transgenic arabidopsis plants
Recently Published Documents


TOTAL DOCUMENTS

154
(FIVE YEARS 24)

H-INDEX

38
(FIVE YEARS 2)

Biology ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1070
Author(s):  
Sivakumar Swaminathan ◽  
Nathan T. Reem ◽  
Vincenzo Lionetti ◽  
Olga A. Zabotina

The plant cell wall (CW) is an outer cell skeleton that plays an important role in plant growth and protection against both biotic and abiotic stresses. Signals and molecules produced during host–pathogen interactions have been proven to be involved in plant stress responses initiating signal pathways. Based on our previous research findings, the present study explored the possibility of additively or synergistically increasing plant stress resistance by stacking beneficial genes. In order to prove our hypothesis, we generated transgenic Arabidopsis plants constitutively overexpressing three different Aspergillus nidulans CW-modifying enzymes: a xylan acetylesterase, a rhamnogalacturonan acetylesterase and a feruloylesterase. The two acetylesterases were expressed either together or in combination with the feruloylesterase to study the effect of CW polysaccharide deacetylation and deferuloylation on Arabidopsis defense reactions against a fungal pathogen, Botrytis cinerea. The transgenic Arabidopsis plants expressing two acetylesterases together showed higher CW deacetylation and increased resistance to B. cinerea in comparison to wild-type (WT) Col-0 and plants expressing single acetylesterases. While the expression of feruloylesterase alone compromised plant resistance, coexpression of feruloylesterase together with either one of the two acetylesterases restored plant resistance to the pathogen. These CW modifications induced several defense-related genes in uninfected healthy plants, confirming their impact on plant resistance. These results demonstrated that coexpression of complementary CW-modifying enzymes in different combinations have an additive effect on plant stress response by constitutively priming the plant defense pathways. These findings might be useful for generating valuable crops with higher protections against biotic stresses.


2021 ◽  
Vol 332 ◽  
pp. 114-125
Author(s):  
Rakesh Manuka ◽  
Ankush Ashok Saddhe ◽  
Ashish Kumar Srivastava ◽  
Kundan Kumar ◽  
Suprasanna Penna

2021 ◽  
Vol 15 (1) ◽  
pp. 13-25
Author(s):  
Saranya Ganapathy ◽  
Megha N. Parajulee ◽  
Michael San Francisco ◽  
Hong Zhang ◽  
Shan L. Bilimoria

2021 ◽  
Vol 1 (1) ◽  
pp. 1-11
Author(s):  
Wei Chen ◽  
◽  
Chao Zheng ◽  
Mingzhe Yao ◽  
Liang Chen ◽  
...  

Author(s):  
Emre Aksoy ◽  
Amir Maqbool ◽  
Buasimuhan Abudureyimu

Iron (Fe) is an important trace mineral for plant development, and plants grown in Fe deficiency experience yield losses due to the leaf chlorosis. In addition to agronomic measures that can be taken to minimize these losses, new plant genotypes can be developed effectively through genetic engineering. While dicots such as Arabidopsis thaliana use a reduction-based strategy to uptake high amounts of iron from the rhizosphere, the chelation strategy has evolved in Gramineous plants including barley (Hordeum vulgare). In this study, barley NICOTIANAMINE SYNTHASE1 (HvNAS1) gene, which is responsible for the production of nicotianamine that can complex with iron, was cloned and expressed at a constitutive high level in Arabidopsis plants. The expression levels of Arabidopsis genes encoding for the proteins involved in iron uptake increased together with HvNAS1 in the T3 Arabidopsis plants. Moreover, the root lengths, root and stem fresh weights, ferric chelate reductase enzyme activities of the plants also increased in the transgenic Arabidopsis plants under Fe deficiency. In addition, significant increases in iron and zinc levels were determined in the roots and shoots of transgenic Arabidopsis plants. As a result, transgenic Arabidopsis plants overexpressing the barley HvNAS1 gene can take up more iron from the rhizosphere and carry this iron to the shoots. This study demonstrates the power of genetic engineering to develop Arabidopsis plants overexpressing the HvNAS1 gene and therefore tolerate iron deficiency.


Sign in / Sign up

Export Citation Format

Share Document