scholarly journals Design and Development of Mini-pulsar for Real-Time Bioelectric Experiment

2021 ◽  
pp. 9-16
Author(s):  
Gyanendra Kumar ◽  
R Sarathi ◽  
Archana Sharma
Keyword(s):  
Electric Field ◽  
Real Time ◽  
Eukaryotic Cells ◽  
Experimental Setup ◽  
Single Shot ◽  
Nanosecond Duration ◽  
Reversible Electroporation ◽  
Intracellular Organelles ◽  
Apoptotic Pathways ◽  
Very High

Investigation of the effect of pulsed electric field on eukaryotic cells has been of interestto biomedical scientists and researchers since a few decades. Reversible electroporation (EP) is used for uptake of chemicals, drugs, DNA into the eukaryotic cells under low electric field (100’s V/cm) of millisecond duration to few hundreds of microsecond duration. An electric field of nanosecond duration and a very high electric field (50’s kV/cm) can stress to intracellular organelles of eukaryotic cells and that can trigger apoptotic pathways. In this article experimental setup has been prepared for real-time investigation of the effect of nanosecond electric field on eukaryotic cells. Pulsar producing ~1.5kV, 20ns FWHM, single shot as well as ~10Hz rep-rate with rise-time as fast as ~10ns, has been prepared using blumlein pulse forming line and avalanche transistor switches. A 300µm gap microplate chamber has been prepared to expose the electric fieldtoeukaryotic cells.

Single-shot real-time electric-field reconstruction of a swept source laser

2015 ◽  
Author(s):  
Thomas Butler ◽  
Svetlana Slepneva ◽  
Ben O'Shaughnessy ◽  
David Goulding ◽  
Bryan Kelleher ◽  
...  
Keyword(s):  
Electric Field ◽  
Real Time ◽  
Single Shot ◽  
Swept Source ◽  
Field Reconstruction

scholarly journals Universal Stokes’s nanomechanical viscometer

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Komal Chaudhary ◽  
Pooja Munjal ◽  
Kamal P. Singh
Keyword(s):  
Electric Field ◽  
Real Time ◽  
Sample Volume ◽  
Capillary Waves ◽  
Small Sample ◽  
Medical Applications ◽  
Rapid Measurement ◽  
Single Lens ◽  
Universal Applicability ◽  
Small Sample Volume

AbstractAlthough, many conventional approaches have been used to measure viscosity of fluids, most methods do not allow non-contact, rapid measurements on small sample volume and have universal applicability to all fluids. Here, we demonstrate a simple yet universal viscometer, as proposed by Stokes more than a century ago, exploiting damping of capillary waves generated electrically and probed optically with sub-nanoscale precision. Using a low electric field local actuation of fluids we generate quasi-monochromatic propagating capillary waves and employ a pair of single-lens based compact interferometers to measure attenuation of capillary waves in real-time. Our setup allows rapid measurement of viscosity of a wide variety of polar, non-polar, transparent, opaque, thin or thick fluids having viscosity values varying over four orders of magnitude from $$10^{0}{-}10^{4}~\text{mPa} \, \text{s}$$ 10 0 - 10 4 mPa s . Furthermore, we discuss two additional damping mechanisms for nanomechanical capillary waves caused by bottom friction and top nano-layer appearing in micro-litre droplets. Such self-stabilized droplets when coupled with precision interferometers form interesting microscopic platform for picomechanical optofluidics for fundamental, industrial and medical applications.

scholarly journals Mitochondrial Dynamics, ROS, and Cell Signaling: A Blended Overview

Life ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 332
Author(s):  
Valentina Brillo ◽  
Leonardo Chieregato ◽  
Luigi Leanza ◽  
Silvia Muccioli ◽  
Roberto Costa
Keyword(s):  
Molecular Mechanisms ◽  
Mitochondrial Dynamics ◽  
Eukaryotic Cells ◽  
Therapeutic Approaches ◽  
Cellular Functions ◽  
Lipid Trafficking ◽  
Mitochondrial Ros ◽  
Intracellular Organelles ◽  
Proliferation Regulation ◽  
Dynamic Plasticity

