogenetic Analysis ◽  
Mdck Cells ◽  
Rapid Test ◽  
Epidemiological Studies ◽  
Canine Parvovirus ◽  
Vp2 Gene ◽  
Test Kit ◽  
Polymerase Chain ◽  
Taiwanese Strain ◽  
Vietnamese Strain

Aim: This study aimed to detect and characterize current genotypes of canine parvovirus (CPV) in Egypt during 2018. Materials and Methods: A total of 50 fecal swabs were collected from clinically infected domestic dogs of 2-5 months of age, suspected to suffer from CPV infection, from Cairo and Giza Governorates. The samples were subjected to qualitative antigen detection using the rapid test, followed by isolation on Madin-Darby Canine Kidney (MDCK) cells, molecular characterization with partial amplification of VP2 gene using polymerase chain reaction (PCR), followed by sequencing and phylogenetic analysis. Results: Out of 50 fecal samples, 20 samples were positive (40%) by Rapid CPV/canine coronavirus Ag Test Kit. These positive samples were cultured successfully on MDCK cells. Nine randomly chosen samples out of 30 apparently negative samples were amplified using PCR with primers Hfor and Hrev to yield a typical 630 bp fragment. Then, six randomly chosen samples out of nine were amplified using PCR with primers Pbs and Pbas to yield a typical 427 bp fragment. Sequencing, BLAST analysis and assembly of the two fragments (630 bp and 427 bp) to produce 912 bp fragments, in the six samples, revealed two serotypes CPV-2b and CPV-2c. The obtained strains were submitted to GenBank and given accession numbers MK642272, MK642273, MK642274, MK642275, MK642276, and MK642277. Phylogenetic analysis of the Egyptian strains serotype 2b illustrated that they were closely related to Thailand strains (accession numbers KP715709, KP715694, KP715701, and KP715700); while Egyptian strains serotype 2c was closely related to Thailand strains (accession numbers MH711894 and MH711902), Taiwanese strain (KU244254), Chinese strain (MF467242), and Vietnamese strain (accession number LC216910). Conclusion: The current research recommends further epidemiological studies to assess the extent of the occurrence of different serotypes of CPV in Egypt and the efficiency of imported and locally produced vaccines in protection against CPV infection.

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