Monitoring of veterinary pharmaceuticals in the Bienne river (Jura Mountain, France)

<p>The Bienne river (Jura Mountain, France) drains a basin of medium altitude mountains characterized by extensive cattle breeding (mostly dairy cows). A monitoring of the contamination by veterinary pharmaceuticals was performed using passive sampling devices - POCIS (Polar Organic Chemical Integrative Samplers), between September 2019 and January 2020. Four hydrological conditions were analysed: a sever low-flow periods, two flood events and a winter situation close to the mean interannual flow. Each time, POCIS were exposed over 2 weeks at 2 stations located in the upper and downstream reaches of the river. 19 pharmaceuticals were selected from information given by local veterinarians and analysed by LC MS-MS: endo and ectoparasites treatments; antibiotics and non-steroidal anti-inflammatory drugs. The monitoring shows that most of these chemicals (12 substances) are quantified in all POCIS samples and the others show relatively high occurrences, between 25 and 88%. Average concentrations in water (calculated with the sampling rate i.e. considering the time of exposition of POCIS samplers in the river) are remarkably close between the 2 monitoring stations. Concentrations are high all over the studied period and reach a maximum during flood events. Thus, hazardous effects are expected on freshwater organisms, especially for macrocyclic lactones and pyrethroids and organophosphates pesticides. The antibiotics concentrations ranges can also disturb microbial communities existing in the river. Such results highlight an important impregnation by these pharmaceuticals at the catchment scale, involving diffuse sources as grasslands receiving contaminated cow dungs and manures. Veterinary compounds are strongly remobilized during rain episodes by run off and infiltration in soils. In the hydrogeological context of the Bienne basin, karstic flows emphasize the connectivity between grasslands and the river. Therefore, an important part of the contaminated leaching waters can rapidly reach the river via the soil drains and surface / subsurface flows. Rather than another part goes through less porosity pathways and delivers pollutants over a longer period. Wastewater discharges and sludges from rural and urban treatment plants can also contribute to this pollution for pharmaceuticals also used in human medications.</p>

Contour Lines Generation in Karstic Plateaus for Topographic Maps

<p><strong>Abstract.</strong> Contour lines are a key features of topographic maps as they make the comprehension of terrain more easy. But they are no longer drawn by cartographers, they are mostly automatically derived from digital terrain models. Despite real progress in this automated derivation, some specific terrain landscapes remain incorrectly depicted with such techniques, and this is the case for karstic plateaus full of sinkholes. This paper proposes a specific automated method to derive better contour lines in plateaus, particularly around sinkholes. The process first detects karstic plateaus with many sinkholes, as well as the individual sinkholes. Then, the DTM is smoothed to better reflect the terrain in the plateau and in its surroundings. As a third step, the contour lines around sinkholes are enhanced to draw legible round features that better reflect the real terrain. The process was implemented in a QGIS plugin and tested on a small area with a karstic plateau in the Jura mountain in France, and the cartographers of IGN, the French national mapping agency assessed the results as a great improvement compared to the generic automated process to derive contour lines.</p>

Spatial density estimates of Eurasian lynx (Lynx lynx) in the French Jura and Vosges Mountains

Obtaining estimates of animal population density is a key step in providing sound conservation and management strategies for wildlife. For many large carnivores however, estimating density is difficult because these species are elusive and wide-ranging. Here, we focus on providing the first density estimates of the Eurasian lynx (Lynx lynx) in the French Jura and Vosges mountains. We sampled a total of 413 camera trapping sites (with 2 cameras per site) between January 2011 and April 2016 in seven study areas across seven counties of the French Jura and Vosges mountains. We obtained 592 lynx detections over 19,035 trap days in the Jura mountain and 0 detection over 6,804 trap days in the Vosges mountain. Based on coat patterns, we identified a total number of 92 unique individuals from photographs, including 16 females, 13 males and 63 individuals of unknown sex. Using spatial capture-recapture (SCR) models, we estimated abundance in the study areas between 5 (SE = 0.1) and 29 (0.2) lynx and density between 0.24 (SE = 0.02) and 0.91 (SE = 0.03) lynx per 100 km2. We also provide a comparison with non-spatial density estimates and discuss the expected discrepancies. Our study is yet another example of the advantage of combining SCR methods and non-invasive sampling techniques to estimate density for elusive and wide-ranging species, like large carnivores. While the estimated densities in the French Jura mountain are comparable to other lynx populations in Europe, the fact that we detected no lynx in the Vosges mountain is alarming. Connectivity should be encouraged between the French Jura mountain, the Vosges mountain and the Palatinate Forest in Germany where a reintroduction program is currently ongoing. Our density estimates will help in setting a baseline conservation status for the lynx population in France.

Biotic Interactions and Community Structure

A general pattern emerges from studies of subterranean communities. At a regional scale, hydrogeological and historical factors exert a controlling influence on many species, and the importance of species interactions is small. This is the pattern of the Jura Mountain groundwater communities. At a smaller geographical scale, there is little variation in hydrogeological or historical factors. For example, in both the Slovenian epikarst and Lyon aquifer studies, there was little if any variation in hydrogeological or historical factors. Species did differ in their occurrence along physicochemical axes, and these differences may well be the result of competition. Finally, some intensively studied communities show high levels of competition and predation, so strong that divergence rather than convergence occurs. There remains a gap between these somewhat unusual species combinations (beetles and cricket eggs, Appalachian cave stream invertebrates, Dinaric Niphargus, Australian calcrete diving beetles) and the broader scale community studies.