Sequence analysis of VP2 gene of canine parvovirus isolated from domestic dogs in Japan in 1999 and 2000

Background and Aim: Canine parvovirus (CPV) belonging to family Parvoviridae causes hemorrhagic gastroenteritis in dogs and heavy mortality in young dogs. The virus has three structural (VP1, VP2 and VP3) and two non-structural proteins (NS1 and NS2), VP2 being highly immunogenic. This study aims to study molecular epidemiology of CPV by sequence analysis of VP2 gene to determine the prevailing antigenic type(s) in the northern regions of India. Materials and Methods: A total of 118 rectal swabs collected from dogs exhibiting clinical signs of CPV infection were processed for the isolation of DNA and subjected to polymerase chain reaction (PCR) and nested PCR (NPCR). A total of 13 NPCR products selected randomly were subjected to sequence analysis of VP2 gene. Results: The percent positivity of CPV was found 28% and 70% by PCR and NPCR, respectively. Dogs with vaccination history against CPV too were found positive with a percent positivity of 24.10%. Gene sequencing and phylogenetic analysis of VP2 gene from these isolates revealed that most samples formed a clade with CPV-2a isolates. Conclusion: Sequence analysis and phylogenetic analysis of VP2 gene in the studied regions of northern India revealed that CPV-2a was the most prevalent antigenic type.

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Detection of CPV 2a Antigen Type of Canine Parvovirus in the States of Punjab and Assam, India

Current Journal of Applied Science and Technology ◽

10.9734/cjast/2019/v38i230354 ◽

2019 ◽

pp. 1-7

Author(s):

Himasri Das ◽

Gurpreet Kaur ◽

Mudit Chandra ◽

P. N. Dwivedi

Keyword(s):

Phylogenetic Analysis ◽

Sequence Analysis ◽

Nested Pcr ◽

Canine Parvovirus ◽

Vp2 Gene ◽

North Eastern ◽

North Part ◽

Emerging Virus ◽

Antigenic Type ◽

North Eastern Part

Aim: Canine Parvovirus (CPV) is an emerging and re-emerging virus of canines. The study was undertaken to analyze VP2 gene of CPV in the isolates from dogs positive for CPV infection. Methods: The rectal swabs were collected from dogs suspected of CPV and subjected to PCR and nested PCR. The regions compared in the study were Punjab to represent north part of India and Assam to represent north-eastern part of India. The sequence analysis of VP2 gene of CPV was done using NCBI BLAST from the isolates which were positive for CPV by nested PCR. Further, phylogenetic analysis was done to understand the prevailing antigenic type of CPV from northern and north eastern part of India. Results: The sequence analysis revealed that all the sequences of VP2 gene from the samples had 98-99% homology with Canine Parvovirus and phylogenetic analysis revealed that CPV 2a antigenic type is circulating in both the regions selected in the study. Conclusions: The present study revealed that CPV 2a is circulating in the regions of Punjab and Assam part of India. Further sequence analysis of VP2 gene from more number of field isolates can throw better light on the prevailing antigenic type of CPV in various parts of India.

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Phylogenetic Similarity of the Canine Parvovirus Wild-Type Isolates on the Basis of VP1/VP2 Gene Fragment Sequence Analysis

Journal of Veterinary Medicine Series B ◽

10.1046/j.1439-0450.2002.00523.x ◽

2002 ◽

Vol 49 (3) ◽

pp. 142-145 ◽

Cited By ~ 2

Author(s):

K. RYPULA ◽

R. CHMIELEWSKI ◽

E. SMIELEWSKA-LOS ◽

S. KLIMENTOWSKI

Keyword(s):

Sequence Analysis ◽

Canine Parvovirus ◽

Gene Fragment ◽

Wild Type ◽

Vp2 Gene

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Molecular characterization of canine parvovirus variants (CPV-2a, CPV-2b, and CPV-2c) based on the VP2 gene in affected domestic dogs in Ecuador

Veterinary World ◽

10.14202/vetworld.2018.480-487 ◽

2018 ◽

Vol 11 (4) ◽

pp. 480-487 ◽

Cited By ~ 9

Author(s):

David De la Torre ◽

Eulalia Mafla ◽

Byron Puga ◽

Linda Erazo ◽

Claudete Astolfi-Ferreira ◽

...

Keyword(s):

Molecular Characterization ◽

Canine Parvovirus ◽

Domestic Dogs ◽

Vp2 Gene

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Sequence analysis of VP2 gene of canine parvovirus isolates in Thailand

Virus Genes ◽

10.1007/s11262-010-0475-6 ◽

2010 ◽

Vol 41 (1) ◽

pp. 23-29 ◽

Cited By ~ 26

Author(s):

Siriwadee Phromnoi ◽

Kaitkanoke Sirinarumitr ◽

Theerapol Sirinarumitr

Keyword(s):

Sequence Analysis ◽

Canine Parvovirus ◽

Vp2 Gene

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Cerebellar hypoplasia and dysplasia in a juvenile raccoon with parvoviral infection

