Features of saliva diagnostics and threshold gustometry in patients with non-alcoholic fatty liver disease

Objective — to determine the features of dental complaints and the threshold of taste sensitivity of the tongue receptors to glucose in patients with non‑alcoholic fatty liver disease (NAFLD) against the background of insulin resistance. Materials and methods. The study included 65 patients (29 (44.6 %) women and 36 (55.4 %) men) with NAFLD (the main group) and 24 somatically healthy subjects. The mean body mass index was 36.4 kg/m2 in the main group, and 22.8 kg/m2 in the controls. All patients had insulin resistance (НОМА index — 8.0). Dental examinations included questionnaires of patients with complaints’ detailing, clinical examination of the oral cavity, study of salivation rate, determination of the sensitivity of the taste receptors of the tongue to glucose by the method of gustometry. Results. It has been established that the mostly frequent complaints of patients in the main group were constant dry mouth, taste distortion and halitosis. They demonstrated a decrease in salivation rate up to 0.24 ± 0.13 ml per minute, changes in the taste sensitivity of the tongue to sweet, that was several times higher than the threshold of the physiological norm. The strong direct correlation has been proved between oral dryness and salivation rate. Correlations of moderate strength were confirmed between the secretion rate and glucose concentration according to gustometry, and between taste distortion and halitosis. Conclusions. Almost 90 % of patients with non‑alcoholic fatty liver disease have typical dental complaints. Questioning patients on the main dental complaints, studying the parameters of the oral fluid and threshold gustometry are the modern, non‑invasive methods, enabling the control of the disease course and assessment of the treatment efficacy.

Salivary inflammatory biomarkers and glycated haemoglobin among patients with type 2 diabetic mellitus

Background Type 2 diabetes mellitus has reached epidemic proportions worldwide and improved detection techniques and biomarkers are urgently needed across the spectrum of diabetes initiation and progression. Inflammatory biomarkers play a role in the development of the condition and blood is the gold standard body fluid for the diagnosis of diabetes mellitus. Serum glycated haemoglobin is a widely used marker of chronic hyperglycemia, and it is currently used to diagnose type 2 diabetes mellitus and it is the standard biomarker for the adequacy of management. However, saliva offers an alternative to serum as a biological fluid for diagnostic purposes. Non-invasive measures of inflammatory biomarkers (such as saliva diagnostics) are increasingly being investigated due to significant similarities between salivary and serum proteome. The role of saliva diagnostics in diabetes mellitus has not been explored in our study population. Objectives This study investigated the association of selected salivary inflammatory biomarkers (Interleukin 6 [IL-6], C-reactive protein [CRP], and Tumour necrosis factor α [TNF-α]) to glycated haemoglobin (HbA1C) in type 2 diabetics. Materials and methods Seventy-five participants, 39 type 2 diabetics (52%) and 36 (48%) healthy controls were recruited. Saliva and blood samples were collected for each participant. The levels of selected salivary inflammatory biomarkers (IL-6, CRP and TNF-α) were estimated by Enzyme Linked Immunosorbent Assay (ELISA) method and glycated haemogloin (HbA1C) was estimated using the liquid chromatography method. Periodontal status of the participants were determined using the Basic Periodontal Examination (BPE). Results The mean salivary levels of CRP was significantly higher in diabetics, 0.05 ± 0.04 µg/ml than in controls, 0.02 ± 0.02 µg/ml (p < 0.001). Mean TNF-α was also significantly higher in diabetics, 5.39 ± 12.10 pg/ml than in controls, 1.51 ± 3.66 pg/ml (p = 0.036). Mean salivary IL-6 was also higher in diabetics compared with controls (47.20 ± 18.49 versus 41.94 ± 16.88 pg/ml), but the difference was not statistically significant, p = 0.204. In the multivariate analysis adjusting for age and periodontal status, only the mean salivary CRP was significantly higher in diabetics, 0.034 higher than controls (95% CI 0.009, 0.059 and p = 0.01). There was a positive correlation between salivary CRP and HbA1C levels, which was moderate with r-value 0.4929 and p-value < 0.0001. Conclusions Salivary inflammatory biomarkers especially CRP are higher in diabetics compared with controls and CRP is positively correlated with serum HbA1C levels. The biomarkers show potentials as non-invasive alternative method to evaluate glycaemic control in diabetes.

ДІАГНОСТИЧНА ІНФОРМАТИВНІСТЬ САЛІВАРНИХ ПОКАЗНИКІВ ГОМЕОСТАЗУ РОТОВОЇ ПОРОЖНИНИ ПРИ СТОМАТОЛОГІЧНІЙ ТА СУПУТНІЙ ПАТОЛОГІЯХ У ДІТЕЙ (ОГЛЯД ЛІТЕРАТУРИ)

The article highlights the role of diagnostics of saliva in maintaining oral homeostasis as quality criteria of children’s health.The aim of the study – to conduct the analysis of modern domestic and foreign literature in relation to perspective of diagnostics of oral liquid indexes of the antioxidant system in children.Materials and Methods. The research used bibliosemantic and analytical methods.Results and Discussion. During the study, the latest scientific domestic and foreign medical literature data on the issue of saliva diagnostics in maintaining oral homeostasis for children with stomatological pathologies on a background of concomitant diseases were studied and analyzed.Conclusions. Nowadays, the saliva indexes of oral homoeostasis can be used as markers of violation of general state, intensity of pathological processes, monitoring of conducted treatment efficiency and also expansion of ideas about etiology and pathogenesis of stomatological and somatic diseases in children.