scholarly journals Optimization of Cultural Conditions for Production of Antibacterial Metabolites from Streptomyces coelicoflavus BC 01

2015 ◽  
Vol 7 (2) ◽  
pp. 151-159
Author(s):  
Kothagorla Venkata RAGHAVA RAO ◽  
Dadi BHASKARA RAO ◽  
Botcha SATYANARAYANA ◽  
Tamanam RAGHAVA RAO
Keyword(s):  
Andhra Pradesh ◽  
Maximum Yield ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Carbon And Nitrogen ◽  
Mangrove Soil ◽  
Cultural Conditions ◽  
Bean Meal ◽  
Metabolites Production ◽  
Soya Bean Meal

The aim of the present study was to optimize various cultural conditions for the production of antibacterial metabolites by Streptomyces coelicoflavus BC 01 isolated from mangrove soil, Visakhapatnam, Andhra Pradesh, India. The effect of various factors such as carbon and nitrogen sources, different concentrations of NaCl and K2HPO4, different temperature, pH, incubation time and agitation on antibacterial metabolites production were studied. The production of antibacterial metabolites by the isolate Streptomyces coelicoflavus BC 01 was greatly influenced by the cultural conditions. Glucose (1.2%) and soya bean meal (1%) seemed to be the best carbon and nitrogen source respectively, followed by NaCl (1%) and K2HPO4 (0.25%). Maximum production of antibacterial metabolites was observed at a temperature of 30 °C, with pH 7.2, at 160 rpm for 96 hrs. These optimized parameters can be further useful to design a fermentation medium to achieve maximum yield of antibacterial metabolites from Streptomyces coelicoflavus BC 01.

scholarly journals Screening of Penicillium Species and Optimisation of Culture Conditions for the Production of Ergot Alkaloids Using Surface Culture Fermentation Process

2015 ◽  
Vol 58 (1) ◽  
pp. 23-29
Author(s):  
Memuna Ghafoor Shahid ◽  
Muhammad Nadeem
Keyword(s):  
Maximum Yield ◽  
Ergot Alkaloids ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Culture Conditions ◽  
Fungal Species ◽  
Surface Culture ◽  
Penicillium Species ◽  
Carbon And Nitrogen ◽  
Culture Process

The present study deals with the screening of fungal species and suitable fermentation medium for the production of ergot alkaloids. Various species of genus Penicillium were grown on differentfermentation media by employing surface culture fermentation technique to achieve the most suitable medium and the best Penicillium sp. The results showed that medium M5 gave maximum yield withPenicillium commune. Different culture conditions such as effect of different carbon and nitrogen sources, their concentration levels, different pH values and sizes of inoculum on the production of ergot alkaloids were also studied to improve the yield. Maximum production of ergot alkaloids (4.32 mg/L) was achieved with 15 mL spore suspension at pH 5 in fermentation medium containing 35% (w/v) sucrose. All these  results indicate that culture conditions are very much crucial to improve the yield of ergot alkaloids produced by Penicillium commune through surface culture process. 

scholarly journals Production of Microbial Exopolysaccharide by Cost-effective Medium Opimization Method

2019 ◽  
pp. 1-13
Author(s):  
I. G. Nwosu ◽  
G. O. Abu ◽  
K. O. Agwa
Keyword(s):  
Sweet Potato ◽  
Fermentation Broth ◽  
Maximum Yield ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Agricultural Wastes ◽  
Efficient Production ◽  
Total Carbohydrate ◽  
Carbon And Nitrogen ◽  
Extract Medium

Microbial exopolysaccharide (EPS) emerged as a fast and high yielding sustainable polymeric substance which can be used as an alternative to synthetic polymer in industry. In this study, the influence of various nutritional and environmental factors of fermentation medium on bacterial growth and EPS production was evaluated by one factor at a time optimization. Efficient production medium was chosen from four different basal media and its carbon and nitrogen substrates were varied among organic and inorganic sources. Feasibility of bacterial utilization of some agricultural wastes as carbon and nitrogen sources to synthesize exopolysaccharide was compared. Carbon source of the fermentation medium was replaced with hydrolysates of sugarcane baggasse (SCB), sweet potato peels (SPP) or ripe plantain peels (RPP) at various concentrations while the nitrogen substrates was replaced with extracts of poultry droppings (PP), groundnut pod (GP) or beans bran (BB). Response results observed from single factor optimization were explored as center points to design a model for Response Surface Methodology study. Cell growth was determined from the biomass population of the fermentation broth after 5 days of incubation in a rotary shaker at 120 rpm at 30ºC. EPS was precipitated with pre-chilled ethanol (at 4ºC) from cell-free broth and overnight incubation at 4ºC. Total carbohydrate content was estimated by phenol-sulphuric acid method. Result obtained showed that 2% concentration Hydrolysate of SPP containing medium gave maximum yield of 2.26 g EPS/l of the medium as compared to sucrose containing medium with yield of 1.25 g EPS/l of the medium while highest production yield of 9.46 gEPS/l of the medium was obtained from 10 g/l BB extract medium as compared to yeast extract medium (5.41 gEPS/l). Results indicated that agricultural wastes such as sweet potato peel hydrolysate and bean bran extract could be developed as inexpensive alternative route to synthesize EPS from bacteria than inorganic substrates.

