scholarly journals Production of Microbial Exopolysaccharide by Cost-effective Medium Opimization Method

2019 ◽  
pp. 1-13
Author(s):  
I. G. Nwosu ◽  
G. O. Abu ◽  
K. O. Agwa
Keyword(s):  
Sweet Potato ◽  
Fermentation Broth ◽  
Maximum Yield ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Agricultural Wastes ◽  
Efficient Production ◽  
Total Carbohydrate ◽  
Carbon And Nitrogen ◽  
Extract Medium

Microbial exopolysaccharide (EPS) emerged as a fast and high yielding sustainable polymeric substance which can be used as an alternative to synthetic polymer in industry. In this study, the influence of various nutritional and environmental factors of fermentation medium on bacterial growth and EPS production was evaluated by one factor at a time optimization. Efficient production medium was chosen from four different basal media and its carbon and nitrogen substrates were varied among organic and inorganic sources. Feasibility of bacterial utilization of some agricultural wastes as carbon and nitrogen sources to synthesize exopolysaccharide was compared. Carbon source of the fermentation medium was replaced with hydrolysates of sugarcane baggasse (SCB), sweet potato peels (SPP) or ripe plantain peels (RPP) at various concentrations while the nitrogen substrates was replaced with extracts of poultry droppings (PP), groundnut pod (GP) or beans bran (BB). Response results observed from single factor optimization were explored as center points to design a model for Response Surface Methodology study. Cell growth was determined from the biomass population of the fermentation broth after 5 days of incubation in a rotary shaker at 120 rpm at 30ºC. EPS was precipitated with pre-chilled ethanol (at 4ºC) from cell-free broth and overnight incubation at 4ºC. Total carbohydrate content was estimated by phenol-sulphuric acid method. Result obtained showed that 2% concentration Hydrolysate of SPP containing medium gave maximum yield of 2.26 g EPS/l of the medium as compared to sucrose containing medium with yield of 1.25 g EPS/l of the medium while highest production yield of 9.46 gEPS/l of the medium was obtained from 10 g/l BB extract medium as compared to yeast extract medium (5.41 gEPS/l). Results indicated that agricultural wastes such as sweet potato peel hydrolysate and bean bran extract could be developed as inexpensive alternative route to synthesize EPS from bacteria than inorganic substrates.

Production of Microbial Exopolysaccharide by Cost-effective Medium Optimization Method

2019 ◽  
pp. 1-12
Author(s):  
I. G. Nwosu ◽  
G. O. Abu ◽  
K. O. Agwa
Keyword(s):  
Sweet Potato ◽  
Fermentation Broth ◽  
Maximum Yield ◽  
Optimization Method ◽  
Fermentation Medium ◽  
Agricultural Wastes ◽  
Efficient Production ◽  
Total Carbohydrate ◽  
Carbon And Nitrogen ◽  
Extract Medium

Microbial exopolysaccharide (EPS) emerged as a fast and high yielding sustainable polymeric substance which can be used as an alternative to synthetic polymer in industry. In this study, the influence of various nutritional and environmental factors of fermentation medium on bacterial growth and EPS production was evaluated by one factor at a time optimization. Efficient production medium was chosen from four different basal media and its carbon and nitrogen substrates were varied among organic and inorganic sources. Feasibility of bacterial utilization of some agricultural wastes as carbon and nitrogen sources to synthesize exopolysaccharide was compared. Carbon source of the fermentation medium was replaced with hydrolysates of sugarcane baggasse (SCB), sweet potato peels (SPP) or ripe plantain peels (RPP) at various concentrations while the nitrogen substrates was replaced with extracts of poultry droppings (PP), groundnut pod (GP) or beans bran (BB). Response results observed from single factor optimization were explored as center points to design a model for Response Surface Methodology study. Cell growth was determined from the biomass population of the fermentation broth after 5days of incubation in a rotary shaker at 120 rpm at 30oC. EPS was precipitated with pre-chilled ethanol (at 4oC) from cell-free broth and overnight incubation at 4oC. Total carbohydrate content was estimated by phenol-sulphuric acid method. Result obtained showed that 2% concentration Hydrolysate of SPP containing medium gave maximum yield of 2.26g EPS/l of the medium as compared to sucrose containing medium with yield of 1.25 g EPS/l of the medium while highest production yield of 9.46 gEPS/l of the medium was obtained from 10 g/l BB extract medium as compared to yeast extract medium (5.41 gEPS/l). Results indicated that agricultural wastes such as sweet potato peel hydrolysate and bean bran extract could be developed as inexpensive alternative route to synthesize EPS from bacteria than inorganic substrates.

