Production of Microbial Exopolysaccharide by Cost-effective Medium Optimization Method

2019 ◽  
pp. 1-12
Author(s):  
I. G. Nwosu ◽  
G. O. Abu ◽  
K. O. Agwa
Keyword(s):  
Sweet Potato ◽  
Fermentation Broth ◽  
Maximum Yield ◽  
Optimization Method ◽  
Fermentation Medium ◽  
Agricultural Wastes ◽  
Efficient Production ◽  
Total Carbohydrate ◽  
Carbon And Nitrogen ◽  
Extract Medium

Microbial exopolysaccharide (EPS) emerged as a fast and high yielding sustainable polymeric substance which can be used as an alternative to synthetic polymer in industry. In this study, the influence of various nutritional and environmental factors of fermentation medium on bacterial growth and EPS production was evaluated by one factor at a time optimization. Efficient production medium was chosen from four different basal media and its carbon and nitrogen substrates were varied among organic and inorganic sources. Feasibility of bacterial utilization of some agricultural wastes as carbon and nitrogen sources to synthesize exopolysaccharide was compared. Carbon source of the fermentation medium was replaced with hydrolysates of sugarcane baggasse (SCB), sweet potato peels (SPP) or ripe plantain peels (RPP) at various concentrations while the nitrogen substrates was replaced with extracts of poultry droppings (PP), groundnut pod (GP) or beans bran (BB). Response results observed from single factor optimization were explored as center points to design a model for Response Surface Methodology study. Cell growth was determined from the biomass population of the fermentation broth after 5days of incubation in a rotary shaker at 120 rpm at 30oC. EPS was precipitated with pre-chilled ethanol (at 4oC) from cell-free broth and overnight incubation at 4oC. Total carbohydrate content was estimated by phenol-sulphuric acid method. Result obtained showed that 2% concentration Hydrolysate of SPP containing medium gave maximum yield of 2.26g EPS/l of the medium as compared to sucrose containing medium with yield of 1.25 g EPS/l of the medium while highest production yield of 9.46 gEPS/l of the medium was obtained from 10 g/l BB extract medium as compared to yeast extract medium (5.41 gEPS/l). Results indicated that agricultural wastes such as sweet potato peel hydrolysate and bean bran extract could be developed as inexpensive alternative route to synthesize EPS from bacteria than inorganic substrates.

scholarly journals Production of Microbial Exopolysaccharide by Cost-effective Medium Opimization Method

2019 ◽  
pp. 1-13
Author(s):  
I. G. Nwosu ◽  
G. O. Abu ◽  
K. O. Agwa
Keyword(s):  
Sweet Potato ◽  
Fermentation Broth ◽  
Maximum Yield ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Agricultural Wastes ◽  
Efficient Production ◽  
Total Carbohydrate ◽  
Carbon And Nitrogen ◽  
Extract Medium

Microbial exopolysaccharide (EPS) emerged as a fast and high yielding sustainable polymeric substance which can be used as an alternative to synthetic polymer in industry. In this study, the influence of various nutritional and environmental factors of fermentation medium on bacterial growth and EPS production was evaluated by one factor at a time optimization. Efficient production medium was chosen from four different basal media and its carbon and nitrogen substrates were varied among organic and inorganic sources. Feasibility of bacterial utilization of some agricultural wastes as carbon and nitrogen sources to synthesize exopolysaccharide was compared. Carbon source of the fermentation medium was replaced with hydrolysates of sugarcane baggasse (SCB), sweet potato peels (SPP) or ripe plantain peels (RPP) at various concentrations while the nitrogen substrates was replaced with extracts of poultry droppings (PP), groundnut pod (GP) or beans bran (BB). Response results observed from single factor optimization were explored as center points to design a model for Response Surface Methodology study. Cell growth was determined from the biomass population of the fermentation broth after 5 days of incubation in a rotary shaker at 120 rpm at 30ºC. EPS was precipitated with pre-chilled ethanol (at 4ºC) from cell-free broth and overnight incubation at 4ºC. Total carbohydrate content was estimated by phenol-sulphuric acid method. Result obtained showed that 2% concentration Hydrolysate of SPP containing medium gave maximum yield of 2.26 g EPS/l of the medium as compared to sucrose containing medium with yield of 1.25 g EPS/l of the medium while highest production yield of 9.46 gEPS/l of the medium was obtained from 10 g/l BB extract medium as compared to yeast extract medium (5.41 gEPS/l). Results indicated that agricultural wastes such as sweet potato peel hydrolysate and bean bran extract could be developed as inexpensive alternative route to synthesize EPS from bacteria than inorganic substrates.

scholarly journals Optimization of Cultural Conditions for Production of Antibacterial Metabolites from Streptomyces coelicoflavus BC 01

