scholarly journals A Highly Thermotolerant Laccase Produced by Cerrena Unicolor Strain CGMCC 5.1011 for Complete and Stable Malachite Green Decolorization

2020 ◽  
Author(s):  
Yanhua Yao ◽  
Guimei Zhou ◽  
Yonghui Lin ◽  
Xinqi Xu ◽  
Jie Yang
Keyword(s):  
Malachite Green ◽  
Fermentation Broth ◽  
Nitrogen Sources ◽  
Life Time ◽  
Industrial Applications ◽  
Dynamics Simulation ◽  
Carbon And Nitrogen ◽  
Cdna Sequences ◽  
Cerrena Unicolor ◽  
Future Work

Abstract Laccases are a class of multi-copper oxidases with important industrial values. A thermotolerant laccase produced by a basidiomycete fungal strain Cerrena unicolor CGMCC 5.1011 was studied. With glycerin and peptone as the carbon and nitrogen sources, respectively, a maximal laccase activity of 121.7 U/mL was attained after cultivation in the shaking flask for 15 days. Transcriptomics analysis revealed an expressed laccase gene family of 12 members in C. unicolor strain CGMCC 5.1011, and the gene and cDNA sequences were cloned. A glycosylated laccase was purified from the fermentation broth of Cerrena unicolor CGMCC 5.1011 and corresponded to Lac2 based on MALDI-TOF MS/MS identification. Lac2 was stable at pH 5.0 and above, and was resistant to organic solvents. Lac2 displayed remarkable thermostability, with half-life time of 1.67 h at 70 ºC. Consistently, Lac2 was able to completely decolorize malachite green (MG) at high temperatures, whereas Lac7 from Cerrena sp. HYB07 resulted in accumulation of colored MG transformation intermediates. Molecular dynamics simulation of Lac2 was conducted, and possible mechanisms underlying Lac2 thermostability were discussed. The robustness of C. unicolor CGMCC 5.1011 laccase would not only be useful for industrial applications, but also provide a template for future work to develop thermostable laccases.

scholarly journals A highly thermotolerant laccase produced by Cerrena unicolor strain CGMCC 5.1011 for complete and stable malachite green decolorization

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yanhua Yao ◽  
Guimei Zhou ◽  
Yonghui Lin ◽  
Xinqi Xu ◽  
Jie Yang
Keyword(s):  
Malachite Green ◽  
Fermentation Broth ◽  
Nitrogen Sources ◽  
Life Time ◽  
Industrial Applications ◽  
Dynamics Simulation ◽  
Carbon And Nitrogen ◽  
Cdna Sequences ◽  
Cerrena Unicolor ◽  
Future Work

Abstract Laccases are a class of multi-copper oxidases with important industrial values. A thermotolerant laccase produced by a basidiomycete fungal strain Cerrena unicolor CGMCC 5.1011 was studied. With glycerin and peptone as the carbon and nitrogen sources, respectively, a maximal laccase activity of 121.7 U/mL was attained after cultivation in the shaking flask for 15 days. Transcriptomics analysis revealed an expressed laccase gene family of 12 members in C. unicolor strain CGMCC 5.1011, and the gene and cDNA sequences were cloned. A glycosylated laccase was purified from the fermentation broth of Cerrena unicolor CGMCC 5.1011 and corresponded to Lac2 based on MALDI-TOF MS/MS identification. Lac2 was stable at pH 5.0 and above, and was resistant to organic solvents. Lac2 displayed remarkable thermostability, with half-life time of 1.67 h at 70 ºC. Consistently, Lac2 was able to completely decolorize malachite green (MG) at high temperatures, whereas Lac7 from Cerrena sp. HYB07 resulted in accumulation of colored MG transformation intermediates. Molecular dynamics simulation of Lac2 was conducted, and possible mechanisms underlying Lac2 thermostability were discussed. The robustness of C. unicolor CGMCC 5.1011 laccase would not only be useful for industrial applications, but also provide a template for future work to develop thermostable laccases.

