scholarly journals CORRELATION OF PERIPHERAL SMEARS FINDINGS WITH RBC INDICES AND RBC HISTOGRAM IN DIAGNOSIS OF ANAEMIA IN PREGNANT FEMALES.

2020 ◽  
pp. 7-9
Author(s):  
Preethi. M ◽  
Ashraf A.Z ◽  
A.S. Anand ◽  
Yaranal P.J
Keyword(s):  
Blood Cell ◽  
Pregnant Women ◽  
Red Blood Cell ◽  
Morphological Changes ◽  
Venous Blood ◽  
Peripheral Blood Smear ◽  
Peripheral Smear ◽  
Smear Examination ◽  
Cell Counts ◽  
Rbc Indices

Background: Red Blood Cell (RBC) histogram is a graphic representation of particle size distribution (cell frequencies verses size). RBC histogram with complete blood cell counts by automated analyser provides an idea about morphological changes of red blood cells in anaemias. Peripheral smear examination (PBS) helps in diagnosing different types of anaemias by visualizing abnormal shape and size, arrangement and immature red cells. This study was intended to analyze various types of anemia in pregnant women and to correlate RBC indices, RBC histogram with peripheral smear. Aims and Objectives: 1) To Correlate RBC indices, RBC histogram with peripheral smear in anemic pregnant women. 2) To categorize the type of anemia based on RBC indices, histogram and peripheral smear. Materials and Methods: Venous blood sample was run in SYSMEX XNL/350 six-part analyser. The haematological investigations of CBC, red blood cell indices (MCV, MCH, MCHC) and RBC histogram were obtained by analyser. Peripheral blood smear study was performed. Results: The present study included 55 cases among which in decreasing order of frequency, NNA (50.9%) was more common followed by MHA (40%), macrocytic anaemia (3.63%) and dimorphic anaemia (5.45%), as categorized by peripheral smear examination. Analysis by erythrocyte indices and histogram showed similar findings except in normocytic, microcytic and dimorphic which were 13.63%, 32.14% and 1.8% respectively. The correlation was done between the diagnosis made by the two methods which showed 76% concordance and discordance of 23.63%. Conclusion: Findings of the present study shows automated analyzer correlated well with PBS in cases of macrocytic and dimorphic anemia, compared to normocytic normochromic and microcytic hypochromic anaemia. As, RBC histogram with RBC indices and PBS are supplementary to each other, both should be used in conjunction for accurate diagnosis.

Study of peripheral blood smear findings in patients of anemia and to compare it with automated hematology analyzer generated red cell parameters

2020 ◽  
Vol 5 (3) ◽  
pp. 1231-1235
Author(s):  
Mrinalini Singh ◽  
Santosh Upadhyay Kafle ◽  
Shilpi Shaukin ◽  
Suman Pokhrel
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Macrocytic Anemia ◽  
Hypochromic Anemia ◽  
Peripheral Blood Smear ◽  
Mean Corpuscular Hemoglobin ◽  
Peripheral Smear ◽  
Cell Parameters ◽  
Smear Examination ◽  
Automated Hematology Analyzer

Introduction: Anemia is one of the common medical conditions prevalent in our society. The correct categorization of varying types of anemia is essential for therapeutic purposes. Various laboratory tests are done to find out its underlying cause, but peripheral smear (PS) study of red blood cells (RBCs) morphology is important along with study of red blood cell indices in the classification of anemia. Objectives: This study was done to evaluate RBC morphology on peripheral smear examination in patients of anemia and to compare these findings with cell counter generated red blood cells indices comprising of Mean corpuscular volume (MCV), Mean corpuscular hemoglobin concentration (MCHC) and Mean corpuscular hemoglobin (MCH) Methodology: Red blood cell morphology on peripheral blood smear was studied in 350 patients of anemia irrespective of age and gender received under six months duration  from 1st January 2019 to 30 th June 2019 and  findings  were compared with  cell counter generated  red cell parameters generated by automated  hematology analyzer. Results: Most common anemia seen on smear examination was microcytic hypochromic anemia which accounted for 210 (60%) cases followed by dimorphic anemia in 73 (20.86%) cases. Most of the patients were female, comprising of 227 (64.85%) cases. Highest numbers of patient were in the age group of 21-30 years (20.30%). Sensitivity of MCV was 78%, MCHC was 14% and MCH was 80% in detection of microcytic hypochromic anemia. The sensitivity of MCV and MCH was found to be 100 %  in detection of macrocytic anemia. Sensitivity of MCHC was only 10% for detection of macrocytic anemia. The sensitivity of MCV, MCHC and MCH was 78%, 100% and 67 % respectively in detection of normocytic normochromic anemia.  Conclusion: The peripheral smear examination should always be interpreted along with the red blood cell indices generated by an automated analyzer in order to classify various types of anemia.

scholarly journals Study of RBC Histograms and Its Correlation with Etiopathogenesis and Other Parameters in Various Anemias

2021 ◽  
pp. 66-82
Author(s):  
A. P. Gokula Kannan ◽  
R. Govindarajan ◽  
J. Thanka
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Pluripotent Stem Cells ◽  
Morphological Features ◽  
Peripheral Smear ◽  
Smear Examination ◽  
Cytoplasmic Rna ◽  
Different Types