2015 ◽  
Vol 7 (2) ◽  
pp. 151-159
Author(s):  
Kothagorla Venkata RAGHAVA RAO ◽  
Dadi BHASKARA RAO ◽  
Botcha SATYANARAYANA ◽  
Tamanam RAGHAVA RAO
Keyword(s):  
Andhra Pradesh ◽  
Maximum Yield ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Carbon And Nitrogen ◽  
Mangrove Soil ◽  
Cultural Conditions ◽  
Bean Meal ◽  
Metabolites Production ◽  
Soya Bean Meal

The aim of the present study was to optimize various cultural conditions for the production of antibacterial metabolites by Streptomyces coelicoflavus BC 01 isolated from mangrove soil, Visakhapatnam, Andhra Pradesh, India. The effect of various factors such as carbon and nitrogen sources, different concentrations of NaCl and K2HPO4, different temperature, pH, incubation time and agitation on antibacterial metabolites production were studied. The production of antibacterial metabolites by the isolate Streptomyces coelicoflavus BC 01 was greatly influenced by the cultural conditions. Glucose (1.2%) and soya bean meal (1%) seemed to be the best carbon and nitrogen source respectively, followed by NaCl (1%) and K2HPO4 (0.25%). Maximum production of antibacterial metabolites was observed at a temperature of 30 °C, with pH 7.2, at 160 rpm for 96 hrs. These optimized parameters can be further useful to design a fermentation medium to achieve maximum yield of antibacterial metabolites from Streptomyces coelicoflavus BC 01.

scholarly journals Screening of Penicillium Species and Optimisation of Culture Conditions for the Production of Ergot Alkaloids Using Surface Culture Fermentation Process

2015 ◽  
Vol 58 (1) ◽  
pp. 23-29
Author(s):  
Memuna Ghafoor Shahid ◽  
Muhammad Nadeem
Keyword(s):  
Maximum Yield ◽  
Ergot Alkaloids ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Culture Conditions ◽  
Fungal Species ◽  
Surface Culture ◽  
Penicillium Species ◽  
Carbon And Nitrogen ◽  
Culture Process

The present study deals with the screening of fungal species and suitable fermentation medium for the production of ergot alkaloids. Various species of genus Penicillium were grown on differentfermentation media by employing surface culture fermentation technique to achieve the most suitable medium and the best Penicillium sp. The results showed that medium M5 gave maximum yield withPenicillium commune. Different culture conditions such as effect of different carbon and nitrogen sources, their concentration levels, different pH values and sizes of inoculum on the production of ergot alkaloids were also studied to improve the yield. Maximum production of ergot alkaloids (4.32 mg/L) was achieved with 15 mL spore suspension at pH 5 in fermentation medium containing 35% (w/v) sucrose. All these  results indicate that culture conditions are very much crucial to improve the yield of ergot alkaloids produced by Penicillium commune through surface culture process. 

scholarly journals A highly thermotolerant laccase produced by Cerrena unicolor strain CGMCC 5.1011 for complete and stable malachite green decolorization

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yanhua Yao ◽  
Guimei Zhou ◽  
Yonghui Lin ◽  
Xinqi Xu ◽  
Jie Yang
Keyword(s):  
Malachite Green ◽  
Fermentation Broth ◽  
Nitrogen Sources ◽  
Life Time ◽  
Industrial Applications ◽  
Dynamics Simulation ◽  
Carbon And Nitrogen ◽  
Cdna Sequences ◽  
Cerrena Unicolor ◽  
Future Work

Abstract Laccases are a class of multi-copper oxidases with important industrial values. A thermotolerant laccase produced by a basidiomycete fungal strain Cerrena unicolor CGMCC 5.1011 was studied. With glycerin and peptone as the carbon and nitrogen sources, respectively, a maximal laccase activity of 121.7 U/mL was attained after cultivation in the shaking flask for 15 days. Transcriptomics analysis revealed an expressed laccase gene family of 12 members in C. unicolor strain CGMCC 5.1011, and the gene and cDNA sequences were cloned. A glycosylated laccase was purified from the fermentation broth of Cerrena unicolor CGMCC 5.1011 and corresponded to Lac2 based on MALDI-TOF MS/MS identification. Lac2 was stable at pH 5.0 and above, and was resistant to organic solvents. Lac2 displayed remarkable thermostability, with half-life time of 1.67 h at 70 ºC. Consistently, Lac2 was able to completely decolorize malachite green (MG) at high temperatures, whereas Lac7 from Cerrena sp. HYB07 resulted in accumulation of colored MG transformation intermediates. Molecular dynamics simulation of Lac2 was conducted, and possible mechanisms underlying Lac2 thermostability were discussed. The robustness of C. unicolor CGMCC 5.1011 laccase would not only be useful for industrial applications, but also provide a template for future work to develop thermostable laccases.

