Genetic structure and temporal environmental niche dynamics of sideoats grama [Bouteloua curtipendula (Michx.) Torr.] populations in Mexico

In the past years, several plant breeding programs have been done to select outstanding genotypes of sideoats grama (Bouteloua curtipendula) for restoration purposes. Such programs have been focused mainly on agronomic traits; however, little attention has been paid to the genetic structure and environmental adaptation of the selected genotypes. Thus, in this study we evaluated the genetic structure of 85 sideoats grama populations in Mexico. In addition, we modeled the past, present and future environmental niche of the genetic clusters of this species. Ninety sideoats grama populations were genetically analyzed through AFLP (Amplified Fragment Length Polymorphisms) markers. The environmental niche of the population clusters was modeled by using the maximum entropy method. The genetic analysis separated the populations into two genetically different clusters (p = 0.0003). The differentiation of these lineages can be partially explained by the paleoclimatic events experienced during the last interglacial and glacial maximums. Consequently, the genetic clusters have different environmental niche at the present time. Suitability areas for the distribution of Cluster I are mainly located in the central part of the country while the environmental niche of Cluster II is located in the semiarid region, close to the mountain range of the Sierra Madre Occidental. Thus, selection and restoration programs with sideoats grama must be carried out using local germplasm from each environmental niche. Given the environmental niche of both genetic clusters will suffer changes in the near and mid-century future, climate change must be considered for genotypes selection and restoration programs.

Reducing Eragrostis lehmanniana populations by preparing seedbeds with unconventional tillage implements and seeding in a semiarid grassland

The invasion of Lehmann lovegrass (Eragrostis lehmanniana Nees) in rangelands of Chihuahua, Mexico, has resulted in a need for revegetation to recover lost forage productivity. Thus, new knowledge on generating alternatives to improve these invaded grasslands is of great importance. This study evaluated seedbeds prepared with unconventional tillage implements and seeded with a grass mixture to reduce the plant density of E. lehmanniana while increasing the productivity of an invaded semiarid grassland of Chihuahua. The unconventional tillage implements were: a Rangeland Harrow, which was used to prepare the Striped Harrowing and Full Harrowing seedbeds; Rangeland Rehabilitator, which was used to prepare the Deep-Stingray Subsoiler seedbed; and a Tandem-type Aerator Roller, which was used to prepare the Double-Digging Aeration seedbed. An area without tillage was left as a control. The seed mixture was composed of blue grama [Bouteloua gracilis (Willd. ex Kunth) Lag. ex Griffiths var. Hachita] (25%); sideoats grama [Bouteloua curtipendula (Michx.) Torr. ‘6107 Kansas’] (25%); green sprangletop [Leptochloa dubia (Kunth) Nees var. Van Horn] (5%); weeping lovegrass [Eragrostis curvula (Schrad.) Nees var. Ermelo] (40%), and Columbus grass [Sorghum almum Parodi] (5%). The experiment was conducted across 4 yr, and the evaluation started at the second year. Plant density and dry matter (DM) production were evaluated per species. In the control plot, the plant density of E. lehmanniana increased approximately 180% from the 2nd to the 4th year (18 to 50 plants m−2). The use of unconventional tillage implements for seedbed preparation and the inclusion of E. curvula in the seed mixture decreased E. lehmanniana density in more than 50% of plots and increased DM production in around 100% of plots. Considering the whole experimental period, in all the prepared seedbed treatments, E. curvula had the highest establishment and DM production of all the seeded species. The native species B. gracilis, B. curtipendula, and L. dubia had poor establishment in all the prepared seedbeds.

Diflufenzopyr Influences Leafy Spurge (Euphorbia esula) and Canada Thistle (Cirsium arvense) Control by Herbicides

Diflufenzopyr is an auxin-transport inhibitor that has increased broadleaf weed control by some auxin herbicides. The effects of auxin herbicides when applied with diflufenzopyr for leafy spurge or Canada thistle control, herbage production, and herbicide absorption and translocation in leafy spurge were evaluated. The influence of diflufenzopyr on leafy spurge and Canada thistle control varied by herbicide. Diflufenzopyr applied with quinclorac increased both Canada thistle and leafy spurge control. Diflufenzopyr added to picloram increased leafy spurge but not Canada thistle control, whereas control of both weeds generally increased when diflufenzopyr was applied with dicamba. Canada thistle control slightly improved when diflufenzopyr was applied with clopyralid or clopyralid plus 2,4-D compared with the herbicides alone. Diflufenzopyr did not influence weed control from imazapic and reduced weed control from glyphosate. Weed control was not influenced by the ratio of diflufenzopyr to herbicide or by whether diflufenzopyr was tank mixed with or applied before the herbicides. Diflufenzopyr did not affect cool-season grass production in the greenhouse or field but did reduce sideoats grama and switchgrass production when applied alone and with dicamba in greenhouse trials.14C-picloram and14C-quinclorac absorption was increased when applied with diflufenzopyr in leafy spurge but nearly all absorbed herbicide remained in the treated leaf compared with the herbicides applied alone.14C-dicamba absorption decreased from 60 to 14% when applied with diflufenzopyr and translocation to the roots decreased by a factor of 10. Overall, the increase in weed control from the addition of diflufenzopyr was much more pronounced with leafy spurge than with Canada thistle.