Production of Microbial Exopolysaccharide by Cost-effective Medium Optimization Method

2019 ◽  
pp. 1-12
Author(s):  
I. G. Nwosu ◽  
G. O. Abu ◽  
K. O. Agwa
Keyword(s):  
Sweet Potato ◽  
Fermentation Broth ◽  
Maximum Yield ◽  
Optimization Method ◽  
Fermentation Medium ◽  
Agricultural Wastes ◽  
Efficient Production ◽  
Total Carbohydrate ◽  
Carbon And Nitrogen ◽  
Extract Medium

Microbial exopolysaccharide (EPS) emerged as a fast and high yielding sustainable polymeric substance which can be used as an alternative to synthetic polymer in industry. In this study, the influence of various nutritional and environmental factors of fermentation medium on bacterial growth and EPS production was evaluated by one factor at a time optimization. Efficient production medium was chosen from four different basal media and its carbon and nitrogen substrates were varied among organic and inorganic sources. Feasibility of bacterial utilization of some agricultural wastes as carbon and nitrogen sources to synthesize exopolysaccharide was compared. Carbon source of the fermentation medium was replaced with hydrolysates of sugarcane baggasse (SCB), sweet potato peels (SPP) or ripe plantain peels (RPP) at various concentrations while the nitrogen substrates was replaced with extracts of poultry droppings (PP), groundnut pod (GP) or beans bran (BB). Response results observed from single factor optimization were explored as center points to design a model for Response Surface Methodology study. Cell growth was determined from the biomass population of the fermentation broth after 5days of incubation in a rotary shaker at 120 rpm at 30oC. EPS was precipitated with pre-chilled ethanol (at 4oC) from cell-free broth and overnight incubation at 4oC. Total carbohydrate content was estimated by phenol-sulphuric acid method. Result obtained showed that 2% concentration Hydrolysate of SPP containing medium gave maximum yield of 2.26g EPS/l of the medium as compared to sucrose containing medium with yield of 1.25 g EPS/l of the medium while highest production yield of 9.46 gEPS/l of the medium was obtained from 10 g/l BB extract medium as compared to yeast extract medium (5.41 gEPS/l). Results indicated that agricultural wastes such as sweet potato peel hydrolysate and bean bran extract could be developed as inexpensive alternative route to synthesize EPS from bacteria than inorganic substrates.

scholarly journals Biodegradation of Atrazine by Agrobacterium radiobacter J14a and Use of This Strain in Bioremediation of Contaminated Soil

1998 ◽  
Vol 64 (9) ◽  
pp. 3368-3375 ◽  
Author(s):  
J. K. Struthers ◽  
K. Jayachandran ◽  
T. B. Moorman
Keyword(s):  
Indigenous Population ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Agrobacterium Radiobacter ◽  
Carbon And Nitrogen ◽  
Cultural Conditions ◽  
Herbicide Atrazine ◽  
Lag Times ◽  
Chemical Distribution ◽  
Atrazine Degradation

ABSTRACT We examined the ability of a soil bacterium, Agrobacterium radiobacter J14a, to degrade the herbicide atrazine under a variety of cultural conditions, and we used this bacterium to increase the biodegradation of atrazine in soils from agricultural chemical distribution sites. J14a cells grown in nitrogen-free medium with citrate and sucrose as carbon sources mineralized 94% of 50 μg of [14C-U-ring]atrazine ml−1 in 72 h with a concurrent increase in the population size from 7.9 × 105 to 5.0 × 107 cells ml−1. Under these conditions cells mineralized the [ethyl-14C]atrazine and incorporated approximately 30% of the 14C into the J14a biomass. Cells grown in medium without additional carbon and nitrogen sources degraded atrazine, but the cell numbers did not increase. Metabolites produced by J14a during atrazine degradation include hydroxyatrazine, deethylatrazine, and deethyl-hydroxyatrazine. The addition of 105 J14a cells g−1 into soil with a low indigenous population of atrazine degraders treated with 50 and 200 μg of atrazine g−1soil resulted in two to five times higher mineralization than in the noninoculated soil. Sucrose addition did not result in significantly faster mineralization rates or shorten degradation lag times. However, J14a introduction (105 cells g−1) into another soil with a larger indigenous atrazine-mineralizing population reduced the atrazine degradation lag times below those in noninoculated treatments but did not generally increase total atrazine mineralization.

