Genotyping and phylogenetic analysis of canine parvovirus circulating in Egypt

Aim: This study aimed to detect and characterize current genotypes of canine parvovirus (CPV) in Egypt during 2018. Materials and Methods: A total of 50 fecal swabs were collected from clinically infected domestic dogs of 2-5 months of age, suspected to suffer from CPV infection, from Cairo and Giza Governorates. The samples were subjected to qualitative antigen detection using the rapid test, followed by isolation on Madin-Darby Canine Kidney (MDCK) cells, molecular characterization with partial amplification of VP2 gene using polymerase chain reaction (PCR), followed by sequencing and phylogenetic analysis. Results: Out of 50 fecal samples, 20 samples were positive (40%) by Rapid CPV/canine coronavirus Ag Test Kit. These positive samples were cultured successfully on MDCK cells. Nine randomly chosen samples out of 30 apparently negative samples were amplified using PCR with primers Hfor and Hrev to yield a typical 630 bp fragment. Then, six randomly chosen samples out of nine were amplified using PCR with primers Pbs and Pbas to yield a typical 427 bp fragment. Sequencing, BLAST analysis and assembly of the two fragments (630 bp and 427 bp) to produce 912 bp fragments, in the six samples, revealed two serotypes CPV-2b and CPV-2c. The obtained strains were submitted to GenBank and given accession numbers MK642272, MK642273, MK642274, MK642275, MK642276, and MK642277. Phylogenetic analysis of the Egyptian strains serotype 2b illustrated that they were closely related to Thailand strains (accession numbers KP715709, KP715694, KP715701, and KP715700); while Egyptian strains serotype 2c was closely related to Thailand strains (accession numbers MH711894 and MH711902), Taiwanese strain (KU244254), Chinese strain (MF467242), and Vietnamese strain (accession number LC216910). Conclusion: The current research recommends further epidemiological studies to assess the extent of the occurrence of different serotypes of CPV in Egypt and the efficiency of imported and locally produced vaccines in protection against CPV infection.

Download Full-text

Molecular characterization and phylogenetic analysis of a canine parvovirus isolate in India

Veterinární Medicína ◽

10.17221/147/2009-vetmed ◽

2009 ◽

Vol 54 (No. 10) ◽

pp. 483-490 ◽

Cited By ~ 5

Author(s):

S. Nandi ◽

S. Chidri ◽

M. Kumar

Keyword(s):

Phylogenetic Analysis ◽

Nucleotide Sequence ◽

Culture System ◽

Mdck Cells ◽

Canine Parvovirus ◽

Cell Culture System ◽

Chain Reaction ◽

Vp2 Gene ◽

Polymerase Chain ◽

Hemorrhagic Enteritis

Canine parvovirus 2 (CPV-2) is the causative agent of acute hemorrhagic enteritis and myocarditis in dogs. In this study the nucleotide sequence of the VP1/VP2 gene of a CPV isolate from India was analyzed and the phylogenetic relationship with other CPV isolates was established. Out of 36 samples analyzed, 16 were found positive for CPV-2 by polymerase chain reaction (PCR). Among the 16 positive samples, five were inoculated in MDCK cells for isolation out of which one adapted successfully to the cell culture system. Phylogenetic analysis based on the nucleotide sequence of the VP-1/VP-2 gene revealed that the Indian isolate closely resembled a CPV-2b Italian strain, showing 98.4% nucleotide sequence homology indicating very little genetic divergence since it was first identified in 1978.

Download Full-text

EVALUASI KIT DETEKSI CEPAT TERHADAP SAMPEL OTAK ANJING TERINFEKSI VIRUS RABIES

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences ◽

10.21157/j.ked.hewan.v9i1.2797 ◽

2015 ◽

Vol 9 (1) ◽

Author(s):

Michael Haryadi Wibowo ◽

Tri Untari ◽

Sidna Artanto ◽

Surya Amanu ◽

AETH. Wahyuni ◽

...

Keyword(s):

Escherichia Coli ◽

Polymerase Chain Reaction ◽

Fluorescent Antibody ◽

Rapid Test ◽

Canine Parvovirus ◽

Fluorescent Antibody Technique ◽

Chain Reaction ◽

Antibody Technique ◽

Test Kit ◽

Polymerase Chain

Penelitian ini bertujuan mengetahui potensi kit deteksi cepat Anigen® rapid test kit rabies Ag dalam mendeteksi virus rabies pada sampel otakanjing yang diperoleh dari lapangan yang meliputi batas deteksi, kecepatan reaksi, uji reaksi silang, uji sensitivitas, dan spesifisitas. Batas deteksi ditentukan dengan pengenceran secara serial kontrol positif virus rabies dan selanjutnya diuji dengan rapid test kit sesuai petunjuk produsen. Uji reaksi silang dilakukan dengan canine parvovirus, Escherichia coli, dan Salmonella sp. Uji sensitivitas dan spesifisitas dilakukan terhadap sampel otak yang telah dikonfirmasi positif rabies dengan uji fluorescent antibody technique. Konfirmasi uji rapid test tersebut dilakukan dengan reverse transcriptase polymerase chain reaction. Berdasarkan data yang diperoleh dalam penelitian ini dapat disimpulkan bahwa Anigen® rapid test kit rabies Ag mampu mendeteksi sampel yang mengandung virus rabies dengan titer 0,5 x log 106,5/0,03 ml, dengan rata-rata kecepatan reaksi 1,8 menit 29,35 detik (kurang dari 2 menit). Di samping itu Anigen® rapid test kit rabies menunjukkan tidak terdapat reaksi silang dengan canine parvovirus, Escherichia coli, dan Salmonella sp. serta mempunyai sensitivitas 92,30% dan spesifisitas 97,90%

