scholarly journals Study of RBC Histograms and Its Correlation with Etiopathogenesis and Other Parameters in Various Anemias

2021 ◽  
pp. 66-82
Author(s):  
A. P. Gokula Kannan ◽  
R. Govindarajan ◽  
J. Thanka
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Pluripotent Stem Cells ◽  
Morphological Features ◽  
Peripheral Smear ◽  
Smear Examination ◽  
Cytoplasmic Rna ◽  
Different Types

The pluripotent stem cells which are present in the bone marrow renew by its own and differentiate into mature cells. These stem cells undergo division by the presence of erythropoietin, where the nucleus is extruded out from the cell during the end of differentiation, thereby retaining cytoplasmic RNA to form a reticulocyte. The reticulocyte i s a precursor to red blood cell and on losing the RNA it matures into a Red Blood Cell. The present study aimed to analyze the correlation between the automated histogram patterns along with morphological features of RBC‘s prepared from peripheral smear examination in different types of anemia. viz., MCV, MCH, MCHC & RWD- CV.

Red blood cell generation from human induced pluripotent stem cells of different donor cell type origin

2013 ◽  
Vol 41 (8) ◽  
pp. S27
Author(s):  
Isabel Dorn ◽  
Katharina Klich ◽  
Martina Radstaak ◽  
Katherina Psathaki ◽  
Marcos Arauzo-Bravo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Blood Cell ◽  
Induced Pluripotent Stem Cells ◽  
Red Blood Cell ◽  
Pluripotent Stem Cells ◽  
Donor Cell ◽  
Cell Generation ◽  
Cell Type ◽  
Induced Pluripotent ◽  
Donor Cell Type

Superior Red Blood Cell Generation from Human Pluripotent Stem Cells Through a Novel Microcarrier-Based Embryoid Body Platform

2016 ◽  
Vol 22 (8) ◽  
pp. 765-780 ◽  
Author(s):  
Jaichandran Sivalingam ◽  
Alan Tin-Lun Lam ◽  
Hong Yu Chen ◽  
Bin Xia Yang ◽  
Allen Kuan-Liang Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Pluripotent Stem Cells ◽  
Embryoid Body ◽  
Human Pluripotent Stem Cells ◽  
Cell Generation

scholarly journals CORRELATION OF PERIPHERAL SMEARS FINDINGS WITH RBC INDICES AND RBC HISTOGRAM IN DIAGNOSIS OF ANAEMIA IN PREGNANT FEMALES.

2020 ◽  
pp. 7-9
Author(s):  
Preethi. M ◽  
Ashraf A.Z ◽  
A.S. Anand ◽  
Yaranal P.J
Keyword(s):  
Blood Cell ◽  
Pregnant Women ◽  
Red Blood Cell ◽  
Morphological Changes ◽  
Venous Blood ◽  
Peripheral Blood Smear ◽  
Peripheral Smear ◽  
Smear Examination ◽  
Cell Counts ◽  
Rbc Indices

Background: Red Blood Cell (RBC) histogram is a graphic representation of particle size distribution (cell frequencies verses size). RBC histogram with complete blood cell counts by automated analyser provides an idea about morphological changes of red blood cells in anaemias. Peripheral smear examination (PBS) helps in diagnosing different types of anaemias by visualizing abnormal shape and size, arrangement and immature red cells. This study was intended to analyze various types of anemia in pregnant women and to correlate RBC indices, RBC histogram with peripheral smear. Aims and Objectives: 1) To Correlate RBC indices, RBC histogram with peripheral smear in anemic pregnant women. 2) To categorize the type of anemia based on RBC indices, histogram and peripheral smear. Materials and Methods: Venous blood sample was run in SYSMEX XNL/350 six-part analyser. The haematological investigations of CBC, red blood cell indices (MCV, MCH, MCHC) and RBC histogram were obtained by analyser. Peripheral blood smear study was performed. Results: The present study included 55 cases among which in decreasing order of frequency, NNA (50.9%) was more common followed by MHA (40%), macrocytic anaemia (3.63%) and dimorphic anaemia (5.45%), as categorized by peripheral smear examination. Analysis by erythrocyte indices and histogram showed similar findings except in normocytic, microcytic and dimorphic which were 13.63%, 32.14% and 1.8% respectively. The correlation was done between the diagnosis made by the two methods which showed 76% concordance and discordance of 23.63%. Conclusion: Findings of the present study shows automated analyzer correlated well with PBS in cases of macrocytic and dimorphic anemia, compared to normocytic normochromic and microcytic hypochromic anaemia. As, RBC histogram with RBC indices and PBS are supplementary to each other, both should be used in conjunction for accurate diagnosis.

scholarly journals Red blood cell generation from human induced pluripotent stem cells: perspectives for transfusion medicine