Mitochondria are key intracellular organelles involved not only in the metabolic state of the cell, but also in several cellular functions, such as proliferation, Calcium signaling, and lipid trafficking. Indeed, these organelles are characterized by continuous events of fission and fusion which contribute to the dynamic plasticity of their network, also strongly influenced by mitochondrial contacts with other subcellular organelles. Nevertheless, mitochondria release a major amount of reactive oxygen species (ROS) inside eukaryotic cells, which are reported to mediate a plethora of both physiological and pathological cellular functions, such as growth and proliferation, regulation of autophagy, apoptosis, and metastasis. Therefore, targeting mitochondrial ROS could be a promising strategy to overcome and hinder the development of diseases such as cancer, where malignant cells, possessing a higher amount of ROS with respect to healthy ones, could be specifically targeted by therapeutic treatments. In this review, we collected the ultimate findings on the blended interplay among mitochondrial shaping, mitochondrial ROS, and several signaling pathways, in order to contribute to the dissection of intracellular molecular mechanisms involved in the pathophysiology of eukaryotic cells, possibly improving future therapeutic approaches.

scholarly journals Graphene Electric Field Sensor Enables Single Shot Label-Free Imaging of Bioelectric Potentials

Nano Letters ◽  
2021 ◽  
Author(s):  
Halleh B. Balch ◽  
Allister F. McGuire ◽  
Jason Horng ◽  
Hsin-Zon Tsai ◽  
Kevin K. Qi ◽  
...  
Keyword(s):  
Electric Field ◽  
Single Shot ◽  
Label Free ◽  
Electric Field Sensor ◽  
Field Sensor ◽  
Bioelectric Potentials

Investigation on dynamic holographic display in liquid crystal film doped with carbon dots

2020 ◽  
Vol 10 (6) ◽  
pp. 780-787
Author(s):  
Hongyue Gao ◽  
Suna Li ◽  
Jicheng Liu ◽  
Wen Zhou ◽  
Fan Xu ◽  
...  
Keyword(s):  
Liquid Crystal ◽  
Electric Field ◽  
Real Time ◽  
Diffraction Efficiency ◽  
Carbon Dots ◽  
Applied Electric Field ◽  
Light Polarization ◽  
Forming Process ◽  
Time Dynamic ◽  
Holographic Display

In this paper, we studied the holographic properties of liquid crystal (LC) thin film doped with carbon dots (CDs) which can be used as real-time holographic display screen. The maximum value of diffraction efficiency can reach up to 30% by using a low applied electric field 0.2 V/μm. Holograms in the LC film can be dynamically formed and self-erased. The hologram build-up time and the hologram self-erasure time in the material is fast enough to realize video refresh rate. In addition, the forming process of hologram was studied. The holographic diffraction efficiency was measured depending on the intensity of recording light, applied electric field, the intensity of readout light, and readout light polarization direction. Triple enhancement of the diffraction efficiency value by the modulation of voltage under the condition of low recording energy is presented. Therefore, we develop an easy way to obtain real-time dynamic holographic red, green and blue displays with high diffraction efficiency, which allow the LC film doped with CDs to be used as a holographic 3D display screen.

scholarly journals Real-time and single shot CMR increases throughput and improves reliability

2012 ◽  
Vol 14 (S1) ◽  
Author(s):  
Abdul Wattar ◽  
Subha V Raman ◽  
Orlando P Simonetti
Keyword(s):  
Real Time ◽  
Single Shot

scholarly journals Real-time switching dynamics of ferroelectric tunnel junctions under single-shot voltage pulses

2018 ◽  
Vol 113 (23) ◽  
pp. 232902 ◽  
Author(s):  
S. Boyn ◽  
A. Chanthbouala ◽  
S. Girod ◽  
C. Carrétéro ◽  
A. Barthélémy ◽  
...  
Keyword(s):  
Real Time ◽  
Tunnel Junctions ◽  
Single Shot ◽  
Time Switching ◽  
Switching Dynamics ◽  
Voltage Pulses
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