Download Full-text

Phylogenetic analysis of canine parvovirus partial VP2 gene in India

Virus Genes ◽

10.1007/s11262-013-1000-5 ◽

2013 ◽

Vol 48 (1) ◽

pp. 89-95 ◽

Cited By ~ 19

Author(s):

H. K. Mukhopadhyay ◽

Samyukta Lakshmi Matta ◽

S. Amsaveni ◽

P. X. Antony ◽

J. Thanislass ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Canine Parvovirus ◽

Vp2 Gene

Download Full-text

Dynamic evolution of canine parvovirus in Thailand

Veterinary World ◽

10.14202/vetworld.2020.245-255 ◽

2020 ◽

Vol 13 (2) ◽

pp. 245-255

Author(s):

N. Inthong ◽

S. Kaewmongkol ◽

N. Meekhanon ◽

K. Sirinarumitr ◽

T. Sirinarumitr

Keyword(s):

Amino Acids ◽

Clinical Signs ◽

Canine Parvovirus ◽

Specific Primers ◽

Highly Pathogenic ◽

Vp2 Gene ◽

Fecal Samples ◽

Feline Panleukopenia Virus ◽

Close Relationship ◽

Polymerase Chain

Background and Aim: According to the previous study, the circulating canine parvovirus (CPV) in Thailand is 2a and 2b. Nowadays, CPV mutants, including CPV-2c, have been identified in many parts of the world. This study aimed to investigate the genetic diversity of the circulating CPV in Thailand. Materials and Methods: Eighty-five CPV-positive fecal samples were obtained from dogs with either acute hemorrhagic diarrhea or diarrhea. The complete VP2 gene of these samples was amplified using VP2 specific primers and polymerase chain reaction (PCR). The obtained full-length VP2 sequences were analyzed and a phylogenetic tree was constructed. Results: Sixty and 25 CPV-positive fecal samples were collected in 2010 and 2018, respectively. Thirty-four samples were new CPV-2a and 31 samples were new CPV-2b due to amino acids substitution at position 297 (Ser-Ala). In 2018, 5 new CPV-2a, 19 CPV-2c, and 1 feline panleukopenia virus (FPV) were found, but no new CPV-2b was detected. Moreover, most of the CPV in this study had amino acids mutations at positions 324 and 440. The phylogenetic construction demonstrated the close relationship between the current new CPV-2a with the previous CPV-2a reported from Thailand, China, Uruguay, Vietnam, Singapore, and India. Interestingly, the current new CPV-2b in this study was not closely related to the previous CPV-2b reported in Thailand. The CPV-2c in this study was closer to Asian CPV-2c and further from either European or South America CPV-2c. Interestingly, FPV was identified in a diarrhea dog. Conclusion: The evolution of CPV in Thailand is very dynamic. Thus, it is important to monitor for CPV mutants and especially the clinical signs relating to these mutants to conduct surveillance for the emergence of new highly pathogenic CPV in the future.

Download Full-text

Should IgM/IgG rapid test kit be used in the diagnosis of COVID-19?

Acta Medica Philippina ◽

10.47895/amp.v54i0.1558 ◽

2020 ◽

Vol 54 ◽

Author(s):

Aldrich Ivan Lois D. Burog ◽

Clarence Pio Rey C. Yacapin ◽

Renee Rose O. Maglente ◽

Anna Angelica Macalalad-Josue ◽

Elenore Judy B. Uy ◽

...

Keyword(s):

Direct Detection ◽

Rapid Test ◽

Definitive Diagnosis ◽

Rapid Review ◽

Current Evidence ◽

Rt Pcr ◽

Qualitative Detection ◽

Test Kit ◽

Polymerase Chain ◽

Antibody Tests

KEY FINDINGS Current evidence does NOT support use of IgM/IgG rapid test kits for the definitive diagnosis of COVID-19 in currently symptomatic patients. • The present standard for diagnosis of COVID-19 is through qualitative detection of COVID-19 virus nucleic acid via reverse transcription polymerase chain reaction (RT-PCR). • Due to long turnaround times and complicated logistical operations, a rapid and simple field test alternative is needed to diagnose and screen patients. • An alternative to the direct detection and measurement of viral load (RT-PCR) is the qualitative detection of specific antibodies to COVID-19. ELISA (discussed in a separate rapid review) and lateral flow immunoassay (LFIA) IgM/IgG rapid test kits are two currently available, qualitative, antibody tests for COVID-19. • Two low quality clinical trials showed that there is insufficient evidence to support the use of IgM/IgG rapid test kits for the definitive diagnosis of COVID-19. Diagnostic accuracy varies greatly depending on the timing of the test. The test performed very poorly during the early phase of the disease (i.e., less than eight days from onset of symptoms). • Existing guidelines do not recommend serologic antibody tests for the diagnosis of COVID-19 in currently symptomatic patients.