Haematologica ◽  
2010 ◽  
Vol 95 (10) ◽  
pp. 1651-1659 ◽  
Author(s):  
H. Lapillonne ◽  
L. Kobari ◽  
C. Mazurier ◽  
P. Tropel ◽  
M.-C. Giarratana ◽  
...  
Keyword(s):  
Stem Cells ◽  
Blood Cell ◽  
Induced Pluripotent Stem Cells ◽  
Red Blood Cell ◽  
Pluripotent Stem Cells ◽  
Cell Generation ◽  
Transfusion Medicine ◽  
Induced Pluripotent

scholarly journals PS1235 RED BLOOD CELL GENERATION FROM HUMAN INDUCED PLURIPOTENT STEM CELLS

HemaSphere ◽  
2019 ◽  
Vol 3 (S1) ◽  
pp. 562
Author(s):  
E. Varga ◽  
M. Hansen ◽  
S. Heshusius ◽  
P. Burger ◽  
T. Wust ◽  
...  
Keyword(s):  
Stem Cells ◽  
Blood Cell ◽  
Induced Pluripotent Stem Cells ◽  
Red Blood Cell ◽  
Pluripotent Stem Cells ◽  
Cell Generation ◽  
Induced Pluripotent

Differentiation of Human Pluripotent Stem Cells for Red Blood Cell Production

2018 ◽  
pp. 47-62
Author(s):  
Mark C. Allenby ◽  
Susana Brito dos Santos ◽  
Nicki Panoskaltsis ◽  
Athanasios Mantalaris
Keyword(s):  
Stem Cells ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Cell Production

Study of peripheral blood smear findings in patients of anemia and to compare it with automated hematology analyzer generated red cell parameters

2020 ◽  
Vol 5 (3) ◽  
pp. 1231-1235
Author(s):  
Mrinalini Singh ◽  
Santosh Upadhyay Kafle ◽  
Shilpi Shaukin ◽  
Suman Pokhrel
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Macrocytic Anemia ◽  
Hypochromic Anemia ◽  
Peripheral Blood Smear ◽  
Mean Corpuscular Hemoglobin ◽  
Peripheral Smear ◽  
Cell Parameters ◽  
Smear Examination ◽  
Automated Hematology Analyzer

Introduction: Anemia is one of the common medical conditions prevalent in our society. The correct categorization of varying types of anemia is essential for therapeutic purposes. Various laboratory tests are done to find out its underlying cause, but peripheral smear (PS) study of red blood cells (RBCs) morphology is important along with study of red blood cell indices in the classification of anemia. Objectives: This study was done to evaluate RBC morphology on peripheral smear examination in patients of anemia and to compare these findings with cell counter generated red blood cells indices comprising of Mean corpuscular volume (MCV), Mean corpuscular hemoglobin concentration (MCHC) and Mean corpuscular hemoglobin (MCH) Methodology: Red blood cell morphology on peripheral blood smear was studied in 350 patients of anemia irrespective of age and gender received under six months duration  from 1st January 2019 to 30 th June 2019 and  findings  were compared with  cell counter generated  red cell parameters generated by automated  hematology analyzer. Results: Most common anemia seen on smear examination was microcytic hypochromic anemia which accounted for 210 (60%) cases followed by dimorphic anemia in 73 (20.86%) cases. Most of the patients were female, comprising of 227 (64.85%) cases. Highest numbers of patient were in the age group of 21-30 years (20.30%). Sensitivity of MCV was 78%, MCHC was 14% and MCH was 80% in detection of microcytic hypochromic anemia. The sensitivity of MCV and MCH was found to be 100 %  in detection of macrocytic anemia. Sensitivity of MCHC was only 10% for detection of macrocytic anemia. The sensitivity of MCV, MCHC and MCH was 78%, 100% and 67 % respectively in detection of normocytic normochromic anemia.  Conclusion: The peripheral smear examination should always be interpreted along with the red blood cell indices generated by an automated analyzer in order to classify various types of anemia.

RBC Histogram Predictive of HE.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 3706-3706
Author(s):  
Carl Chakmakjian ◽  
Ed Rappaport
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Peripheral Smear ◽  
Consistent Finding ◽  
Hereditary Elliptocytosis ◽  
Smear Examination ◽  
Chronic Hemolysis

Abstract Hereditary elliptocytosis (HE) encompasses a family of inherited erythrocyte disorders characterized by red blood cells with an oval, elongated shape. Diagnosis is most commonly made upon peripheral smear examination. Although the disorder is usually clinically silent, these patients may have some degree of chronic hemolysis that may necessitate splenectomy on occasion. We report a consistent finding in HE common to most electronic cell counters. The red blood cell histogram reliably has a two to three millimeter elevation above baseline at the extreme left of the graph. This “foot” on the histogram is predictive of possible red blood cell elliptocytes. Please see example. Figure Figure

Superior Red Blood Cell Generation From Human Pluripotent Stem Cells via a Novel Microcarrier Based Embryoid Body Platform

Cytotherapy ◽  
2016 ◽  
Vol 18 (6) ◽  
pp. S41-S42
Author(s):  
J. Sivalingam ◽  
E. Lee ◽  
A. Lam ◽  
A. Chen ◽  
S. Reuveny ◽  
...  
Keyword(s):  
Stem Cells ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Pluripotent Stem Cells ◽  
Embryoid Body ◽  
Human Pluripotent Stem Cells ◽  
Cell Generation
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