Phylogeography of Nasutitermes ephratae (Termitidae: Nasutitermitinae) in Neotropical region

The neotropical region ranks third in the number of termites with five different families. Of these, Termitidae is the most diverse and includes the species Nasutitermes ephratae and is common in the neotropics. To date, only one study has been published about phylogeographic issues in neotropical termites (N. corniger). Here, we aimed to investigate and analyze the population genetic patterns of N. ephratae and then evaluated the phylogeographical processes involved in the evolutionary history of the species. We used the mitochondrial genes 16S rRNA and COII as molecular markers: These were sequenced for 128 samples of N. ephratae. We estimated the genetic diversity and divergence time as well as the demographic and genetic structure analyses. We also produced ancestral area reconstruction and a haplotype network. The results showed high genetic variability, recent demographic expansion, and strong genetic structure. We also inferred a dispersal route for the species that occurred in both directions between South and Central America. The results emphasize a temporary separation between the South and Central America population that affected the origin of the current Central America populations. These were formed form different phylogeographic histories.

Quantifying Airborne Dispersal Route of Corynespora cassiicola in Greenhouses

Target leaf spot (TLS), caused by Corynespora cassiicola, is an emerging and high-incidence disease that has spread rapidly on the global scale. Aerospores released by infected plants play a significant role in the epidemiology of cucumber TLS disease; however, no data exist concerning the infectiousness and particle size of C. cassiicola aerospores, and the experimental evidence for the aerospores transmission was lacking. In the present study, highly effective approaches to collect and quantify aerospores were developed for exposure chamber and greenhouse studies. Quantifiable levels of C. cassiicola aerospores were detected in 27 air samples from nine naturally infested greenhouses, ranging from 198 to 5,969 spores/m3. The C. cassiicola strains isolated from air samples were infective to healthy cucumber plants. Exposure chambers were constructed to study the characteristics of C. cassiicola aerospores released by artificially infested cucumber plants. The particle size of C. cassiicola ranged predominately from 2.1 to 4.7 μm, accounting for 71.97% of the total amount. In addition, the transmission dynamics of C. cassiicola aerospores from donor cucumber plants to recipient cucumber plants were confirmed in exposure chambers and greenhouses. The concentration of C. cassiicola aerospores was positively associated with cucumber TLS disease severity. This study suggested that aerospore dispersal is an important route for the epidemiology of plant fungal disease, and these data will contribute to the development of new strategies for the effective alleviation and control of plant diseases.

First report of Megachile (Callomegachile) sculpturalis Smith, 1853 (Apoidea: Megachilidae) from India

The giant resin bee, Megachile (Callomegachile) sculpturalis (Smith, 1853) is an invasive solitary bee that has spread all over North America and Europe in the last decade. Although native to Asia, not much is known about its distribution in this continent. In the present study, Megachile (Callomegachile) sculpturalis is reported for the first time from India. Related information about this species regarding its nesting habits, floral association, and impact in introduced range obtained through bibliographical research along with the possible dispersal route after introduction in South Eastern Asia is provided. The checklist of Megachile (Callomegachile) in India is also provided. Key words: Pollinator, giant resin bee, range extension, Northeast India

Late Paleogene herpetofaunas from the crossroads between two continents – new amphibian and reptile remains from the Oligocene of southern Balkans and Anatolia

We describe here new amphibian and reptile remains from three Oligocene localities of Turkey. Two of the localities (Kavakdere and Kocayarma) are situated in southeastern Europe and the other one (Kargi 2) in Anatolia, both areas where Oligocene herpetofauna is practically almost unknown. The material consists of albanerpetontids, pelobatid anurans, turtles, crocodylians, lacertids, scinciformatans, anguines, and “tropidophiids”. Albanerpetontids are for the first time identified in southeastern Europe, with the material being reminiscent of the younger species Albanerpeton inexpectatum Estes & Hoffstetter, 1976; the material potentially represents the oldest record of that species. Pelobatids, scinciformatans, and “tropidophiids” represent the oldest occurrences of these clades in the northeastern Mediterranean. The anguine genus Ophisaurus Daudin, 1803 is identified for the first time in the Paleogene of Eastern Europe. The “tropidophiids” are referred to two genera, Falseryx Szyndlar & Rage, 2003 and tentatively also to Platyspondylia Rage, 1974, with the latter having been so far exclusively known from western and central Europe. The role of a potential southern dispersal route of taxa among Asia and Europe, involving the area of southern Balkans and Anatolia, similarly to what has been recently demonstrated for mammals, is highlighted also for amphibians and reptiles.

An initial record of a long-distance dispersal route of a male sika deer in central Japan

We conducted a global positioning system (GPS) tracking of a male sika deer (Cervus nippon) and his mother in the eastern foothills of the northern Japanese Alps, central Japan. Sika deer exhibited similar seasonal movement patterns; however, the male deer left his natal group at 11 months of age. At 15 months of age, the male deer settled in the neighboring mountain, which was 74 km away from his natal range. This is the first record of long-distance (>50 km) natal dispersal of the sika deer. Our findings might help to explain the expanding distribution of the sika deer.