EFFECT OF PERIURETHRAL INJECTION ADIPOSE-DERIVED STEM CELLS IN NERVE HEALING AND VOIDING BEHAVIOR FOR THE TREATMENT OF STRESS URINARY INCONTINENCE IN A RAT MODEL

Objective: To investigate whether ADSCs are capable of nerve repair in stress urinary incontinence. Material & Methods: Thirty rats were allocated to five groups for receiving a periurethral injection with Phosphate Buffer Saline (PBS) (B1,B2) and Adipose-derived Stem Cells (C1, C2). Functional assessments with Voided Stain on Paper (VSOP) Method were reassessed at 2 and 4 weeks after injection. The rat SUI model was generated by Vaginal Distention (VD). The regeneration of peripheral nerve was evaluated by PGP 9.5 immunohistochemical staining. Results: Results revealed significant peripheral nerve differences between ADSC group and other groups in 28 days using PGP 9.5 staining. Our functional assessment, Voided Stain on Paper (VSOP), showed that ADSC group had significant improvement in 14 days; unfortunately, our 28 days improvement was not substantial. Conclusion: Periurethral injection of ADSCs improves urine pattern in SUI-induced rats and increase the amount of peripheral nerve. This method may be a potential strategy for SUI treatment.

Use of hemostatic drug Spongostan as a carrier of mesenchymal stem cells in the treatment of experimental urinary incontinence in rats

The adhesion and proliferation of rat adipose-derived mesenchymal stem cells (MSCs) as part of biocomposite with haemostatic drug Spongostan Absorbable Haemostatic Gelatin Powder as well as its regenerative efficiency after its introduction into the periurethral region in rats with experimental urinary incontinence were studied. It was found that MSCs rapidly adhere to the surface of the carrier and actively proliferate. The periurethral injection of biocomposite (Spongostan + MSCs) quickly and effectively recovers the urine continence function in animals that have undergone modeling of incontinence by urethrolysis. Spongostan hydrated in the ratio of 1 :24 inphosphate-saline buffer has a volume-forming effect in the urethra of experimental animals.

Stress urinary incontinence: injection method of treatment (literature review)

The purpose of the review - presentation of clinical scientific data based on current evidence of information on the management of patients with stress urinary incontinence, the effectiveness and safety of periurethral injections of various bulking substances. Material and methods. А review of Russian and foreign sources on the prevalence, etiopathogenesis, diagnosis and treatment of stress urinary incontinence in women, in particular the injection method. Results. The review presents data of the etiopathogenesis, diagnosis and treatment of stress urinary incontinence in women, depending on the severity of the pathology. Analyzed the data of periurethral injection of various bulking materials, their effectiveness and safety.