Journal of Veterinary Diagnostic Investigation ◽

10.1177/1040638720912229 ◽

2020 ◽

Vol 32 (3) ◽

pp. 463-466 ◽

Cited By ~ 1

Author(s):

Arno Wünschmann ◽

Robert Lopez-Astacio ◽

Anibal G. Armien ◽

Colin R. Parrish

Keyword(s):

Granule Cells ◽

Nonstructural Protein ◽

Canine Parvovirus ◽

Cerebellar Hypoplasia ◽

Diagnostic Laboratory ◽

Vp2 Gene ◽

Nonstructural Protein 1 ◽

Cell Processes ◽

Ns1 Gene ◽

Pcr Targeting

A juvenile raccoon ( Procyon lotor) was submitted dead to the Minnesota Veterinary Diagnostic Laboratory for rabies testing without history. The animal had marked hypoplasia of the cerebellum. Histology demonstrated that most folia lacked granule cells and had randomly misplaced Purkinje cells. Immunohistochemistry revealed the presence of parvoviral antigen in a few neurons and cell processes. PCR targeting feline and canine parvovirus yielded a positive signal. Sequencing analyses from a fragment of the nonstructural protein 1 ( NS1) gene and a portion of the viral capsid protein 2 ( VP2) gene confirmed the presence of DNA of a recent canine parvovirus variant (CPV-2a–like virus) in the cerebellum. Our study provides evidence that (canine) parvovirus may be associated with cerebellar hypoplasia and dysplasia in raccoons, similar to the disease that occurs naturally and has been reproduced experimentally by feline parvoviral infection of pregnant cats, with subsequent intrauterine or neonatal infections of the offspring.

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Molecular Characteristics of Carnivore Protoparvovirus 1 With High Sequence Similarity Between Wild and Domestic Carnivores in Taiwan

10.20944/preprints202104.0600.v1 ◽

2021 ◽

Author(s):

Ai-Mei Chang ◽

Chen-Chih Chen

Keyword(s):

Dna Sequences ◽

Gastrointestinal Disease ◽

Sequence Similarity ◽

Domestic Dogs ◽

Molecular Characteristics ◽

High Sequence Similarity ◽

Vp2 Gene ◽

Pcr Screening ◽

Wild Carnivores ◽

Domestic Carnivores

Carnivore protoparvovirus 1 (CPPV-1) is a DNA virus causing gastrointestinal disease and immunosuppression in various terrestrial carnivores. Domestic dogs and cats are considered the primary CPPV-1 reservoirs. The habitat overlaps of wild carnivores and free-roaming dogs increases the threat of CPPV-1 transmission between them. This study explored the CPPV-1 distribution among wild carnivores through PCR screening and compared the DNA sequences of the partial capsid protein (VP2) between wild and domestic carnivores. In total, 181 samples were screened for the CPPV-1 VP2 gene, including 32 masked palm civets (Paguma larvata), 63 Chinese ferret badgers (Melogale moschata), and 86 crab-eating mongooses (Herpestes urva), from 2015 to 2019 in Taiwan. The average prevalence of CPPV-1 was 17.7% (32/181), with the highest prevalence in masked palm civets (37.5%). In addition, a masked palm civet was coinfected with two CPPV-1 strains. Among the 33 partial VP2 gene sequences, 23 were identical to sequences amplified from domestic dogs and cats in Asia and the remaining 10 were identified for the first time. This study demonstrated that CPPV-1 has circulated between domestic and wild carnivores in rural Taiwan. Therefore, further population control and health management of free-roaming domestic carnivores are recommended.

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A phylogenetic study of canine parvovirus type 2c in midwestern Brazil

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2013000200013 ◽

2013 ◽

Vol 33 (2) ◽

pp. 214-218 ◽

Cited By ~ 10

Author(s):

Danúbia S. Fontana ◽

Paulo Ricardo D. Rocha ◽

Raquel A.S. Cruz ◽

Letícya L. Lopes ◽

Andréia L.T. Melo ◽

...

Keyword(s):

Amino Acid ◽

Genetic Variability ◽

Dna Sequencing ◽

Canine Parvovirus ◽

Phylogenetic Study ◽

Vp2 Gene ◽

Hemorrhagic Enteritis ◽

Canine Parvovirus Type 2 ◽

Representative Samples

Since the late 1970s, canine parvovirus type 2 (CPV-2) has emerged as a causative agent of fatal severe acute hemorrhagic enteritis in dogs. To date, three antigenic types of CPV-2 were described worldwide (CPV-2a/b/c). This study was conducted to determine the variants of CPV-2 circulating in dogs from the Cuiabá Municipality in Midwestern Brazil. Out of 50 fecal samples, collected between 2009 and 2011, 27 tested positive for CPV-2. A 583 bp fragment of the VP2 gene was amplified by PCR, 13 representative samples were analyzed further by DNA sequencing. All strains were characterized as CPV-2c, displayed a low genetic variability although observed several amino acid substitution. These findings indicated that CPV-2c has been circulating in dogs from the Cuiabá Municipality in Midwestern Brazil.

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