Facile Degradation of Polyvinyl alcohol (PVA) by a novel bacterial strain Enterobacter cloacae VVD-MBB8 isolated from Gulf of Mannar, Tamil Nadu

2020 ◽  
Author(s):  
Prakash Shyam Karuppiah ◽  
Rajkumar Prabhakaran ◽  
Ramya Venkatesan ◽  
Sivabalan Sivasamy ◽  
L.R.Monisha Miriam
Keyword(s):  
Polyvinyl Alcohol ◽  
Tamil Nadu ◽  
Culture Medium ◽  
Nitrogen Sources ◽  
Enterobacter Cloacae ◽  
Industrial Applications ◽  
Gulf Of Mannar ◽  
Rrna Gene ◽  
Carbon And Nitrogen ◽  
Distance Analysis

Polyvinyl alcohol (PVA) is a synthetic polymer, widely used for industrial applications, and its contamination related to the ecosystem concerns an increasingly serious problem. In the present study, we account a novel, potent PVA degrader, VVD-MBB8, isolated from the Gulf of Mannar. The VVD-MBB8 was identified as Enterobacter cloacae based on the molecular analysis using 16S rRNA gene sequence, biochemical and evolutionary distance analysis. This is the first report for the PVA degrader from the Enterobacter genus. The results showed 83% of the PVA present in the culture medium was degraded by Enterobacter cloacae VVD-MBB8 after 48 hrs of incubation with 30⁰C at the agitation of 145rpm. The results obtained from the FTIR showed notable differences in the PVA degradation stretches (3000 to 3300cm-1, 2500 to 3000cm-1) at 24 and 48 hrs compared with control (before degradation of PVA). The effects of carbon and nitrogen sources for PVA degradation were identified and the results revealed nitrogen sources have a significant increase in PVA degrading enzyme production and the enzyme activity was 83 U/ml.

Production of a Bioflocculant by Penicillium Sp. HHE-P7 Using Sugarcane Molasses

2012 ◽  
Vol 518-523 ◽  
pp. 453-459
Author(s):  
Li Fan Liu
Keyword(s):  
Rotation Speed ◽  
Fermentation Broth ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Medium Components ◽  
Flocculating Activity ◽  
Initial Ph

Bioflocculant MBF7 was produced by a novel bioflocculant-producing microorganism HHE-P7. In order to reduce the bioflocculant producing cost, culture experiments were conducted. The effects of medium components including carbon and nitrogen sources as well as culture conditions such as pH of molasses diluents, cultivating temperature, inoculum size were investigated. The results showed when the molasses waste was diluted at COD concentration of 2000 mg/L, the optimal culture conditions for MBF7 production by HHE-P7 were inoculum size 1% (v/v), initial pH 5, cultivating temperature 25°C at the rotation speed 150 r/min. Under such conditions, MBF7 had a flocculating activity of 83% for 5 g/L kaolin clay suspension. About 3.19 g crude bioflocculant could be recovered from 1.0 L of molasses fermentation broth.

scholarly journals Production of Microbial Exopolysaccharide by Cost-effective Medium Opimization Method

2019 ◽  
pp. 1-13
Author(s):  
I. G. Nwosu ◽  
G. O. Abu ◽  
K. O. Agwa
Keyword(s):  
Sweet Potato ◽  
Fermentation Broth ◽  
Maximum Yield ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Agricultural Wastes ◽  
Efficient Production ◽  
Total Carbohydrate ◽  
Carbon And Nitrogen ◽  
Extract Medium

Microbial exopolysaccharide (EPS) emerged as a fast and high yielding sustainable polymeric substance which can be used as an alternative to synthetic polymer in industry. In this study, the influence of various nutritional and environmental factors of fermentation medium on bacterial growth and EPS production was evaluated by one factor at a time optimization. Efficient production medium was chosen from four different basal media and its carbon and nitrogen substrates were varied among organic and inorganic sources. Feasibility of bacterial utilization of some agricultural wastes as carbon and nitrogen sources to synthesize exopolysaccharide was compared. Carbon source of the fermentation medium was replaced with hydrolysates of sugarcane baggasse (SCB), sweet potato peels (SPP) or ripe plantain peels (RPP) at various concentrations while the nitrogen substrates was replaced with extracts of poultry droppings (PP), groundnut pod (GP) or beans bran (BB). Response results observed from single factor optimization were explored as center points to design a model for Response Surface Methodology study. Cell growth was determined from the biomass population of the fermentation broth after 5 days of incubation in a rotary shaker at 120 rpm at 30ºC. EPS was precipitated with pre-chilled ethanol (at 4ºC) from cell-free broth and overnight incubation at 4ºC. Total carbohydrate content was estimated by phenol-sulphuric acid method. Result obtained showed that 2% concentration Hydrolysate of SPP containing medium gave maximum yield of 2.26 g EPS/l of the medium as compared to sucrose containing medium with yield of 1.25 g EPS/l of the medium while highest production yield of 9.46 gEPS/l of the medium was obtained from 10 g/l BB extract medium as compared to yeast extract medium (5.41 gEPS/l). Results indicated that agricultural wastes such as sweet potato peel hydrolysate and bean bran extract could be developed as inexpensive alternative route to synthesize EPS from bacteria than inorganic substrates.