The pluripotent stem cells which are present in the bone marrow renew by its own and differentiate into mature cells. These stem cells undergo division by the presence of erythropoietin, where the nucleus is extruded out from the cell during the end of differentiation, thereby retaining cytoplasmic RNA to form a reticulocyte. The reticulocyte i s a precursor to red blood cell and on losing the RNA it matures into a Red Blood Cell. The present study aimed to analyze the correlation between the automated histogram patterns along with morphological features of RBC‘s prepared from peripheral smear examination in different types of anemia. viz., MCV, MCH, MCHC & RWD- CV.

scholarly journals THE HEMOGLOBIN OF HEALTHY COLLEGE UNDERGRADUATES AND COMPARISONS WITH VARIOUS MEDICAL, SOCIAL, PHYSIOLOGIC AND OTHER FACTORS

Blood ◽  
1948 ◽  
Vol 3 (5) ◽  
pp. 566-572 ◽  
Author(s):  
CLARK W. HEATH
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Significant Relationship ◽  
Venous Blood ◽  
College Men ◽  
Hemoglobin Level ◽  
Emotional Factors ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Capacity Method

Abstract 1. The distribution of hemoglobins (obtained by the Sahli method on venous blood and by the O2 capacity method of Van Slyke) and the red blood cell counts of 153 to 259 college men, selected for participation in the Grant Study, are reported. 2. There is a statistically significant relationship between the hemoglobin and the pulse rate (those subjects having higher pulse rates tending to have higher hemoglobins). This relationship is apparently associated with emotional factors. 3. No significant relationship could be established between hemoglobin level and a variety of other factors, including socio-economic, dietary, physiologic, medical, body-build and others.

scholarly journals Glycated hemoglobin and red blood cell indices in non-diabetic pregnant women

2017 ◽  
Vol 7 (4) ◽  
Author(s):  
Awad-Elkareem Abass ◽  
Imad R. Musa ◽  
Duria A. Rayis ◽  
Ishag Adam ◽  
Gasim I. Gasim
Keyword(s):  
Body Mass Index ◽  
Blood Cell ◽  
Pregnant Women ◽  
Body Mass ◽  
Red Blood Cell ◽  
Significant Positive Correlation ◽  
Complete Blood Count ◽  
Linear Regression Analysis ◽  
Positive Correlation ◽  
Rbc Indices

Hemoglobin A1c (HbA1c) is affected by the red blood cell (RBC) lifespan. The association of HbA1c with RBC indices in pregnant women is not widely investigated. In this study, we investigated the association between HbA1c and Hb/RBCs count and RBCs indices; specifically, Hb/RBCs count and indices in non- diabetic pregnant women. Across-sectional study was carried- out at the antenatal care of Saad Abu Elela Hospital, Khartoum, Sudan. Obstetrics history was gathered using questionnaire and body mass index was calculated. Fasting blood sugar, at one hour, at two hours post prandial and HbA1c were investigated. Complete blood count parameters of RBCs count was performed, including, hematocrit, Hb, RBCs indices of MCV, MCH, and MCHC. One hundred twenty three women were enrolled. The mean age of the participants was 28±5.6 years and a mean body mass index was 27.65±6.8 k/m<sup>2</sup>. There was significant positive correlation between HbA1c and Hb (r=0.174, P=0.037), Hct (r=0.174, P=0.037), and MCHC (r=0.180, P=0.031). A negative correlation between HbA1c and the platelet index PDW (r= –0.198, P=0.017) was documented. The rest of the other factors showed no correlation with HbA1c. In linear regression analysis, HbA1c correlated positively with Hb (P=0.044) and Hct (P=0.047). The present study shows a significant positive correlation between HbA1c value with Hb, Hct, and MCHC. No significant correlation between HbA1c and other RBCs parameters was observed.

RBC Histogram Predictive of HE.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 3706-3706
Author(s):  
Carl Chakmakjian ◽  
Ed Rappaport
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Peripheral Smear ◽  
Consistent Finding ◽  
Hereditary Elliptocytosis ◽  
Smear Examination ◽  
Chronic Hemolysis

Abstract Hereditary elliptocytosis (HE) encompasses a family of inherited erythrocyte disorders characterized by red blood cells with an oval, elongated shape. Diagnosis is most commonly made upon peripheral smear examination. Although the disorder is usually clinically silent, these patients may have some degree of chronic hemolysis that may necessitate splenectomy on occasion. We report a consistent finding in HE common to most electronic cell counters. The red blood cell histogram reliably has a two to three millimeter elevation above baseline at the extreme left of the graph. This “foot” on the histogram is predictive of possible red blood cell elliptocytes. Please see example. Figure Figure

scholarly journals Changes of Hematocrit, Hemoglobin, Serum Protein Levels and Red Blood Cell Counts Before and After Stroke

