electron microscopical analysis
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2016 ◽  
Vol 201 (3) ◽  
pp. 203-210
Author(s):  
Samir Jabari ◽  
Winfried Neuhuber ◽  
Axel Brehmer

In the 1970s, by using classic histological methods, close topographical relationships between special areas of enteric ganglia and capillaries were shown in the pig. In this study, by application of double and triple immunohistochemistry, we confirmed this neurovascular interface and demonstrated that these zones are mainly confined to nitrergic neurons in the myenteric and the external submucosal plexus. In the upper small intestine of the pig, the respective neurons display type III morphology, i.e. they have long, slender and branched dendrites and a single axon. In another set of experiments, we prepared specimens for electron-microscopical analysis of these zones. Both ganglia and capillaries display continuous basement membranes, the smallest distances between them being 1,000 nm at the myenteric and 300 nm at the external submucosal level. The capillary endothelium was mostly continuous but, at the external submucosal level, scattered fenestrations were observed. This particular neurovascular relationship suggests that nitrergic neurons may require a greater amount of oxygen and/or nutrients. In guinea pig and mouse, previous ischemia/reperfusion experiments showed that nitrergic neurons are selectively damaged. Thus, a preferential blood supply of enteric nitrergic neurons may indicate that these neurons are more vulnerable in ischemia.



2009 ◽  
Vol 62 (5) ◽  
pp. 350-351 ◽  
Author(s):  
Hirokazu Murakami ◽  
Nahoko Hatsumi ◽  
Takayuki Saitoh ◽  
Takeki Mitsui ◽  
Takafumi Matsushima ◽  
...  




2000 ◽  
Vol 299 (4) ◽  
pp. 853-858 ◽  
Author(s):  
Béatrice ten Heggeler-Bordier ◽  
Sylviane Muller ◽  
Marc Monestier ◽  
Walter Wahli




1996 ◽  
Vol 91 (6) ◽  
pp. 573-577 ◽  
Author(s):  
T. Tsuchida ◽  
Masaaki Matsumoto ◽  
Yoshiaki Shirayama ◽  
Takumi Imahori ◽  
Harubumi Kasai ◽  
...  


Parasitology ◽  
1995 ◽  
Vol 110 (5) ◽  
pp. 547-554 ◽  
Author(s):  
S. Tomlinson ◽  
F. Vandekerckhove ◽  
U. Frevert ◽  
V. Nussenzweig

SUMMARYFollowing cell invasion, Trypanosoma cruzi trypomastigotes transform into amastigotes, which are the mammalian replicative forms of the parasite. Although amastigotes represent a critical stage in the life-cycle of T. cruzi, little is known of the factors controlling trypomastigote to amastigote transformation. Kanbera et al. (1990) observed that exposure of trypomastigotes to acidic pH induced their transformation into rounded forms resembling amastigotes. We confirm their observation and, using two strains of T. cruzi, establish that these transformants are ultrastructurally and biochemically indistinguishable from natural amastigotes. Incubation of trypomastigotes in medium at pH 5·0 for 2 h was sufficient to trigger their transformation into forms resembling amastigotes. Electron microscopical analysis confirmed that the kinetoplast structure, and general morphological features of the acid-induced, extracellular amastigotes were indistinguishable from those of intracellular-derived amastigotes. The extracellular transformation was accompanied by the acquisition of the stage-specific surface antigen of the naturally transformed amastigotes (Ssp-4), and loss of a stagespecific trypomastigote antigen (Ssp-3). Trypomastigotes incubated at neutral pH did not transform into amastigotes, and did not acquire the Ssp-4 epitope or lose the Ssp-3 epitope. Finally, acid-induced amastigotes subsequently incorporated [3H]thymidine into their DNA, indicating that the important replicative property of intracellular amastigotes is also exhibited by these in vitro transformants. This effect of low pH appears to be of physiological relevance, and acid-induced extracellular transformation appears to represent a valid experimental technique for studies of the molecular mechanisms involved in the differentiation process.



1995 ◽  
Vol 84 (3) ◽  
pp. 229-229
Author(s):  
Heggeler-Bordier Béatrice ◽  
Wahli Walter


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