scholarly journals Detection and Assay of Riboflavin (Vitamin B2) Utilizing UV/VIS Spectrophotometer and Citric Acid Buffer

2014 ◽  
Vol 3 (6) ◽  
pp. 799-809
Author(s):  
Ronald Bartzatt
Keyword(s):  
Citric Acid ◽  
Vitamin B2 ◽  
Acid Buffer ◽  
Citric Acid Buffer

scholarly journals Oligosaccharide-based Surfactant/Citric Acid Buffer System Stabilizes Lactate Dehydrogenase during Freeze-drying and Storage without the Addition of Natural Sugar

2016 ◽  
Vol 65 (6) ◽  
pp. 525-532 ◽  
Author(s):  
Shigesaburo Ogawa ◽  
Ryuichiro Kawai ◽  
Maito Koga ◽  
Kouichi Asakura ◽  
Isao Takahashi ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Citric Acid ◽  
Freeze Drying ◽  
Buffer System ◽  
Acid Buffer ◽  
Citric Acid Buffer ◽  
Acid Buffer System ◽  
And Storage

scholarly journals The bivalent-cation dependence of phosphatidylinositol synthesis in a cell-free system from lymphocytes

1983 ◽  
Vol 212 (3) ◽  
pp. 691-697 ◽  
Author(s):  
J P Moore ◽  
G A Smith ◽  
T R Hesketh ◽  
J C Metcalfe
Keyword(s):  
Citric Acid ◽  
Phosphatidic Acid ◽  
Bivalent Cation ◽  
Free System ◽  
Cell Free System ◽  
Acid Buffer ◽  
Physiological Conditions ◽  
Citric Acid Buffer ◽  
A Cell

The bivalent-cation requirements of two enzymes involved in phosphatidylinositol synthesis were defined for pig lymphocyte membranes using a citric acid buffer. CTP:phosphatidic acid cytidylyltransferase (EC 2.7.7.41) is activated by free Mn2+ concentrations above 20nM and by free Mg2+ concentrations above 10 microM. When activated by Mg2+, the enzyme is weakly inhibited by Ca2+ (Ki greater than 250 microM), but Ca2+ has no effect when Mn2+ is used to stimulate CDP-diacylglycerol synthesis. The synthesis of phosphatidylinositol from phosphatidic acid is also stimulated by Mn2+ and Mg2+ concentrations similar to those above and is inhibited by free Ca2+ concentrations above 500nM, probably by its action on CDP-diacylglycerol:inositol 3-phosphatidyltransferase (EC 2.7.8.11). Taken together, these studies suggest that under physiological conditions phosphatidylinositol synthesis is activated by Mg2+ and it is possible that it is further regulated by the free concentrations of Ca2+ and/or Mn2+.

Leukocyte β-Galactosidase Activity in GM1-Gangliosidosis

PEDIATRICS ◽  
1972 ◽  
Vol 50 (3) ◽  
pp. 502-503
Author(s):  
Elisabeth Young ◽  
R. B. Ellis ◽  
A. D. Patrick
Keyword(s):  
Citric Acid ◽  
Recent Article ◽  
Galactosidase Activity ◽  
Alternative Substrate ◽  
Gm1 Gangliosidosis ◽  
Acid Buffer ◽  
Assay Medium ◽  
Routine Service ◽  
Citric Acid Buffer ◽  
Beta Galactosidase

The recent article by Singer et al. entitled "Leukocyte beta-Galactosidase Activity in the Diagnosis of Generalized GM1-Gangliosidosis," prompts us to report the results of two years of routine service assay of this enzyme in leukocytes using the alternative substrate, 4-methyl-umbelliferyl β-D-galactopyranoside. The β-galactosidase assay medium contained McIlvaine phosphate-citric acid buffer, pH, 4.1 (40µl); 4-methylumbelliferyl β-D-galactopyranoside (1 mM in buffer, 150µl) and leukocyte extract in 0.2 MKCl (10µl). After incubation for 15 minutes at 30°C, the reaction was stopped by the addition of 0.25 M glycine-NaOH, pH, 10.4 (1 ml) and the fluorescence measured.

An Immunonanosilver Resonance Rayleigh Scattering Spectral Probe for Rapid Assay of Human Chorionic Gonadotrophin

2013 ◽  
Vol 680 ◽  
pp. 141-144 ◽  
Author(s):  
Qing Ye Liu ◽  
Gui Qing Wen ◽  
Kun Li ◽  
Ai Hui Liang
Keyword(s):  
Citric Acid ◽  
Optimal Condition ◽  
Rayleigh Scattering ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Resonance Rayleigh Scattering ◽  
Serum Samples ◽  
Buffer Solution ◽  
Citric Acid Buffer

In pH 6.6 Na2HPO4- citric acid buffer solution and in the presence of KCl, the immunoreaction between hCG and nanosilver-labeled anti-hCG took place, the immunonanosilver-complex was formed and deposited, caused the resonance Rayleigh scattering (RRS) intensity at 510 nm decreased. In the optimal condition, the decreased RRS intensity responds linearly with the concentration of hCG over 0.125-1.75 µg/mL. Based on this, a new and simple RRS method has been proposed for the determination of hCG in serum samples, with satisfactory results.

scholarly journals Bi/mZVI Combined with Citric Acid and Sodium Citrate to Mineralize Multiple Sulfa Antibiotics: Performance and Mechanism

Antibiotics ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 51
Author(s):  
Xiaoming Su ◽  
Hao Lv ◽  
Jianyu Gong ◽  
Man Zhou
Keyword(s):  
Citric Acid ◽  
Sodium Citrate ◽  
Degradation Pathway ◽  
Degradation Pathways ◽  
Buffer Solution ◽  
Efficient Treatment ◽  
Theoretical Support ◽  
Citric Acid Buffer ◽  
Iron Based ◽  
Performance And Mechanism

The oxidative mineralization of sulfanilamide drugs (SAs) using micro-size zero-valent iron (mZVI) cooperated with a citric acid buffer solution was evaluated. In this study SM2, SMX, and SD could be removed at 66%, 89%, and 83%, respectively, in a 0.5% Bi/mZVI+CA+NaCA system within 2 h. Based on our analysis, the produced ·OH could be ascribed from the complexation between citrate iron (Fe(II)[Cit]−) and the generated H2O2 resulting from the activation of O2 on the mZVI surface in the Bi/mZVI+CA+NaCA system, further inducing the mineralization of antibiotics. The related possible degradation pathways were proposed. Two similar degradation pathways of SM2, SMX, and SD in the mixed liquid, including hydroxylation and SO2 extrusion, were solved. Meanwhile, there was an additional proposed degradation pathway for SMX to be degraded more effectively, as reflected in the opening of the N-O bond on the benzene ring. Therefore, this work provides an experimental basis and theoretical support for the efficient treatment of antibiotic wastewater in real industry by using an iron-based method.

Vitamin B2 may help treat sepsis

Nature ◽  
2004 ◽  
Author(s):  
HelenR. Pilcher
Keyword(s):  
Vitamin B2
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