The enteric nervous system of C. elegans is specified by the Sine Oculis-like homeobox gene ceh-34

Overarching themes in the terminal differentiation of the enteric nervous system, an autonomously acting unit of animal nervous systems, have so far eluded discovery. We describe here the overall regulatory logic of enteric nervous system differentiation of the nematode C. elegans that resides within the foregut (pharynx) of the worm. A Caenorhabditis elegans homolog of the Drosophila Sine Oculis homeobox gene, ceh-34, is expressed in all 14 classes of interconnected pharyngeal neurons from their birth throughout their life time, but in no other neuron type of the entire animal. Constitutive and temporally controlled ceh-34 removal shows that ceh-34 is required to initiate and maintain the neuron type-specific terminal differentiation program of all pharyngeal neuron classes, including their circuit assembly, without affecting panneuronal features. Through additional genetic loss of function analysis, we show that within each pharyngeal neuron class, ceh-34 cooperates with different homeodomain transcription factors to individuate distinct pharyngeal neuron classes. Our analysis underscores the critical role of homeobox genes in neuronal identity specification and links them to the control of neuronal circuit assembly of the enteric nervous system. Together with the pharyngeal nervous system simplicity as well as its specification by a Sine Oculis homolog, our findings invite speculations about the early evolution of nervous systems.

Emulation of Circuits under Test Using Low-Cost Embedded Platforms

Electrical engineering education requires the development of the specific ability and skills to address the design and assembly of practical electronic circuits, as well as the use of advanced electronic instrumentation. However, for electronic instrumentation courses or any other related specialty that pursues to gain expertise testing a physical system, the circuit assembly process itself can represent a bottleneck in a practical session. The time dedicated to the circuit assembly is subtracted both to the measurements and the final decision-making time. Therefore, the student’s practical experience is limited. This article presents a reconfigurable physical system based on the Arduino™ shield pin-out, which (after specific programming) can virtually behave as a device under test to carry out measurement procedures on it, emulating any system or process. Although it has been mainly oriented to the Arduino boards, it is possible to add different control devices with a connector compatible. The user does not need to assemble any circuit. Our approach does not only pursue the correct instrument handling as a goal, but it also immerses the student in the context of the functional theory of the proposed circuit under test. Consequently, the same emulation platform can be utilized for other techno-scientific specialties, such as electrical engineering, automatic control systems or physics courses. Besides that, it is a compact product that can be adapted to the needs of any teaching institution.