scholarly journals Fibers Effects on Contract Turbulence Using a Coupling Euler Model

2021 ◽  
Vol 11 (15) ◽  
pp. 7126
Author(s):  
Wei Yang ◽  
Pei Hu
Keyword(s):  
Boundary Layer ◽  
Velocity Profile ◽  
Fiber Orientation ◽  
Bottom Layer ◽  
Orientation Distribution ◽  
Industrial Applications ◽  
Dynamics Simulation ◽  
Fiber Concentration ◽  
Fibers Orientation ◽  
Process Strategy

Fiber additive will induce the rheological behavior of suspension, resulting in variation in velocity profile and fiber orientation especially for the non-dilute case. Based on the fluid-solid coupling dynamics simulation, it shows that the fiber orientation aligns along the streamline more and more quickly in the central turbulent region as the fiber concentration increases, especially contract ratio Cx > 4. However, fibers tend to maintain the original uniform orientation and are rarely affected by the contract ratio in the boundary layer. The fibers orientation in the near semi-dilute phase is lower than that in the dilute phase near the outlet, which may be the result of the hydrodynamic contact lubrication between fibers. The orientation distribution and concentration of the fibers change the viscous flow mechanism of the suspension microscopically, which makes a velocity profile vary with the phase concentration. The velocity profile of the approaching semi-dilute phase sublayer is higher than that of the dilute and semi-dilute phases on the central streamline and in the viscous bottom layer, showing weak drag reduction while the situation is opposite on the logarithmic layer of the boundary layer. The relevant research can provide a process strategy for fiber orientation optimization and rheological control in the industrial applications of suspension.

scholarly journals High level production of laccases and peroxidases from the newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111 in a stirred-tank bioreactor in response to different carbon and nitrogen sources

2018 ◽  
Vol 69 ◽  
pp. 1-11 ◽  
Author(s):  
Willian Daniel Hahn Schneider ◽  
Roselei Claudete Fontana ◽  
Simone Mendonça ◽  
Félix Gonçalves de Siqueira ◽  
Aldo José Pinheiro Dillon ◽  
...  
Keyword(s):  
Nitrogen Sources ◽  
White Rot ◽  
Stirred Tank ◽  
Carbon And Nitrogen Sources ◽  
Stirred Tank Bioreactor ◽  
Carbon And Nitrogen ◽  
High Level ◽  
Level Production

Growth Conditions of a Thermophilic Geobacillus sp. as an Enhanced Oil Recovery Material

2012 ◽  
Vol 496 ◽  
pp. 457-460
Author(s):  
Xiang Ping Kong
Keyword(s):  
Enhanced Oil Recovery ◽  
Bacterial Growth ◽  
Oil Recovery ◽  
Nitrogen Sources ◽  
Growth Conditions ◽  
Good Growth ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

The growth conditions of a Geobacillus sp. were investigated by single-factor experiments. The strain was strictly aerobic bacterium, and could grow on hydrocarbons as the sole carbon source. The optimum carbon and nitrogen sources were 3.0% sucrose and 0.20% KNO3, respectively. The range of temperature, salinity and pH for the bacterial growth was 35-70 °C, 0-10% NaCl and 5.5-9.5, and good growth was obtained at 35-65 °C, 0.5-8% NaCl and 6.0-9.0, respectively. Particularly, the optimum temperature for the bacterial growth was between 50 °C and 60 °C. The strain had wide adaptability to the extreme conditions, and may be potentially applied to microbial enhanced oil recovery and oil-waste bioremediation technology.

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