Download Full-text

Phylogenetic Analysis of Canine Parvovirus VP2 Gene in Taiwan

Virus Genes ◽

10.1007/s11262-005-1791-0 ◽

2005 ◽

Vol 31 (2) ◽

pp. 171-174 ◽

Cited By ~ 35

Author(s):

Hsien-Chi Wang ◽

Wei-Da Chen ◽

Shiun-Long Lin ◽

Jacky Peng-Wen Chan ◽

Min-Liang Wong

Keyword(s):

Phylogenetic Analysis ◽

Canine Parvovirus ◽

Vp2 Gene

Download Full-text

Phylogenetic analysis of VP2 gene of canine parvovirus and comparison with Indian and world isolates

Acta Virologica ◽

10.4149/av_2016_01_106 ◽

2016 ◽

Vol 60 (01) ◽

pp. 106-110 ◽

Cited By ~ 1

Author(s):

G. KAUR ◽

M. CHANDRA ◽

P. N. DWIVEDI

Keyword(s):

Phylogenetic Analysis ◽

Canine Parvovirus ◽

Vp2 Gene

Download Full-text

Molecular typing of canine parvovirus from Sulaimani, Iraq and phylogenetic analysis using partial VP2 gene

BULGARIAN JOURNAL OF VETERINARY MEDICINE ◽

10.15547/bjvm.1032 ◽

2017 ◽

Vol 20 (3) ◽

pp. 225-235 ◽

Cited By ~ 1

Author(s):

M. Baba Sheikh ◽

P. Rashid ◽

A. Marouf ◽

Z. Raheem ◽

S. Manjunath ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Molecular Typing ◽

Canine Parvovirus ◽

Vp2 Gene

Download Full-text

Efektifitas penggunaan filgastrim untuk terapi leukopenia pada kasus infeksi canine parvovirus

ARSHI Veterinary Letters ◽

10.29244/avl.4.3.51-52 ◽

2021 ◽

Vol 4 (3) ◽

pp. 51-52

Author(s):

Tiara Putri Sajuthi ◽

Anisa Hasby Fauzia ◽

Christophorus Algriawan Bayu Widjanarko

Keyword(s):

Rapid Test ◽

Canine Parvovirus ◽

Granulocyte Colony Stimulating Factor ◽

Colony Stimulating Factor ◽

Cocker Spaniel ◽

Test Kit ◽

Mixed Breed ◽

Stimulating Factor

Canine parvovirus (CPV) adalah salah satu penyakit infeksius pada anjing dengan angka morbiditas dan mortalitas yang tinggi. Infeksi CPV umumnya menyerang organ sistem pencernaan sehingga mengakibatkan gejala klinis berupa muntah dan diare. Organ lain yang diserang oleh virus ini antara lain sumsum tulang, sistem limfatik, dan myocardium (terutama pada hewan muda). Salah satu gejala klinis yang kerap muncul adalah leukopenia akibat perusakan prekursor leukosit dan sel-sel limfoid oleh virus. Filgastrim (Leucogen®) adalah rekombinan Human Granulocyte – Colony Stimulating Factor (rH G-CSF) yang berfungsi menstimulasi proliferasi dan diferensiasi sel-sel granulosit. Tiga ekor anjing ras Cocker Spaniel (a) (2 tahun), Bernese Mountain (b) (6 bulan), dan mixed breed (c) (3 bulan) dibawa ke PDHB 24 Jam drh. Cucu K. Sajuthi, dkk. dengan kondisi klinis anorexia, lethargy, vomit, melena, dan dehidrasi. Uji rapid test kit CPV menunjukan interpretasi positif. Pemeriksaan darah lengkap menunjukan nilai WBC 2 x 103/µL (a); 3,5 x 103/µL (b); dan 3,6 x 103/µL (c), serta nilai absolut granulosit 1,1 x 103/µL (a); 2,1 x 103/µL (b); dan 2,6 x 103/µL (c). Ketiga pasien diberikan terapi filgastrim (10 µg/kg/hari) selama tiga hari berturut-turut bersama dengan terapi suportif lainnya. Darah lengkap kembali dilakukan pemeriksaan dan menunjukan nilai WBC 5,8 x 103/µL (a); 9,7 x 103/µL (b); dan 51,4 x 103/µL (c) serta nilai absolut granulosit 3,4 x 103/µL (a); 7,3 x 103/µL (b); dan 42,5 x 103/µL (c). Terapi menggunakan filgastrim pada ketiga pasien menunjukkan peningkatan kondisi yang nyata terhadap gejala leukopenia. Pasien juga menunjukan perbaikan kondisi klinis yang signifikan membaik.

Download Full-text