Phylogenetic analysis of VP2 gene of canine parvovirus and comparison with Indian and world isolates

Aim: This study aimed to detect and characterize current genotypes of canine parvovirus (CPV) in Egypt during 2018. Materials and Methods: A total of 50 fecal swabs were collected from clinically infected domestic dogs of 2-5 months of age, suspected to suffer from CPV infection, from Cairo and Giza Governorates. The samples were subjected to qualitative antigen detection using the rapid test, followed by isolation on Madin-Darby Canine Kidney (MDCK) cells, molecular characterization with partial amplification of VP2 gene using polymerase chain reaction (PCR), followed by sequencing and phylogenetic analysis. Results: Out of 50 fecal samples, 20 samples were positive (40%) by Rapid CPV/canine coronavirus Ag Test Kit. These positive samples were cultured successfully on MDCK cells. Nine randomly chosen samples out of 30 apparently negative samples were amplified using PCR with primers Hfor and Hrev to yield a typical 630 bp fragment. Then, six randomly chosen samples out of nine were amplified using PCR with primers Pbs and Pbas to yield a typical 427 bp fragment. Sequencing, BLAST analysis and assembly of the two fragments (630 bp and 427 bp) to produce 912 bp fragments, in the six samples, revealed two serotypes CPV-2b and CPV-2c. The obtained strains were submitted to GenBank and given accession numbers MK642272, MK642273, MK642274, MK642275, MK642276, and MK642277. Phylogenetic analysis of the Egyptian strains serotype 2b illustrated that they were closely related to Thailand strains (accession numbers KP715709, KP715694, KP715701, and KP715700); while Egyptian strains serotype 2c was closely related to Thailand strains (accession numbers MH711894 and MH711902), Taiwanese strain (KU244254), Chinese strain (MF467242), and Vietnamese strain (accession number LC216910). Conclusion: The current research recommends further epidemiological studies to assess the extent of the occurrence of different serotypes of CPV in Egypt and the efficiency of imported and locally produced vaccines in protection against CPV infection.

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Phylogenetic Analysis of Canine Parvovirus VP2 Gene in China

Transboundary and Emerging Diseases ◽

10.1111/tbed.12268 ◽

2014 ◽

Vol 63 (2) ◽

pp. e262-e269 ◽

Cited By ~ 21

Author(s):

L. Yi ◽

M. Tong ◽

Y. Cheng ◽

W. Song ◽

S. Cheng

Keyword(s):

Phylogenetic Analysis ◽

Canine Parvovirus ◽

Vp2 Gene

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Prevalence and molecular characterization of canine parvovirus

Veterinary World ◽

10.14202/vetworld.2021.603-606 ◽

2021 ◽

Vol 14 (3) ◽

pp. 603-606

Author(s):

Parikshit Singh ◽

Gurpreet Kaur ◽

Mudit Chandra ◽

P. N. Dwivedi

Keyword(s):

Phylogenetic Analysis ◽

Sequence Analysis ◽

Clinical Signs ◽

Canine Parvovirus ◽

Vp2 Gene ◽

Northern India ◽

Percent Positivity ◽

Northern Regions ◽

Vaccination History ◽

Antigenic Type

Background and Aim: Canine parvovirus (CPV) belonging to family Parvoviridae causes hemorrhagic gastroenteritis in dogs and heavy mortality in young dogs. The virus has three structural (VP1, VP2 and VP3) and two non-structural proteins (NS1 and NS2), VP2 being highly immunogenic. This study aims to study molecular epidemiology of CPV by sequence analysis of VP2 gene to determine the prevailing antigenic type(s) in the northern regions of India. Materials and Methods: A total of 118 rectal swabs collected from dogs exhibiting clinical signs of CPV infection were processed for the isolation of DNA and subjected to polymerase chain reaction (PCR) and nested PCR (NPCR). A total of 13 NPCR products selected randomly were subjected to sequence analysis of VP2 gene. Results: The percent positivity of CPV was found 28% and 70% by PCR and NPCR, respectively. Dogs with vaccination history against CPV too were found positive with a percent positivity of 24.10%. Gene sequencing and phylogenetic analysis of VP2 gene from these isolates revealed that most samples formed a clade with CPV-2a isolates. Conclusion: Sequence analysis and phylogenetic analysis of VP2 gene in the studied regions of northern India revealed that CPV-2a was the most prevalent antigenic type.

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Detection of CPV 2a Antigen Type of Canine Parvovirus in the States of Punjab and Assam, India

Current Journal of Applied Science and Technology ◽

10.9734/cjast/2019/v38i230354 ◽

2019 ◽

pp. 1-7

Author(s):

Himasri Das ◽

Gurpreet Kaur ◽

Mudit Chandra ◽

P. N. Dwivedi

Keyword(s):

Phylogenetic Analysis ◽

Sequence Analysis ◽

Nested Pcr ◽

Canine Parvovirus ◽

Vp2 Gene ◽

North Eastern ◽

North Part ◽

Emerging Virus ◽

Antigenic Type ◽

North Eastern Part

Aim: Canine Parvovirus (CPV) is an emerging and re-emerging virus of canines. The study was undertaken to analyze VP2 gene of CPV in the isolates from dogs positive for CPV infection. Methods: The rectal swabs were collected from dogs suspected of CPV and subjected to PCR and nested PCR. The regions compared in the study were Punjab to represent north part of India and Assam to represent north-eastern part of India. The sequence analysis of VP2 gene of CPV was done using NCBI BLAST from the isolates which were positive for CPV by nested PCR. Further, phylogenetic analysis was done to understand the prevailing antigenic type of CPV from northern and north eastern part of India. Results: The sequence analysis revealed that all the sequences of VP2 gene from the samples had 98-99% homology with Canine Parvovirus and phylogenetic analysis revealed that CPV 2a antigenic type is circulating in both the regions selected in the study. Conclusions: The present study revealed that CPV 2a is circulating in the regions of Punjab and Assam part of India. Further sequence analysis of VP2 gene from more number of field isolates can throw better light on the prevailing antigenic type of CPV in various parts of India.

