Soya protein isolate-polyethylene oxide electrospun nanofiber membrane with bone marrow-derived mesenchymal stem cell for enhanced bone regeneration

In this study, we prepared an electrospun nanofiber membrane from soya protein isolate (SPI) and polyethylene oxide (PEO) loaded with rat bone marrow-derived mesenchymal stem cells (rBMSC), as a cell-scaffold approach to enhance bone regeneration. Different ratios of SPI:PEO (7:0, 7:1, 7:3, 7:5, and 0:7) was investigated to obtain uniform nanofibers, and crosslinked with EDC/NHS to stabilize the membranes. SPI/PEO membrane (7:3) was found to create more uniform and stable nanofibers at a flow rate of 9 µL/min, spun in a cylindrical collector rotating at 350 r/min, 23 kV DC voltage, and needle tip to collector distance of 13 cm. The loaded rBMSC were pre-differentiated to ensure commitment towards osteoblastic lineage. The SPI/PEO electrospun nanofiber membranes were successful in allowing for cell attachment and growth of the rBMSC and was further investigated in vivo using a rat skull defect model. New bone formation was observed for the optimized SPI/PEO electrospun nanofiber membrane (7:3) with and without rBMSC, but with faster new bone formation for SPI/PEO electrospun nanofiber membrane loaded with rBMSC as compared to SPI/PEO electrospun nanofiber membrane only and control (defect only).

INVESTIGATION OF FUNCTIONAL-TECHNOLOGICAL PROPERTIES OF SOYA PROTEIN

There was offered and grounded the use of functional technological properties of the soya protein isolate in the technology of oil pasts. It will allows to increase the balance of the oil past composition additionally and will favor the decrease of extracting moisture during the storage term. There was studied the dynamics of a gradient of the limit stress of soya protein: hydrated soya protein, hydrated soya protein with the temperature processing, hydrated soya protein with the preliminary keeping during 24 h, hydrated soya protein with the preliminary keeping during 24 h and temperature processing during 5 min, hydrated soya protein with the preliminary keeping during 24 h and temperature processing during 10 min; hydrated soya protein with the preliminary keeping during 24 h and temperature processing during 15 min. It was established, that the hydrated isolate of soya protein is a plastic system, has enough strength. The limit stress parameter at the variable velocity of deformation of model samples determines optimal technological parameters of preparing the soya isolate: hydromodule – 1: 8, temperature processing – (82±2) °С, process duration 10 min with preliminary keeping during 24 hours. As a result of the studies, there were demonstrated technological parameters of preparing the soya protein isolate for obtaining the oil past by the direct mixing with the oil base.

Sex- and age-specific immunomodulatory effects of dietary soya protein isolate and isoflavones in rats

The present study examined, using rats as a model, the effects of sex and age of exposure to dietary soya components on serum total and soya-specific antibody content. In Expt 1, Sprague–Dawley rats at 50 d of age were fed diets containing 20 % casein or 20 % alcohol-washed soya protein isolate (SPI) with or without supplemental isoflavones (ISF, 250 mg/kg diet) for 70, 190 or 310 d. The offspring were fed the same diets as their parents. In Expt 2, juvenile Sprague–Dawley rats at 30 d of age were fed diets containing 20 % casein with or without supplemental ISF (50 mg/kg diet) or increasing amounts of alcohol-washed SPI (5, 10 or 20 %) for 90 d. Exposure of rats to dietary SPI before the age of 28 d increased serum total IgA and IgM, and induced the production of SPI-specific IgA, IgG, IgM and IgE antibodies. Feeding of juvenile or adult rats with SPI elevated serum total IgA in females, while the opposite occurred in males, and markedly stimulated the production of SPI-specific IgM in females and IgG in males. Our data suggest that the effects of soya proteins and ISF on the production of serum total and SPI-specific antibodies appear to be sex dependent and also related to the age of exposure to soya in rats. However, the physiological significance of these immune responses remains to be determined.