Mapping Panlongcheng: New Work on the Type-Site of the Early Shang Period (1500–1300 BC) in Hubei Province, China

Recent work at the early Shang period type site in Panlongcheng, Hubei Province, China, provides a new understanding of changes in the landscape and water environment over time. In the past few decades, the research at this site has obtained important results and shown progress in many aspects, but few scholars have discussed the geomorphological environment of Panlongcheng, especially the water environment. Researchers have long believed that the present-day environment and landscape of Panlongcheng are no different than during the early Shang period. However, recent archaeological discoveries indicate that there may still be some cultural remains underwater. Therefore, we used a combination of underwater surveys, drilling and digital mapping to expand our knowledge of the landscape of Panlongcheng during the early Shang period. This included mapping the lake basin using single-beam echo sounders and drilling to preliminarily observe the stratum and collect samples from underwater. We also conducted radiocarbon dating on the samples collected from the bottom of the lake. The results suggest that there might not have been a lake during the early Shang period. Therefore, the landscape and environment of Panlongcheng and other related issues should be reexamined. In addition, we hope the methods used in this study can provide a reference for related archaeological work in shallow water areas in inland China.

Archaeology of the Kerma Culture

Kerma was a Bronze Age culture (c. 2500–1500 bce) located in what is today Sudan and southern Egypt. It is one of the earliest complex societies in Africa and, at its height, rivaled Ancient Egypt. The ancient Kerma culture spans the Pre-Kerma, examining the settlements and cemeteries of this ancient culture during the Pre-Kerma (3500–2500 bce, included here as a precursor to the Kerma civilization), Early Kerma, Middle Kerma, Classic Kerma, and Recent Kerma periods. Much of what is known comes from the capital city and type site, Kerma. However, other urban centers such as Sai, as well as hinterland communities, are also discussed. An archaeological approach is crucial to the examination of Kerma’s past because an indigenous writing system had not yet been developed. Interaction with Egypt is discussed, but only as it relates to Kerma’s historical context. Chronological changes to craft production, religious practices, domestic spaces, and funerary rituals are framed by larger sociopolitical and socioeconomic issues, including inequality, political authority, and economic development.

Stable isotopes confirm the Banwell Bone Cave Mammal Assemblage Zone represents an MIS 5 fauna

The position of the Banwell Bone Cave mammal assemblage zone (MAZ) in the mammalian biostratigraphy of the British Isles has been the focus of debate for decades. Dominated by fauna typical of cold environments it was originally linked to the marine oxygen isotope stage (MIS) 4 stadial (ca. 72–59 ka). Subsequently it was argued that the Banwell Bone Cave MAZ more likely relates to the temperate interstadial of MIS 5a (ca. 86–72 ka). It is envisioned that “cold fauna” such as bison and reindeer moved into Britain during stadial MIS 5b (ca. 90 ka) and were subsequently isolated by the rising sea level during MIS 5a. Here we investigate environmental conditions during the Banwell Bone Cave MAZ using bone collagen δ13C and δ15N and tooth enamel δ18O and δ13C isotope analysis. We analyse bison and reindeer from the MAZ type-site, Banwell Bone Cave. Our results show unusually high δ15N values, which we ascribe to arid conditions within a temperate environment. Palaeotemperature estimates derived from enamel δ18O indicate warm temperatures, similar to present day. These results confirm that the Banwell Bone Cave MAZ relates to a temperate interstadial and supports its correlation to MIS 5a rather than MIS 4.

Tismice jako produkční a nadregionální centrum Čech 8. a 9. století Tismice as a Production and Supra-Regional Centre of Bohemia in the 8th and 9th Centuries

The central Bohemian hillfort-type site in Tismice is one of the largest (20–22 ha) in Bohemia from the Early to Middle Hillfort period (8th–9th century) and has been studied for years using non-destructive methods. The study, which presents the results of the complete geophysical survey of the site as well as the results of small-scale trenching from 2013, is focussed on an analysis of non-ferrous metal ornaments (c. 170 pcs., mostly belt and horse harness decorations) and analyses of evidence of craft production – jewellerymaking, metalsmithing and metal casting (ingots, blanks). It presents the earliest evidence of work with gold in early medieval Bohemia (2018 excavation) as well as natural science radiocarbon and magnetic absolute dates, which supplement dating on the basis of Carolingian coin and imports. The internally divided hillfort was an elite residence (gilded ornaments, spurs, coin) and for a short time served as a supra-regional centre that not only received and redistributed but also produced items of statutory importance (belt and horse harness ornaments, pendants, spurs). Radiocarbon dating places the multiphase early medieval settlement and fortifications in the period from the final third of the 8th century until the end of the 9th century. A detailed evaluation of the stratigraphy will be another step towards a comprehensive interpretation of this site. Key Words: Early Middle Ages, hillfort, Avar period metal ornaments, spurs, long-distance contacts, goldsmithing, metalsmithing tools, Carolingian coin, central Bohemia