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Molecular characterization and phylogenetic analysis of a canine parvovirus isolate in India

Veterinární Medicína ◽

10.17221/147/2009-vetmed ◽

2009 ◽

Vol 54 (No. 10) ◽

pp. 483-490 ◽

Cited By ~ 5

Author(s):

S. Nandi ◽

S. Chidri ◽

M. Kumar

Keyword(s):

Phylogenetic Analysis ◽

Nucleotide Sequence ◽

Culture System ◽

Mdck Cells ◽

Canine Parvovirus ◽

Cell Culture System ◽

Chain Reaction ◽

Vp2 Gene ◽

Polymerase Chain ◽

Hemorrhagic Enteritis

Canine parvovirus 2 (CPV-2) is the causative agent of acute hemorrhagic enteritis and myocarditis in dogs. In this study the nucleotide sequence of the VP1/VP2 gene of a CPV isolate from India was analyzed and the phylogenetic relationship with other CPV isolates was established. Out of 36 samples analyzed, 16 were found positive for CPV-2 by polymerase chain reaction (PCR). Among the 16 positive samples, five were inoculated in MDCK cells for isolation out of which one adapted successfully to the cell culture system. Phylogenetic analysis based on the nucleotide sequence of the VP-1/VP-2 gene revealed that the Indian isolate closely resembled a CPV-2b Italian strain, showing 98.4% nucleotide sequence homology indicating very little genetic divergence since it was first identified in 1978.

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Cerebellar hypoplasia and dysplasia in a juvenile raccoon with parvoviral infection

Journal of Veterinary Diagnostic Investigation ◽

10.1177/1040638720912229 ◽

2020 ◽

Vol 32 (3) ◽

pp. 463-466 ◽

Cited By ~ 1

Author(s):

Arno Wünschmann ◽

Robert Lopez-Astacio ◽

Anibal G. Armien ◽

Colin R. Parrish

Keyword(s):

Granule Cells ◽

Nonstructural Protein ◽

Canine Parvovirus ◽

Cerebellar Hypoplasia ◽

Diagnostic Laboratory ◽

Vp2 Gene ◽

Nonstructural Protein 1 ◽

Cell Processes ◽

Ns1 Gene ◽

Pcr Targeting

A juvenile raccoon ( Procyon lotor) was submitted dead to the Minnesota Veterinary Diagnostic Laboratory for rabies testing without history. The animal had marked hypoplasia of the cerebellum. Histology demonstrated that most folia lacked granule cells and had randomly misplaced Purkinje cells. Immunohistochemistry revealed the presence of parvoviral antigen in a few neurons and cell processes. PCR targeting feline and canine parvovirus yielded a positive signal. Sequencing analyses from a fragment of the nonstructural protein 1 ( NS1) gene and a portion of the viral capsid protein 2 ( VP2) gene confirmed the presence of DNA of a recent canine parvovirus variant (CPV-2a–like virus) in the cerebellum. Our study provides evidence that (canine) parvovirus may be associated with cerebellar hypoplasia and dysplasia in raccoons, similar to the disease that occurs naturally and has been reproduced experimentally by feline parvoviral infection of pregnant cats, with subsequent intrauterine or neonatal infections of the offspring.

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A phylogenetic study of canine parvovirus type 2c in midwestern Brazil

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2013000200013 ◽

2013 ◽

Vol 33 (2) ◽

pp. 214-218 ◽

Cited By ~ 10

Author(s):

Danúbia S. Fontana ◽

Paulo Ricardo D. Rocha ◽

Raquel A.S. Cruz ◽

Letícya L. Lopes ◽

Andréia L.T. Melo ◽

...

Keyword(s):

Amino Acid ◽

Genetic Variability ◽

Dna Sequencing ◽

Canine Parvovirus ◽

Phylogenetic Study ◽

Vp2 Gene ◽

Hemorrhagic Enteritis ◽

Canine Parvovirus Type 2 ◽

Representative Samples

Since the late 1970s, canine parvovirus type 2 (CPV-2) has emerged as a causative agent of fatal severe acute hemorrhagic enteritis in dogs. To date, three antigenic types of CPV-2 were described worldwide (CPV-2a/b/c). This study was conducted to determine the variants of CPV-2 circulating in dogs from the Cuiabá Municipality in Midwestern Brazil. Out of 50 fecal samples, collected between 2009 and 2011, 27 tested positive for CPV-2. A 583 bp fragment of the VP2 gene was amplified by PCR, 13 representative samples were analyzed further by DNA sequencing. All strains were characterized as CPV-2c, displayed a low genetic variability although observed several amino acid substitution. These findings indicated that CPV-2c has been circulating in dogs from the Cuiabá Municipality in Midwestern Brazil.

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