A comparison of sources of supplementary nitrogen for young cattle receiving fibre-rich diets

1980 ◽  
Vol 95 (3) ◽  
pp. 687-695 ◽  
Author(s):  
T. Smith ◽  
Valerie J. Broster ◽  
R. E. Hill
Keyword(s):  
Nitrogen Retention ◽  
Live Weight ◽  
Nitrogen Sources ◽  
Soya Bean ◽  
Nitrogen Concentrations ◽  
Feeding Trials ◽  
Bean Meal ◽  
Young Cattle ◽  
Soya Bean Meal ◽  
Protein Supply

SUMMARYFishmeal, soya-bean meal and urea were compared as nitrogen sources in diets rich in fibre for yearling cattle, using feeding trials and digestibility and nitrogen retention studies. All animals were individually fed. Diets supplemented with fishmeal supported the highest rates of daily live-weight gain and nitrogen retention. There was no response in dry-matter intake and digestibility from extra nitrogen, either from fishmeal or urea, when the crude protein of the diet was 8·5% or over, and a small response in digestibility when soya-bean meal was used.Molar proportions of VFA, rumen NH3-N concentrations and blood urea nitrogen concentrations were all affected by both amount and source of nitrogen supplementation. Multiple regression analysis showed the undegradable protein supply to be more critical with high than with low fibre diets.

scholarly journals Production of Phytase by three thermophilic fungi

2021 ◽  
Vol 23 (1) ◽  
Author(s):  
Ranjith Kumar R ◽  
Rajendar Reddy ◽  
Girisham S ◽  
Reddy S.M.
Keyword(s):  
Carbon Source ◽  
Nitrogen Sources ◽  
The Other ◽  
Thermomyces Lanuginosus ◽  
Thermophilic Fungi ◽  
Carbon And Nitrogen Sources ◽  
Phytase Production ◽  
Carbon And Nitrogen ◽  
Cultural Conditions ◽  
Other Hand

Production of phytase by three thermophilic fungi, Thermomyces lanuginosus, Talaromyces luteus and Rhizomucor pusillus under different cultural conditions was assessed. Temperature of 45°C, pH-6.0 were optimum for phytase production by the all three fungi under investigation . Carbon and nitrogen sources for production of phytases by the three thermophilic fungi varied with the fungus. When T. lanuginosus opted for D-glucose followed by D-fructose, T. luteus preferred D-glucose, D-mannose and mannitol for production a phytase. On the other hand, R. pusillus produced maximum phytase during its growth on mannitol and maltose as carbon source. L- asparagine, L- arginine and L-asparatic acid were preferred nitrogen sources for production of phytase by T. lanuginosus. On the other hand T. luteus, opted for L- asparagine, L-glutamic acid and L- glycine for the activity of phytase. R. pusillus produced maximum phytase in medium containing L-argine, L-asparagine and L- asparatic acid.

Hydrolysis of Corncob Residue by Enzymes from Fungi C1067 and the Products Analysis by Capillary Electrophoresis

2011 ◽  
Vol 145 ◽  
pp. 68-72
Author(s):  
Jian Lu Ma ◽  
Xiao Min Fang ◽  
Xin Jiong Guo ◽  
Huan Fei Liu ◽  
Gui Fu Dai ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Corn Steep Liquor ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Citrate Buffer ◽  
Carbon And Nitrogen ◽  
Products Analysis ◽  
Steep Liquor ◽  
Hydrolysis Of ◽  
Assay Conditions

To achieve high enzymatic hydrolytic efficiency of corncob residue, one strain, Trichoderma C1067, with the enzyme activity of corncob residue as high as 4.5g/L glucose liberated per hour in 0.5mL crude cellulase and 0.5mL citric acid /sodium citrate buffer under the assay conditions, was carefully studied. The optimal carbon and nitrogen sources used in the fermentation medium are as follows: corncob residues 2.0%, wheat bran 1.5%, corncob 0.5%, rice straw 0.5%, peptone 0.1% and corn steep liquor 4%. The optimal conditions of saccharification for corncob are 55°C and pH 4.0. The sugars gained in the hydrolysate consist of glucose 4.1% and xylose 0.4% after enzymolysis for 72h, tested by capillary electrophoresis.

