CO2 emissions and scarcity of petroleum supplies can be reduced by the use of renewable energy sources. This has led to the development of low emission aviation turbine fuel derived from vegetable materials. The process used Hydroprocessed Esters and Fatty Acids (HEFA) that consisted of hydrotreating and isomerization. This experiment consisted of production and analysis. The production of aviation biofuel developed based on coconut oil through the process of hydrotreating deoxygenation (HDO) and hydrotreating isomerization (HDI) on the hydrotreater reactor. Further analysis of aviation biofuel products and aviation biofuel blending includes Total Acid Number (TAN), existent gum, density at 15 °C, flash point, smoke point, freezing point, distillation, total sulfur content, distribution of C10-C14 carbon, and levels of Iso-Paraffin Olefin Naphthene (PION). Based on the experimental results, it can be concluded that the Aviation Biofuel complied for TAN, density at 15 °C, and freezing point parameters. Aviation Biofuel blending of 2%, 5%, and 10% were in accordance with the Aviation Biofuel Defence Standard 91-91 for all parameters.