Botanica Pacifica plant chromosome data 1

The chromosome numbers (2n) are presented for 82 vascular plant species of 61 genera from 31 families: Amaranthaceae: Amaranthus, Atriplex; Apiaceae: Angelica, Pimpinella, Torilis; Araceae: Calla; Asteraceae: Bidens, Hieracium, Lactuca, Leontodon, Solidago; Boraginaceae: Cerinthe, Lappula; Brassicaceae: Fibigia; Campanulaceae: Platycodon; Caryophyllaceae: Dianthus, Eremogone; Cupressaceae: Cupressus; Ericaceae: Rhododendron; Euphorbiaceae: Euphorbia; Fabaceae: Acacia, Astragalus, Galega, Lotus, Robinia, Trifolium, Vicia; Grossulariaceae: Ribes; Iridaceae: Iris; Lamiaceae: Leonurus, Lycopus; Liliaceae: Lilium; Nelumbonaceae: Nelumbo; Onagraceae: Chamaenerion; Orobanchaceae: Pedicularis; Paeoniaceae: Paeonia; Papaveraceae: Chelidonium, Glaucium, Papaver; Plantaginaceae: Plantago; Poaceae: Achnatherum, Calamagrostis, Digitaria, Eragrostis, Glyceria, Milium, Phleum, Poa, Puccinellia; Polygonaceae: Persicaria, Rumex; Primulaceae: Anagallis; Ranunculaceae: Delphinium, Pulsatilla; Rosaceae: Potentilla, Sibbaldia, Spiraea; Rubiaceae: Asperula; Urticaceae: Parietaria; Valerianaceae: Valeriana; Violaceae: Viola. The species studied are from East Europe (Ukraine), Siberia (Irkutsk Region, Novosibirsk Region, Republic of Buryatia), Caucasus (Abkhazia, Armenia, Azerbaijan, Georgia, Russia), Middle Asia (Kazakhstan, Kyrgyzstan, Turkmenistan, Uzbekistan) and the Russian Far East (Amur Region, Khabarovsk Territory, Primorye Territory). Most of the species are diploids, with different basic numbers (x). Species with variable ploidy also were revealed. The CN data are accompanied with the brief information on ecology and distribution of the species studied.

INDUCED IN VIVO MUTAGENESIS USING COLCHICINE IN HYDROGEN PEROXIDE ON M2 COWPEA, YARDLONG BEAN AND COMMON BEAN

Research on doubling plant chromosome using colchicine has been carried out for years with water as its solvent. In this study, an innovation was carried out by trying to do colchicine treatment in hydrogen peroxide. In this in vivo induced mutagenesis research, colchicine in hydrogen peroxide was administered as mutagen on seeds three species of crops i.e. mutan M2 cowpea, yardlong bean, and common bean by soaking method. The results of this research showed that three times multistep mutant (M3) of cowpea was having mutant characteristics that the shape of its leaflet changed from rhombus to ovate. Besides, both yardlong bean varieties used in this research (BLR and KTR) showed indication of a mutant crop that having compound leaves with more than three leaflets in a stalk. Common bean of PTW variety was not attacked by any aphid but it was attacked by leafminer. Finally, we found “rainbow common bean” from this research.