Experimental Optimization of Green Hydrogen Production from Phototrophic Bacteria Rhodobacter sphaeroides

2019 ◽  
Vol 12 (2) ◽  
pp. 98-109
Author(s):  
Swetha Garimella ◽  
Archana Vimal ◽  
Ramchander Merugu ◽  
Awanish Kumar
Keyword(s):  
Hydrogen Production ◽  
Rhodobacter Sphaeroides ◽  
Energy Demand ◽  
Nitrogen Sources ◽  
Sewage Water ◽  
Quadratic Model ◽  
Carbon And Nitrogen ◽  
Cultural Conditions ◽  
Green Route ◽  
Production Of Hydrogen

Background and Objective: This study utilizes Rhodobacter sphaeroides bacteria for the photoproduction of hydrogen under various cultural conditions. R. sphaeroides was isolated from sewage water. We have examined different carbon and nitrogen sources for hydrogen production and further established the conditions for optimum hydrogen production by R. sphaeroides. Methods: The cumulative hydrogen produced by the bacteria at various intervals of time was measured using a Gas Chromatograph. Initially, by classical one factor at a time method, it was found that Benzoate and Glycine promote higher amounts of hydrogen production under anaerobic light conditions after 96 h. Results: The production was also observed to be enhanced in the presence of growth factors B12. Further, the Response Surface Methodology (RSM) was employed to optimize the hydrogen production. The first level of optimization was done using Box-Behnken Design (BBD) followed by Central Composite Design (CCD) method. The maximum production of hydrogen achieved by BBD and CCD was 6.8 ml/30 ml and 8.12 ml/30 ml, respectively. The significant model predicted is a quadratic model with R2 value 0.9459. Conclusion: Moreover, work presented here suggests an environment-friendly approach of harvesting H2, which could meet energy demand as clean fuel via the green route.

scholarly journals Biosurfactant production by fungi as a sustainable alternative

2018 ◽  
Vol 85 (0) ◽  
Author(s):  
Artemisia Carla Santos da Silva ◽  
Patrícia Nunes dos Santos ◽  
Thayse Alves Lima e Silva ◽  
Rosileide Fontenele Silva Andrade ◽  
Galba Maria Campos-Takaki
Keyword(s):  
Metabolic Pathways ◽  
Nitrogen Sources ◽  
Health Food ◽  
Carbon And Nitrogen Sources ◽  
Biotechnological Potential ◽  
Carbon And Nitrogen ◽  
Cultural Conditions ◽  
Environmentally Safe ◽  
Small Change ◽  
Food Processes

ABSTRACT: A wide variety of bacteria is far more exploited than fungi as biosurfactants (BS) or bioemulsifiers (BE), using renewable sources. BS are considered to be environmentally safe and offer advantages over synthetic surfactants. However, the BS yield depends largely on the metabolic pathways of the microorganisms and the nutritional medium. The production of BS or BE uses several cultural conditions, in which a small change in carbon and nitrogen sources affects the quantity of BS or BE produced. The type and quantity of microbial BS or BE produced depend mainly on the producer organism, and factors such as carbon and nitrogen sources, trace elements, temperature and aeration. The diversity of BS or BE makes it interesting to apply them in the pharmaceutical and cosmetics industries, agriculture, public health, food processes, detergents, when treating oily residues, environmental pollution control and bioremediation. Thus, this paper reviews and addresses the biotechnological potential of yeasts and filamentous fungi for producing, characterizing and applying BS or BE.

scholarly journals Yarrowia lipolyticaand Its Multiple Applications in the Biotechnological Industry

2014 ◽  
Vol 2014 ◽  
pp. 1-14 ◽  
Author(s):  
F. A. G. Gonçalves ◽  
G. Colen ◽  
J. A. Takahashi
Keyword(s):  
Carbon Sources ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Biotechnological Potential ◽  
Carbon And Nitrogen ◽  
Intracellular Lipids ◽  
Metabolites Production ◽  
Growing Conditions ◽  
Fermentation Parameters

Yarrowia lipolyticais a nonpathogenic dimorphic aerobic yeast that stands out due to its ability to grow in hydrophobic environments. This property allowed this yeast to develop an ability to metabolize triglycerides and fatty acids as carbon sources. This feature enables using this species in the bioremediation of environments contaminated with oil spill. In addition,Y. lipolyticahas been calling the interest of researchers due to its huge biotechnological potential, associated with the production of several types of metabolites, such as bio-surfactants,γ-decalactone, citric acid, and intracellular lipids and lipase. The production of a metabolite rather than another is influenced by the growing conditions to whichY. lipolyticais subjected. The choice of carbon and nitrogen sources to be used, as well as their concentrations in the growth medium, and the careful determination of fermentation parameters, pH, temperature, and agitation (oxygenation), are essential for efficient metabolites production. This review discusses the biotechnological potential ofY. lipolyticaand the best growing conditions for production of some metabolites of biotechnological interest.

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