Abundance of Viscum in Central Poland: Results from a Large-Scale Mistletoe Inventory

Mistletoe (Viscum album L.) is a semi-parasitic plant, and in moderate abundance, it does not pose a lethal threat to a tree. However, recent droughts have made a heavy impact on forests, and in recent years, mistletoe began to appear on a larger scale in regions and in places where it has never been a threat before. The main purpose of this research is to carry out a large-scale mistletoe inventory in central Poland for assessing the number of mistletoe on individual trees and in the stands, as well as to test a new inventory method adapted to Polish conditions. It is based on random sample plots, each consisting of 15 trees on which the impact of mistletoe is assessed. On every sample plot, the mistletoe occurrence, location in the crown, estimate of the percentage within the crown, and the number of mistletoe on a tree are assessed. In addition, for further analysis, information about stands (forest habitat type, site index) as well as measured trees (age, diameter, Kraft class, crown length, loss of assimilation apparatus, crown type) levels is recorded. Research undertaken in Kozienice Forest (100 km south from Warsaw) took into account 100 sample plots on which 1500 trees were assessed. Preliminary results show that 16% of trees are infected by mistletoe, more than 25% of which are infected heavily by more than 6 specimens.

Propylene glycol-based antifreeze as an effective preservative for DNA metabarcoding of benthic arthropods

Maintaining the integrity of DNA in bulk environmental samples from source to laboratory is crucial for capturing the true range of taxa present within an ecosystem. Preservation consideration of DNA is particularly important if samples are being collected in remote areas and by non-specialist ‘citizen scientists’ in nationwide programs. Traditionally, absolute ethanol is used as the preferred preservative for environmental samples collected for downstream DNA metabarcoding analyses. However, transport, shipping and DNA extraction of samples preserved in ethanol is a lengthy procedure due to safety restrictions and the requirement of full ethanol evaporation prior to extraction. We examined the efficacy of an easily accessible, non-toxic propylene glycol-based antifreeze as an alternative to absolute ethanol for preserving macroinvertebrate DNA from bulk-benthos DNA samples. We tested the differences in both cytochrome oxidase I (COI) exact sequence variants (ESVs) and COI taxonomic orders detected in both ethanol and antifreeze samples using two processing methods (no evaporation of preservative versus full evaporation). In addition, we assessed the detection of families and genera within the Arthropoda phylum for preservative type, site and processing method. Our results suggest that antifreeze is a suitable alternative to ethanol, a greater global ESV richness reported for antifreeze samples. Additionally, a higher proportion of arthropod reads in ESVs were detected in antifreeze (average 69%) compared with ethanol (average 53%). Finally, antifreeze samples produced similar results for the different processing methods, whereas ethanol samples failed to produce similar results without prior evaporation. Although ethanol is currently widely used for DNA preservation, our results demonstrate that by using antifreeze, it is possible to achieve similar taxonomic coverage and community assemblages of bulk-benthos DNA samples for macroinvertebrates, with the added simplicity and shorter laboratory processing time achieved using an easily available, unregulated preservative.

Biocompatibility and Carcinogenicity of Carbon Nanotubes as Biomaterials

With the development of nanotechnology in recent years, there have been concerns about the health effects of nanoparticles. Carbon nanotubes (CNTs) are fibrous nanoparticles with a micro-sized length and nano-sized diameter, which exhibit excellent physical properties and are widely studied for their potential application in medicine. However, asbestos has been historically shown to cause pleural malignant mesothelioma and lung cancer by inhalation exposure. Because carbon nanotubes are also fibrous nanotubes, some have raised concerns about its possible carcinogenicity. We have reported that there is no clear evidence of carcinogenicity by local and intravenous administration of multi-walled CNTs to cancer mice models. We firmly believe that CNTs can be a safe, new, and high-performance biomaterials by controlling its type, site of administration, and dosage.