Accumulation of carbon and nitrogen compounds in sweet potato plants grown under deficiency of N, P, or K nutrients

1995 ◽  
Vol 41 (3) ◽  
pp. 557-566 ◽  
Author(s):  
Mitsuru Osaki ◽  
Hiroyuki Ueda ◽  
Takuro Shinano ◽  
Hirokazu Matsui ◽  
Toshiaki Tadano
Keyword(s):  
Sweet Potato ◽  
Nitrogen Compounds ◽  
Carbon And Nitrogen ◽  
Potato Plants

scholarly journals Pengembangan Hybrid Tepung Ubi Jalar Kaya Antioksidan

2019 ◽  
Vol 10 (2) ◽  
pp. 153
Author(s):  
Sefanadia Putri
Keyword(s):  
Antioxidant Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Sweet Potato ◽  
Block Design ◽  
Acetobacter Xylinum ◽  
Potato Flour ◽  
Total Carbohydrate ◽  
Sweet Potato Flour ◽  
Purple Sweet Potato

<p>Sweet potato is one type of tubers found in Indonesia. <em>Hybrid</em> of sweet potato flour is obtained from the modification of the processing by fermentation using lactic acid bacteria. The purpose of this study was to determine the nutritional and antioxidant content of various types of <em>hybrid</em> sweet potato flour. This research is an experimental laboratory with a true-experiment design. The experiment was arranged in a complete randomized block design (RCB) with three replications. The treatments are 6 types of sweet potatoes which include purple sweet potato Var.Antin 1, purple sweet potato val.local, white sweet potato, orange sweet potato, Japanese purple sweet potato Var.Ayamurasaki and cilembu sweet potato. The study included the fermentation process using lactic acid bacteria (Acetobacter xylinum) addition concentration of 5%, siege, analysis of nutrient content (water, ash, protein, fat, fiber and carbohydrates) and antioxidant activity of various types of <em>hybrid</em> sweet potato flour. The results showed that various types of <em>hybrid</em> sweet potato flour significantly affect the nutritional content which includes water content, ash content, fat content, protein content, fiber content and total carbohydrate. The result of antioxidant activity in various types of <em>hybrid</em> sweet potato flour after fermentation treatment in a concentration of 5% Acetobacter xylinum ranged from 29,383-53.7929%. The lowest value obtained at cilembu <em>hybrid</em> sweet potato flour and the highest value was obtained from the Japanese <em>hybrid</em> sweet potato flour var.Ayamurasaki.</p>

scholarly journals Effect of potassium and zinc on growth, yield and economics of sweet potato (Ipomoea batatas L.) cv. CO-34

2017 ◽  
Vol 9 (1) ◽  
pp. 291-297
Author(s):  
Pravin Singh ◽  
Kavita Aravindakshan ◽  
I. B. Maurya ◽  
Jitendra Singh ◽  
Bhim Singh ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Tuber Yield ◽  
Foliar Application ◽  
Block Design ◽  
Maximum Yield ◽  
Growth Yield ◽  
Cost Ratio ◽  
Average Weight ◽  
Four Levels

A field experiment was conducted to study the effect of different levels of potassium and zinc on growth, yield and economics of sweet potato. The experiment was laid out on clay and loam soil by adopting randomized block design with factorial technique (FRBD). The sixteen treatments consisted of combination of four levels of po-tassium (0, 80, 100 and 120 kg/ha through muriate of potash and four levels of foliar zinc (control i.e. water spray, 10, 20 and 30ppm) through zinc sulphate. The individual application of potassium 120 kg K2O/ha significantly in-creased the number of tubers per plant (4.60), average weight of tuber (275.31 g), length of tuber (16.77 cm), diam-eter of tuber (5.69 cm), tuber yield per plot (9.71 kg), tuber yield per hectare (49.04 t) respectively as compared to control. With the foliar application of zinc (30 ppm) significant increase in number of tubers per plant (4.18), average weight of tuber (234.73 g), length of tuber (18.12 cm), diameter of tuber (5.16 cm), tuber yield per plot (8.33 kg) and tuber yield per hectare (42.05 t) was recordedas compared to control. The treatment combination (120 kg K2O+30ppm Zn) recorded the maximum yield parameters i.e. chlorophyll content (37.00 mg/100 g), average weight of tuber (302.17 g), length of tuber (19.82 cm), diameter of tuber (5.97 cm), maximum tuber yield per plot (11.02 kg), tuber yield per hectare (55.67 t) and benefit-cost ratio (B: C ratio) of 4.22:1.While, the treatment (120 kg K2O+30ppm Zn) had the maximum number of tuber (4.86), minimum number of tuber was recorded in control. From the experi-ment, it appeared that application of potassium and zinc can be used to improve yield and higher net monetary re-turns of sweet potato.