1976 ◽  
Vol 13 (4) ◽  
pp. 207-214
Author(s):  
Hiroshi Yamanouchi ◽  
Hideo Tohgi ◽  
Masakuni Kameyama ◽  
Mototaka Murakami ◽  
Tamotsu Matsuda
Keyword(s):  
Blood Cell ◽  
Serum Protein ◽  
Red Blood Cell ◽  
Protein Levels ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Before And After

scholarly journals The Sensitivity and Specificity of a Red Blood Cell Agglutination D-Dimer Assay for Venous Thromboembolism When Performed on Venous Blood

2002 ◽  
Vol 162 (2) ◽  
pp. 217 ◽  
Author(s):  
Sanjeev D. Chunilal ◽  
Patrick A. Brill-Edwards ◽  
Pamela B. Stevens ◽  
Jody P. Joval ◽  
Joanne A. McGinnis ◽  
...  
Keyword(s):  
Venous Thromboembolism ◽  
Blood Cell ◽  
Sensitivity And Specificity ◽  
Red Blood Cell ◽  
Venous Blood ◽  
Cell Agglutination ◽  
D Dimer

scholarly journals Changes in the Gut Microbiome of the Sea Lamprey during Metamorphosis

2012 ◽  
Vol 78 (21) ◽  
pp. 7638-7644 ◽  
Author(s):  
Amanda Tetlock ◽  
Christopher K. Yost ◽  
John Stavrinides ◽  
Richard G. Manzon
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Gut Microbiome ◽  
Morphological Changes ◽  
Sea Lamprey ◽  
Phenotypic Characterization ◽  
Filter Feeding ◽  
Content Type ◽  
Organ Systems ◽  
Feeding Larva

ABSTRACTVertebrate metamorphosis is often marked by dramatic morphological and physiological changes of the alimentary tract, along with major shifts in diet following development from larva to adult. Little is known about how these developmental changes impact the gut microbiome of the host organism. The metamorphosis of the sea lamprey (Petromyzon marinus) from a sedentary filter-feeding larva to a free-swimming sanguivorous parasite is characterized by major physiological and morphological changes to all organ systems. The transformation of the alimentary canal includes closure of the larval esophagus and the physical isolation of the pharynx from the remainder of the gut, which results in a nonfeeding period that can last up to 8 months. To determine how the gut microbiome is affected by metamorphosis, the microbial communities of feeding and nonfeeding larval and parasitic sea lamprey were surveyed using both culture-dependent and -independent methods. Our results show that the gut of the filter-feeding larva contains a greater diversity of bacteria than that of the blood-feeding parasite, with the parasite gut being dominated byAeromonasand, to a lesser extent,CitrobacterandShewanella. Phylogenetic analysis of the culturableAeromonasfrom both the larval and parasitic gut revealed that at least five distinct species were represented. Phenotypic characterization of these isolates revealed that over half were capable of sheep red blood cell hemolysis, but all were capable of trout red blood cell hemolysis. This suggests that the enrichment ofAeromonasthat accompanies metamorphosis is likely related to the sanguivorous lifestyle of the parasitic sea lamprey.

Kynurenic Acid in Synovial Fluid and Serum of Patients with Rheumatoid Arthritis, Spondyloarthropathy, and Osteoarthritis

2013 ◽  
Vol 40 (6) ◽  
pp. 903-909 ◽  
Author(s):  
Jolanta Parada-Turska ◽  
Wojciech Zgrajka ◽  
Maria Majdan
Keyword(s):  
Rheumatoid Arthritis ◽  
Regression Analysis ◽  
Synovial Fluid ◽  
Blood Cell ◽  
Red Blood Cell ◽  
High Performance ◽  
Kynurenic Acid ◽  
Linear Regression Analysis ◽  
Cell Counts

Objective.Previously we demonstrated that kynurenic acid (KYNA), an endogenous metabolite of kynurenine, is present in the synovial fluid of patients with rheumatoid arthritis (RA). KYNA inhibits proliferation of synoviocytesin vitro. The goal of our study was to compare KYNA concentrations in synovial fluid and blood of patients with RA, inflammatory spondyloarthropathies (SpA), and osteoarthritis (OA).Methods.Serum and synovial fluid samples were obtained from 189 patients with RA, 56 patients with SpA, and 32 patients with OA. KYNA was separated using a high-performance liquid chromatography system and measured fluorometrically.Results.KYNA concentration in synovial fluid obtained from patients with RA and SpA was significantly lower than that in patients with OA (p < 0.05). The concentration of KYNA in serum of patients with RA, SpA, and OA did not differ among all groups studied. The positive correlation between KYNA content in synovial fluid and serum was found in patients with RA (p < 0.05). Univariate linear regression analysis demonstrated that fibrinogen was significantly associated with KYNA in synovial fluid (p < 0.05), and red blood cell counts, morning stiffness, and pain scores were significantly associated with KYNA level in serum (all p < 0.05). Multivariate regression analysis revealed correlation between the following independent variables: hemoglobin level, hematocrit, red blood cell count in conjunction with age and KYNA content in synovial fluid. A lack of correlation was observed between KYNA content in synovial fluid of patients with RA and other clinical and laboratory measures of disease activity.Conclusion.Our data show a local deficit of KYNA in inflammatory states.

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