scholarly journals Optimization of Cultural Conditions for Production of Antibacterial Metabolites from Streptomyces coelicoflavus BC 01

2015 ◽  
Vol 7 (2) ◽  
pp. 151-159
Author(s):  
Kothagorla Venkata RAGHAVA RAO ◽  
Dadi BHASKARA RAO ◽  
Botcha SATYANARAYANA ◽  
Tamanam RAGHAVA RAO
Keyword(s):  
Andhra Pradesh ◽  
Maximum Yield ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Carbon And Nitrogen ◽  
Mangrove Soil ◽  
Cultural Conditions ◽  
Bean Meal ◽  
Metabolites Production ◽  
Soya Bean Meal

The aim of the present study was to optimize various cultural conditions for the production of antibacterial metabolites by Streptomyces coelicoflavus BC 01 isolated from mangrove soil, Visakhapatnam, Andhra Pradesh, India. The effect of various factors such as carbon and nitrogen sources, different concentrations of NaCl and K2HPO4, different temperature, pH, incubation time and agitation on antibacterial metabolites production were studied. The production of antibacterial metabolites by the isolate Streptomyces coelicoflavus BC 01 was greatly influenced by the cultural conditions. Glucose (1.2%) and soya bean meal (1%) seemed to be the best carbon and nitrogen source respectively, followed by NaCl (1%) and K2HPO4 (0.25%). Maximum production of antibacterial metabolites was observed at a temperature of 30 °C, with pH 7.2, at 160 rpm for 96 hrs. These optimized parameters can be further useful to design a fermentation medium to achieve maximum yield of antibacterial metabolites from Streptomyces coelicoflavus BC 01.

scholarly journals Screening of Penicillium Species and Optimisation of Culture Conditions for the Production of Ergot Alkaloids Using Surface Culture Fermentation Process

2015 ◽  
Vol 58 (1) ◽  
pp. 23-29
Author(s):  
Memuna Ghafoor Shahid ◽  
Muhammad Nadeem
Keyword(s):  
Maximum Yield ◽  
Ergot Alkaloids ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Culture Conditions ◽  
Fungal Species ◽  
Surface Culture ◽  
Penicillium Species ◽  
Carbon And Nitrogen ◽  
Culture Process

The present study deals with the screening of fungal species and suitable fermentation medium for the production of ergot alkaloids. Various species of genus Penicillium were grown on differentfermentation media by employing surface culture fermentation technique to achieve the most suitable medium and the best Penicillium sp. The results showed that medium M5 gave maximum yield withPenicillium commune. Different culture conditions such as effect of different carbon and nitrogen sources, their concentration levels, different pH values and sizes of inoculum on the production of ergot alkaloids were also studied to improve the yield. Maximum production of ergot alkaloids (4.32 mg/L) was achieved with 15 mL spore suspension at pH 5 in fermentation medium containing 35% (w/v) sucrose. All these  results indicate that culture conditions are very much crucial to improve the yield of ergot alkaloids produced by Penicillium commune through surface culture process. 

scholarly journals A highly thermotolerant laccase produced by Cerrena unicolor strain CGMCC 5.1011 for complete and stable malachite green decolorization

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yanhua Yao ◽  
Guimei Zhou ◽  
Yonghui Lin ◽  
Xinqi Xu ◽  
Jie Yang
Keyword(s):  
Malachite Green ◽  
Fermentation Broth ◽  
Nitrogen Sources ◽  
Life Time ◽  
Industrial Applications ◽  
Dynamics Simulation ◽  
Carbon And Nitrogen ◽  
Cdna Sequences ◽  
Cerrena Unicolor ◽  
Future Work