scholarly journals Quality Characteristics of Nutri-fried Noodles Made from Sweet Potato Tubers and Tops Ipomea batatas

2016 ◽  
Vol 24 (1) ◽  
Author(s):  
Geraldine F. De Jesus ◽  
Shirley G. Dicen ◽  
Maria Crisanta M. Jarque
Keyword(s):  
Shelf Life ◽  
Sweet Potato ◽  
Sugar Content ◽  
Quality Characteristics ◽  
Second Phase ◽  
Potato Tubers ◽  
Total Carbohydrate ◽  
Ipomea Batatas ◽  
Microbial Analysis ◽  
Total Fat

Noodles made from sweet potato can be a healthier alternative to commercially produced ones since it is rich in vitamins and minerals. The product has no artificial preservatives, and the sweetness of its taste comes naturally from the sugar content of the Camote tubers. The project is the second phase of a study on the development of nutri-noodles made from the tubers and tops of sweet potato.  The study determined the physicochemical and microbial analysis and shelf-life of the product and verify its acceptability through quantitative testing. The sample was submitted to Department of Science and Technology Region V for analysis and the results were: 6.53% moisture, 0.13% free fatty acids and <150 CFU/g of yeast and mold count. Moreover, nutrition facts were also computed, Calories is 231, Total Fat= 4%, Cholesterol= 0%, Sodium= 4%, Total Carbohydrate= 6%, Dietary Fiber= 28%, Magnesium =13% and Vitamin B6= 12%. The shelf-life of the product is six months stored at room temperature. The Nutri-fried noodles were served with vegetable toppings, and as rated by the panelists the result was liked very much. After analyzing the quality characteristics of the product, it is recommended that the sample is ready for production and marketing.

Optimization of Virgin Coconut Oil (VCO) Production with Diffuser Type Aeration Method

2021 ◽  
Vol 6 (4) ◽  
pp. 139-143
Author(s):  
Nyoman Sri Widari ◽  
Rahaju Saraswati ◽  
Bambang Sutejo
Keyword(s):  
Fatty Acid ◽  
Flow Rate ◽  
Acid Content ◽  
Air Flow ◽  
Fatty Acid Content ◽  
Maximum Yield ◽  
Coconut Oil ◽  
Efficient Production ◽  
Air Flow Rate ◽  
Virgin Coconut Oil

Virgin coconut oil (VCO) is pure coconut oil with low free fatty acid content and high lauric acid content. VCO has been produced in many home industries where the yield obtained is still very small. So, the researchers tried to find a more efficient production process so that they could obtain high process yields. Researchers made VCO using the aeration method with a diffuser type aerator. The experimental variables were air flow rate (L / min): 0.6; 1.8; 4.0; 4,5 and the duration of the aeration process (hours): 2; 3; 4; 5, by using coconut milk from 1 kg of grated coconut in 1 liter of water, the maximum yield is 33.5% at an air flow rate of 4.0 L / min with an aeration time of 4 hours. The quality of VCO obtained were: water content of 0.02%; iodine number 5,54%; peroxidation number 1.36 g.iod / 100 g; 0.19% free fatty acids; saturated fatty acid content of 93.71% and unsaturated fatty acid 6.24% and the color is very clear. The quality test results obtained are in accordance with the quality standards required by SNI 7381-2008.

Study on optimization of mechanical peeling for sweet potato

2020 ◽  
Vol 44 (01) ◽  
pp. 1-7
Author(s):  
P Vithu ◽  
Sanjaya K. Dash ◽  
Kalpana Rayaguru ◽  
Uma Sankar Pal
Keyword(s):  
Sweet Potato ◽  
Research Work ◽  
Maximum Yield ◽  
Optimal Conditions ◽  
Performance Indices ◽  
Rotating Disc ◽  
Minimum Loss ◽  
Material Loss ◽  
Commercial Processing

The aim of this research work was to optimize the machine parameters of a commercially available tuber peeler for peeling of sweet potato to get maximum yield with minimum loss. The process was proposed for different parameters such as peeling efficiency, material loss and peeling effectiveness at different speeds of rotating disc (300, 400 and 500 rev/min or 5.4, 7.2 and 9 m/s), peeling durations (20, 40 and 60 s) and batch loads of 1 and 2 kg. The study revealed that the peeling parameters were greatly influenced by the speed of rotating disc, peeling duration and batch loads. The optimal conditions for peeling sweet potato were a peeling duration of 40 s and speed of rotating disc of 500 rev/min 2 kg batch loads. The corresponding peeling efficiency, peeling effectiveness and material loss were 83.8±2.0%, 74.2±1.3% and 11.45±0.71%, respectively. Further, the study showed that a batch load of 2 kg was suitable for commercial processing of sweet potato than 1 kg because of the better performance indices of peeling.

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