Original Research Article Investigation of Antioxidant Potential in Ocimum basilicum Flower

Aims: The present work is particularly focused on antioxidant properties of flower of Ocimum basilicum plant. Study Design: Study is basically designed on Column chromatography of extracts. Place and Duration of Study: Sample collection and all experimental work was done in Chemistry Department Government College University, Lahore. The study comprises duration of 6 months. Methodology: The flower of Ocimum basilicum were collected, dried and grinded. It was soaked in methanol-water (70:30) in dark bottle for a week. Followed by a scheme (column chromatography). After TLC of extracts, three activities were done. Phosphomolybdate, Ferric thiocyanate (FTC), and Folin-Ciocalteu (FC reagent) for determination of antioxidant capacity, peroxidation, determination of total phenols respectively. Results: The sample OC2 and crude have maximum absorbance at the concentration of 100µl, 200µl and 300µl. The results show that crude has maximum antioxidant capacity. The phenolic contents are in the increasing order of fraction OC2, OC5, and crude. The maximum phenolic contents are present in crude. Reference has the maximum ability for peroxidation for ferric thiocyanate complex by giving red colour. Conclusion: Overall it is concluded that Ocimum basilicum flower has antioxidant capacity as good as a standard antioxidant. It is recommended in food/medicine as natural herbal product.

Recent Advances in Detection, Isolation, and Imaging Techniques for Sulfane Sulfur-Containing Biomolecules

Hydrogen sulfide and its oxidation products are involved in many biological processes, and sulfane sulfur compounds, which contain sulfur atoms bonded to other sulfur atom(s), as found in hydropersulfides (R-S-SH), polysulfides (R-S-Sn-S-R), hydrogen polysulfides (H2Sn), etc., have attracted increasing interest. To characterize their physiological and pathophysiological roles, selective detection techniques are required. Classically, sulfane sulfur compounds can be detected by cyanolysis, involving nucleophilic attack by cyanide ion to cleave the sulfur–sulfur bonds. The generated thiocyanate reacts with ferric ion, and the resulting ferric thiocyanate complex can be easily detected by absorption spectroscopy. Recent exploration of the properties of sulfane sulfur compounds as both nucleophiles and electrophiles has led to the development of various chemical techniques for detection, isolation, and bioimaging of sulfane sulfur compounds in biological samples. These include tag-switch techniques, LC-MS/MS, Raman spectroscopy, and fluorescent probes. Herein, we present an overview of the techniques available for specific detection of sulfane sulfur species in biological contexts.

Competitive Ligand Binding and Photosensitivity Studies of Iron(III)-thiocyanate in presence of Glutamate ion

Addition of a chelating ligand (glutamate ion) to [Fe(SCN)]2+ solution leads to change in the colour. On increasing the glutamate ion concentration in iron thiocyanate complex solution, the colour of [Fe(SCN)]2+ disappears with the emergence of a new peak at lower wavelength due to the formation of [Fe(Glu)]2+complex. The conductance of [Fe(SCN)]2+ complex ion in solution is high while on addition of different concentrations of glutamate ion to iron thiocyanate complex, their conductance value decreases due to formation of [Fe(Glu)]2+. Photosensitivity studies of a series of solutions prepared by the addition of glutamate ion of varying concentrations to ferric chloride-ammonium thiocyanate/potassium thiocyanate solution in the short UV region demonstrate the better stability of [Fe(Glu)]2+compared to [Fe(SCN)]2+ and the rate kinetics of decomposition has been reported.

A binuclear CuII/CaII thiocyanate complex with a Schiff base ligand derived from o-vanillin and ammonia

The new heterometallic complex, aqua-1κO-bis(μ2-2-iminomethyl-6-methoxyphenolato-1κ2 O 1,O 6:2κ2 O 1,N)bis(thiocyanato-1κN)calcium(II)copper(II), [CaCu(C8H8NO2)2(NCS)2(H2O)], has been synthesized using a one-pot reaction of copper powder, calcium oxide, o-vanillin and ammonium thiocyanate in methanol under ambient conditions. The Schiff base ligand (C8H9NO2) is generated in situ from the condensation of o-vanillin and ammonia, which is released from the initial NH4SCN. The title compound consists of a discrete binuclear molecule with a {Cu(μ-O)2Ca} core, in which the Cu...Ca distance is 3.4275 (6) Å. The coordination geometries of the four-coordinate copper atom in the [CuN2O2] chromophore and the seven-coordinate calcium atom in the [CaO5N2] chromophore can be described as distorted square planar and pentagonal bipyramidal, respectively. In the crystal, O—H...S hydrogen bonds between the coordinating water molecules and thiocyanate groups form a supramolecular chain with a zigzag-shaped calcium skeleton.