Abstract Laccases are a class of multi-copper oxidases with important industrial values. A thermotolerant laccase produced by a basidiomycete fungal strain Cerrena unicolor CGMCC 5.1011 was studied. With glycerin and peptone as the carbon and nitrogen sources, respectively, a maximal laccase activity of 121.7 U/mL was attained after cultivation in the shaking flask for 15 days. Transcriptomics analysis revealed an expressed laccase gene family of 12 members in C. unicolor strain CGMCC 5.1011, and the gene and cDNA sequences were cloned. A glycosylated laccase was purified from the fermentation broth of Cerrena unicolor CGMCC 5.1011 and corresponded to Lac2 based on MALDI-TOF MS/MS identification. Lac2 was stable at pH 5.0 and above, and was resistant to organic solvents. Lac2 displayed remarkable thermostability, with half-life time of 1.67 h at 70 ºC. Consistently, Lac2 was able to completely decolorize malachite green (MG) at high temperatures, whereas Lac7 from Cerrena sp. HYB07 resulted in accumulation of colored MG transformation intermediates. Molecular dynamics simulation of Lac2 was conducted, and possible mechanisms underlying Lac2 thermostability were discussed. The robustness of C. unicolor CGMCC 5.1011 laccase would not only be useful for industrial applications, but also provide a template for future work to develop thermostable laccases.

Hydrolysis of Corncob Residue by Enzymes from Fungi C1067 and the Products Analysis by Capillary Electrophoresis

2011 ◽  
Vol 145 ◽  
pp. 68-72
Author(s):  
Jian Lu Ma ◽  
Xiao Min Fang ◽  
Xin Jiong Guo ◽  
Huan Fei Liu ◽  
Gui Fu Dai ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Corn Steep Liquor ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Citrate Buffer ◽  
Carbon And Nitrogen ◽  
Products Analysis ◽  
Steep Liquor ◽  
Hydrolysis Of ◽  
Assay Conditions

To achieve high enzymatic hydrolytic efficiency of corncob residue, one strain, Trichoderma C1067, with the enzyme activity of corncob residue as high as 4.5g/L glucose liberated per hour in 0.5mL crude cellulase and 0.5mL citric acid /sodium citrate buffer under the assay conditions, was carefully studied. The optimal carbon and nitrogen sources used in the fermentation medium are as follows: corncob residues 2.0%, wheat bran 1.5%, corncob 0.5%, rice straw 0.5%, peptone 0.1% and corn steep liquor 4%. The optimal conditions of saccharification for corncob are 55°C and pH 4.0. The sugars gained in the hydrolysate consist of glucose 4.1% and xylose 0.4% after enzymolysis for 72h, tested by capillary electrophoresis.

scholarly journals Lab-Scale Optimization of Polyhydroxyalkanoate Production by Bacterial Strain cmg1415 on Local Cheap Substrates Using One Variable at a Time Approach

2021 ◽  
Vol 1 (1) ◽  
pp. 25-31
Author(s):  
Muhammadi Muhammadi ◽  
Shabina Shafiq
Keyword(s):  
Sugar Beet ◽  
Minimal Medium ◽  
Maximum Yield ◽  
Nitrogen Sources ◽  
Sole Source ◽  
Labor Cost ◽  
Culture Conditions ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Scale Optimization

Production of polyhydroxyalkanoate (PHA) under optimum culture conditions using local cheap feedstocks is indispensable to overcome the current cost of PHA-based plastics. For this purpose, optimum culture conditions and cheap feedstocks were investigated to produce maximum yield of PHA in CMG1415. Maximum yield was obtained with sucrose or sugar beet as sole source of precursors for PHA in 8 days of incubation at 35 °C in a minimal medium adjusted at pH 7. Further, for maximum yield no mechanical shaking was needed. Local cheap feedstock such as sugar beet and molasses were found to play as significant carbon and nitrogen sources for maximum PHA yield.  Bacterial plastic produced under these low-labor-cost culture conditions may to reduce the present cost of degradable bioplastic and be much effective alternate of nondegradable varieties of synthetic plastic.

scholarly journals A Highly Thermotolerant Laccase Produced by Cerrena Unicolor Strain CGMCC 5.1011 for Complete and Stable Malachite Green Decolorization

2020 ◽  
Author(s):  
Yanhua Yao ◽  
Guimei Zhou ◽  
Yonghui Lin ◽  
Xinqi Xu ◽  
Jie Yang
Keyword(s):  
Malachite Green ◽  
Fermentation Broth ◽  
Nitrogen Sources ◽  
Life Time ◽  
Industrial Applications ◽  
Dynamics Simulation ◽  
Carbon And Nitrogen ◽  
Cdna Sequences ◽  
Cerrena Unicolor ◽  
Future Work

