scholarly journals Multiple displacement amplification of the DNA from single flow-sorted plant chromosome

2015 ◽  
Vol 84 (4) ◽  
pp. 838-844 ◽  
Author(s):  
Petr Cápal ◽  
Nicolas Blavet ◽  
Jan Vrána ◽  
Marie Kubaláková ◽  
Jaroslav Doležel
Keyword(s):  
Multiple Displacement Amplification ◽  
Plant Chromosome ◽  
Single Flow

scholarly journals Genomic DNA Amplification from a Single Bacterium

2005 ◽  
Vol 71 (6) ◽  
pp. 3342-3347 ◽  
Author(s):  
Arumugham Raghunathan ◽  
Harley R. Ferguson ◽  
Carole J. Bornarth ◽  
Wanmin Song ◽  
Mark Driscoll ◽  
...  
Keyword(s):  
16S Rrna ◽  
Dna Polymerase ◽  
Genomic Dna ◽  
Multiple Displacement Amplification ◽  
Dna Amplification ◽  
Rrna Gene ◽  
Bacterial Cells ◽  
Single Flow ◽  
The 16S Rrna Gene ◽  
New Strategies

ABSTRACT Genomic DNA was amplified about 5 billion-fold from single, flow-sorted bacterial cells by the multiple displacement amplification (MDA) reaction, using φ 29 DNA polymerase. A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies for studying nonculturable microorganisms.

Diagnostic Value of Non-Invasive Prenatal Screening of β-thalassemia by Cell Free Fetal DNA and Fetal NRBC

2019 ◽  
Vol 19 (2) ◽  
pp. 105-111
Author(s):  
Nadia Shafei ◽  
Mohammad Saeed Hakhamaneshi ◽  
Massoud Houshmand ◽  
Siavash Gerayeshnejad ◽  
Fardin Fathi ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Multiple Displacement Amplification ◽  
Diagnostic Value ◽  
Diagnostic Methods ◽  
Beta Thalassemia ◽  
Non Invasive ◽  
Fetal Dna ◽  
Prenatal Diagnostic ◽  
Cell Free Fetal Dna ◽  
Invasive Techniques

Background: Beta thalassemia is a common disorder with autosomal recessive inheritance. The most prenatal diagnostic methods are the invasive techniques that have the risk of miscarriage. Now the non-invasive methods will be gradually alternative for these invasive techniques. Objective: The aim of this study is to evaluate and compare the diagnostic value of two non-invasive diagnostic methods for fetal thalassemia using cell free fetal DNA (cff-DNA) and nucleated RBC (NRBC) in one sampling community. Methods: 10 ml of blood was taken in two k3EDTA tube from 32 pregnant women (mean of gestational age = 11 weeks), who themselves and their husbands had minor thalassemia. One tube was used to enrich NRBC and other was used for cff-DNA extraction. NRBCs were isolated by MACS method and immunohistochemistry; the genome of stained cells was amplified by multiple displacement amplification (MDA) procedure. These products were used as template in b-globin segments PCR. cff-DNA was extracted by THP method and 300 bp areas were recovered from the agarose gel as fetus DNA. These DNA were used as template in touch down PCR to amplify b-globin gen. The amplified b-globin segments were sequenced and the results compared with CVS resul. Results: The data showed that sensitivity and specificity of thalassemia diagnosis by NRBC were 100% and 92% respectively and sensitivity and specificity of thalassemia diagnosis by cff-DNA were 100% and 84% respectively. Conclusion: These methods with high sensitivity can be used as screening test but due to their lower specificity than CVS, they cannot be used as diagnostic test.

scholarly journals Cover Feature: A Single‐Flow Battery with Multiphase Flow (ChemSusChem 4/2021)

ChemSusChem ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 991-991
Author(s):  
Lihi Amit ◽  
Danny Naar ◽  
Robert Gloukhovski ◽  
Gerardo Jose O' ◽  
Matthew E. Suss
Keyword(s):  
Multiphase Flow ◽  
Flow Battery ◽  
Single Flow

scholarly journals Quantifying genome DNA during whole-genome amplification via quantitative real-time multiple displacement amplification

RSC Advances ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 4617-4621
Author(s):  
Jing Tu ◽  
Yi Qiao ◽  
Yuhan Luo ◽  
Naiyun Long ◽  
Zuhong Lu
Keyword(s):  
Real Time ◽  
Whole Genome Amplification ◽  
Multiple Displacement Amplification ◽  
Whole Genome ◽  
Genome Amplification

Monitoring multiple displacement amplification by fluorescence signals.

Tab Design Based on the Internal Distributed Properties in a Zinc–Nickel Single-Flow Battery

2021 ◽  
Vol 60 (3) ◽  
pp. 1434-1451
Author(s):  
Rui Zhou ◽  
Shouguang Yao ◽  
Yunhui Zhao ◽  
Jie Cheng
Keyword(s):  
Flow Battery ◽  
Single Flow ◽  
Zinc Nickel

Single Flow Multiphase Flow Batteries: Theory

2021 ◽  
pp. 138554
Author(s):  
R Ronen ◽  
A.D. Gat ◽  
M.Z. Bazant ◽  
M.E. Suss
Keyword(s):  
Multiphase Flow ◽  
Flow Batteries ◽  
Single Flow

The High Pressure Rheology of Mixtures

2004 ◽  
Vol 126 (4) ◽  
pp. 697-702 ◽  
Author(s):  
Scott Bair
Keyword(s):  
Molecular Weight ◽  
High Pressure ◽  
Binary Systems ◽  
Viscosity Coefficient ◽  
Shear Thinning ◽  
Newtonian Viscosity ◽  
Double Shear ◽  
Ideal Mixing ◽  
Viscosity Behavior ◽  
Single Flow

The Newtonian mixing rules for several binary systems have been experimentally investigated. Some systems show non-ideal mixing response and for some systems the non-ideal response is pressure-dependent, yielding an opportunity for manipulation of the pressure-viscosity behavior to advantage. The mixing of differing molecular weight “straight cuts” can produce very different pressure-viscosity response. This behavior underscores the difficulty in predicting the pressure-viscosity coefficient based upon chemical structure and ambient viscosity since the molecular weight distribution is also important, but it also provides another opportunity to control the high-pressure response by blending. The first experimental observation of double shear-thinning within a single flow curve is reported. Blending then provides the capability of adjusting not only the Newtonian viscosity but also the non-Newtonian shear-thinning response as well.

scholarly journals Development of Microbial Genome-Probing Microarrays using Digital Multiple Displacement Amplification of Uncultivated Microbial Single Cells

2008 ◽  
Vol 42 (22) ◽  
pp. 8614-8614
Author(s):  
Ho-Won Chang ◽  
Youboong Sung ◽  
Kyoung-Ho Kim ◽  
Young-Do Nam ◽  
Seong Woon Roh ◽  
...  
Keyword(s):  
Multiple Displacement Amplification ◽  
Single Cells ◽  
Microbial Genome
Sign in / Sign up

Export Citation Format

Share Document