Analysis of Runoff Variation Characteristics and Influencing Factors in the Wujiang River Basin in the Past 30 Years

Changes in climate and the underlying surface are the main factors affecting runoff. Quantitative assessment of runoff characteristics, and determination of the climate and underlying surface contribution to changes in runoff are critical to water resources management and protection. Based on the runoff data from the Wulong Hydrological Station, combined with the Mann-Kendall test, Indicators of Hydrologic Alteration (IHA), Budyko hypothesis, and changes in climate and the underlying surface, this study comprehensively analyzed the runoff in the Wujiang River Basin (WRB). The results showed that: (1) The annual runoff of Wujiang River showed a downward trend, and an abrupt change occurred in 2005. (2) The overall hydrological change in WRB is 46%, reaching a moderate change. (3) The contribution rates of precipitation (P), potential evaporation (ET0), and underlying surface to runoff changes are 61.5%, 11.4%, and 26.9%, respectively. (4) After 2005, the WRB has become more arid, human activities have become more active, vegetation coverage has increased, and the built-up land has increased significantly.

Triplophysa wulongensis, a new species of cave-dwelling loach (Teleostei, Nemacheilidae) from Chongqing, Southwest China

We describe a new species of cave-dwelling loach, Triplophysa wulongensissp. nov., based on specimens collected in a subterranean pool in a cave in Wulong County, Chongqing, Southwest China. The pool is connected to the Wujiang River drainage. Triplophysa wulongensis differs from its congeners by the following combination of characters: eyes present, caudal fin with 18 branched rays; posterior chamber of the air bladder degenerate; stomach U-shaped; intestine without bends or loops immediately posterior to stomach; body smooth and scaleless, and lateral line complete. The mitochondrial cytochrome b sequence differs from those of other published sequences of species of Triplophysa by 14.9–24.9% in K2P distance. Phylogenetic analysis based on cytochrome b gene sequences recovered T. wulongensis as sister taxon to all other cave-dwelling species of Triplophysa.

A method for the analyses of carbon stable isotope and content of particulate inorganic carbon in inland waters

<p>Studies on particulate inorganic carbon (PIC) in inland waters are relatively scarce due to the low concentration of PIC which makes it difficult to be measured accurately. In other studies, a characteristic ratio of PIC in total suspended solids in the water column has been proposed to estimate the river PIC flux to the sea, and a titration method to measure the PIC fluxes in karst rivers has been reported. Therefore, we used the Gas Bench Ⅱ-IRMS coupled technique method to analyze the δ<sup>13</sup>C<sub>PIC</sub> and PIC concentration in inland waters. The method has the advantage of being suitable for the accurate determination of the isotopic composition of trace PIC samples.</p><p>The purging time and carbon content of samples are the important factors affecting experimental accuracy. This study proposed the optimal purge time and the lowest carbon content of the inland water sample. The samples in the experiment included laboratory calcium carbonate standard (99.95 % purity) and PIC samples from the Wujiang River. The PIC samples from Wujiang River were collected on glass fiber filters. Datasets from the experiment demonstrated that the ideal purge time is 500-700 s, and at least 25 μg C should be included in the sample. The instrument signal value is low and the isotopic value fluctuates widely when the purge time is less than 500 s. The phosphoric acid cannot be injected into the sample bottle due to the high pressure in it when the purge time is more than 700 s. Therefore, a purging time of 600 s was used for the field sample analyses. The peak area displayed by the device is correlated with the carbon content in the sample, and the datasets show a good linear relationship between the peak area and carbon content in the sample when the sample be analyzed contained more than 25 μg of inorganic carbon. The carbon content of the sample can be calculated from the peak area of the same batch of calcium carbonate standard. While the peak signal is too low to detect the sample accurately when the C content is less than 25 μg. Therefore, the sample should contain more than 25 μg for the field sample analyses. This study will help to provide a reference for the method of determining the PIC content and isotopic composition in inland water.</p>

Interaction between carbon cycling and phytoplankton community succession in hydropower reservoirs: Evidence from stable carbon isotope analysis

<p><strong>Abstract:</strong> Carbon (C) cycling and phytoplankton community succession are very important for hydropower reservoir ecosystems; however, whether the former controls the latter or the reverse is still debated. To understand this process, we investigated phytoplankton species compositions, stable C isotope compositions of dissolved inorganic C and particulate organic C (δ<sup>13</sup>C-DIC and δ<sup>13</sup>C-POC), and related environmental factors in seven hydropower reservoirs on the Wujiang River, Southwest China. A total of 36 algal genera from seven phyla were identified, and phytoplankton community exhibited obvious temporal and spatial difference. The δ<sup>13</sup>C-DIC (from -9.96 to -3.73‰) and δ<sup>13</sup>C-POC (from -33.44 to -21.17‰) co-varied with the algal species succession and increased markedly during the shift of dominant species from Bacillariophyta to Pyrrophyta or Cyanophyta. In addition, the strong C fixation in the euphotic layer resulted in great δ<sup>13</sup>C-DIC and CO<sub>2</sub> stratification in the reservoir profile. Statistical analyses and C isotope evidence demonstrate that an increase in water temperature triggers phytoplankton community succession, and that CO<sub>2</sub> availability is a key to drive the succession direction, and in turn, C cycling is enhanced when phytoplankton are dominated by Pyrrophyta or Cyanophyta in hydropower reservoirs. This study confirms that C cycling and phytoplankton community succession interact with each other and evolve synchronously, and will be helpful to systematically evaluate the environmental consequences of river damming.</p><p><strong>Keywords: </strong>Carbon biogeochemical cycling; Phytoplankton community succession; Stable carbon isotope; Reservoir effect; Wujiang River.</p>