Abstract Laccases are a class of multi-copper oxidases with important industrial values. A thermotolerant laccase produced by a basidiomycete fungal strain Cerrena unicolor CGMCC 5.1011 was studied. With glycerin and peptone as the carbon and nitrogen sources, respectively, a maximal laccase activity of 121.7 U/mL was attained after cultivation in the shaking flask for 15 days. Transcriptomics analysis revealed an expressed laccase gene family of 12 members in C. unicolor strain CGMCC 5.1011, and the gene and cDNA sequences were cloned. A glycosylated laccase was purified from the fermentation broth of Cerrena unicolor CGMCC 5.1011 and corresponded to Lac2 based on MALDI-TOF MS/MS identification. Lac2 was stable at pH 5.0 and above, and was resistant to organic solvents. Lac2 displayed remarkable thermostability, with half-life time of 1.67 h at 70 ºC. Consistently, Lac2 was able to completely decolorize malachite green (MG) at high temperatures, whereas Lac7 from Cerrena sp. HYB07 resulted in accumulation of colored MG transformation intermediates. Molecular dynamics simulation of Lac2 was conducted, and possible mechanisms underlying Lac2 thermostability were discussed. The robustness of C. unicolor CGMCC 5.1011 laccase would not only be useful for industrial applications, but also provide a template for future work to develop thermostable laccases.

scholarly journals Enhanced production of β- glucosidase by locally isolated fungal strain employing submerged fermentation

2019 ◽  
Vol 35 (5) ◽  
Author(s):  
Roheena Abdullah ◽  
Sobia Nazir Chudhary ◽  
Afshan Kaleem ◽  
Mehwish Iqtedar ◽  
Kinza Nisar ◽  
...  
Keyword(s):  
Submerged Fermentation ◽  
Wide Spectrum ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Fungal Strain ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Enhanced Production ◽  
Different Sources ◽  
Fermentation Media

β-glucosidase has wide spectrum of biotechnological applications in different industries including food, textile, laundry detergents, pulp and paper, pharmaceutical and biofuel industry. The present investigation related to isolation, screening, and process optimization of fungal strain for enhanced production of β-glucosidase (BGL). For this purpose, different fungal stains were isolated from different sources including soil, fruits, bark of tree as well as from the compost. The screening of fungal strain for BGL production was carried out via submerged fermentation. All the tested strains were identified on the basis of micro and macroscopic features. The fungal strain having greater ability for BGL synthesis among tested ones was identified as Aspergillus niger and given the code SBT-15. The process parameter including fermentation media, temperature, pH, rate of fermentation, carbon and nitrogen sources, volume of media were optimized. Five different fermentation media were evaluated M3 medium gave maximum production. The optimal conditions for BGL production was 72 hours of incubation at 40°C, pH 6 and 50 ml fermentation medium. Glucose (1%) and ammonium sulphate (3%) were optimized as best carbon and nitrogen sources, respectively.

Production of a Bioflocculant by Penicillium Sp. HHE-P7 Using Sugarcane Molasses

2012 ◽  
Vol 518-523 ◽  
pp. 453-459
Author(s):  
Li Fan Liu
Keyword(s):  
Rotation Speed ◽  
Fermentation Broth ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Medium Components ◽  
Flocculating Activity ◽  
Initial Ph

Bioflocculant MBF7 was produced by a novel bioflocculant-producing microorganism HHE-P7. In order to reduce the bioflocculant producing cost, culture experiments were conducted. The effects of medium components including carbon and nitrogen sources as well as culture conditions such as pH of molasses diluents, cultivating temperature, inoculum size were investigated. The results showed when the molasses waste was diluted at COD concentration of 2000 mg/L, the optimal culture conditions for MBF7 production by HHE-P7 were inoculum size 1% (v/v), initial pH 5, cultivating temperature 25°C at the rotation speed 150 r/min. Under such conditions, MBF7 had a flocculating activity of 83% for 5 g/L kaolin clay suspension. About 3.19 g crude bioflocculant could be recovered from 1.0 L of molasses fermentation broth.

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